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Citations for Miltenyi Biotec :
651 - 700 of 822 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... hFlt3-Ligand 100ng/mL, 130-096-479; hIL-3 60ng/mL, 130-095-069; TPO 10nM, 130-094-013, all from Miltenyi Biotec) and 1% PS during 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: CD34+ cells were enriched from BM MNCs of sex-matched SF3B1-mutated MDS patients (n = 3) and healthy donors (n = 2) using CD34 Microbeads and positive selection with autoMACS Pro Separator (Miltenyi Biotec). 4000 or 7500 cells were plated as duplicates in MethoCult (H4434 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked by incubation for 15 minutes in Fc Blocking Reagent (1:100 in FACS buffer, Miltenyi Biotec, Bergisch Gladbach, Germany). Single-cell suspensions were stained with the indicated fluorescent antibodies on ice for 45 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Human myeloid CD1c+ DC were isolated from blood and kidney by positive immuno-magnetic selection using anti-CD1c/BDCA-1 microbeads according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany) or on a FACS ARIA (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 1% penicillin/streptomycin/L-glutamine)] prior to homogenisation in gentleMACS™ C-tubes using the gentleMACS™ dissociator (Miltenyi Biotec, Auburn, California, USA). Samples were incubated at 37°C with shaking to maximise digestion ...
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bioRxiv - Microbiology 2023Quote: Approximately half of the lung and the left hemisphere of each brain were weighed and homogenized in 1 mL of DMEM supplemented with 2% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells were magnetically isolated from spleens using CD8a+ negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-104-075). Purified OT-1 T-cells were resuspended in UCM ...
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bioRxiv - Developmental Biology 2023Quote: The fetal lung samples were dissociated into single-cell suspension using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec; cat # 130-110-201) and 37C Multi_tissue_dissociation B program ...
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bioRxiv - Immunology 2024Quote: The left lung was weighed and homogenized in 1 mL of RPMI supplemented with 10% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mg of microdissected vmPFC grey matter was homogenized in 2 ml of cold homogenization buffer (1M sucrose + 1% BSA dissolved in DEPC-treated water) using the benchtop gentleMACS™ Dissociator (Miltenyi Biotec, Germany) with the rotating paddle set to the Lung 02.01 program ...
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bioRxiv - Developmental Biology 2024Quote: ... in 200 μl of myeloid medium (MM) (DMEM with high glucose and L-glutamine, 10% FBS, and 1% Pen-Strep, supplemented with 50ng/ml Tpo (Miltenyi Biotec, Auburn, CA), 5ng/ml SCF ...
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bioRxiv - Cell Biology 2024Quote: Each 60 mg portion of freshly obtained skeletal muscle was suspended in 1 mL ice-cold Lysis Buffer (Mitochondria Isolation Kit, human, 130-094-532, Miltenyi Biotec, CA, USA) containing 5 μL/mL Protease Inhibitor Cocktail (P8340 ...
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bioRxiv - Molecular Biology 2021Quote: ... we stained batches of 5 million cells with 20 µL FOXA2-APC (Miltenyi Biotec #130-107-774) and 4 µL SOX17-PE (Miltenyi Biotec #130-111-032) ...
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bioRxiv - Genomics 2022Quote: ... cells were dissociated using TrypLE for 5 min and sorted using CD144-conjugated magnetic microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14, Miltenyi Biotec). After 3 hrs of specific peptide stimulation ...
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bioRxiv - Immunology 2021Quote: ... Spleens were dissociated in 5 mL PBS using a gentleMACS™ dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Following centrifugation (800xg ...
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bioRxiv - Genetics 2020Quote: ... following the manufacturer’s instruction for 30 minutes (4 °C) before being passed successively through two LS Columns (Miltenyi Biotec) with three MACs buffer washes ...
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bioRxiv - Microbiology 2020Quote: ... and the medium on the monocytes was replenished with X-vivo 15 + L-glutamine containing interleukin-4 (Miltenyi Biotec) and granulocyte-macrophage colony-stimulating factor (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... sliced into 4 sagittal sections and placed into gentleMACS C-tubes (#130-093-237, Miltenyi Biotec, San Diego, CA), and processed for generation of single-cell suspensions using the Adult Brain Dissociation Kit and GentleMACS Octo Dissociator system (#130-107-677 and #130-095-937 ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Physiology 2023Quote: ... at 4°C for 15 min under continuous agitation and passed through MS column (130-042-201, Miltenyi Biotec). The CD146 positive cells ...
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bioRxiv - Immunology 2022Quote: ... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...
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bioRxiv - Bioengineering 2021Quote: ... Lymph nodes were stained for CD19 expression using the panel described in supplemental table 1 following incubation with FcR blocking reagent (Miltenyi Biotec # 130-092-575). Cells were analyzed on a BD LSRFortessa ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were cultured for one week and then the same procedure was followed with anti-Prominin-1 MicroBeads (Miltenyi Biotec, Cat# 130-092-564) for the negative selection of radial glia ...
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bioRxiv - Neuroscience 2022Quote: ... the culture medium was replaced with a neural maintenance medium (NMM) supplemented with 1 µM StemMACS™ LDN-193189 (Miltenyi Biotec, #130-106-540) and 10 µM SB431542 (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... The enriched B cells were resuspended in 1 % BSA in PBS x1 and 2 mM EDTA and stained with anti-CD19-FITC (Miltenyi Biotec, 130-113-645), anti-igG-APC (Miltenyi Biotec ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (Miltenyi Biotec: 130-102-336) at 4 °C for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... Open-repertoire T cells (7.5e106 cells per well of a 24 well plate) were stimulated with OKT3 (50 ng mL−1, Miltenyi Biotec, Cat 130-093-387) and rhIL-2 (300 IU mL−1 ...
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bioRxiv - Neuroscience 2023Quote: ... The cerebral cortex was cleaned of meninges and the entire cell populations were isolated by enzymatic digestion and mechanical dissociation using the Multi Tissue Dissociation Kit 1 (Miltenyi Biotec, Bergisch Gladbach, GER) according to manufacturer instructions followed by a 30% density gradient centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were cut into smaller pieces and transferred into Enzyme Mix 1 (955 μl of Buffer Z, 25 μl of Enzyme P [Miltenyi Biotec, 130-094-802]) in a 1.5-ml tube ...
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bioRxiv - Immunology 2023Quote: ... cells were transduced with 2uL per well of concentrated Lenti-CRISPR-v2-GFP supernatant (transduction efficiency kept <20% to promote single copy integration) and 1uL Vectofusin-1 (1X, Miltenyi Biotec, 130-111-163) diluted in 50 uL of cord-blood optimized media ...
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bioRxiv - Plant Biology 2022Quote: ... isolated from transfected protoplasts were separated by SDS-PAGE and subjected to immunoblotting using an anti-GFP-HRP antibody (130-091-833, Miltenyi Biotec, dilution 1:5000).
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP conjugate antibody (Miltenyi Biotec 130-091-833, 1:1000), rabbit anti-golgin-45 (ab155510 ...
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bioRxiv - Bioengineering 2024Quote: ... single cell suspensions of lung cells were resuspended n 2% FBS in PBS and incubated with a 1:1000 dilution of FcR blocking reagent (Miltenyi Biotec Inc., Auburn, CA). The cells were subsequently stained for EpCAM ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HA-Bcl-2 -scFvPZ1 domain for the dual-DROP-CAR was labeled with an anti-HA antibody (1:100, Miltenyi Biotec, 130-120-791) and an anti-mouse secondary antibody (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell pellet was gently tapped to loosen it and then resuspended in 1 mL of wash buffer containing 50 μL of CD43 (Ly-48) MACS microbeads (Miltenyi Biotec, #130-049- 801). The mixture was incubated on ice for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... to label and exclude islet cells, HPx1-Dylight 488 (Novus, NBP1-18951G) to label acinar cells and CD133/1(AC141)-PE (Miltenyi Biotec 130-080-801), CD133/2(293C3)-PE (Miltenyi Biotec 130-090-853 ...