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Citations for Miltenyi Biotec :
901 - 950 of 1056 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade AE CM240 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were incubated with APC-conjugated cytochrome c antibody (1: 50 dilution in permeabilisation buffer)(Miltenyi Biotec) and analyesd by FACS on an LSR-II flow cytometer (BD Biosciences).
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated with anti-APC magnetic beads (Miltenyi Biotec, Catalog No. 130-090-855, 1/4) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... rested overnight at a density of 1×107 cells/mL in 100 IU/mL IL-2 (Miltenyi Biotec)-supplemented medium ...
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bioRxiv - Neuroscience 2020Quote: ... Microglia were isolated from adult mouse brain using the multi tissue dissociation kit 1 (Miltenyi Biotec #130-110-201), followed by CD11b microglia isolation kit (Miltenyi Biotec #130-093-634 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then co-incubated with 1 μg of APC-conjugated anti-IgG antibody (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 μg of PE-conjugated anti-Flag antibody (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2020Quote: ... 30 μg was detected with horseradish peroxidase (HRP)-conjugated antibodies against GFP (Anti-GFP-HRP, 1:10000, Miltenyi Biotec) or anti-tubulin (mouse monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... Neonatal murine microglia were isolated from P0 CD-1 pups using CD11b microbeads (Ca #130-093-634, Miltenyi Biotec) and their purity was assessed by immunocytochemistry of myeloid cell markers (Iba-1 and CD11b ...
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bioRxiv - Cancer Biology 2022Quote: ... and then incubated with the surface antibody CD34-PE-Vio770 (clone AC136, lot #5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... cells were surface-labelled with an APC-conjugated anti-HA antibody (Miltenyi Biotec, Cat# 130-098-404, 1:50) and sorted by flow cytometry ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP [GG4-2C2.12.10] (1:5000; 130-091-833; Miltenyi Biotec), rabbit anti-EXOC1 antibody (1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: Mitochondria were isolated from WT and NSUN2 KO HEK293T cells (1 × 107) using the Mitochondria Isolation Kit (Miltenyi Biotec). Steady-state levels of subunit proteins of mitochondrial respiratory chain complexes were analyzed by immunoblotting with Total OXPHOS Rodent WB Antibody Cocktail (1:250 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1×107 cells per replicate were purified using 100ul of Pluripotent Stem Cell microbeads (Miltenyi Biotec 130-095-804), and LS columns (Miltenyi Biotec 130-042-401) ...
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bioRxiv - Neuroscience 2023Quote: ... antibody labeling and boosting step was performed with the addition of CD11b (1:50) (Miltenyi Biotec, 130-110-611) and GFP-Nanobooster LOT 102 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... added with 40μL of magnetic microbeads conjugated with anti-Thy-1 antibody (Miltenyi Biotec 130–049–101, Auburn, CA), and mixed well ...
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bioRxiv - Cell Biology 2023Quote: ... were used to deplete lineage+ (Lin+) cells and Anti-Sca-1 (non-HSC) MicroBeads (130-106-641, Miltenyi Biotec) for mice for enriching with Sca+ cells ...
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bioRxiv - Bioengineering 2023Quote: ... 25 µl of each sample was incubated with APC-labelled CD63 antibody (Miltenyi Biotec, Germany; 1 nM per well) overnight under dark conditions ...
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bioRxiv - Genomics 2023Quote: ... TB6505 and TB6545 (1 givinostat and 7 vehicle slices) were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec) with modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor cells were incubated 24 hours in R10 medium (i.e. supplemented or not with 200 ng·mL-1 IFNγ (Miltenyi Biotec)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Overnight cultures were diluted in 1:100 in DPBS and analyzed on a MACSQuant VYB flow cytometer (Miltenyi Biotec) using a previously described strategy to gate for single cells11 ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in MACS buffer (0.5% BSA and 1 mM EDTA in PBS, pH 7.4) and incubated with CD11b MicroBeads (Miltenyi Biotec) for 30 minutes to label the microglial cells ...
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bioRxiv - Cancer Biology 2024Quote: ... TYR or Mart-1/MLANA PepTivator peptide pools (15-mer peptide sequences with 11 amino acids overlap, Miltenyi Biotec) for 60 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was removed and 1 mL of wash buffer (PBS + 0.5% BSA (Miltenyi Biotec, Cat. # 130-091-376) + 1 U/uL of RNase inhibitor ...
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bioRxiv - Systems Biology 2019Quote: ... Next the pellet was incubated with 200 µL/1 × 107 total cells of dead cell removal paramagnetic microbeads (Miltenyi Biotec) and incubated for 15 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... for an additional 15 min and subsequently subjected to positive selection of Gr-1+ cells on a MACS LS column according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Neuroscience 2020Quote: ... The perfused brains were dissected and cut into small 1 mm3 pieces before homogenizing them in DPBS++ containing 0.4% DnaseI on the tissue dissociator (Miltenyi Biotec) at 37 °C for 35 mins ...
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bioRxiv - Cell Biology 2021Quote: ... The high pluripotent stem cells form colonies were selected by Anti-Tra-1-60-PE and Anti-PE MicroBeads following the manufacturer’s instructions (MACS Miltenyi Biotec). The stable colonies were frozen down at -80°C ...
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bioRxiv - Systems Biology 2020Quote: ... Cell suspensions (up to 1×108 cells/500 µL of Magnetic Bead Buffer) were applied onto MS columns (Miltenyi Biotec) in a magnetic field ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C for 1 hr and passed through a 70 μm and a 30 μm cell strainer (Miltenyi Biotec). CD45+ cells were isolated using magnetic beads (Miltenyi Biotec 130-052-301 ...
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bioRxiv - Bioengineering 2022Quote: ... for the cardiac marker Cardiac troponin T conjugated to APC (cTnT; 1:50; REA400; 130-120-543, Miltenyi Biotec, Germany), and cells were frozen in RPMI + B27C with 10 µM of RI + 10% FCS + 10% DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
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bioRxiv - Immunology 2019Quote: ... before culturing in a 1:10 ratio with 5×105 BALB/c splenic APCs (prepared via Thy1.2+ cell depletion by MACS, Miltenyi Biotec) in a 96-well round-bottom plate in complete RPMI medium for 5 days ...
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bioRxiv - Molecular Biology 2019Quote: ... and GR-1) negative cells were collected and then positively selected for CD117+ (cKit+) cells using CD117 microbead (Miltenyi Biotec) to yield Lineage− cKit+ (Lin−cKit+ ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse anti-Cy5/Alexa Fluor 647 (cocktail of antibodies at 1:100 each from Sigma-Aldrich [Cat# C1117, RRID:AB_477654] and Miltenyi Biotec [custom-ordered antibody used in their Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads ...
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bioRxiv - Immunology 2021Quote: ... OT-1 cells were purified from OT-1 transgenic mice using negative selection with CD8a+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2021Quote: ... The stroma was negatively selected against epithelium using a EPCAM antibody coated microbeads (1:10; Miltenyi Biotec #130-061-101) and a Miltenyi MS column.
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bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in sorting buffer and incubated with ACSA-2 MicroBeads (1:10, Miltenyi Biotec, 130-097-678) for 15 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Genetics 2023Quote: ... and the precipitated pellet was resuspended in 2.5 mL 1× Buffer and filtered with a 70 µM MACS SmartStrainer (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Genetics 2023Quote: ... 1 × 10∧7 cells were processed for dead cell depletion using Dead Cell Removal Kit (Miltenyi Biotec # 130-090-101) as per the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... GMCSF/IL-9 cytokine ratios of 1:2 diluted amniotic fluid were evaluated by the cytokine bead array (Miltenyi Biotec MACSPlex Cytokine 12 Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... or tumor necrosis factor-alpha (1 ng/mL, TNF-α; catalog no. 130-094-022, Miltenyi Biotec, Bergisch Gladbach, Germany) in combination with LPS ...
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bioRxiv - Systems Biology 2024Quote: ... tumor was cut into 1-2 mm pieces and transferred to a gentleMACS C Tube (Miltenyi Biotec #130-093-237) containing 4.7 mL of sterile media (Knockout DMEM/F12) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed three times and incubated in PBS containing DAPI (1 μg/mL) for 15 min before samples were processed using flow cytometry (MACSQuant, Miltenyi Biotec). At least 10000 events were analyzed per sample using FlowLogic software (Miltenyi Biotec).
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cultures were grown overnight at 37 °C and shaking and then diluted in 1:50 in DPBS and analyzed on a MACSQuant VYB flow cytometer (Miltenyi Biotec) using a previously described strategy to gate for single cells3 ...
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bioRxiv - Cancer Biology 2019Quote: ... whole lungs from mice bearing MDA-LM2 macrometastases (3 weeks post tail vein injection) were harvested and lysed in 1 ml Cell Lysis Buffer 2 (R&D) using the gentleMACS Dissociator (Miltenyi Biotec). As a control ...
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bioRxiv - Cell Biology 2019Quote: ... For immunophenotyping of MSCs the cells were stained with 1:50 dilution of fluorochrome-conjugated mouse monoclonal antibodies against CD73 (PE-Vio770, 130-104-224, Miltenyi Biotec), CD105 (APC ...
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bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...