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Citations for Miltenyi Biotec :
701 - 750 of 1240 citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Samples were then incubated for 15 min with CD11b antibody conjugated magnetic beads (Miltenyi Biotec) before passage through a magnetic bead column (Quadro MACS Separator and LS columns ...
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bioRxiv - Cancer Biology 2022Quote: ... The antibodies chosen for the staining were: anti-GFAP APC (130-124-040, Miltenyi Biotec) for astrocytes ...
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bioRxiv - Cell Biology 2019Quote: ... Magnetic columns and antibody-conjugated magnetic beads were purchased from Miltenyi Biotec (San Diego, CA). Liberase TL Research Grade enzyme mix and DNase I used in lung digestion were purchased from Roche Applied Science (Pleasanton ...
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bioRxiv - Immunology 2021Quote: ... CD14 cells were purified by anti-CD14 monoclonal antibody (mAb)-conjugated magnetic microbeads (Miltenyi Biotec) and then cultured for 6 days in RPMI 1640 medium (Life Technologies Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with cardiac troponin T APC-conjugated antibody (Miltenyi Biotec, 130-120-403) or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with micro-magnetic beads conjugated Thy1.2 antibody (Miltenyi Biotec, 130-121-278) for 20 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... incubated with magnetic microbeads coupled to GFP antibodies (µMACS™ GFP Isolation Kit, Miltenyi Biotec) and complexes were eluted in 100µl of pre-warmed elution buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... CH12-F3 cells were stimulated with agonist anti-CD40 antibody (0.5μg/ml; Miltenyi Biotec; FGK45.5), mouse IL-4 (5 ng/μl ...
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bioRxiv - Immunology 2024Quote: ... followed by staining with the following antibodies: anti-CD3 epsilon-VioBlue (Clone 17A2, Miltenyi Biotec), anti-TCR β-Alexa Fluor 488 (Clone H57-597 ...
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bioRxiv - Neuroscience 2023Quote: ... TRA-1-60+ iPSCs were isolated from cultures using anti-TRA-1–60 MicroBeads (Miltenyi Biotec) and LS columns (Milteny Biotec) ...
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bioRxiv - Immunology 2022Quote: ... Then 1.5x 105 cells/cm2 in 24-well dishes were seeded and mouse receptor activator of nuclear factor kb ligand (RANKL, Miltenyi Biotec, Germany) was added at the final concentration of 35 ng/ml to initiate osteoclasts (OC ...
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bioRxiv - Immunology 2019Quote: ... Bone-marrow (BM)-PMNs were isolated from the bone marrow using magnetic separation (mouse Neutrophil isolation kit, Miltenyi Biotec, 130-097-658) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... bone marrow of RBD-his tag protein immunized mice were isolated and purified with mouse CD138 MicroBeads (Miltenyi Biotec, 130-098-257) following standard protocol provided by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 480 μL staining buffer before adding 120 μL anti-mouse IgG2a/b MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for an incubation period of 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... via dounce homogenization and 30% Percoll gradient or enzymatically digested using mouse tumor dissociation kit and gentleMACS™ Octo Dissociator (Miltenyi Biotec). Spleens and lymph nodes were either digested with Collagenase IV/DNAse or not digested before mechanical disruption ...
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bioRxiv - Cell Biology 2023Quote: ... liver tissues were cut into small pieces and dissociated on the gentleMACS Dissociator with the Mouse Liver Dissociation Kit (Miltenyi Biotec, 130105807). Leukocytes were enriched by centrifugation with 40% Percoll ...
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bioRxiv - Cell Biology 2023Quote: ... cut longitudinally into roughly 2-mm-long pieces and were isolated using the Liver Dissociation Kit (mouse) and GentleMACS Dissociator (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Mouse scars and human stimulated skin and scar samples were digested in Miltenyi Whole Skin dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) for 2.5h according to the manufacturer’s protocol and processed on a GentleMACS OctoDissociator (Miltenyi) ...
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bioRxiv - Cell Biology 2023Quote: ... we purified some of the myoblast cultures using the mouse Satellite Cell Isolation Kit (130-104-268, Miltenyi Biotec, Bergisch-Gladbach, Germany) when necessary due to higher fractions of fibroblasts (e.g. ...
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bioRxiv - Immunology 2023Quote: ... Tregs were purified from the mouse lymphocytes using a CD4+CD25+ regulatory T cell isolation kit (Miltenyi Biotec, cat. # 130-091-041). The purity of the isolated cells was checked by flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Mouse CD4+ T cells were purified by negative selection using a CD4+ T cell isolation kit (Miltenyi Biotec, cat. # 130-104-454). For CD8 depletion ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from Day15 B16.F10 melanomas of C57BL/6 mice or α-SMAcreAtg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). 5 x 104 cells CAFs were seeded on poly-L-Lysine coated glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: ... melanomas of α-SMAcreAtg5fl/fl and Atg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). RNA was extracted with the Macherey-Nagel NucleoSpin® RNA kit ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...
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bioRxiv - Genomics 2020Quote: ... 1:100 B27 supplement (Miltenyi Biotec) and 1:40,000 Insulin (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... GLAST (clone ACSA-1, Miltenyi Biotec). Cells were incubated with antibodies for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130-093-387 and 130-093-375) and IL-2 (100 U/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130- 093-387 and 130-093-375), and IL-2 (100 U/mL ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then trypsinized and incubated with anti-CD146 antibody coated microbeads (Miltenyi Biotec Inc.) for 15 mins at 4°C to obtain the CD140b+CD146+ cells for subsequent experiments ...
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bioRxiv - Neuroscience 2020Quote: ... where Magnetic isolation (MACS) was performed with anti-NG2 antibody conjugated with magnetic beads (Miltenyi Biotec). For the P9 time-point ...
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bioRxiv - Microbiology 2021Quote: ... The antibodies used for FACS analysis were APC-conjugated anti-CD163 (clone REA812, MACS Miltenyi Biotec), PE-conjugated mouse anti-HLA-DR (clone LN3 ...
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bioRxiv - Immunology 2022Quote: Separation was performed using anti-CD3 antibodies conjugated to ferromagnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) and directed through a cell separation column containing a magnetic field (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were stained with the anti-CD326 (EpCAM) magnetic microbeads antibody (Miltenyi Biotec, 130-105-958) for 40 min on ice ...
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bioRxiv - Cancer Biology 2019Quote: ... we also used CD3-PE to specifically identify lymphocytes (human antibody, clone BW264/56, Miltenyi Biotec). For all the samples ...
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bioRxiv - Cancer Biology 2020Quote: ... Merkel cells were stained with anti-PSA-NCAM-PE antibody (Miltenyi Biotec, Cat. 130-117-394) or with mouse IgM-PE isotype control (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2023Quote: ... Conjugated primary antibodies used was anti-cardiac troponin 2 (TNNT2) PE (Miltenyi Biotec – 130-120-405). Viability marker Zombie Aqua (Biolegend – 423102 ...
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bioRxiv - Bioengineering 2023Quote: ... streptavidin magnetic beads were first conjugated with the desired biotinylated antibody (anti-CD63, biotin; Miltenyi Biotec) and allowed to incubate with the sample stirring overnight ...
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bioRxiv - Cell Biology 2020Quote: Neonatal mouse cardiac myocytes (NMCMs) were isolated using the MACS cell separation kit (Miltenyi Biotec: Catalog #130-100-825 and #130-098-373). Briefly ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... T cells were magnetically separated from other splenocytes using the Mouse Pan-T Cell Isolation Kit II (Miltenyi Biotec, Cat# 130-095-130), labeled with CellTrace™ CFSE (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... excised tumors (200 mg) were dissociated in RPMI media containing enzyme cocktail mix from the mouse tumor dissociation kit (Miltenyi Biotec, Auburn, CA) and placed into gentleMACS dissociators per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
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bioRxiv - Cancer Biology 2023Quote: MDSCs were isolated from the spleens of 4T1 tumor-bearing mice using a Mouse MDSC Isolation Kit (Miltenyi Biotec, Cat# 130-094-538) and plated in RPMI1640 supplemented with 10% FBS and antibiotics ...
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bioRxiv - Neuroscience 2024Quote: ... Lineage-negative HSCs were isolated from the bone marrow of donor mice via immunomagnetic separation using a mouse lineage cell depletion kit (Miltenyi Biotec, Auburn, CA). Lineage-negative HSCs were transduced overnight at a viral multiplicity of infection (MOI ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were stained with the following antibodies: anti-Nanog-APC (Cat#130-104-480, Miltenyi Biotec), anti-Gata6-PE (Cat#26452 ...
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bioRxiv - Cancer Biology 2019Quote: ... The live cell fraction was then incubated with CD45 magnetically-labeled antibody (Miltenyi Biotec, 130-052-301) and passed through a LS magnetic column (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: ... CD56+ cell fractions were collected using antibody-coated magnetic beads (Dynabeads,Thermo Fisher Scientific; CD56, Miltenyi Biotec). Antibody purified fractions were then extracted for DNA and RNA using the AllPrep DNA/RNA/miRNA Universal Kit according to the manufacturer’s instructions (QIAGEN).
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bioRxiv - Cancer Biology 2020Quote: ... c-Kit+ cells were enriched using magnetic beads conjugated with an anti-c-Kit antibody (Miltenyi Biotec), transduced with a recombinant retrovirus by spinoculation ...
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bioRxiv - Cell Biology 2021Quote: ... washed and incubated with an anti-IgM FITC-conjugated secondary antibody (Miltenyi Biotec Cat#: 130-095-906) (1:500 dilution in blocking buffer) ...
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bioRxiv - Immunology 2022Quote: Monocytes were isolated from freshly collected PBMCs using CD14 antibody-conjugated microbeads and magnetic columns (Miltenyi Biotec) according to the manufacturer’s protocol ...