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Citations for Miltenyi Biotec :
701 - 750 of 2531 citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...
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bioRxiv - Biochemistry 2021Quote: ... with an incubation time of 3h at room temperature and using secondary labels 0.6 µL anti-c-myc-FITC (Miltenyi Biotec) and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82) ...
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bioRxiv - Neuroscience 2022Quote: ... Up to 1.25 grams of cortical tissue were placed in each gentleMACS™ C Tube (Miltenyi Biotec, # 130-093-237) which contained 5 mL of 0.3 mg/mL papain (Worthington ...
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bioRxiv - Neuroscience 2022Quote: ... The gentleMACS™ C Tubes were placed on the gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec, # 130-096-427) with heating cuffs attached and underwent the gentleMACS Program 37C_ABDK_01 protocol ...
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bioRxiv - Immunology 2020Quote: ... for 30 minutes at 37°C and dissociated through a 30-mm pore cell strainer (Miltenyi Biotec, Bergisch Gladbach, Germany). For chemokine binding assays ...
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bioRxiv - Immunology 2021Quote: ... the samples were gently incubated with a proprietary enzymatic solution in C-tubes (Miltenyi Biotec, Cat. No.: 130-096-334) at 37°C for at least 30 minutes using a rotator ...
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bioRxiv - Immunology 2023Quote: ... then incubated for 15 min at 4 °C in labeling buffer containing streptavidin-conjugated and anti-FITC MicroBeads (Miltenyi Biotec). Cells were negatively selected using MACS LD columns per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and NK1.1(PK136) PE-conjugated antibody for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec) as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... Dissociated kidneys were incubated for 30 minutes at 37 °C with continuous rotation using the MACSmix Tube Rotator (Miltenyi Biotec). GentleMACS C tubes were then placed in the gentleMACS Dissociator and Multi_E_02 program was run ...
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bioRxiv - Immunology 2023Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... In short small pieces of full-thickness specimens were placed in a gentleMACS C-tube (130-093-237, Miltenyi Biotec) in digestion solution and dissociated in the gentleMACS Octo Dissociator (130-095-937 ...
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bioRxiv - Immunology 2023Quote: Processing of peripheral and cryopreservation was completed as previously described.8 Fresh tumor tissue was diced with a sterile scalpel and dissociated in 0.1% collagenase in RPMI 1640 for 60 minutes at 37⁰C using the gentleMACS OctoDissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Neuroscience 2024Quote: ... C-tubes tightly closed and attached upside down onto the sleeve of the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec.). The appropriate program 37C_ABDK_02 was run for 30 minutes ...
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bioRxiv - Immunology 2023Quote: Human CD4+ T cells were isolated by employing a CD4+ T cells isolation kit (Miltenyi Biotec kit) following the provider’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... the leukocyte fraction was enriched for CD8+ T cells using anti-mouse CD8a microbeads (Miltenyi Biotec) and autoMACS equipment (Miltenyi Biotec ...
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bioRxiv - Genetics 2020Quote: ... CD43 positive cells were first depleted using anti-mouse CD43 (Ly-48) MicroBeads (MACS Miltenyi Biotec). Donor derived cells were then isolated by FACS sorting using fluorochrome-labelled antibodies CD45.2-FITC ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Developmental Biology 2021Quote: ... Negative cell populations will be incubated with anti-Mouse IgG beads (Miltenyi Biotec, 130-048-402), and then MS coulumn (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Primary mouse CD4+ T cells were cultured in complete TexMACS Medium (Miltenyi Biotec, #130-097-196) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with anti-mouse CD45 MicroBeads and subjected to negative depletion (Miltenyi Biotec Inc). Cells were either used fresh for ex vivo experiments or preserved in CryoStor® CS10 freezing media (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... CD34+ cells were enriched via immunoselection using anti-mouse IgM microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) and the anti-CD34 hybridoma clone 12.8 (a murine IgM anti-human CD34 cross-reactive with RM CD34) ...
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were isolated using positive selection with mouse CD4/CD8 (TIL) MicroBeads (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 20 min on ice with anti-mouse ACSA2-APC (Miltenyi Biotec, USA). Astrocytes were sorted based on ACSA2 positive ...
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bioRxiv - Neuroscience 2024Quote: Purified single-cells suspensions were stained using antibodies against mouse CD45 (Miltenyi Biotec; 130-117-498), SCA1 (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... A 1:1 mixture of CD3-depleted (Miltenyi Biotec) Tollip-/- (CD45.2+ ...
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bioRxiv - Cell Biology 2023Quote: ... insulin-like growth factor 1 (IGF-1, Miltenyi Biotec), FGF-2 and LDN193189 ...
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bioRxiv - Cancer Biology 2021Quote: Freshly resected tumor or adjacent normal lung tissue was thoroughly chopped with surgical scissors and transferred to GentleMACS C Tubes (Miltenyi Biotec) containing 20 uL/mL Liberase TL (5 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stored at 4°C in dark until the next morning for data acquisition using a MacQuant10 (Miltenyi Biotec, USA). Data was analyzed using FlowJo 10 (BD Biosciences ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Neuroscience 2022Quote: ... the contents of the gentleMACS™ C Tube were strained through a 70 μm cell strainer (Miltenyi Biotec, # 130-110-916) placed on top of a 15 mL conical centrifuge tube ...
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bioRxiv - Genetics 2019Quote: ... The flow-through was pelleted at 1250 rpm in a swinging bucket centrifuge set to 6°C then resuspended in 3 ml cold AutoMACs Running Buffer (AMB, Miltenyi Biotec). After gentle resuspension ...
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bioRxiv - Cell Biology 2020Quote: ... Mechanical and enzymatic muscle digestion was then performed at 37°C on the gentleMACS with heaters (cat nb. 130-096-427, Miltenyi Biotec) for 60 min using a skeletal muscle digestion program (37C_mr_SMDK1).
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... we dissociated tumor cells from a fresh primary HB tumor using an enzyme mix in a gentleMACS C Tube (Miltenyi Biotec). Cells were spun ...
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bioRxiv - Immunology 2021Quote: ... and transplanted with syngeneic BALB/c bone marrow cells (5 × 106) after T-cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). For retransfer experiments ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with the following antibodies for 30 minutes at 4°C: CD3 VioGreen (clone BW264/56, Miltenyi Biotec), CD14 V500 (clone M5E2 ...
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bioRxiv - Genetics 2022Quote: ... Human tonsil tissue was processed by mincing with scissors followed by transfer of up to 4g of tissue to a gentleMACS C tube (Miltenyi Biotec) containing 8 mL of phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... After another incubation of 30 minutes at 37°C with 5% CO2 the samples are analysed by flow cytometry (MACS Quant, Miltenyi Biotec) and FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
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bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
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bioRxiv - Immunology 2022Quote: All cell culture was performed in a humidified incubator with 5% CO2 at 37°C using cRPMI (see above for ingredients) supplemented with 150ng/mL αCD40 (clone FGK-45; Miltenyi Biotec) and 25ng/mL recombinant murine IL-4 (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumours were collected into cold PBS then manually cut into small pieces and digested in GentleMACS C dissociation tubes using the GentleMACS tissue dissociator (Miltenyi Biotec) with the manufacture’s enzymes (liver dissociation kit ...
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bioRxiv - Physiology 2023Quote: ... CD11b/c positive cells (monocyte/macrophage) were sorted using magnetic-activated cell sorting (MACS) separation columns (Miltenyi Biotec, 130-105-634) and immediately lysed with TRIzol (Fischer Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Immunology 2023Quote: ... Fatty tissue was removed from the tonsil as much as possible with scalpels and placed into a gentleMACS C-tube (130-096-334, Miltenyi Biotec) with 5ml RPMI containing 0.3mg/ml Liberase (000000005401020001 Sigma ...
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bioRxiv - Neuroscience 2023Quote: The hippocampi from both hemispheres of Cx3cr1-NuTRAP mice were cut into four pieces and added to the pre-cooled gentleMACS C-tube (#130-096-334, Miltenyi Biotec) pre-filled with 2 mL ice-cold Nuclei Extraction Buffer (#130-128-024 ...
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bioRxiv - Immunology 2023Quote: ... Lungs were then incubated at 37 C and dissociated using a GentleMACS Octo Dissociator with Heaters (Miltenyi Biotec #130-095-937). Cells were then filtered through a 70-micron mesh filter ...
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bioRxiv - Immunology 2023Quote: ... perfused murine lungs were placed in a gentle MACS C tube and mechanically homogenized in 5 ml of PBS using a gentle MACS Octo Dissociator (Miltenyi Biotec). Lung cell suspensions were lysed of RBCs ...