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Citations for Miltenyi Biotec :
651 - 700 of 738 citations for 1 Piperidin 4 yl azetidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Splenocytes or small intestinal IELs isolated from QFLTg or OT-1 mice were depleted of non-T cells using Pan T isolation Kit II (Miltenyi Biotec). The negatively selected T cells were then labeled with 5µM Cell Tracing Violet (CTV ...
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bioRxiv - Cell Biology 2023Quote: ... One ovary from each mouse was dissociated using a Multi Tissue Dissociation Kit 1 (cat# 130110201, Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions to create a single-cell suspension in D-PBS (cat# 14287080 ...
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bioRxiv - Bioengineering 2024Quote: Splenocytes were plated in 96-well U-bottom plates at 106 cells/well in RPMI and stimulated with 1 μg/mL of PepTivator Ovalbumin (Miltenyi Biotec). After 3 hours ...
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bioRxiv - Immunology 2022Quote: ... 0.1 mg ml−1 DNAase I in RPMI-1640 medium and enriched by depleting CD45+ cell and TER119+ cells using MACS microbeads (Miltenyi Biotec). Cells suspensions were incubated with anti-CD45 (30-F11 ...
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bioRxiv - Immunology 2023Quote: ... and 1:8 with CD8+ T cells enriched using mouse CD8 (Ly-2) Microbeads kit (Miltenyi Biotec Cat # 130-049-401) and labeled with CellTrace Violet (CTV ...
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bioRxiv - Immunology 2022Quote: ... to 1×108/ml and Ly6G+ cells were isolated according to the anti-Ly6G MicroBead Kit (#130-092-332, Miltenyi Biotec). The cells were labeled with biotinylated anti-Ly6G antibodies (anti-Ly6G-biotin ...
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bioRxiv - Molecular Biology 2023Quote: ... and rinsed once with PBS and blocked in blocking buffer (PBS with 50% FCS, FcR Blocking Reagent 1:150 (Miltenyi Biotec) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells (5×106) were infected at 5000 TCID50 / 1×106 cells at 37 °C for 2 hours on a cell-rotator (Miltenyi Biotec). After infection ...
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bioRxiv - Neuroscience 2023Quote: ... for all male cell lines (FD-1, FD-2, Ctrl) and the StemMACS mRNA reprogramming kit (Miltenyi Biotec, Bergisch Gladbach, Germany) for the female Fabry cell line (FD-3;26) ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then plated at 1-1.5×106 cells/ml with 50ng/ml human FLT3-ligand (Miltenyi Biotec; 130-096-480) and cultured for 7 days in RPMI-1640 Glutamax (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 20mg of tissue per sample was homogenized in RLT plus lysis buffer with 1% beta-mercaptoethanol using gentleMACS M-tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec). RNA extraction for both T-47D and PDX samples was performed using a Quick-RNA RNA Miniprep kit (Zymo Research) ...
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bioRxiv - Neuroscience 2023Quote: ... the suspension was counted and incubated with Anti-O4 MicroBeads at a concentration of 10 uL of beads per 1×107 cells (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Human blood samples were enriched for T cells using negative bead selection (StemCell) and stimulated in vitro in media containing a 1:100 titer of TransAct (Miltenyi Biotec), 5% FBS and 100 IU/mL of rhIL-2 ...
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bioRxiv - Molecular Biology 2023Quote: ... following which the cells were cryopreserved at a concentration of 5×106-1×107 cells/mL in CryoMACS 50 or 250 Bags (Miltenyi Biotec) for the gene edited drug product generation step.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were obtained from the lymph nodes (LNs) and spleens of OT-1 mice using a negative selection kit (130-104-075, Miltenyi Biotec). For CTL differentiation ...
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bioRxiv - Molecular Biology 2024Quote: ... iPSCs expressing the pluripotency marker TRA-1-60 were sorted and enriched using magnetic-activated cell separation (MACS) technology (Miltenyi Biotec). Cells were cultured in neural induction medium (neurobasal medium supplemented with 1x neural induction supplement (ThermoFisher) ...
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bioRxiv - Physiology 2024Quote: ... The cell strainer was washed twice and the cell suspension was centrifuged at 500g for 5 min before supernantant was removed and the pellet was frozen in 1 ml Cryobrew (cat. no.: 130-109-558, StemMACS, Miltenyi Biotec) before further analysis ...
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bioRxiv - Immunology 2024Quote: ... Tissue samples were then minced into fragments and enzymatically digested for 1 hour at 37°C into single-cell suspensions using the gentleMACS Octo dissociator and human tumor dissociation kit (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... lungs of infected mice were harvested and homogenized in 1 mL of minimal essential media with 10% bovine serum albumin using a gentleMACS C Tube (Miltenyi Biotec). Subsequently ...
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bioRxiv - Physiology 2024Quote: ... Neonatal ventricular cardiomyocytes (NRVM) were obtained by enzymatic dissociation of 1-day-old Wistar rats heart using Neonatal Heart Dissociation Kit (Miltenyi Biotec). Ventricles were cut into 1-2 mm pieces and incubated with digestive enzymes (37°C ...
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bioRxiv - Immunology 2024Quote: ... the cells were resuspended in 1 mL of MACS buffer and applied to LS columns (Miltenyi Biotec, Cat #: 130-042-401) for enriching tetramer-bound T cells ...
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bioRxiv - Immunology 2024Quote: ... The production of several major cytokines from CD4+ T cells was examined as described previously.30 Splenic CD4+ T cells (1×105 cells) isolated using MACS (Miltenyi Biotec) were stimulated with plate-bound anti-CD3 (10 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... with an end concentration of 1 µg/ml was added and the samples were measured in the MACSQant Analyzer 16 (Miltenyi Biotec). Cell Counts were analyzed with Flowlogic software.
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bioRxiv - Cancer Biology 2024Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng ml−1 mouse recombinant vascular endothelial growth factor and 20 ng ml−1 human recombinant fibroblast growth factor-2 (all from Miltenyi Biotec) as described 85 and plated on pre-coated (Collagen I (10 µg ml−1 Millipore) ...
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bioRxiv - Cancer Biology 2024Quote: Fresh spleen was collected in 3mL complete medium (CM; DMEM + 10%FBS + 1% peniciline/streptomycin) in a homogenization tube (gentleMACSTM C Tubes, Miltenyi Biotec). Spleen was dissociated using tissue dissociator gentleMACS Octo (gentleMACSTM ...
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bioRxiv - Systems Biology 2020Quote: ... approximately 1×107 cells/80 µL of Magnetic Bead Buffer were incubated with anti-PE-magnetic-activated cell sorting MicroBeads (20 µL/1×107 cells; Miltenyi Biotec, UK) for another 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed to dopaminergic patterning medium composed of CTS™ DMEM-F12 (with CTS™ N2 1:100) plus freshly added 200 ng/ml GMP Sonic Hedgehog (SHH, 130-095-727, Miltenyi Biotec), 100 ng/ml GMP FGF-8b (130-095-740 ...
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bioRxiv - Bioengineering 2022Quote: ... PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 2.4 ml of buffer RLT with 1% β-mercaptoethanol was added to the lungs from each mouse and the tissue was homogenized in an M tube (Miltenyi Biotec) using a gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinity™, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly cells were strained through a 70 μm cell strainer and then stained with CD11b (1:50 Miltenyi Biotec 130-113-800) and MEFSK4 (1:50 Miltenyi Biotec 130-120-802) ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA in PBS) and labeled with anti-mouse CD31-PE (1:50, #130-111-354, Miltenyi Biotec, Auburn, CA, USA), anti-mouse CD45-FITC (1:50 ...
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bioRxiv - Microbiology 2020Quote: Tissue samples were homogenized 2 × 55 sec in 1 ml sterile phosphate buffer saline (PBS) using a gentleMACS™ Octo Dissociator (Miltenyi Biotec). Total DNA from bacterial cells and infected tissues was purified using a GeneJet Genomic DNA Purification Kit (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... A total of 1.5 × 106 PBMCs were stimulated with 1 µg/mL of peptide pools for 6 h in TexMACS™ GMP Medium (Miltenyi Biotec), supplemented with 5% AB human serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 250 μl of each sample were incubated on ice for 1 h with 50 μl of α-GFP µMACS beads (Miltenyi Biotec). Immunoprecipitates were isolated using the μMACS GFP isolation kit ...
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bioRxiv - Microbiology 2022Quote: ... Tonsil and lung tissue samples were dissected to approximately 1 g pieces and placed into gentleMACS C Tubes (Miltenyi Biotec, Auburn, CA). The weight of each sample was recorded and PBS was added to a 10% weight/volume ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Cancer Biology 2023Quote: ... The anti-IgA-stained bacteria were then incubated in 1 mL of staining buffer containing 50 μL of anti-PE magnetic activated cell sorting (MACS®) beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of each sample was transferred to an Eppendorf tube and assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). GFP-labeled B ...
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bioRxiv - Cell Biology 2024Quote: ... an equivalent of 1,000 human CD34+ cells from mBM and hOss were plated in 1 mL of methylcellulose-based medium (130-125-042, StemMACS™ HSC-CFU Assay Kit; Miltenyi Biotec) that contained human SCF ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked by incubation for 15 minutes in Fc Blocking Reagent (1:100 in FACS buffer, Miltenyi Biotec, Bergisch Gladbach, Germany). Single-cell suspensions were stained with the indicated fluorescent antibodies on ice for 45 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Human myeloid CD1c+ DC were isolated from blood and kidney by positive immuno-magnetic selection using anti-CD1c/BDCA-1 microbeads according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany) or on a FACS ARIA (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 1% penicillin/streptomycin/L-glutamine)] prior to homogenisation in gentleMACS™ C-tubes using the gentleMACS™ dissociator (Miltenyi Biotec, Auburn, California, USA). Samples were incubated at 37°C with shaking to maximise digestion ...
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bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...