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Citations for Miltenyi Biotec :
651 - 700 of 984 citations for 4 3 2 Chloroethyl 3 nitrosoureido tetrahydro 2H thiopyran 1 1 dioxide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 50 μL of 0.2% Tween-20/1X PBS containing 1 μL of PE-Foxp3 (Miltenyi Biotec cat# 130-111-678) was added and cells were incubated for 30 min in the dark ...
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bioRxiv - Genetics 2024Quote: ... 1 × 10^7 cells were processed for dead cell depletion using Dead Cell Removal Kit (Miltenyi Biotec # 130-090-101) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... then washed with 3 mL washing buffer (PBS + 1% BSA + 0.2 U/μL RNase inhibitor) and filtered through 30 μm MARC SmartStrainer (Miltenyi Biotec) twice ...
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bioRxiv - Cell Biology 2024Quote: ... Lineage depletion (B220, CD3, Gr-1, and Ter119) was performed prior to sorting using MACS magnetic microbead kits (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and astrocytes magnetically labelled with ACSA-2 microbeads (Miltenyi Biotec, 130-097-678), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Livers were collected in 2 mL autoMACS Rinsing Solution + 0.5% BSA (Miltenyi Biotec). Tubes were then rocked for 30 min at room temperature to reduce epithelial cell proportions ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 µL of Cd45-VioBlue antibody (REA737, #130-110-802, Miltenyi Biotec). After mixing well ...
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bioRxiv - Genomics 2022Quote: ... and 40 U/ml recombinant human IL-2 (Miltenyi Biotec, #130-097-745) for 2 days prior to flow cytometry analysis.
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bioRxiv - Genomics 2022Quote: ... and 40 U/ml recombinant human IL-2 (Miltenyi Biotec, #130-097-745). HEK293FT cells were a gift from the Salk viral vector core and were cultured in DMEM (high glucose ...
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bioRxiv - Immunology 2020Quote: ... anti-CD8α (Ly-2)- and anti-CD90.2-MicroBeads (Miltenyi Biotec, Bergisch-Gladbach, Germany) and then separated over an auto-MACS column using the DepleteS program.
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bioRxiv - Immunology 2020Quote: ... by adding anti-CD45RA biotin (Miltenyi Biotec, Clone REA1047, 2 uL per stain) to the antibody cocktail ...
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bioRxiv - Neuroscience 2021Quote: ... 2 µL of Cd11b-APC antibody (M1/70, #130-113-793, Miltenyi Biotec), and 2 µL of Cd45-VioBlue antibody (REA737 ...
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bioRxiv - Neuroscience 2022Quote: ... and astrocytes were isolated using the Anti-ACSA-2 MicroBead Kit (Miltenyi Biotec). RNA-sequencing was performed using TrueSeq Stranded Total RNA V2 library preparation along with next-generation sequencing on the Illumina Novaseq 6000 platform with an approximate read-depth of 90 million reads per sample ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured at 2×106 cells/ml in TexMACS medium (Miltenyi Biotec) supplemented with 10 ng/ml of IL-7 and IL-15 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 2×104 events were collected using a MACSQuant (Miltenyi Biotec) and analyzed using FlowJo v10.6.1 ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... gradient and cells were sorted using Anti-ACSA-2+ MicroBead Kit (Miltenyi Biotec). Cells were seeded onto 14 mm diameter glass coverslips in 24-well plates at a density of 20.000 cells/well for immunocytochemistry and in 6-well plates at a density of 500.000/well for qPCR ...
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bioRxiv - Genetics 2024Quote: ... for 2 days and purified with CD144 (VE-Cadherin) magnetic beads (Miltenyi Biotec) on day 6 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 IU/mL of IL-2 (Miltenyi Biotec catalog #130-097-743), seeded in 96 well flat bottom plates (Greiner Bio-One catalog #655180 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in clusters and passaged every 4-5 days using StemMACS Dissociation reagent (Miltenyi Biotec). All iPS lines were genotyped using an RFLP at the relevant important loci and the sequence was confirmed by Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... cut into 4 pieces and enzymatically digested by using the whole skin dissociation kit from Miltenyi biotec without enzyme P for 5 hours at 37°C under agitation (500 rpm) ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 50 ng/ml GM-CSF and 20 ng/ml IL-4 (Miltenyi Biotec). Untouched peripheral blood cDC1 and cDC2 (cDCs ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec). Human cells were then cytospinned with Shandon cytofunnels (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec) and isolated by positive magnetic selection according to manufacturer’s instructions (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with HBSS and spun at 300 g for 5min at 4 °C (Miltenyi Biotec). The pellet was resuspended and incubated with 80 µL of MACS buffer and 20 µL of anti-IgM microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... and stored at 4°C before being acquired using the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec) and analyzed using FlowJo 10 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed three times and incubated in PBS containing DAPI (1 μg/mL) for 15 min before samples were processed using flow cytometry (MACSQuant, Miltenyi Biotec). At least 10000 events were analyzed per sample using FlowLogic software (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
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bioRxiv - Neuroscience 2022Quote: ... The second (positive) magnetic separation was then performed using astrocyte-specific anti-GLAST (ACSA-1) antibodies conjugated to the magnetic beads (Miltenyi Biotec). Anti-O4 selection using magnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
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bioRxiv - Immunology 2020Quote: ... the pre-enriched pDC were labeled with pDC-specific CD304 (BDCA-4/Neuropilin-1) Diamond MicroBeads and isolated by positive selection over a MS MACS Column and magnetic field MACS Separator (Miltenyi Biotec). Purified pDC were then counted and tested for purity by FACS staining with mouse anti-human antibodies directed against lineage markers (VioBlue-CD3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Neuroscience 2022Quote: ... On day five ECs were dissociated with StemPro Accutase (diluted 1:6 in D-PBS) and separated via Magnetic-activated cell sorting (MACS, Miltenyi Biotec) using CD34 MicroBeads ...
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bioRxiv - Plant Biology 2022Quote: ... The blots were then incubated at room temperature with the monoclonal α-GFP antibody (1:5000) (Miltenyi Biotec, 130-091-833), α-RFP antibody (1:5000 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
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bioRxiv - Molecular Biology 2020Quote: PBMC were obtained from healthy donors and were cultured in 48-well plates at a density of 1 × 106/ml in TexMACS (Miltenyi Biotec), 3% human serum (Seralab ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM EDTA) and stained with 1 μl of an APC-conjugated CD21 specific antibody (clone: HB5; 130-101-739, Miltenyi Biotec). The cells were incubated at 4 °C in the dark for 20 min and washed with and resuspended in 200 μl FACS staining buffer ...
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bioRxiv - Immunology 2021Quote: ... sorted B cells were cultured with rh IL-21 as previously described and with Human CD40-Ligand Multimer Kit (1 µg/mL, Miltenyi Biotec), rh IL-6 (20 ...
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bioRxiv - Immunology 2021Quote: ... The right lungs as well as the right hemispheres of the brains were homogenized in 1 ml PBS using a gentleMACS Octo Dissociator (Miltenyi Biotec) and viral RNA was isolated from 140 µl cleared homogenate or BAL fluid using QIAamp Viral RNA Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... B16F10 cell lines were transfected with pRP[CRISPR]-hCas9-U6>PDPN gRNA 1 plasmid (constructed and packaged by Vector-builder; vector ID: VB160517-1061kpr) before negative selection using MACS LD columns (Miltenyi Biotec), anti-mouse podoplanin-biotin antibody (50 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were added at a dilution of 1:100 in autoMACs Rinsing Solution (Cat. No. 130-091-222, Miltenyi Biotec) containing 0.5% BSA at 4 °C for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Primary neurons where derived from neural tissue following standard operational procedure from p0-1 mice brain using the Neural Tissue Dissociation Kit-Postnatal neurons (Cat. 130-094-802, Miltenyi Biotec). After removing the meninges ...
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bioRxiv - Immunology 2020Quote: ... The numbers of cells that migrated to the lower well after 1 hr incubation were counted using a MACSQuant flow cytometer (Miltenyi Biotec). The percent migration was calculated by the numbers of neutrophils that migrated into the bottom chamber divided by the total number of neutrophils in the starting cell suspension and multiplying the results by 100 ...
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved bone marrow mononuclear cells were thawed and stained using standard procedures with the surface antibody CD34-PE (clone AC136, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor cells were transduced with a MOI of 10 for 24 h using Vectofusin-1 (10 μg/mL; #130-111-163, Miltenyi Biotec), with the following selection in Puromycin 1-2 μg/ml (#P8833 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 1 hour on ice with magnetic beads coated with antibodies against Pdgfra (Miltenyi Biotec #130-101-547). Separation of Pdgfra-positive and negative populations was performed with LS column (Miltenyi Biotec #130-042-401 ...