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Citations for Miltenyi Biotec :
51 - 72 of 72 citations for D Galactose U 13C6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 0.5× N-2 MAX Media Supplement (R and D systems, AR009), 100 U/ml penicillin/streptomycin (Nacalai, 26253-84) and 0.5× MACS NeuroBrew21 supplement without vitamin A (Miltenyi Biotec, 130-097-263)) for 8 days replaced the media every two to three days ...
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bioRxiv - Neuroscience 2021Quote: ... washed with 1x D-PBS and treated with TrypLE Express before being dissociated with the gentleMAC Octo Dissociator (Miltenyi Biotec) at 37°C to create a single cell suspension ...
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bioRxiv - Neuroscience 2021Quote: Cells were pelleted at 300 x g for 10 minutes at 4ºC and resuspended in 90 µL of 0.5% BSA in D-PBS with 10 µL of CD11b (Microglia) MicroBeads (#130-093-636, Miltenyi Biotec) per 107 total cells ...
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bioRxiv - Neuroscience 2023Quote: Hippocampal cells from C57BL6/N mice were pelleted at 300 x g for 10 minutes at 4°C then resuspended in 90 µL of 0.5% BSA in D-PBS and 10 µL of CD11b (Microglia) MicroBeads (#130- 093-634, Miltenyi Biotec) followed by thorough mixing ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal single cell suspensions were pelleted at 300 × g for 10□min at 4°C and resuspended in 90□μl of 0.5% BSA in D-PBS with 10□μl of CD11b (Microglia) MicroBeads (#130-093-636, Miltenyi Biotec) per 107 total cells ...
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bioRxiv - Genomics 2024Quote: Adrenal gland neoplasm samples were dissociated with 25-30 ul of enzyme D Multi Tissue Dissociation Kit (MTDK) (Miltenyi Biotec) or enzyme A Neural Tissue Dissociation Kit P (NTDK ...
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bioRxiv - Cancer Biology 2023Quote: ... were cut into small pieces and digested with in 2.4 mL of dissociation solution [DMEM medium supplemented with enzyme mixture (100ul Enzyme D, 50uL Enzyme R and 12.5uL Enzyme A)] using Tumor Dissociation Kit (Miltenyi Biotec). After incubation for 45 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the pellet resuspended in 500 µl of D-PBS–0.5% BSA and then added to pre-wetted LS columns (Miltenyi Biotec). The columns were washed with 3 × 3 ml of D-PBS–0.5% BSA buffer after which the cells were flushed out using 5 ml of D-PBS–0.5% BSA buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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Effective targeting of CD19 positive primary B-ALL cells using CAR-NK cells generated with mRNA-LNPsbioRxiv - Cancer Biology 2024Quote: ... were stimulated with irradiated (100 Gy) EBV-LCL (20:1 feeder cell:NK ratio) and recombinant human IL-2 (500 U/mL; Miltenyi Biotec, Inc., San Diego, CA) and IL-15(10ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... At the end of the incubation the single cell suspension was washed with 500 μL wash buffer (0.02% BSA in 1X PBS with 80 U/mL Protector RNase Inhibitor) and passed through a 20 μM filter (MACS, Miltenyi Biotec cat#130-101-812) to a fresh 2 mL Protein LoBind Eppendorf ...
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bioRxiv - Cancer Biology 2024Quote: ... TL-1 cells were cultured in RPMI-1640 with 10% FBS and supplemented with 200 U/mL IL-2 (Miltenyi Biotec cat # 130-097-743) and NKL cells were cultured in RPMI-1640 with 10% FBS and supplemented with 100 U/mL IL-2.
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bioRxiv - Neuroscience 2022Quote: ... On day five ECs were dissociated with StemPro Accutase (diluted 1:6 in D-PBS) and separated via Magnetic-activated cell sorting (MACS, Miltenyi Biotec) using CD34 MicroBeads ...
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bioRxiv - Pathology 2024Quote: ... Isotype controls or fluorescence minus one (FMO) controls were used, and the live/dead marker 7-aminoactinomycin D (7-AAD, PerCP) (Miltenyi Biotec) was added to all samples 10 min before analysis (10 μl to 1 ml of cell suspension).
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bioRxiv - Genomics 2023Quote: ... Dissociated cells of each slice were collected with 6ml cold D-PBS and apply to a MACS SmartStrainer (70 μm, Miltenyi Biotec) placed on one well of a deepwell 24-well plate following centrifugation at 300×g for 10 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... stimulated with IL-2 (R&D), and/or T cell differentiating cytokines and antibodies (Th1: anti-IL-4, IL-12 (Miltenyi Biotec), Treg ...
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bioRxiv - Immunology 2020Quote: ... Skin T cells from the breast and abdomen were isolated from punch biopsies following a standardized digestion protocol with the gentleMACS™ Dissociator and the whole skin dissociation kit containing enzyme A and D (both Miltenyi Biotec) before FACS-sorting for live (Hoechst 33258 negative or propidium iodide negative ...
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bioRxiv - Microbiology 2023Quote: Parasites were enriched from ∼100 mL of culture containing synchronized schizonts (∼36-44 h post-invasion) at ∼10% parasitemia using a SuperMACS™ II Separator and a D Column (Miltenyi Biotec). Numbers of eluted cells were determined with a hemocytometer and parasitemia was calculated from a Giemsa-stained smear ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was gently re-suspended in 3100 μl cold D-PBS and processed for additional removal of myelin and cell debris with Debris Removal Solution (Miltenyi Biotec, cat. no. 130-109-398), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 minutes at 4°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellets were resuspended in 100 µl cold D-PBS and stained with fluorochrome-conjugated antibodies recognizing human CD11b and GFAP (Miltenyi Biotec, 130-113-810 and 130-123-846) as described above ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA-2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 min at 4°C in the dark ...