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Citations for Miltenyi Biotec :
901 - 950 of 985 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Lineage negative cells (Lin-) were obtained by depleting lineage positive cells (Lin+) from the BM cells using MACS buffer (Miltenyi Biotec, #130-091-221), Lineage Cell Detection Cocktail-Biotin (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: Single cells from hamstring muscles were isolated using a skeletal muscle dissociation kit (Miltenyi Biotech) and GentleMACS Dissociator tissue homogenizer (Miltenyi Biotec, Macquarie Park, Australia) as previously described20 ...
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bioRxiv - Microbiology 2021Quote: ... Lungs were removed from animals and the right lobes of the lung were homogenized in 1mL of PBS in Miltentyi C tubes (Miltenyi Biotec 130-096-334) using the m_lung_02 program on a Miltenyi gentleMACS tissue dissociator ...
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bioRxiv - Cancer Biology 2021Quote: Control and constricted MDA-MB-231 cells were cocultured with human NK cells sorted from peripheral blood using the NK cell isolation Kit (Miltenyi Biotec 130-092-657) at the ratio of 1:20 ...
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bioRxiv - Immunology 2021Quote: B cells were purified from splenocytes by positive selection using magnetic beads-conjugated monoclonal anti-mouse B220 antibody (clone: RA3-6B2, Miltenyi Biotec, Bergisch Gladbach, Germany). Isolated B cells (5 × 105/mL ...
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bioRxiv - Immunology 2020Quote: The exosomes extracted from SVV-green fluorescence protein (GFP)-infected cells and normal cells were purified using an exosome purification kit (Miltenyi Biotec, Bergisch Gladbach, Germany), and the copy number of SVV was detected ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was assessed by Annexin V exposure and PI incorporation using the FITC-Annexin V/PI kit from Miltenyi Biotec (#130-092-052) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... T cells were enriched from spleen homogenate by magnetic-activated cell sorting (MACS) using Miltenyi Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Stefano Rivella) and CD34+ cells were isolated by positive selection using the human CD34 Microbead kit from Miltenyi Biotec (Catalog No.130-046-702) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... single-cell suspensions from liver and BM were depleted of lineage-positive (Lin+) cells using a Direct Lineage Cell Depletion Kit (Miltenyi Biotec, 130-110-470) and stained for the following HSPCs ...
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bioRxiv - Immunology 2022Quote: ... MCs were enriched from PBMCs with manual magnetic activated cell separation (MACS) using the Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, The Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Immunology 2022Quote: Primary NK cells were enriched from normal human peripheral blood mononuclear cells (PBMCs) (Zenbio Inc.) using NK cell isolation kit (Miltenyi Biotec, 130-092-657). The purity of isolated NK cells was confirmed by staining of APC conjugated anti-human CD56 antibody and PE conjugated anti-human CD16 antibody ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were purified from CD4+ T cells from pooled spleens and lymph nodes of Cd226−/− 2D2 TCR Tg mice by using mouse CD4 MicroBeads (Miltenyi Biotec, Bergisch-Gladbach, Germany), followed by flow cytometric sorting for CD25-CD44LoCD62LHiCD4+ cells ...
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bioRxiv - Bioengineering 2022Quote: The cell pellet was then incubated in a 4 °C fridge with rocking for 15 minutes in 100 µL of MACS buffer and 25 µL of lineage depletion microbeads from the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... ≈1 gram of muscle tissues was dissected from the hindlimb muscles of a humanely euthanized mouse and placed into a GentleMACS dissociation tube (Miltenyi Biotec, #130-096-334). 7 mL of DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... MSC were grown from the fragments retained in the cell strainers in serum-free StemMACS MSC Expansion Media XF (Miltenyi Biotec, Bergisch Gladbach, Germany) supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Developmental Biology 2021Quote: ... single cells were obtained as described for flow cytometry from femurs and tibias of 12-week-old male mice and stained with microbead labelled antibodies to CD45 (Miltenyi Biotec, 130-052-301), CD117 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: Naive CD4+ T cells from spleens of wild-type C57BL/6J and P2rx7-/- mice were sorted with MACS columns (Miltenyi Biotec, Bergisch Gladbach, Germany). For cytokine-skewed Th17 differentiation ...
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bioRxiv - Cancer Biology 2022Quote: ... Fully de-identified human CD34 cells enriched blood samples were obtained from Bone Marrow and Transplantation Laboratory at UCSF and were MACS sorted using CD34 MicroBead Kit (Miltenyi Biotec, 130-046-702) and incubated with CD3 antibody (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Genomics 2022Quote: 3ug of biotinylated RNA and 60pg of in vitro transcribed spike-in RNA (ERCC-00048) in 200ul water was mixed with 100ul of beads from the uMACS Streptavidin kit (Miltenyi Biotec 130-074-101) on a thermoblock at 24°C at 800 RPM for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... FITC/PE anti-human REAfinity™ TCR Vδ1 (REA173) and APC anti-human REAfinity™ TCR Vδ2 (REA771) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany); PE/Cy7 anti-mouse FasL (MFL3) ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Microbiology 2022Quote: CD4+ T cells were isolated from PBMC population using the CD4+ isolation kit (CD4+ T cell Isolation kit II, Miltenyi Biotec, San Diego, CA). CD4+ T cells were first cultured for three days using stimulating conditions (RPMI supplemented with 10% donor autologous serum ...
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bioRxiv - Immunology 2022Quote: Splenic B cells from 24-week old MRL/lpr mice were isolated with a Pan B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). B cells were cultured with RPMI-1640 medium (Lonza ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and CD19+ B cells were primarily sorted from PBMCs of convalescents and vaccinees by CD4 and CD19 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany), respectively ...
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bioRxiv - Systems Biology 2022Quote: Naïve CD8+ T cells were isolated from the peripheral blood of two healthy donors using the Naive CD8+ T Cell Isolation kit (Miltenyi Biotec, 130-093-244) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Tregs were isolated from fresh PBMCs by magnetic separation using the CD4+CD25+CD127dim/- human Treg isolation kit (Miltenyi Biotec, Cat: 130-094-775). Tregs were then used directly for analysis or assays.
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bioRxiv - Cancer Biology 2024Quote: ... T-ALL cells were isolated from the spleen and bone marrow of mice by negative selection with mouse CD45 microbeads (Miltenyi Biotec #130-052-301).
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bioRxiv - Cancer Biology 2023Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The remaining submucosal tissue was placed in a conical tube containing fresh RPMI-1640 supplemented with digestion enzymes from the human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) and chopped into small pieces using dissection scissors ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
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bioRxiv - Microbiology 2023Quote: ... CD14+ cells were isolated from the PBMCs using human CD14+ micro beads conjugated with anti-human CD14 mAbs (130-118-906, Miltenyi Biotec, Bergisch Gladbach, Germany) LC columns (130-042-401 ...
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bioRxiv - Systems Biology 2023Quote: For primary cardiac myofibroblast enriched cultures cells were first isolated from adult mice hearts using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) and gentleMACS homogenizer (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells (CD4+CD44-CD62L+) were isolated from mouse spleen by negative selection using the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Purified naïve T cells were activated with plate-bound anti-CD3ε (5 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Single-cell suspensions of CD4+ or CD8+ T-cells were prepared from spleens by negative selection using magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Naive 6-week-old IFN-αβR-/- mice (n = 4-9 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
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bioRxiv - Microbiology 2023Quote: ... Purity of the PMN suspension was analyzed by flow cytometry (CD3-PerCP-/CD66b-FITC+/CD14-PElow cells > 90%, antibodies from Miltenyi Biotec, Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2023Quote: To assess immune cells and endothelia from the lung left lobe of lung was digested using a GentleMACS lung digestion kit (Miltenyi Biotec #130-095-927). In brief ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Primary CD4+ T lymphocytes were isolated from peripheral blood mononuclear cells and purified by negative selection using the CD4+ T cell isolation kit (Miltenyi Biotec, 130-096-533). Cells were phenotypically analyzed and activated using anti- CD3/antiCD28 coated plates (Ref BE0001-2 and Ref BE0248 ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions of splenocytes from individual mice were prepared and B cell enrichment was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). Plasmablast cell sorting was performed using a FACSAria (BD Biosciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Developmental Biology 2023Quote: ... Prenatal human microglia were purified from primary brain tissue from mid-gestation (gestational week 18-23) samples using magnetic-activated cell sorting (MACS) kit with CD11b magnetic beads (Miltenyi Biotec, 130-049-601) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... dead cells were removed from the culture via a Miltenyi Biotec Dead Cell Removal Kit and MiniMACs Separator with accompanying columns (Miltenyi Biotec, Bergisch Gladbach, Germany). CD61+ Mks were then enriched from the live cells using CD61 MicroBeads and a MidiMACs Separator with accompanying columns (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... CD61+ Mks were then enriched from the live cells using CD61 MicroBeads and a MidiMACs Separator with accompanying columns (Miltenyi Biotec, Bergisch Gladbach, Germany). The resulting sample of live CD61+ Mks was resuspended in IMDM media supplemented with BIT 9500 ...