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Citations for Miltenyi Biotec :
51 - 100 of 126 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL, Miltenyi Biotec). The expansion factor was calculated based on peptide-loaded HLA class I tetramer staining as described before14 ...
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bioRxiv - Cell Biology 2020Quote: ... ESCs culture medium consists of N2B27 medium (Life technology) supplemented with PD0325901 (PD, 1 μM, Miltenyi Biotec), CHIR99021 (CH ...
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bioRxiv - Microbiology 2021Quote: ... Gametocytes were purified from culture at the required development stage using magnetic columns (LS columns, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... iMS1 and iMono cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Microbiology 2022Quote: ... mature parasite cultures (6-8% parasitemia) were magnetically separated using magnetic columns (MACS LD columns, Miltenyi Biotec) and diluted to 1% parasitemia containing 0.5 mL fresh erythrocytes88 ...
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bioRxiv - Immunology 2022Quote: Excised tumors (~300 mg) were minced using scissors in RPMI media containing enzyme cocktail mix from Miltenyi Biotec mouse tumor dissociation kit (Miltenyi Biotec ...
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bioRxiv - Biophysics 2024Quote: ... Cells were cultured in TexMACS media with IL-7 and IL-15 (Miltenyi Biotec; North Rhine-Westphalia, Germany). Cells were then cryopreserved until experimentation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were suspended in 0.5-1.0mL staining media and incubated with 2μl/bone anti-CD117 (cKit) magnetic beads (Miltenyi Biotec) for 30 minutes on ice ...
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bioRxiv - Molecular Biology 2020Quote: Primary cardiac cultures were isolated from P7 mice using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) using the gentleMACS homogenizer ...
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bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
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bioRxiv - Cell Biology 2024Quote: At specified timepoints the confluent cell cultures were enzymatically dissociated using Multi Tissue Dissociation Kit 3 (Miltenyi Biotec) and replated at 10.000 cells/cm2 in RPMI +B27 +Ins media with ROCK inhibitor Y27632 (5 µM ...
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bioRxiv - Immunology 2024Quote: Dead cells were removed from culture using the Dead Cell Removal Kit (Miltenyi Biotec, cat. 130-090-101). Then ...
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bioRxiv - Genomics 2021Quote: ... ESC were adapted to 2i media (serum-free N2B27 medium supplemented with MEK inhibitor PD0325901 [0.4 μM, Miltenyi Biotec] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... transferred in gentleMACS C-tubes containing RPMI/DMEM media and kit enzymes (tumor dissociation kit, Miltenyi Biotec, Gladbach, Germany) and were broken down using the gentleMACS ...
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bioRxiv - Developmental Biology 2020Quote: Neonatal primary cardiac cultures were isolated from P0 pups with a neonatal dissociation kit (Miltenyi Biotec, 130-098-373) and homogenizer (gentleMACS) ...
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bioRxiv - Neuroscience 2020Quote: ... The same method was adjusted to obtain oligodendrocyte cultures using anti-O4-MicroBeads (Miltenyi Biotec, Cat# 130-096-670). The immortalized mouse astrocyte line C8D30 (ATCC ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Synthetic Biology 2023Quote: ... Overnight cultures were diluted in 1:100 in DPBS and analyzed on a MACSQuant VYB flow cytometer (Miltenyi Biotec) using a previously described strategy to gate for single cells11 ...
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bioRxiv - Genomics 2021Quote: ... we used NS medium containing media hormone mix (MHM) medium (KOHJIN-Bio) supplemented with 2% MACS NeuroBrew-21 (Miltenyi Biotec), 20 ng/ml basic FGF (PEPROTECH) ...
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bioRxiv - Genomics 2023Quote: Human induced pluripotent stem cell lines were routinely cultured on Matrigel-coated dishes in MACS iPS-Brew media (Miltenyi Biotec) and passaged using ReLESR (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... media with 3% heat-inactivated human serum (Seralab, Sussex, UK) and 20 ng/mL human recombinant IL-2 (Miltenyi Biotec). BE-CAR7 products and CART19 control cells were made as previously described using pCCL-CAR7 or pTT-CAR19 vector ...
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bioRxiv - Cell Biology 2020Quote: ... The iPSC lines were cultured on tissue culture plastic coated with vitronectin (0.5 µg/cm2) in StemMACS iPS-Brew XF (Miltenyi Biotec). Pluripotency was validated by three lineage differentiation potential and Epi-Pluri-Score analysis ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Late-stage Dd2 parasites in RBCs were magnetically purified from 5% sorbitol-synchronized cultures using MACS LD columns (Miltenyi Biotec) and seeded at 0.8-million RBCs/well in a Seahorse miniplate which was precoated with Cell-Tak cell and tissue adhesive (Corning) ...
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPLEX Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPlex Cytokine 10 kit (Miltenyi Biotec). Also ...
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bioRxiv - Pathology 2024Quote: ... Cultures were negatively immunoselected for hematopoietic cells using CD45 and CD11b microbeads (Miltenyi Biotec, 130-052-301 & 130-097-142) and the autoMACS Pro Separator following the manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: The obtained homogenate was filtered in 3-5 ml of Wash Buffer (1x DPBS, containing 10% FBS, 20mM HEPES, and 6mM glucose) through a prewetted 70μm cell culture filter (Miltenyi Biotec) and centrifuged for 5 minutes at 300xg (4 °C).
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bioRxiv - Genetics 2023Quote: ... HSCs were harvest from the culture supernatant and were purified by MACS using CD34 MicroBeads (Miltenyi Biotec, 130-046-702).
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bioRxiv - Microbiology 2023Quote: An asynchronous parasite culture was washed in RPMI medium and then passed through a MACS LD magnet column (Miltenyi Biotec). NF54attB parasites complete a replication cycle in 44 to 48h under our culture condition and older parasites (>28h old ...
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bioRxiv - Cancer Biology 2024Quote: ... M-MDSCs expanded from bone marrow were collected (see “Bone Marrow Culture”) and sorted via magnetic bead isolation (Miltenyi Biotec) based on manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... where NCSCs were positively isolated from the mixed culture using p75-conjugated magnetic beads (catalogue#: 130-097-127, Miltenyi Biotec). Day 15 cells were collected with Accutase ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
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bioRxiv - Cell Biology 2022Quote: iPSCs were maintained in a feeder-free format on Geltrex-coated 6-well plates using StemMACS iPS-Brew XF media (Miltenyi Biotec) with daily feeding ...
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bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
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bioRxiv - Cell Biology 2024Quote: ... where NM is a mixture of 50% [v/v] DMEM:F12 + 50% [v/v] MACS Neuro Media (Miltenyi Biotec 130-093-570) + 0.5x GlutaMax (Thermo 35050061 ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in CTL test media (Immunospot) and incubated at 37 °C for 2 hours with either no stimulation or S1 peptide simulation (Miltenyi Biotec) at a final concentration of 2 µg/mL before adding GolgiPlug to all samples for 4 additional hours ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... Human blood samples were enriched for T cells using negative bead selection (StemCell) and stimulated in vitro in media containing a 1:100 titer of TransAct (Miltenyi Biotec), 5% FBS and 100 IU/mL of rhIL-2 ...
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bioRxiv - Immunology 2024Quote: ... lungs of infected mice were harvested and homogenized in 1 mL of minimal essential media with 10% bovine serum albumin using a gentleMACS C Tube (Miltenyi Biotec). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... erythrocytes infected with NF54 wild type parasites (500µl packed red blood cells in culture, 6% parasitemia) were purified using magnetic activated cell sorting (QuadroMACS™, Miltenyi Biotec). Purified late stages were accompanied with 30 µl uninfected red blood cells and cultured for 4h to let the cells recover ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Microbiology 2021Quote: ... cultures of the two parasite lines for comparison were (i) synchronized to 80% late schizonts using MACS purification columns (Miltenyi Biotec) (ii ...
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bioRxiv - Cancer Biology 2022Quote: ... MDSC enriched bone marrow cells were collected from culture as described above and subjected to M-MDSC magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Immunology 2022Quote: ... Cell suspension was diluted with warm complete culture medium and incubated in a continuous motion using the MACSmix rotator (Miltenyi Biotec) for 45 min at 37°C (5% CO2 ...
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bioRxiv - Synthetic Biology 2024Quote: The mixed mouse primary neonatal cardiomyocyte cell culture was obtained using a neonatal heart dissociation kit (Miltenyi Biotec, 130-098-373) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... in 96-well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). CD34+ HSPCs isolated from the BM of xenografted mice sacrificed between weeks 1-5 after grafting were directly seeded by FACS in the 96-well plates and cultured for 7 or 14 days before quantification of lineage outputs ...
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bioRxiv - Immunology 2022Quote: ... in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). UCB or BM CD34+ HSPC subsets were directly seeded by FACS in the corresponding plates and cultured for 10 (bulk cultures ...
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bioRxiv - Bioengineering 2023Quote: ... adjusted at 5×105 cells/ml and cultured 2-5 additional days in cell culture medium supplemented with 20 ng/ml IL-2 (Miltenyi Biotec) before being used in the experiments.