Labshake search
Citations for Miltenyi Biotec :
901 - 950 of 1023 citations for 3 Piperidinol 1 methyl 4 2 4 6 trimethoxyphenyl cis + since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... for an additional 15 min and subsequently subjected to positive selection of Gr-1+ cells on a MACS LS column according to the manufacturer’s instructions (Miltenyi Biotec).
-
bioRxiv - Neuroscience 2020Quote: ... The perfused brains were dissected and cut into small 1 mm3 pieces before homogenizing them in DPBS++ containing 0.4% DnaseI on the tissue dissociator (Miltenyi Biotec) at 37 °C for 35 mins ...
-
bioRxiv - Cell Biology 2021Quote: ... The high pluripotent stem cells form colonies were selected by Anti-Tra-1-60-PE and Anti-PE MicroBeads following the manufacturer’s instructions (MACS Miltenyi Biotec). The stable colonies were frozen down at -80°C ...
-
bioRxiv - Systems Biology 2020Quote: ... Cell suspensions (up to 1×108 cells/500 µL of Magnetic Bead Buffer) were applied onto MS columns (Miltenyi Biotec) in a magnetic field ...
-
bioRxiv - Cell Biology 2021Quote: ... at 37°C for 1 hr and passed through a 70 μm and a 30 μm cell strainer (Miltenyi Biotec). CD45+ cells were isolated using magnetic beads (Miltenyi Biotec 130-052-301 ...
-
bioRxiv - Bioengineering 2022Quote: ... for the cardiac marker Cardiac troponin T conjugated to APC (cTnT; 1:50; REA400; 130-120-543, Miltenyi Biotec, Germany), and cells were frozen in RPMI + B27C with 10 µM of RI + 10% FCS + 10% DMSO ...
-
bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
-
bioRxiv - Immunology 2021Quote: ... OT-1 cells were purified from OT-1 transgenic mice using negative selection with CD8a+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec).
-
bioRxiv - Immunology 2020Quote: Freshly collected human decidual tissues were trimmed into 1 mm3 piece by GentelMACS Dissociator (Miltenyi Biotec, 130-093-235, Germany) and digested twice for 30 minutes each at 37°C with 1.0 mg/ml type IV collagenase (Gibco ...
-
bioRxiv - Developmental Biology 2021Quote: ... The stroma was negatively selected against epithelium using a EPCAM antibody coated microbeads (1:10; Miltenyi Biotec #130-061-101) and a Miltenyi MS column.
-
bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...
-
bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
-
bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
-
bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
-
bioRxiv - Genetics 2023Quote: ... and the precipitated pellet was resuspended in 2.5 mL 1× Buffer and filtered with a 70 µM MACS SmartStrainer (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Molecular Biology 2023Quote: ... or tumor necrosis factor-alpha (1 ng/mL, TNF-α; catalog no. 130-094-022, Miltenyi Biotec, Bergisch Gladbach, Germany) in combination with LPS ...
-
bioRxiv - Microbiology 2024Quote: ... they were stained with 100 μl of PE-conjugated mouse anti-human IgA (1:40; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
-
bioRxiv - Microbiology 2024Quote: ... These cells were then subjected to staining with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... and stained with antibodies against the following murine and human surface markers: murine CD45 (1/50 mCD45-VioBlue; Miltenyi Biotec), human CD45 (1/50 hCD45-APCvio770 ...
-
bioRxiv - Microbiology 2024Quote: ... 50 μL of 0.2% Tween-20/1X PBS containing 1 μL of PE-Foxp3 (Miltenyi Biotec cat# 130-111-678) was added and cells were incubated for 30 min in the dark ...
-
bioRxiv - Genetics 2024Quote: ... 1 × 10^7 cells were processed for dead cell depletion using Dead Cell Removal Kit (Miltenyi Biotec # 130-090-101) as per the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: ... then washed with 3 mL washing buffer (PBS + 1% BSA + 0.2 U/μL RNase inhibitor) and filtered through 30 μm MARC SmartStrainer (Miltenyi Biotec) twice ...
-
bioRxiv - Cell Biology 2024Quote: ... Lineage depletion (B220, CD3, Gr-1, and Ter119) was performed prior to sorting using MACS magnetic microbead kits (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were washed three times and incubated in PBS containing DAPI (1 μg/mL) for 15 min before samples were processed using flow cytometry (MACSQuant, Miltenyi Biotec). At least 10000 events were analyzed per sample using FlowLogic software (Miltenyi Biotec).
-
bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... The second (positive) magnetic separation was then performed using astrocyte-specific anti-GLAST (ACSA-1) antibodies conjugated to the magnetic beads (Miltenyi Biotec). Anti-O4 selection using magnetic microbeads (Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
-
bioRxiv - Immunology 2020Quote: ... the pre-enriched pDC were labeled with pDC-specific CD304 (BDCA-4/Neuropilin-1) Diamond MicroBeads and isolated by positive selection over a MS MACS Column and magnetic field MACS Separator (Miltenyi Biotec). Purified pDC were then counted and tested for purity by FACS staining with mouse anti-human antibodies directed against lineage markers (VioBlue-CD3 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
-
bioRxiv - Plant Biology 2022Quote: ... The blots were then incubated at room temperature with the monoclonal α-GFP antibody (1:5000) (Miltenyi Biotec, 130-091-833), α-RFP antibody (1:5000 ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
-
bioRxiv - Molecular Biology 2020Quote: PBMC were obtained from healthy donors and were cultured in 48-well plates at a density of 1 × 106/ml in TexMACS (Miltenyi Biotec), 3% human serum (Seralab ...
-
bioRxiv - Microbiology 2021Quote: ... 2 mM EDTA) and stained with 1 μl of an APC-conjugated CD21 specific antibody (clone: HB5; 130-101-739, Miltenyi Biotec). The cells were incubated at 4 °C in the dark for 20 min and washed with and resuspended in 200 μl FACS staining buffer ...
-
bioRxiv - Immunology 2021Quote: ... sorted B cells were cultured with rh IL-21 as previously described and with Human CD40-Ligand Multimer Kit (1 µg/mL, Miltenyi Biotec), rh IL-6 (20 ...
-
bioRxiv - Immunology 2021Quote: ... The right lungs as well as the right hemispheres of the brains were homogenized in 1 ml PBS using a gentleMACS Octo Dissociator (Miltenyi Biotec) and viral RNA was isolated from 140 µl cleared homogenate or BAL fluid using QIAamp Viral RNA Mini Kit (Qiagen) ...
-
bioRxiv - Cancer Biology 2020Quote: ... B16F10 cell lines were transfected with pRP[CRISPR]-hCas9-U6>PDPN gRNA 1 plasmid (constructed and packaged by Vector-builder; vector ID: VB160517-1061kpr) before negative selection using MACS LD columns (Miltenyi Biotec), anti-mouse podoplanin-biotin antibody (50 µg/mL ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were added at a dilution of 1:100 in autoMACs Rinsing Solution (Cat. No. 130-091-222, Miltenyi Biotec) containing 0.5% BSA at 4 °C for 1 hour ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary neurons where derived from neural tissue following standard operational procedure from p0-1 mice brain using the Neural Tissue Dissociation Kit-Postnatal neurons (Cat. 130-094-802, Miltenyi Biotec). After removing the meninges ...
-
bioRxiv - Immunology 2020Quote: ... The numbers of cells that migrated to the lower well after 1 hr incubation were counted using a MACSQuant flow cytometer (Miltenyi Biotec). The percent migration was calculated by the numbers of neutrophils that migrated into the bottom chamber divided by the total number of neutrophils in the starting cell suspension and multiplying the results by 100 ...
-
bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
-
bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
-
bioRxiv - Cancer Biology 2022Quote: Cryopreserved bone marrow mononuclear cells were thawed and stained using standard procedures with the surface antibody CD34-PE (clone AC136, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tumor cells were transduced with a MOI of 10 for 24 h using Vectofusin-1 (10 μg/mL; #130-111-163, Miltenyi Biotec), with the following selection in Puromycin 1-2 μg/ml (#P8833 ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 1 hour on ice with magnetic beads coated with antibodies against Pdgfra (Miltenyi Biotec #130-101-547). Separation of Pdgfra-positive and negative populations was performed with LS column (Miltenyi Biotec #130-042-401 ...
-
bioRxiv - Immunology 2022Quote: ... OT-1 T cells were isolated from the spleens OT-1 mice using negative selection with CD8a+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... cells were resuspended in 96-well U-bottom plates and blocked prior to staining with 1 μg/mL anti–CD40 (clone HB14, Miltenyi Biotec) for 30 minutes at 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were resuspended with 1 mL “re-expression medium” containing NeuroBrew-21 (1:50 in MACS neuro Medium) (130-093-566 and 130-093-570, Miltenyi Biotec) and 200 mM L-glutamine (1:100 ...
-
bioRxiv - Microbiology 2022Quote: ... Purified CD4+ T cells were characterized following counter-staining with anti-CD4-APC antibody (1:200) using flow-cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
-
bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
-
bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in 1 ml BSA/PBS and applied together with 1 ml BSA/PBS onto the prepared LS columns (# 130-042-401, Miltenyi Biotec) placed into a QuadroMACS™ Separator (# 130-091-051 ...