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Citations for Miltenyi Biotec :
601 - 650 of 986 citations for trans 1 2 2 Benzofuranyl 4 ethyl 1 3 dioxolan 2 yl methyl 1H 1 2 4 triazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and anti-ROR1-APC (1:25, #130-119-860, Miltenyi Biotec, Bergish Gladbach, Germany) antibodies on ice for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 1 x 106 cells in 0.04% BSA/PBS (Miltenyi Biotec 130-091-376) were used for single nuclei isolation ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 1 x 106 cells in 0.04% BSA/PBS (Miltenyi Biotec 130-091-376) were used for single nuclei isolation ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were resuspended in 1 ml 0.5% BSA (#130-091-376, Miltenyi Biotec) in D-PBS and filtered through a 35-μm filter (#352235 ...
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bioRxiv - Cell Biology 2024Quote: ... and mouse anti-GFP HRP-conjugated at 1:5,000 (130-091-833, Miltenyi Biotec). Chemiluminescence detection was conducted using SuperSignal West Femto Maximum Sensitivity Substrate (34096 ...
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bioRxiv - Genomics 2023Quote: ... the cells were resuspended in 1 ml 0.5% BSA (#130-091-376, Miltenyi Biotec) in D-PBS and filtered through a 35-μm filter (#352235 ...
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bioRxiv - Microbiology 2023Quote: ... Recovered cells were incubated with Fc block (Miltenyi Biotec, Bergusch Gladbach, Germany, 1:100), vortexed ...
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Stability and heterogeneity in the anti-microbiota reactivity of human milk-derived Immunoglobulin AbioRxiv - Immunology 2023Quote: ... APC Anti-Human IgA [Anti-Human IgA APC (Miltenyi Biotec clone REA1014, (1:50)] ...
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bioRxiv - Plant Biology 2024Quote: ... and then with antibody (1:5000 anti-His-HRP (130-092-785, Miltenyi Biotec) or anti-FLAG-HRP (Ab49763 ...
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bioRxiv - Immunology 2024Quote: ... and cultured at a density of 1×106 per mL in TexMACS (Miltenyi Biotec) media with 3% heat-inactivated human serum (Seralab ...
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bioRxiv - Genetics 2024Quote: ... samples were incubated with 3µg anti-GLAST (ACSA-1) biotinylated antibody (Miltenyi Biotec, USA) and BSA (3% ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated anti-GFP (1:5,000; Miltenyi Biotec) antibodies for one hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... BM cells were also stained with human CD3 (1/50 CD3-APC; Miltenyi Biotec), human CD14 (1/50 CD14- PECy7 ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: ACSA2 APC (1:200, Miltenyi Biotec, 130-116-245) and O4 PE (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stored at 4°C in dark until the next morning for data acquisition using a MacQuant10 (Miltenyi Biotec, USA). Data was analyzed using FlowJo 10 (BD Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2020Quote: ... DC differentiation was carried out over 6 days with 250 U/ml IL-4 and 50ng/ml GM-CSF (Miltenyi Biotec).
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bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... The adherent monocytes were then washed twice with PBS and resuspended in RPMI supplemented with the cytokines GM-CSF (1000 U/mL) and IL-4 (500 U/mL) (Miltenyi Biotec). Differentiation to immature MDDCs was achieved by incubation at 37 °C with 5% CO2 for 7 days and subsequent activation with cytokines addition every other day ...
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bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
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bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting cell suspension was spun down for 5 min at 300 × g and 4°C and processed for MACS using the CD11b/c microbeads according to the manufacturers protocol (Miltenyi Biotec). Subsequently ...
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with the following antibodies for 30 minutes at 4°C: CD3 VioGreen (clone BW264/56, Miltenyi Biotec), CD14 V500 (clone M5E2 ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured with 100 ng/mL (500 UI/mL) GM-CSF plus 50 ng/mL (250 UI/mL) IL-4 (Miltenyi Biotec) at 106cells/mL in complete RPMI medium supplemented with 10% FCS (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were collected and centrifuged at 500 x g for 5 min at 4°C and CD8 T cells were isolated by magnetic sorting according to the manufacturer’s protocol (Miltenyi Biotec, Germany). To select transduced CD8 T cells ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was carried out for 30 min in the dark at room temperature with anti-4-1BB-PE (Miltenyi Biotec), anti-CD40L- PEVio770 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... CD8 T cells from autologous blood and tonsils from 4 HLA-A*02:01 donors were enriched using negative selection magnetic beads (CD4, CD19 depletion, Miltenyi Biotec). Roughly 1.5 e6 enriched CD8s were stained with Fc block for 10 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... 4 days later peritoneal washes were collected and F4/80+ macrophages were sorted with magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) with the typical purity yield >95%.
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated using Ficoll-Hypaque density centrifugation and pDCs were positively selected from PBMCs using BDCA-4-magnetic beads (MACS Miltenyi Biotec), as previously described (Decembre et al. ...
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bioRxiv - Microbiology 2024Quote: ... Harvested cells were centrifuged at 300×g for 5 min at 4°C and resuspended in red blood cell lysis buffer (#130-094-183, Miltenyi Biotec) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... 2 to 8 tubes (4 to 16 slices) of frozen tissue were dissociated using GentleMACS nuclei isolation protocol (nuclei isolation buffer [Miltenyi Biotec, cat# 130-128-024] ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated the samples for a total of 1h at RT with intermittent pulses of mechanical tissue disruption using the gentleMACSTM tissue homogenizer (Miltenyi Biotec). The resulting homogenate was washed twice using 10 ml of ice-cold 1% BSA/PBS and pelleted for 5min at 800g (4°C) ...
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bioRxiv - Immunology 2020Quote: ... anti-CD66abce-PE-Vio770 (clone TET2; 1 μL; cat. # 130-119-849) from Miltenyi Biotec; and anti-CD27-PE-Cy5 (clone 1A4CD27 ...
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bioRxiv - Neuroscience 2020Quote: ... plus 20μl anti-GLAST (ACSA-1) MicroBeads (Miltenyi Biotec, Cat# 130-095-825, Auburn, CA) for up to 107 cells ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-human CD38 (IB6, PE, Miltenyi Biotec, Cat No 130-113-989, 1:50) antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Penstrep) or adipogenic (Adipogenic differentiation media; ADM, Cat. no. 130-091-677, Miltenyi Biotec) linage ...
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bioRxiv - Neuroscience 2022Quote: ... Live surface marker staining with Anti-TRA-1-60 488 Live cell stain (Miltenyi Biotec) was performed at day 21 post-lipofection to identify undifferentiated iPSC colonies in the culture ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... Cells were incubated with FcR Blocking Reagent (Miltenyi Biotec; 1 μL in 50 μL MACS) for 20 minutes and then with a blood antibody cocktail for 30 minutes in the dark at 4 degrees ...
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bioRxiv - Immunology 2022Quote: ... an isotype control antibody was used (1:50 dilution, REA control antibody, Miltenyi Biotec, Germany). Samples were analysed using a flow cytometer (Becton Dickinson FACSCalibur) ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4, Miltenyi Biotec, Germany). All flow cytometry analyses were assessed using the following antibody clones ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with a 1:20 dilution of Streptavidin MicroBeads (Miltenyi Biotec), and incubated with shaking for 30 minutes at 4 ⁰C ...
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bioRxiv - Microbiology 2021Quote: ... a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812, MACS Miltenyi Biotec), 0.14 µg/mL PE-conjugated mouse anti-HLA-DR (clone LN3 ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently incubated with anti-CD117/c-kit-APC (1:50; Miltenyi Biotec; RRID: AB_615056). Cells were fixed in 1% formalin and analyzed on a BD FACS Canto II running the FACS Diva Software (BD Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: ... The following antibodies were applied: anti-CD3-FITC (1:25, Miltenyi Biotec, #130-098-162), anti-CD14-FITC (1:25 ...