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Citations for Miltenyi Biotec :
51 - 93 of 93 citations for Influenza Virus Hemagglutinin HA H7N9 Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by 50 µl Anti-HA MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) after incubation for 2.5 h at 4 °C while slowly rotating ...
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bioRxiv - Molecular Biology 2021Quote: The co-immunoprecipitations were performed using µMACS HA or GFP Isolation Kit (Miltenyi Biotec) according to manufacturer protocol using 10×106 cells and 50 µL of antibody-coupled magnetic beads in a volume of 1 mL ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples resuspended in 2% FBS/PBS solution were blocked with mouse Fc Block (Miltenyi Biotec) for 5 minutes on ice ...
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bioRxiv - Cell Biology 2022Quote: ... + 5 µL DNase + 0.5% heat-inactivated FCS) with 10 µL FcR blocking reagent (Miltenyi Biotec), 10 µL biotin labeled anti-CD45 antibody (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked for 5 min at RT using FcR-blocking reagent (Miltenyi Biotec) and then incubated with fluorochrome-conjugated surface antibodies for 15 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended and incubated in MACS buffer containing Fc blocker and CD11c MicroBeads (Miltenyi Biotec) according to manufacturer instruction ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10 % heat-inactivated FCS and 50 ng/ml GM-CSF (130-093-862; Miltenyi Biotec). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere for 6 days and the medium was replaced at day 3 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Cell Biology 2021Quote: ... either MNCs or CD34 enriched cells were thawed and washed and stained with FC block (Miltenyi Biotec) for 10 min and washed again ...
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bioRxiv - Immunology 2023Quote: ... Livers sections were washed again and blocked using 1% Fc Block (MACS, Miltenyi Biotec, Auburn, CA, USA) and 5% BSA in HBSS during 1 hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... with Fc receptors blocking prior to Anti-ACSA-2 MicroBeads (Cat# 130-097-678, Miltenyi Biotec, Germany). Incubation was carried out for 15 minutes in the refrigerator at 4℃ ...
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bioRxiv - Plant Biology 2020Quote: ... for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec) for co-immunoprecipitation with PB1CP-HA ...
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bioRxiv - Plant Biology 2020Quote: ... Co-immunoprecipitation was conducted using the μMACS™ Epitope Tag Protein Isolation Kits according to manufacturer’s instructions (Miltenyi Biotec), and purified on magnetic μ Column (Miltenyi Biotec) ...
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bioRxiv - Plant Biology 2019Quote: ... RPS5A::mCherry-PDK1.2 were used for Co-IP assays using a µMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec), with RPS5A::mCherry-PDK1.1 and RPS5A::mCherry-PDK1.2 transgenic lines as controls ...
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bioRxiv - Plant Biology 2022Quote: ... Soluble supernatants were mixed with 25 μL of anti-HA or anti-MYC conjugated magnetic beads (Miltenyi Biotec) and incubated for 2 h with constant rotation at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mg of extract was incubated with µMACS MicroBeads conjugated to an anti-HA monoclonal antibody (Miltenyi Biotec) and beads were then captured on µMACS M-colums ...
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bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitation of uL18L1-3HA and uL18L8-3HA were performed using the μMACS HA-Tagged Protein Isolation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... cells were surface-labelled with an APC-conjugated anti-HA antibody (Miltenyi Biotec, Cat# 130-098-404, 1:50) and sorted by flow cytometry ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed twice with PBS 2% FCS before analysis by flow cytometry using a MACSQuant cytometer (Miltenyi Biotec, Germany) and analysis was done with the software FlowJo (vers 7.6).
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was performed on 500µl blood samples from healthy donors with or without Fc fragment receptor blockers (Miltenyi Biotec). Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
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bioRxiv - Immunology 2021Quote: ... bone marrow of RBD-his tag protein immunized mice were isolated and purified with mouse CD138 MicroBeads (Miltenyi Biotec, 130-098-257) following standard protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... The remaining supernatants were adjusted so that they had the same amount of total protein and mixed with 25 μL α-FLAG magnetic bead as instructed in the μMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec). From here ...
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bioRxiv - Immunology 2021Quote: ... the cells were resuspended in PBS containing 2% FCS and 2mM EDTA and stained with the extracellular mix using APC-Vio770 anti-CD14 (clone REA599) from Miltenyi Biotec and used at 1/100 ...
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bioRxiv - Immunology 2019Quote: ... (at 1:20-1:100 dilutions depending on the antibody) in the presence of human Fc receptor blocking reagent (1:10) (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... ACVR1-binding antibodies were enriched by incubation of biotinylated ACVR1-Fc and Myc-His monomeric ACVR1 at different concentrations with antibody expressing yeast cells followed by magnetic bead selection (Miltenyi Biotec) or flow cytometry on an Aria II cell sorter (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... and rinsed once with PBS and blocked in blocking buffer (PBS with 50% FCS, FcR Blocking Reagent 1:150 (Miltenyi Biotec) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Microbiology 2024Quote: ... Samples were divided into equal parts (250 μL each) and incubated with 50 μL of magnetic microparticles coated with monoclonal HA or MYC antibodies (MACS purification system, Miltenyi Biotec) at 4°C for 1 hour under rotation (10 rpm) ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant containing the resuspended proteins was used for immunoprecipitation assay using anti-GFP microbeads provided in the μMACS Epitope Tag Protein Isolation Kit according to the manufacturer’s protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). Approximately ...
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bioRxiv - Biophysics 2023Quote: ... buffer (2 mM EDTA and 0.5% fetal calf serum (FCS)) and microglia were isolated by positive selection using CD11b microbeads (Miltenyi Biotec, Gladbach, Germany), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and monocytes were magnetically sorted with magnetic microbeads coupled with antibodies directed against CD14 (Miltenyi Biotec #130-050-201). Monocytes were then seeded on glass coverslips at 1.5×106 cells/well in six-well plates in RPMI 1640 (Gibco ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by adding 150 ul of α-c-HA magnetic beads (µMACS Anti-c-myc Miltenyi Biotec, cat# 130-094-255) as described above ...
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bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... chips were similarly incubated with primary antibodies diluted in PBS containing 1% FBS and Fc Block (1:20 dilution; Miltenyi Biotec, 130-059-901) overnight at RT ...