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Citations for Miltenyi Biotec :
51 - 100 of 103 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... according to the manufacturer’s instructions and separated by the autoMACS™ Pro Separator (Miltenyi Biotec). FACS staining and treatment for viability assays of AML blasts was executed immediately after isolation ...
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bioRxiv - Plant Biology 2024Quote: ... and then with antibody (1:5000 anti-His-HRP (130-092-785, Miltenyi Biotec) or anti-FLAG-HRP (Ab49763 ...
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bioRxiv - Immunology 2020Quote: ... DP and CD8SP thymocytes were depleted by applying the “depleteS” program of AutoMACS Pro (Miltenyi Biotec). Enriched CD4SP thymocytes were then stained with CD4-Pacific blue (RM4-5 ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining 500 µL was processed on the autoMACS Pro Separator (#130-092-545, Miltenyi Biotec). After priming the autoMACS instrument ...
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bioRxiv - Cell Biology 2023Quote: ... MACS was performed applying an autoMACS® Pro Separator using autoMACS® columns (both Miltenyi Biotec) and the depletion program with a flow rate of 0.25 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... The CD73 positive cells were collected as photoreceptor cells using an autoMACS Pro Separator (Miltenyi Biotec, USA.).
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bioRxiv - Immunology 2020Quote: ... pro-B cells were positively selected from bone marrow using anti-CD19-labeled magnetic beads (Miltenyi Biotec) and were cultured for 24h hr in Opti-MEM supplemented with 15% FBS (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... Glycophorin A+ cells were enriched using CD235a Microbeads and positive selection with autoMACS Pro Separator (Miltenyi Biotec). Automated RNA extraction was performed using QIAcube Connect with RNeasy Micro Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with Anti-His-PE (clone: GG11-8F3.5.1; Miltenyi Biotec; Cat. #130-120-787) for 30 min at 4°C ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... For the Hi-C experiment Kit positive cells were magnetically separated (Miltenyi Biotec, 130-097-146).
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bioRxiv - Cancer Biology 2024Quote: ... and analyzed with an APC-conjugated anti-His monoclonal antibody (mAb, Miltenyi Biotec, Bergisch Gladbach, Germany) by flow cytometry ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell surface-bound LiTE TCE were detected using an APC-conjugated anti-His mAb (Miltenyi Biotec). Cell acquisition was performed using the BD FACSCanto™ II or BD LSRFortessa™ flow cytometers using BD FACSDiva software (BD Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: ... This was followed by the isolation of CPM+ lung progenitor cells using an autoMACS Pro Separator (Miltenyi Biotec) for the subsequent experiments.
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bioRxiv - Microbiology 2024Quote: ... stained with anti-His antibody (Antibody and Reagents Table) followed by anti-mouse-IgG magnetic beads (Miltenyi Biotec), and separated by MACS (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... Cells from BRAFi (n = 5) or BRAFi/C-Me (n = 6) tumors were pooled and CD45+ cells were isolated using CD45 MicroBeads (mouse, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 femora and 2 humeri was harvested in PBS/2.5% FBS and pooled before lineage negative depletion using autoMACS Pro separator (Miltenyi Biotec). Cells were incubated with a lineage antibody cocktail (B220 ...
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bioRxiv - Neuroscience 2022Quote: ... Image sections with a step size of 10 μm were generated by Imspector Pro software (v7.1.15, Miltenyi Biotec Gmbh, Germany) and stitched together using the implemented TeraStitcher script (v.9) ...
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bioRxiv - Microbiology 2024Quote: ... and a D column (Miltenyi Biotec, 130-041-201). The samples of ∼95-100% pure trophozoite-infected erythrocytes were plated at 0.2% hematocrit in standard culture media and incubated for a one-hour recovery period ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then washed and filtered through a 70μm cell strainer (catalog no. 352350, Falcon) prior to lineage depletion using the AutoMACS Pro (Miltenyi Biotec, USA). After depletion the negative fraction was resuspended in an antibody mix containing antibodies against Streptavidin-TxRed (1:200) ...
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bioRxiv - Cancer Biology 2024Quote: CD34+ cells were enriched from BM MNCs of sex-matched SF3B1-mutated MDS patients (n = 3) and healthy donors (n = 2) using CD34 Microbeads and positive selection with autoMACS Pro Separator (Miltenyi Biotec). 4000 or 7500 cells were plated as duplicates in MethoCult (H4434 ...
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bioRxiv - Cancer Biology 2024Quote: Lineage-negative BM cells (hereafter referred to as HPCs) were separated by the autoMACS® Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany), with depletion of lineage-positive cells using anti-biotin–conjugated magnetic beads (130-090-485 ...
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bioRxiv - Biochemistry 2021Quote: ... ACVR1-binding antibodies were enriched by incubation of biotinylated ACVR1-Fc and Myc-His monomeric ACVR1 at different concentrations with antibody expressing yeast cells followed by magnetic bead selection (Miltenyi Biotec) or flow cytometry on an Aria II cell sorter (BD Biosciences ...
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bioRxiv - Plant Biology 2024Quote: ... Protein purity was verified by protein gel electrophoresis followed by coomassie staining and western blotting using anti-His (HRP) antibody (Miltenyi Biotec). Signals were generated by chemiluminescence using Clarity ECL Western Blotting Substrate (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... enzyme R and enzyme D (Multi Tissue Dissociation Kits, Miltenyi Biotec) and dissociated using the gentleMACSTM Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... bone marrow of RBD-his tag protein immunized mice were isolated and purified with mouse CD138 MicroBeads (Miltenyi Biotec, 130-098-257) following standard protocol provided by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... and used for eosinophil purifications by negative selection on an AutoMACS Pro separator using the Eosinophil Purification Kit (Cat. No. 130-092-010, Miltenyi Biotec, Bergisch Gladbach, Germany). Eosinophil purity was >98% for all samples as determined by counting a minimum of 300 cells on cytospin slides stained with Shandon™ Diff-Kwik™ stains (Cat ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cell suspension was treated with 7-aminoactinomycin D (7-AAD; Miltenyi Biotec) and incubate for 10 min on ice for viability staining prior to cell sorting ...
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bioRxiv - Cell Biology 2021Quote: Different lineages of in vitro cultured endMSCs (n = 3) were primed using a combination of recombinant pro-inflammatory cytokines and Toll-like receptors (TLR) ligands: IFNγ (Miltenyi Biotec Inc, San Diego, CA, USA) at 1 ng/ml and 100 ng/ml ...
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bioRxiv - Microbiology 2024Quote: Gametocyte-infected erythrocytes were purified using MACS D columns (Miltenyi Biotec; #130-041- 201) in combination with a SuperMACS™ II Separator (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2021Quote: Filtered cells were diluted 10x in 0.5%BSA in D-PBS (#130-091-376, Miltenyi Biotec) prior to cell counting on a MACSQuant Analyzer 10 (#130-096-343 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended in 1 mL 0.5% BSA in D-PBS (#130-091-376, Miltenyi Biotec) and filtered through a 35 µm filter (#352235 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 1 mL 0.5% BSA in D-PBS (#130-091-376, Miltenyi Biotec) and filtered through a 35 µm filter (#352235 ...
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bioRxiv - Immunology 2020Quote: ... iMS1 and iMono cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Genomics 2024Quote: PitNET samples were dissociated with 8-10 ul of enzyme D Multi Tissue Dissociation Kit (Miltenyi Biotec) or 2 mg/ml collagenase IV (Gibco ...
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bioRxiv - Genomics 2024Quote: Thyroid carcinoma samples were dissociated with 30-35 ul of enzyme D Multi Tissue Dissociation Kit (Miltenyi Biotec) or 2 mg/ml collagenase IV (Gibco ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Neuroscience 2021Quote: ... washed with 1x D-PBS and treated with TrypLE Express before being dissociated with the gentleMAC Octo Dissociator (Miltenyi Biotec) at 37°C to create a single cell suspension ...
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bioRxiv - Neuroscience 2021Quote: Cells were pelleted at 300 x g for 10 minutes at 4ºC and resuspended in 90 µL of 0.5% BSA in D-PBS with 10 µL of CD11b (Microglia) MicroBeads (#130-093-636, Miltenyi Biotec) per 107 total cells ...
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bioRxiv - Genomics 2024Quote: Adrenal gland neoplasm samples were dissociated with 25-30 ul of enzyme D Multi Tissue Dissociation Kit (MTDK) (Miltenyi Biotec) or enzyme A Neural Tissue Dissociation Kit P (NTDK ...
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bioRxiv - Neuroscience 2023Quote: Hippocampal cells from C57BL6/N mice were pelleted at 300 x g for 10 minutes at 4°C then resuspended in 90 µL of 0.5% BSA in D-PBS and 10 µL of CD11b (Microglia) MicroBeads (#130- 093-634, Miltenyi Biotec) followed by thorough mixing ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal single cell suspensions were pelleted at 300 × g for 10□min at 4°C and resuspended in 90□μl of 0.5% BSA in D-PBS with 10□μl of CD11b (Microglia) MicroBeads (#130-093-636, Miltenyi Biotec) per 107 total cells ...
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bioRxiv - Cancer Biology 2023Quote: ... were cut into small pieces and digested with in 2.4 mL of dissociation solution [DMEM medium supplemented with enzyme mixture (100ul Enzyme D, 50uL Enzyme R and 12.5uL Enzyme A)] using Tumor Dissociation Kit (Miltenyi Biotec). After incubation for 45 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the pellet resuspended in 500 µl of D-PBS–0.5% BSA and then added to pre-wetted LS columns (Miltenyi Biotec). The columns were washed with 3 × 3 ml of D-PBS–0.5% BSA buffer after which the cells were flushed out using 5 ml of D-PBS–0.5% BSA buffer ...
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bioRxiv - Neuroscience 2022Quote: ... On day five ECs were dissociated with StemPro Accutase (diluted 1:6 in D-PBS) and separated via Magnetic-activated cell sorting (MACS, Miltenyi Biotec) using CD34 MicroBeads ...
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bioRxiv - Pathology 2024Quote: ... Isotype controls or fluorescence minus one (FMO) controls were used, and the live/dead marker 7-aminoactinomycin D (7-AAD, PerCP) (Miltenyi Biotec) was added to all samples 10 min before analysis (10 μl to 1 ml of cell suspension).
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bioRxiv - Genomics 2023Quote: ... Dissociated cells of each slice were collected with 6ml cold D-PBS and apply to a MACS SmartStrainer (70 μm, Miltenyi Biotec) placed on one well of a deepwell 24-well plate following centrifugation at 300×g for 10 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... stimulated with IL-2 (R&D), and/or T cell differentiating cytokines and antibodies (Th1: anti-IL-4, IL-12 (Miltenyi Biotec), Treg ...
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bioRxiv - Immunology 2020Quote: ... Skin T cells from the breast and abdomen were isolated from punch biopsies following a standardized digestion protocol with the gentleMACS™ Dissociator and the whole skin dissociation kit containing enzyme A and D (both Miltenyi Biotec) before FACS-sorting for live (Hoechst 33258 negative or propidium iodide negative ...
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bioRxiv - Microbiology 2023Quote: Parasites were enriched from ∼100 mL of culture containing synchronized schizonts (∼36-44 h post-invasion) at ∼10% parasitemia using a SuperMACS™ II Separator and a D Column (Miltenyi Biotec). Numbers of eluted cells were determined with a hemocytometer and parasitemia was calculated from a Giemsa-stained smear ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was gently re-suspended in 3100 μl cold D-PBS and processed for additional removal of myelin and cell debris with Debris Removal Solution (Miltenyi Biotec, cat. no. 130-109-398), following manufacturer’s instructions ...