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Citations for Miltenyi Biotec :
901 - 950 of 2724 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
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bioRxiv - Immunology 2021Quote: Murine naïve B cells were purified from spleen by magnetic negative selection by using Pan B Cell Isolation Kit (Miltenyi Biotec, 130-095-813) according to the manufacturer’s instructions and maintained in appropriate culture medium ...
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bioRxiv - Immunology 2020Quote: PBMCs were enriched for B cells by negative selection using a pan B cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1 X Phosphate-buffered Saline (PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... After an additional 24h co-cultured cells were trypsinized and endothelial cells were purified using CD31 microbead kit (Miltenyi Biotec #130-091-935) according to manufacturer instructions.
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bioRxiv - Immunology 2022Quote: ... were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Systems Biology 2023Quote: ... we further isolated the CD34+ hematopoietic stem cells (HSCs) using magnetic-activated cell sorting according to the manufacturer’s instructions (CD34 MicroBead Kit, Miltenyi Biotec #130-046-702). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... Peripheral mononuclear cells were collected by density gradient centrifugation and enriched using a CD8+ T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... then naïve CD4+ T cells (CD4+CD62L+CD44-) were isolated using the Miltenyi Naïve CD4+ T cell isolation kit (Miltenyi Biotec, 130-104-453), and followed by purity check by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... the negative fraction of CD8+ T cells selection was collected and incubated with the Tumor Cell Isolation Kit (Miltenyi Biotec, #130-108-339) for 15 min incubation at 4°C ...
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bioRxiv - Immunology 2023Quote: T2 and T3 cells were then depleted for CD45+ cells using the Miltenyi LD column prep kit (Miltenyi Biotec Inc., Charlestown, MA, USA). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Plasmacytoid dendritic cells were isolated by negative selection from prepared PBMCs with the Human Plasmacytoid Dendritic Cell Isolation Kit-II from Miltenyi Biotec (Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Single cell suspensions of DRG cells from BALB/cAnNRj mice were blocked with FcR blocking solution (Miltenyi Biotec l No.130-092-575) and stained with anti-CD45:PE (BioLegend 103105) ...
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bioRxiv - Cell Biology 2023Quote: PRP from human subjects was subjected to white blood cell (WBC) and red blood cell (RBC) depletion by using CD45 MicroBeads (for WBCs, #130-045-801, Miltenyi Biotec., Gaithersburg, MD) and CD235a (Glycophorin A ...
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bioRxiv - Immunology 2024Quote: ... from a cell suspension and harvested from the crushed spleens of OT-I mice using a CD8a+ T-Cell Isolation Kit and magnetic-associated cell sorting (MACS) according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). Similarly ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Lung single cell suspensions were subjected to CD4+ cell positive enrichment according to kit manufacturer’s instructions (Miltenyi Biotec, cat. no. 130-097-048) before fluorochrome staining ...
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bioRxiv - Immunology 2023Quote: ... The CD4+ T cells were then enriched by negative selection using a CD4 T cell isolation kit as per manufacturer’s instructions (Miltenyi Biotec; Bergisch Gladbach, Germany).
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed in MACS-buffer und T cells were MACS-sorted from suspension using a Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequently ...
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bioRxiv - Immunology 2022Quote: Primary human CD4-positive T cells were isolated from enriched peripheral blood mononuclear cell (PBMC) fractions using the human CD4+ T cell isolation kit from Miltenyi Biotec (Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Oligodendrocyte precursor cells (OPCs) were isolated from 5–7-day old YACQ84 transgenic mice using magnetic cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany). Whole brains were dissected and homogenized using the Neural Tissue Dissociation Kit P (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the separation of neurons from non-neuronal cells (astrocytes, oligodendrocytes, microglia, endothelial cells, and fibroblasts) using the Neuron Isolation Kit (Miltenyi Biotec, 130-115-389) following the kitś instructions ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533). Per 1 × 107 total cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspension was obtained by mechanical resuspension followed by filtration through a 40-μm-pore-size cell strainer and dead cel;ls were removed using Dead Cell Removal Kit (Miltenyi Biotec 130-090-101). Differentiating c-KIT+ neonate spermatogonia cells were magnetically labeled with CD117 (c-KIT+ ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+-cells were isolated by magnetic activated cell sorting (MACS) with positive selection using CD14 MicroBeads (130-050-201; Miltenyi Biotec B.V. & Co. KG) from freshly isolated SVF or PBMCs according to supplier’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... CD3/CD28-mediated activation of T cells was conducted by using 10 μl of T cell TransActTM (130-111-160; Miltenyi Biotec B.V. & Co. KG) according to manufacturer’s instructions (T cell activation medium) ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was enzymatically and mechanically dissociated into a single-cell suspension using the magnetic activated cell sorting (MACS) Neural Tissue Dissociation Kit (Miltenyi Biotec 130-093-231). Myelin was removed using the MACS Myelin Removal Beads (Miltenyi Biotec 130-096-733) ...
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bioRxiv - Immunology 2022Quote: ... single-cell suspensions from liver and BM were depleted of lineage-positive (Lin+) cells using a Direct Lineage Cell Depletion Kit (Miltenyi Biotec, 130-110-470) and stained for the following HSPCs ...
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bioRxiv - Microbiology 2021Quote: Total spleen T cells were purified by negative selection with the mouse naïve CD4+ T Cells Isolation Kit (Miltenyi Biotec, Cat# 130-104-453) and LS separation columns (Miltenyi Biotec ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2021Quote: ... The Lin− fraction of the CB cells and the AML cells were further enriched for CD34 with the human CD34 MicroBead Kit UltraPure (Miltenyi Biotec, 130-100-453), which contains microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype ...
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bioRxiv - Immunology 2021Quote: ... Open-repertoire T cells (7.5e106 cells per well of a 24 well plate) were stimulated with OKT3 (50 ng mL−1, Miltenyi Biotec, Cat 130-093-387) and rhIL-2 (300 IU mL−1 ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Microbiology 2022Quote: CD4+ T cells were isolated from PBMC population using the CD4+ isolation kit (CD4+ T cell Isolation kit II, Miltenyi Biotec, San Diego, CA). CD4+ T cells were first cultured for three days using stimulating conditions (RPMI supplemented with 10% donor autologous serum ...
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bioRxiv - Immunology 2022Quote: Splenic B cells from 24-week old MRL/lpr mice were isolated with a Pan B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). B cells were cultured with RPMI-1640 medium (Lonza ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and CD19+ B cells were primarily sorted from PBMCs of convalescents and vaccinees by CD4 and CD19 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany), respectively ...
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bioRxiv - Systems Biology 2022Quote: Naïve CD8+ T cells were isolated from the peripheral blood of two healthy donors using the Naive CD8+ T Cell Isolation kit (Miltenyi Biotec, 130-093-244) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Single-cell suspensions of CD4+ or CD8+ T-cells were prepared from spleens by negative selection using magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Naive 6-week-old IFN-αβR-/- mice (n = 4-9 ...
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bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... Primary CD4+ T lymphocytes were isolated from peripheral blood mononuclear cells and purified by negative selection using the CD4+ T cell isolation kit (Miltenyi Biotec, 130-096-533). Cells were phenotypically analyzed and activated using anti- CD3/antiCD28 coated plates (Ref BE0001-2 and Ref BE0248 ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions of splenocytes from individual mice were prepared and B cell enrichment was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). Plasmablast cell sorting was performed using a FACSAria (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... Untouched T cells were isolated from PBMCs by negative Magnetic-activated cell sorting (MACS) using the human pan T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... 10 µl of FcR blocking reagent was added per 107 of cells and 20 µl per 107 cells of Anti-LGR5 MicroBeads (Miltenyi Biotec, Inc., CA, USA) were added to the final cells suspension and incubated for 20 minutes in dark at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Digestion was stopped with ice-cold DMEMF12 and the supernatant was sequentially filtered through a 100 μm cell strainer (Falcon, 352360) and a 30 μm cell strainer (Miltenyi Biotec, 130-098-458). The fraction retained above the 30 μm filter ...
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bioRxiv - Bioengineering 2024Quote: ... The cell pellet was resuspended in 80 µL of FACS buffer and 20 µL of the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268). The cells were incubated for 15 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... CD14+ cells were sorted with the MACS system (Miltenyi Biotec), according to the manufacturer instructions and seeded at 5×105 cells/mL in RPMI-1640 media with L-glutamine (Lonza ...
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bioRxiv - Immunology 2022Quote: ... isolated using the mouse Lineage Cell Depletion Kit (Miltenyi Biotec). Lineage negative HPCs were transduced with either GFPNKG2D-S/L or RFP-Ly49A and cultured in a differentiation-conditioned RPMI medium as described (4).
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bioRxiv - Genetics 2019Quote: ... Cells were analyzed in a MACS Quant analyzer (Miltenyi Biotec) in 96-well format with the same established settings ...