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Citations for Miltenyi Biotec :
551 - 600 of 1884 citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and seeded at a density of 19,000 cells/cm2 on Matrigel in mTeSR1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec). Upon reaching 95% confluence ...
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bioRxiv - Genomics 2020Quote: ... 3×108 cells were resuspended in 3 ml of FACS buffer (PBS with 10% FBS) and stained with 0.9 µg anti-ter119-PE (130-102-338; Miltenyi Biotec). Stained cells were conjugated to anti-PE microbeads (130-048-801 ...
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bioRxiv - Immunology 2019Quote: ... before culturing in a 1:10 ratio with 5×105 BALB/c splenic APCs (prepared via Thy1.2+ cell depletion by MACS, Miltenyi Biotec) in a 96-well round-bottom plate in complete RPMI medium for 5 days ...
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bioRxiv - Neuroscience 2021Quote: Cells were pelleted at 300 x g for 10 minutes at 4ºC and resuspended in 90 µL of 0.5% BSA in D-PBS with 10 µL of CD11b (Microglia) MicroBeads (#130-093-636, Miltenyi Biotec) per 107 total cells ...
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bioRxiv - Developmental Biology 2021Quote: ... The stroma was negatively selected against epithelium using a EPCAM antibody coated microbeads (1:10; Miltenyi Biotec #130-061-101) and a Miltenyi MS column.
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bioRxiv - Immunology 2021Quote: ... 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells (Miltenyi Biotec, Cat#130-104-454) from Bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis at indicated time points ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in sorting buffer and incubated with ACSA-2 MicroBeads (1:10, Miltenyi Biotec, 130-097-678) for 15 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Image sections with a step size of 10 μm were generated by Imspector Pro software (v7.1.15, Miltenyi Biotec Gmbh, Germany) and stitched together using the implemented TeraStitcher script (v.9) ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed in a 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Genomics 2023Quote: Singlet rate was estimated on dissociated tissues using flow cytometry on a MACSQuant Analyzer 10 (Miltenyi Biotec, 130-096-343). Flow cytometry data was analyzed using Flowlogic v8 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: Hippocampal cells from C57BL6/N mice were pelleted at 300 x g for 10 minutes at 4°C then resuspended in 90 µL of 0.5% BSA in D-PBS and 10 µL of CD11b (Microglia) MicroBeads (#130- 093-634, Miltenyi Biotec) followed by thorough mixing ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampal single cell suspensions were pelleted at 300 × g for 10□min at 4°C and resuspended in 90□μl of 0.5% BSA in D-PBS with 10□μl of CD11b (Microglia) MicroBeads (#130-093-636, Miltenyi Biotec) per 107 total cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice using PBS containing 0.05% BSA at 1,700 RPM for 5 minutes and processed in a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec, CA).
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bioRxiv - Immunology 2023Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Male pups were used for the experiments and injected intra-peritoneally (i.p.) twice a day from P1 to P5 with 10 μg/kg IL-1β (Miltenyi Biotec) diluted in 5 μL 1X PBS or with 5 μL 1X PBS alone [29 ...
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bioRxiv - Genomics 2024Quote: The obtained homogenate was filtered in 3-5 ml of Wash Buffer (1x DPBS, containing 10% FBS, 20mM HEPES, and 6mM glucose) through a prewetted 70μm cell culture filter (Miltenyi Biotec) and centrifuged for 5 minutes at 300xg (4 °C).
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bioRxiv - Cell Biology 2020Quote: ... and stimulated for 18 h with IL-2 (20 ng/ml; National Cancer Institute Preclinical Repository) and/or IL-12 (10 ng/ml; Miltenyi Biotec) cytokines ...
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bioRxiv - Cancer Biology 2022Quote: ... each blood sample was diluted in a 1 to 1 ratio with the HBSS buffer solution and incubated with 10% EpCAM MicroBeads (Miltenyi Biotec) with shaking at room temperature for an hour ...
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bioRxiv - Immunology 2021Quote: ... T cell activation by peptide was assessed according to the expression of eGFP regulated by the NFAT promoter and analysed on a MACSQuant Analyzer 10 (Miltenyi Biotec). The acquired data was processed by FlowJo (version 10.6.2 ...
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bioRxiv - Immunology 2019Quote: ... (at 1:20-1:100 dilutions depending on the antibody) in the presence of human Fc receptor blocking reagent (1:10) (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... All sample volumes were adjusted to 100 µl with 1x PBS + 10 µM ZnCl2 and measured via flow cytometry (MACSquant VYB, Miltenyi Biotec). The flow cytometer was calibrated using MacsQuant calibration beads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... were obtained by 7 days stimulation with 20 ng ml−1 macrophage colony-stimulating factor (M-CSF) and 10 ng ml−1 granulocyte-macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec).
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bioRxiv - Microbiology 2022Quote: ... BSA in PBS + 0.2 mM EDTA (FACS buffer) and subsequently resuspended in 100 μl FACS buffer containing 10 % (v/v) FcR blocking reagent (Miltenyi Biotec) and 1 % (v/v ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... the corresponding mAbs were added for 30 minute and cells were washed with BD Perm/Wash Buffer 1X before acquisition in the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). The percentage of positive cells for CD107a ...
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bioRxiv - Immunology 2020Quote: ... Sorted cell subsets were co-cultured at a ratio 1:1 in 200µL RPMI/10% FBS and stimulated with CytoStim™ (Miltenyi Biotec). After 7 days’ culture ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were then washed twice prior to resuspension in FACS buffer and acquisition on the MACSQuant Analyzer 10 (Miltenyi Biotec). Data were analyzed with FlowJo software (v10 ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was washed with DMEM/10% FBS followed by CD11b positive selection (Miltenyi Biotec, Cat. No. 130–093-636) using mini-MACS (Miltenyi Biotec ...
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bioRxiv - Genomics 2020Quote: ... The cell suspension is pipetted through Falcon tubes with cell-strainer caps and then analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec) for 10,000 events.
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 15 min incubation with 10 µL microbeads mixture containing antibodies directed against CD140 (130-101-502, Miltenyi Biotec), NG2 (130-097-170 ...
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bioRxiv - Neuroscience 2023Quote: ... The pelleted MVs were incubated with 10 μl of Biotin-conjugated anti-CD105 (Miltenyi Biotec cat# 130-094-916, RRID: AB_10828348) followed by adding 10 μl of anti-Biotin microbeads (MiltenyiBiotec cat# 130-094-256 ...
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bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
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bioRxiv - Immunology 2022Quote: ... in 96-well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). CD34+ HSPCs isolated from the BM of xenografted mice sacrificed between weeks 1-5 after grafting were directly seeded by FACS in the 96-well plates and cultured for 7 or 14 days before quantification of lineage outputs ...
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bioRxiv - Immunology 2022Quote: ... in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). UCB or BM CD34+ HSPC subsets were directly seeded by FACS in the corresponding plates and cultured for 10 (bulk cultures ...
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bioRxiv - Neuroscience 2023Quote: Analyses of the cells labeled in the previous section were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). Post-sort purity was assessed by the percent of CD11b+CD45+ singlets using MACSQuantify v2.13.0 software ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed twice and resuspended in 1mL of sorting buffer (up to 10⁸ cells) prior to magnetic separation on LS columns and the MACS Separator (Miltenyi Biotec). Columns were rinsed with 0.5mL of MACS buffer prior to loading the cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then centrifuged for 10 min at 3000 x g in the presence of debris removal solution (Miltenyi Biotec) and 10 min at 1000 x g in cooled PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... the suspension was counted and incubated with Anti-O4 MicroBeads at a concentration of 10 uL of beads per 1×107 cells (Miltenyi Biotec). After incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... 5 % CO2 after which migrated cells in the bottom chamber were collected and acquired on a MACSQuant Analyzer 10 Flow Cytometer using the MACSQuantify software (Miltenyi Biotec). The percentage of specific migration was calculated by dividing the number of cells migrated to the lower well by the total cell input (50 µl cell suspension directly measured on the MACSQuant Flow Cytometer).
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and labeled with 10 μL streptavidin MACS microbeads (Miltenyi Biotec, 130-048-102) (sufficient for 1e7 cells) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with Live/Dead dye and analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, San Diego, CA).
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bioRxiv - Cancer Biology 2021Quote: ... The dissociation was performed utilizing the MACS Tumor Tissue Dissociation Kit (Miltenyi Biotec) for the tumor core samples or the Lung Dissociation Kit (Miltenyi ...
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bioRxiv - Cell Biology 2020Quote: ... followed by positive selection with CD31 MicroBead Kit (130-091-935, Miltenyi Biotec) using a magnetic cell separation device (QuadroMACS Separator;130-090-976 ...