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Citations for Miltenyi Biotec :
751 - 800 of 985 citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). UCB or BM CD34+ HSPC subsets were directly seeded by FACS in the corresponding plates and cultured for 10 (bulk cultures ...
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bioRxiv - Molecular Biology 2023Quote: ... primary B cells were isolated from Rif1FH/FH and C57BL/6 (Rif1WT/WT) mice spleens using anti-CD43 MicroBeads (Miltenyi Biotec) and expanded in complete RPMI containing 5 µg/ml LPS (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... For two donors the B cells were isolated using MACS cell sorting with anti-human CD19 microbeads and MS columns from Miltenyi Biotec, according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: NK cells were enriched from the spleens of C57BL/6 Kaede mice using NK Cell Isolation Kit (#130-115- 818, Miltenyi Biotec) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... NK cells were isolated from PBMCs by negative selection using the human NK cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). NK cells were cultured in Roswell Park Memorial Institute medium (RPMI ...
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bioRxiv - Immunology 2023Quote: ... Fatty tissue was removed from the tonsil as much as possible with scalpels and placed into a gentleMACS C-tube (130-096-334, Miltenyi Biotec) with 5ml RPMI containing 0.3mg/ml Liberase (000000005401020001 Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... CD34+ cells were purified from a portion of the cells using the CD34 Microbead Kit Ultrapure and LS columns (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: Resting B-cells were enriched from the spleen using the B-cell Isolation Kit (Miltenyi Biotec, Germany, Cat# 130-090-862). 1 mL 8 million/mL B-cells in HBSS buffer were sequentially incubated with 1 μM cholesterol strand (30 min ...
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bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine cells were removed from the dead-cell-depleted fraction using a mouse cell depletion kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD viability staining solution (Biolegend) ...
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bioRxiv - Immunology 2023Quote: B220+ B cells were isolated via negative selection from single-cell suspensions by using the mouse B Cell Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cell lysis was performed with ACK buffer (Thermo) before mouse cells were removed from the dissociated lungs using the mouse cell depletion kit (Miltenyi Biotec) yielding purified MeWo cells that achieved successful lung colonization ...
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bioRxiv - Immunology 2023Quote: ... Lineage-positive cells were depleted from the resulting cell suspensions using the mouse direct lineage cell depletion kit (cat. 130-110-470, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was used to lyse erythrocytes from bone marrow cell suspension followed by T cells depletion using mouse CD3ε MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and the appropriate programs were run according to the mouse tumor dissociation kit protocol and mouse lung dissociation kit protocol (both from Miltenyi Biotec) for tumors and lungs respectively ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...
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bioRxiv - Cancer Biology 2023Quote: Primary BM mononuclear cells isolated from MM patients or healthy donors were enriched in CD138+ PCs using magnetic sorting with the CD138 Microbead Kit (Miltenyi Biotec). Cells were plated in 48-well plates previously seeded with human BM-derived mesenchymal cells.
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells from the sample were purified by magnetic bead separation using the CD4+CD62L+ isolation kit (Miltenyi Biotec). Cells were plated in 96-well plates coated with anti-CD3 antibody (5μg/ml ...
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bioRxiv - Physiology 2023Quote: ... cerebellum of WT and KIKO mice was dissociated to single-cell suspension using adult brain dissociation kit from MACS Technology and using GentleMACS (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... spleens and tumors from groups of mice were harvested and tumors were processed using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... spleens and tumors from groups of mice were harvested and tumors were processed using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... a single cell suspension from the hippocampus and cortex was prepared using Neural Tissue Dissociation Kit (papain-based) (Miltenyi Biotec GmbH). After blocking with CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were obtained from the lymph nodes (LNs) and spleens of OT-1 mice using a negative selection kit (130-104-075, Miltenyi Biotec). For CTL differentiation ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated from healthy donors via magnetic bead labeling (CD8+ T cell isolation kit, Miltenyi Biotec, Germany). U937 (ATCC ...
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bioRxiv - Immunology 2023Quote: CD4 T cells were isolated from the spleens of donor mice and purified using the mouse CD4 T Cell Isolation Kit (Miltenyi Biotec). The purity and cell count of donor CD4 T cells were assessed using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated from PBMCs through two rounds of magnetic enrichment using the human pan B cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cytotoxic T cells and T helper cells were isolated from the PBMCs by positive selection with CD8- and CD4-specific MicroBeads (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2023Quote: ... naive CD4+ T cells (CD4+CD25−CD62L+) were sorted from the spleen of WT C57Bl/6 mice using a commercially available kit (Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2023Quote: ... and the circulating CD45-PE+ cells were removed from the single cell suspension using magnetic sorting with anti-PE microbeads (Miltenyi Biotec) according to manufacturer’s recommendations (S1A Figure).
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bioRxiv - Neuroscience 2024Quote: ... the resulting pellet was resuspended in 100 μl of microbeads from the dead cell removal kit (Miltenyi Biotec, 130-090-101) and processed according to the commercial protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... CD8+T cells in single-cell suspensions were purified from tumors using a magnetic cell sorting column purification system (CD8+ T Cell Isolation Kit, Miltenyi Biotec). The conditioned medium (CM ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... CD4+ T cells were selected from resultant suspensions by labeling with bead conjugated anti-CD4 for enrichment by autoMACs (Miltenyi Biotec). Studies were approved by the Institutional Review Board at Baylor College of Medicine and informed consent was obtained from all patients.
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bioRxiv - Immunology 2024Quote: ... Human fetal thymus samples were HPCs were obtained from Ficoll-Hypaque–purified CB samples using the CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec), as described (38) ...
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bioRxiv - Immunology 2024Quote: ... CD4 and CD8 T cells isolated from spleen and lymph nodes were purified by incubating with anti-CD4 and CD8 microbeads (Miltenyi Biotec) and passing through LS magnetic columns following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated using Ficoll-Hypaque density centrifugation and pDCs were positively selected from PBMCs using BDCA-4-magnetic beads (MACS Miltenyi Biotec), as previously described (Decembre et al. ...
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bioRxiv - Bioengineering 2024Quote: ... liver tissues from the ALF model were dissociated using the gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427). Subsequently ...
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bioRxiv - Physiology 2024Quote: Primary human monocytes were isolated from healthy human blood samples using CD14-magnetic beads (cat. no.: 130-050-201, Miltenyi Biotec). In brief ...
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bioRxiv - Immunology 2024Quote: CD14-positive monocytes were isolated from peripheral blood mononuclear cells (PBMCs) with CD14 microbeads and MACS LS column system (Miltenyi Biotec). PBMCs were obtained from the buffy coats of twelve healthy blood donors (eight males and four females ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured overnight at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the tumor was carefully dissected from the affected hemisphere and processed using the Adult Brain Dissociation Kit (130-107-677, Miltenyi Biotec) for digestion ...
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bioRxiv - Neuroscience 2024Quote: Cortices were dissected from P1 and P14 brains and dissociated into single-cell suspension using Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628 for P1 and 130-094-802 for P14) ...
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bioRxiv - Immunology 2024Quote: ... was used to lyse red blood cells before T cell isolation using the naive CD4+ T Cell Isolation Kit from Miltenyi Biotec or the MojoSort™ Mouse CD4 or CD8 Naïve T Cell Isolation Kits from BioLegend ...
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bioRxiv - Immunology 2024Quote: ... CD8 T cells from autologous blood and tonsils from 4 HLA-A*02:01 donors were enriched using negative selection magnetic beads (CD4, CD19 depletion, Miltenyi Biotec). Roughly 1.5 e6 enriched CD8s were stained with Fc block for 10 minutes on ice ...
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bioRxiv - Cell Biology 2024Quote: ... hFlt3-Ligand 100ng/mL, 130-096-479; hIL-3 60ng/mL, 130-095-069; TPO 10nM, 130-094-013, all from Miltenyi Biotec) and 1% PS during 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... day 27 cultures were first enriched for motor neurons by using antibodies against PSA-NCAM and L1CAM (both from Miltenyi Biotec) [39] ...
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bioRxiv - Immunology 2024Quote: ... Murine T cells were isolated from C57Bl6/J (WT or IFNγ-/-) splenocytes with CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... total CD4+ T cells or total CD8a+ T cells were isolated from WT or Aim2−/−mice using a magnetic cellular isolation kit (CD4+, Miltenyi Biotec, 130-104-454 ...
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bioRxiv - Immunology 2024Quote: ... Tregs (CD4+ hCD2+ CD25+ DAPI-) were sorted from the spleen and LN (after B cell depletion with MACS CD19 microbeads, Miltenyi Biotec) of CD45.2 Foxp3-hCD2 WT and PI3KδD910A mice ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec). Monocytes were cultured at 37°C ...