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Citations for Miltenyi Biotec :
901 - 950 of 1049 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: CD34+ HSPC were enriched from cord blood mononuclear cells (MNC) by magnetic selection using anti-CD34 conjugated magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec, 130-100-453). To obtain a sufficient cell number for ImageStream and Western blot analysis ...
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bioRxiv - Neuroscience 2023Quote: Human tumor tissue samples were acquired during neurosurgical biopsy or open resection and stored in Tissue Storage Solution (Miltenyi Biotec, 130-100-008) for 2-30 hours until further processing for scRadiotracing ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells ...
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bioRxiv - Molecular Biology 2024Quote: ... The suspension was homogenized in a Dounce (loose pestle, 20 strokes) homogenizers and filtered through 100 µm MACS SmartStrainers (Miltenyi Biotec, United States). After incubation on ice for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Primary monocytes and neutrophils were isolated from mouse bone marrow using negative isolation kits (Miltenyi Biotec, 130-100-629 and 130-097-658). Cells were cultured in RPMI 1640 media (monocytes ...
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bioRxiv - Neuroscience 2021Quote: ... microglia proliferation was induced by adding 5 ng/ml GM-CSF (Miltenyi Biotec, 130-095-746) to the complete medium ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130-093-387 and 130-093-375) and IL-2 (100 U/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130- 093-387 and 130-093-375), and IL-2 (100 U/mL ...
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bioRxiv - Biophysics 2019Quote: ... 5% CO2 in the presence of IL-2 (50⍰ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany) and used 6 to 10⍰days after stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... and 60 ng/mL of human receptor activator of NF-κB-ligand (RANK-L, Miltenyi Biotec). The medium was then changed every third day by RPMI containing 10% FCS ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 40 ng/ml basic fibroblast growth factor-2 (FGF2) (Miltenyi Biotec, Bergisch Gladbach, Germany), 20 ng/ml epidermal growth factor (EGF ...
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bioRxiv - Cell Biology 2020Quote: ... or 20 ng/ml PDGF-AA (human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec). Inhibition of PDGF-signaling was performed by addition of 1μM imatinib (Cat nb ...
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bioRxiv - Neuroscience 2020Quote: ... microglia proliferation was induced by adding 5 ng/ml GM-CSF (Miltenyi Biotec, 130-095-746) to the complete medium ...
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bioRxiv - Biophysics 2019Quote: ... 5% CO2 in the presence of IL-2 (50 ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany) and used 7 days after activation ...
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bioRxiv - Immunology 2020Quote: ... cells were blocked at 37C for 15 minutes with 0.5ug/mL anti-CD40 mAb (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... with 4000 cells/well in serum free medium (SFM) containing 10 ng/ml BMP4 (Miltenyi Biotec), 10 ng/ml bFGF (Peprotech) ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with growth factors (Miltenyi Biotec, IL-3, IL-6 and G-CSF (10 ng/mL), h-SCF and FLt3-L (50 ng/mL) ...
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bioRxiv - Immunology 2021Quote: ... 500U/ml Collagenase I) with a tissue-lyser (Gentle MACS, Miltenyi Biotec, Bergisch Gladbach, Germany, Germany) and digested enzymatically for 20-30 min at 37°C while continuously shaking in a water bath ...
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bioRxiv - Systems Biology 2021Quote: ... supplemented with growth factors (Miltenyi Biotec, IL-3, IL-6 and G-CSF (10 ng/mL), h-SCF and FLt3-L (50 ng/mL) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and cultured in the presence of 3 μg/mL anti-CD28 mAb (clone: 15E8, Miltenyi Biotec), 25 U/mL human IL-7 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... Sigma-Aldrich)-coated tissue culture treated 24 well plates and treated with IFN-γ (1ng/ml, Miltenyi Biotec) and TNFα (1ng/ml ...
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bioRxiv - Immunology 2024Quote: ... total bone marrow cells were cultured with GM-CSF (100ng/ml, Miltenyi Biotec, 130-095-742) in complete RPMI 1640 (RPMI 1640 + Glutamax ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells (used for in vivo experiments) were supplied with 0.5ng/ml IL15 (Miltenyi Biotec) and 10ng/µl IL7 (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... and human granulocyte-macrophage colony-stimulating factor (GM-CSF; 20ng/ml; MILTENYI BIOTEC; 130-093-866). At day 5 ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with growth factors (Miltenyi Biotec, IL-3, IL-6 and G-CSF (10 ng/mL), h-SCF and FLt3-L (50 ng/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... for monocyte-derived dendritic cells and 20 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) and 40 ng/ml M-CSF (#130-096-485 ...
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bioRxiv - Neuroscience 2023Quote: ... At d15 cells were treated with IL-4 (20 ng/ml, LOT130-094-117, Miltenyi Biotec), IL-6 (100 ng/ml ...
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bioRxiv - Neuroscience 2024Quote: ... in a six-well plate in 2 ml StemMACS iPS-Brew (Miltenyi Biotec, Bergisch Gladbach, Germany). Medium was supplemented with 100 U/ml penicilin/streptomycin (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... to the final concentration of 66.7 mg/ml (w/v) using a gentleMACS Dissociator (Miltenyi Biotec). Homogenized samples containing 50 mg tissue were directly frozen at -70 °C until extraction of DNA and metabolites.
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bioRxiv - Physiology 2024Quote: ... nuclei were stained with Hoechst 33342 (15 µg/mL; Miltenyi Biotec, cat no. 130-111-569) for 10 min at RT ...
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bioRxiv - Immunology 2024Quote: ... except that we used 20 ng/ml of recombinant murine GM-CSF (Peprotech or Miltenyi biotec) instead of M-CSF ...
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bioRxiv - Microbiology 2021Quote: ... tissue samples were weighed and placed in gentleMACS M tubes (30 mg in 100 μl PBS) and dissociated using a gentleMACS Tissue Dissociator (protein01 program)(Miltenyi Biotec B.V., Leiden, Netherlands). Next ...
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bioRxiv - Bioengineering 2022Quote: The cell pellet was then incubated in a 4 °C fridge with rocking for 15 minutes in 100 µL of MACS buffer and 25 µL of lineage depletion microbeads from the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The Lin− fraction of the CB cells and the AML cells were further enriched for CD34 with the human CD34 MicroBead Kit UltraPure (Miltenyi Biotec, 130-100-453), which contains microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype ...
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bioRxiv - Immunology 2022Quote: Dissected and trimmed tumors were washed in pre-cooled RNase-free H2O and transferred into MACS Tissue storage solution (Miltenyi Biotec, 130-100-008). Samples were shipped on ice overnight to Novogene ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Digestion was stopped with ice-cold DMEMF12 and the supernatant was sequentially filtered through a 100 μm cell strainer (Falcon, 352360) and a 30 μm cell strainer (Miltenyi Biotec, 130-098-458). The fraction retained above the 30 μm filter ...
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bioRxiv - Bioengineering 2024Quote: ... isolated mononuclear cells were positively selected for CD34 using the CD34+ Microbead kit Ultrapure (Miltenyi Biotec, San Diego, CA, USA, cat.: 130-100-453). Cells were cultured at 1.5×105–2.5×105 cells/mL in CellGenix® GMP Stem Cell Growth Medium (SCGM ...
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bioRxiv - Cancer Biology 2023Quote: ... and later monocytes were enriched from the total bone marrow cells using the Monocyte Isolation Kit (BM) from Miltenyi Biotec (Cat: 130-100-629) through a negative selection process following the manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... each culture was diluted 100-fold in a new plate containing cold M9-medium before being counted using a MACSQuant10 flow cytometer (Miltenyi biotec, Bergisch Gladbach, Germany). We conducted flow cytometry analysis with a moderate flow rate using SSC 1.4 as a trigger ...
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bioRxiv - Cancer Biology 2020Quote: Human CD34+ cells were purified from adult peripheral whole blood (LeukoPaks) by positive selection using microbeads magnetic cell separation system (Miltenyi Biotec, Cat # 130-100-453), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and then the cell pellet was resuspended in 100 µL of MACS buffer and 25 µL of anti-integrin α-7 microbeads (Miltenyi Biotec, #130-104-261) for incubation at 4 °C for 15 minutes ...
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bioRxiv - Neuroscience 2021Quote: Brain tissue samples were weighed and placed in gentleMACS M tubes (30 mg in 100 µl PBS) and treated using a gentleMACS Tissue Dissociator (protein01 program)(Miltenyi Biotec B.V., Leiden, The Netherlands). Next ...
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bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10 % heat-inactivated FCS and 50 ng/ml GM-CSF (130-093-862; Miltenyi Biotec). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere for 6 days and the medium was replaced at day 3 ...
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bioRxiv - Immunology 2021Quote: Splenocytes or lung leukocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 24 h ...
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bioRxiv - Immunology 2020Quote: ... and expanded for 14 days in complete RPMI culture medium containing rIL2 (20 IU/mL, Miltenyi Biotec). The expansion factor was calculated based on peptide-loaded HLA class I tetramer staining as described before14 ...
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bioRxiv - Immunology 2020Quote: ... in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14, Miltenyi Biotec). After 3 hrs of specific peptide stimulation ...
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bioRxiv - Immunology 2021Quote: ... Spleens were dissociated in 5 mL PBS using a gentleMACS™ dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Following centrifugation (800xg ...
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bioRxiv - Microbiology 2020Quote: ... 50 ng/ml recombinant human granulocyte macrophage colony–stimulating factor (rhGM-CSF) (130-093-865, Miltenyi Biotec), and 50 ng/ml rh interleukin (IL)-4 (130-093-921 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were cultured in presence of 3 μM CHIR-99021 and 20 ng/mL HGF (Miltenyi Biotec) for two days to obtain the GStemHep ...