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Citations for Miltenyi Biotec :
601 - 650 of 1015 citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14, Miltenyi Biotec). After 3 to 4 hrs of specific peptide stimulation ...
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bioRxiv - Immunology 2020Quote: ... A 1:1 mixture of CD3-depleted (Miltenyi Biotec) Tollip-/- (CD45.2+ ...
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bioRxiv - Cell Biology 2023Quote: ... insulin-like growth factor 1 (IGF-1, Miltenyi Biotec), FGF-2 and LDN193189 ...
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bioRxiv - Immunology 2021Quote: ... CD3 (17A2; 1:400 or REA641; Miltenyi Biotec; 1:150); CD122 (TM-β1 ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... and on day 5 after start of gametocyte induction magnetic enrichment was performed with MACS columns (Miltenyi Biotec). Gametocytes were sorted on a BD FACSAria III on day 8 ...
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bioRxiv - Neuroscience 2023Quote: ... sample and collection tubes were placed in a cold MACS Chill 5 Rack (#130-092-951, Miltenyi Biotec) with both positive and negative fractions being collected ...
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bioRxiv - Neuroscience 2023Quote: ... cells were cultured in medium supplemented with 5 μM of the ROCK inhibitor StemMACS™ Y27632 (Miltenyi Biotec). The differentiation of hiPSCs into midbrain neurons was performed as previously described (35) ...
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bioRxiv - Immunology 2021Quote: ... CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec #130-104-075) and then mixed with gp33-pulsed B cells at a ratio of 1:1 and incubated for indicated times at 37°C.
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bioRxiv - Immunology 2019Quote: ... For genomic and transcriptomic analyses of activated LCs fresh migrated LCs from 3 donors were stimulated with TNF-α (25 ng/ml, Miltenyi Biotec, UK) for 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein complexes were then eluted into 1.5 mL Eppendorf tubes using 3 x 35 µL of pre-heated 95°C elution buffer (Miltenyi Biotec #130-042-701). The samples were heated to 95°C for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... in 5 mL of IMDM+Glutamax medium for 30 min at 37°C in gentleMACS C tubes (Miltenyi Biotec). During and after incubation ...
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bioRxiv - Immunology 2022Quote: ... Cells were then pelleted and resuspended in 5 mL PBSF and sorted using a MidiMACS Separator magnet (Miltenyi Biotec) in combination with an LS column (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... 5 mL of FACS buffer was added and the samples were passed over a magnetized LS column (Miltenyi Biotec). The tube and column were washed once with 5 mL FACS buffer and then removed from the magnetic field ...
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bioRxiv - Immunology 2022Quote: ... 5 mL of FACS buffer was added and the samples were passed over a magnetized LS column (Miltenyi Biotec). The tube and column were washed once with 5 mL FACS buffer and then removed from the magnetic field ...
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bioRxiv - Cell Biology 2024Quote: ... NM + 1 μM RA + 1 μΜ Purmorphamine (Miltenyi Biotec 130-104-465), was added and changed every other day until D17 ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 × 106 cells/ml) and purified by magnetic cell sorting using anti-O4 MicroBeads (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Late-stage Dd2 parasites in RBCs were magnetically purified from 5% sorbitol-synchronized cultures using MACS LD columns (Miltenyi Biotec) and seeded at 0.8-million RBCs/well in a Seahorse miniplate which was precoated with Cell-Tak cell and tissue adhesive (Corning) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed with magnetic cell-sorting (MACS) buffer (PBS supplemented with 0.5% BSA and 5 mM EDTA) and incubated with CD117 (KIT) MicroBeads (Miltenyi Biotec) on ice for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... lungs and spleens were collected in 5 ml MACS Tissue storage solution (Cat: 130-100-008; Miltenyi Biotec, Gaithersburg, MD) followed by storage at 4 °C until processing ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice using PBS containing 0.05% BSA at 1,700 RPM for 5 minutes and processed in a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec, CA).
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were isolated and homogenized in STKM buffer (250 mM Sucrose, 50 mM Tris-HCl, 25 mM KCl, 5 mM MgCl2) using the GentleMACS (Miltenyi Biotec) program Protein_01 ...
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bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed in PBS and cultured for 5 days in serum-free DMEM containing 50 ng/ml BDNF (Miltenyi Biotec Cat# 130-096-285 ...
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bioRxiv - Immunology 2021Quote: ... and transplanted with syngeneic BALB/c bone marrow cells (5 × 106) after T-cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). For retransfer experiments ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.
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bioRxiv - Immunology 2022Quote: ... washed with PBS (Phosphate Buffered Saline) containing 5% FBS (Fetal Bovine Serum) then labeled with anti-PE microbeads (Miltenyi Biotec Inc.) for 15 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... After another incubation of 30 minutes at 37°C with 5% CO2 the samples are analysed by flow cytometry (MACS Quant, Miltenyi Biotec) and FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Immunology 2022Quote: All cell culture was performed in a humidified incubator with 5% CO2 at 37°C using cRPMI (see above for ingredients) supplemented with 150ng/mL αCD40 (clone FGK-45; Miltenyi Biotec) and 25ng/mL recombinant murine IL-4 (Peprotech ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... perfused murine lungs were placed in a gentle MACS C tube and mechanically homogenized in 5 ml of PBS using a gentle MACS Octo Dissociator (Miltenyi Biotec). Lung cell suspensions were lysed of RBCs ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were stained with 5 μg/ml of propidium iodide in PBS and analyzed using a MACSquant analyzer (Miltenyi Biotec). Results were analyzed using Flowjo (https://www.flowjo.com).
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bioRxiv - Molecular Biology 2024Quote: ... and both the sample and collection tubes were placed in a cold MACS Chill 5 Rack (130-092-951, Miltenyi Biotec). Both positive and negative fractions were collected using the separator ...
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bioRxiv - Cancer Biology 2023Quote: ... APC antihuman CD271 (Clone ME20.4-1.H4, dilution 1/10, Miltenyi Biotec, Paris, France) and Brilliant Violet 510™ anti-human CD45 (clone HI30 ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions of mouse lungs were generated by putting lungs in gentle MACS™ C tubes and mechanically homogenizing in 5 ml PBS using a gentle MACS™ Octo Dissociator (Miltenyi Biotec) in the absence of enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... TRA-1-60+ iPSCs were isolated from cultures using anti-TRA-1–60 MicroBeads (Miltenyi Biotec) and LS columns (Milteny Biotec) ...
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bioRxiv - Genomics 2020Quote: ... 1:100 B27 supplement (Miltenyi Biotec) and 1:40,000 Insulin (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... GLAST (clone ACSA-1, Miltenyi Biotec). Cells were incubated with antibodies for 15 minutes ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were isolated from at least 5×106 PBMCs on the day of thaw by negative selection using the CD4+ T cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Oligodendrocyte precursor cells (OPCs) were isolated from 5–7-day old YACQ84 transgenic mice using magnetic cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany). Whole brains were dissected and homogenized using the Neural Tissue Dissociation Kit P (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Genetics 2022Quote: ... in a cell culture incubator (37 °C; 5% CO2) followed by dissociation in gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in a total of 8 ml DMEM media with 10% FBS using gentleMACS Dissociator Program A twice and seeded onto 15 cm tissue culture dishes and grown in DMEM with 10% FBS at 37 °C with 5% CO2.
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-PE (Miltenyi Biotec, 1:500 dilution), rabbit anti-TCAB1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-Glast (Miltenyi Biotec, 1:100), rat anti-CD133 (eBioscience ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% BSA (Miltenyi Biotec, 130-091-376), 1 mM DTT (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... APC-CD95-Fas (Miltenyi Biotec, 1:100). DAPI was used to stain the dead cells (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... APC-CD133 (13A4, Miltenyi Biotec, 1/75), APC-Cy7-CD45 (BD ...