Labshake search
Citations for Miltenyi Biotec :
701 - 750 of 765 citations for Recombinant Human BTN1A1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: Primary human CD4-positive T cells were isolated from enriched peripheral blood mononuclear cell (PBMC) fractions using the human CD4+ T cell isolation kit from Miltenyi Biotec (Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Mononucleated cells from PB or BM were then enriched for CD34+ cells on the AutoMacs system using human CD34 MicroBead kit (Miltenyi Biotec, #130-046-702) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533). Per 1 × 107 total cells ...
-
bioRxiv - Cancer Biology 2020Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: Control and constricted MDA-MB-231 cells were cocultured with human NK cells sorted from peripheral blood using the NK cell isolation Kit (Miltenyi Biotec 130-092-657) at the ratio of 1:20 ...
-
bioRxiv - Immunology 2022Quote: ... Alveolar-like MDMs were generated by differentiating CD4+ monocytes on tissue culture plates into macrophages in the presence of 50 ng/ml of human M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days ...
-
bioRxiv - Developmental Biology 2022Quote: ... Stefano Rivella) and CD34+ cells were isolated by positive selection using the human CD34 Microbead kit from Miltenyi Biotec (Catalog No.130-046-702) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Primary NK cells were enriched from normal human peripheral blood mononuclear cells (PBMCs) (Zenbio Inc.) using NK cell isolation kit (Miltenyi Biotec, 130-092-657). The purity of isolated NK cells was confirmed by staining of APC conjugated anti-human CD56 antibody and PE conjugated anti-human CD16 antibody ...
-
bioRxiv - Cancer Biology 2019Quote: Half of each tumor was dissociated using the Human Tumor Dissociation Kit and Gentle MACS Tissue Dissociator with Heaters (Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s instructions prior to enumeration of live cells using trypan blue.
-
bioRxiv - Bioengineering 2021Quote: ... The Lin− fraction of the CB cells and the AML cells were further enriched for CD34 with the human CD34 MicroBead Kit UltraPure (Miltenyi Biotec, 130-100-453), which contains microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (isotype ...
-
bioRxiv - Cancer Biology 2021Quote: ... MM cells were pelleted and resuspended in flow cytometry buffer (1× PBS with 1% FBS) then incubated with human FcR blocking reagent (Miltenyi Biotec, # 130-059-901) before staining with diverse fluorescent dye-conjugated monoclonal antibodies ...
-
bioRxiv - Cell Biology 2022Quote: Purified milk and serum EVs were subjected to multiplex bead-based analysis by flow cytometry using the human MACSPlex Exosome Kit (Miltenyi Biotec, 130-108-813). EV samples were processed overnight in the MACSPlex Filter Plate as described in the manufacturer’s instructions and in a previously published paper on a systematic evaluation of the assay19 ...
-
bioRxiv - Immunology 2022Quote: ... Human monocytes were differentiated into a macrophage-like phenotype (MDM, monocyte derived macrophages) using M-CSF (50 ng/mL, Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days at 37ºC and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM ...
-
bioRxiv - Immunology 2024Quote: ... Tregs were isolated from fresh PBMCs by magnetic separation using the CD4+CD25+CD127dim/- human Treg isolation kit (Miltenyi Biotec, Cat: 130-094-775). Tregs were then used directly for analysis or assays.
-
bioRxiv - Cancer Biology 2023Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The remaining submucosal tissue was placed in a conical tube containing fresh RPMI-1640 supplemented with digestion enzymes from the human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) and chopped into small pieces using dissection scissors ...
-
bioRxiv - Cell Biology 2024Quote: The tissue pieces remaining after enzymatic treatment and filtration of the fresh sample were processed using a human tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions to obtain a single-cell suspension ...
-
bioRxiv - Synthetic Biology 2023Quote: Functional viral titres of retroviral supernatant were calculated using frozen supernatant on primary human T cells activated with TransAct (Miltenyi Biotec; 130-111-160), 10 ng/ml IL-7 (Miltenyi Biotec ...
-
bioRxiv - Immunology 2023Quote: ... Untouched T cells were isolated from PBMCs by negative Magnetic-activated cell sorting (MACS) using the human pan T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... and pDCs were purified using a negative selection MACS beads-based assay (Plasmacytoid Dendritic Cell Isolation Kit II human, Miltenyi Biotec, Bergisch Gladbach, GER), following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mice were sacrificed at predetermined timepoints and human CD45+ cells were isolated from bone marrow and spleen using the mouse cell depletion kit (Miltenyi Biotec, #130-104-694) to achieve >90% purity of human cells.
-
bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
-
bioRxiv - Microbiology 2021Quote: ... supernatants were collected and stored at −80 °C until analysis for cytokine secretion using the human MACSplex cytokine 12-kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to manufacturer’s protocol.
-
bioRxiv - Immunology 2020Quote: ... We magnetically isolated all CD4+ T cells from each sample using a human CD4+ T cell negative isolation kit (Miltenyi biotec, Cat #130-096-533) per manufacturer instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... Media supplements tested were FGF-2 at a concentration of 10 ng/ml (Human FGF-2 IS premium grade, MACS Miltenyi Biotec, Germany, 130-104-922), EGF at a concentration of 10 ng/ml (Recombinant Human EGF ...
-
bioRxiv - Biophysics 2024Quote: Primary human NK cells from healthy donors were isolated from blood samples provided by the EFS (Marseille, France) by negative selection using the MACSxpress Whole Blood human NK cell isolation kit (Miltenyi Biotec cat. 130-098-185), according to the manufacturer protocol ...
-
bioRxiv - Immunology 2024Quote: Primary CD4+ T cells were enriched from healthy donor cryopreserved PBMCs by negative selection (CD4+ T Cell Isolation Kit human, Miltenyi Biotec, cat. 130-096-533) and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3 ...
-
bioRxiv - Plant Biology 2020Quote: ... Eluted proteins were analysed by western blot using HRP-conjugated anti-myc antibody (130-092-113; Miltenyi Biotec), or an anti-HA antibody (3F10 ...
-
bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...
-
bioRxiv - Plant Biology 2021Quote: ... Proteins fused to mCITRINE were immunoprecipitated using the pull-down kit Miltenyi μMacs anti-GFP (Miltenyi Biotec®). Crude extracts were put in 1XSDG supplemented with bromophenol blue and denaturated at 95°C for 5 min while IP samples were directly ready for migration on polyacrylamide gels.
-
bioRxiv - Immunology 2020Quote: ... The splenocytes were incubated with the spike protein peptide pool at 1.5 μg/ml/peptide (Miltenyi Biotec, Germany) at 37 °C for 16-18 hours ...
-
bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
-
bioRxiv - Plant Biology 2020Quote: ... Co-immunoprecipitation was conducted using the μMACS™ Epitope Tag Protein Isolation Kits according to manufacturer’s instructions (Miltenyi Biotec), and purified on magnetic μ Column (Miltenyi Biotec) ...
-
bioRxiv - Plant Biology 2019Quote: ... RPS5A::mCherry-PDK1.2 were used for Co-IP assays using a µMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec), with RPS5A::mCherry-PDK1.1 and RPS5A::mCherry-PDK1.2 transgenic lines as controls ...
-
bioRxiv - Immunology 2022Quote: ... the complete sequence of B.1.1.7 D614G Spike mutant (alpha) (JPT Peptide Technologies) or wildtype (WT) S-protein (Miltenyi Biotec) or left untreated as a control for 16 h ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec #130-091-833), anti-plant actin mouse monoclonal antibody (3T3)-HRP (Abbkine # A01050HRP) ...
-
bioRxiv - Molecular Biology 2023Quote: All datasets contained RNA and protein images and were analyzed in the MACS iQ View analysis software (Miltenyi Biotec). Every field of view was assessed across two exposure times during image preprocessing ...
-
bioRxiv - Genomics 2024Quote: ... the cytotrophoblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin (APC)-conjugated anti-human leukocyte antigen (HLA)-ABC antibody (APC-anti-HLA-ABC, Miltenyi Biotec, #130-101-467, lot #5191002371). The Hofbauer/fibroblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin-conjugated anti-epidermal growth factor (EGFR ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell pellets were resuspended in 100 µl cold D-PBS and stained with fluorochrome-conjugated antibodies recognizing human CD11b and GFAP (Miltenyi Biotec, 130-113-810 and 130-123-846) as described above ...
-
bioRxiv - Developmental Biology 2021Quote: ... and seeded at a density of 19,000 cells/cm2 on Matrigel in mTeSR1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec). Upon reaching 95% confluence ...
-
bioRxiv - Bioengineering 2022Quote: ... or the PepTivator SARS-CoV-2 Prot_N peptide mix (covering the complete sequence of the SARS-CoV-2 N protein; both Miltenyi Biotec). As controls splenocytes were either left unstimulated or activated polyclonally with 12.5 µg/mL phytohemagglutinin (PHA) ...
-
bioRxiv - Plant Biology 2022Quote: ... The corresponding supernatant was used for immunoprecipitation assay with anti-FLAG micro beads according to the manufactureŕs instructions (μMACS Epitope Tag Protein Isolation Kit (MACS Miltenyi Biotec)) ...
-
bioRxiv - Bioengineering 2023Quote: ... the yeast cells were washed once with PBSB and incubated with 750 μL of Protein A microbeads (Miltenyi Biotec, 130071001) per 109 cells ...
-
bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
-
bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
-
bioRxiv - Cell Biology 2019Quote: ... The MicroBeads/cell extracts mixture was then applied to the μColumn followed by gentle wash with the lysis buffer used above for protein extraction (Miltenyi Biotec). Pre-heated (95°C ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
-
bioRxiv - Immunology 2023Quote: ... Duplicate cultures were stimulated with or without a combined preparation of Peptivator Peptide Pools of the complete Spike protein and predicted immunodominant sequences (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Cell Biology 2024Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...