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Citations for Miltenyi Biotec :
551 - 600 of 2984 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Neuroscience 2023Quote: ... Monocytes were then isolated using 250 μL of MicroBeads-conjugated human CD14 antibodies (StraightFrom LRSC CD14 MicroBead Kit; Miltenyi Biotec) added to 40 mL of cell suspension ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in MACS buffer (PBS, 0.5% BSA, 2 mM EDTA) for CD34+ magnetic selection using CD34 human MicroBeads (Miltenyi Biotec; cat ...
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bioRxiv - Biochemistry 2023Quote: Human primary monocytes were isolated from healthy donors using the autoMACS Pan Monocyte Isolation Kit (Miltenyi Biotec, 130-096-537). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... All samples were blocked for non-specific binding with FcR Blocking Reagent for human (Miltenyi Biotec, cat.: 130-059-901) and mouse (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... B cells and T cells (Miltenyi Biotec) were used ...
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bioRxiv - Cell Biology 2019Quote: ... for no longer than 1 h before their enzymatical and mechanical dissociation with the Whole Skin Dissociation kit for human material (Miltenyi Biotec) and the Gentle MACS dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: Mouse and human brain samples were collected and mechanically dissociated using gentleMACS dissociation following the manufacturer’s instructions (Miltenyi Biotec, Paris, France). Freshly isolated tumor tissues were suspended in 5ml DMEM immediately after being surgically removed and placed in a petri dish ...
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bioRxiv - Cell Biology 2019Quote: ... the cells of one donor were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... (at 1:20-1:100 dilutions depending on the antibody) in the presence of human Fc receptor blocking reagent (1:10) (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed with PBS 1X and stained for 30 minutes using CD38 PE (IB60) Mouse Anti-Human (Cat.#130-092-260, Miltenyi Biotec)/CD38 FITC Multiepitope (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Immunology 2022Quote: The tissue pieces were rapidly transferred into the gentleMACS C Tube containing Human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec, Auburn, CA) and used immediately for further differentiation into macrophages [25] ...
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bioRxiv - Biochemistry 2019Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL) followed by CD14+ MACS magnetic bead separation (Miltenyi Biotec). The isolated monocytes were differentiated for 7 days in IMDM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: Human iPSC/ESCs (Table S1) were maintained on dishes coated with Matrigel in mTeSR1 or StemMACS iPS-Brew XF (Miltenyi Biotec) media with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... sorted B cells were cultured with rh IL-21 as previously described and with Human CD40-Ligand Multimer Kit (1 µg/mL, Miltenyi Biotec), rh IL-6 (20 ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
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bioRxiv - Cell Biology 2020Quote: ... which were then dissociated with a tumor dissociation kit (Human) using a mechanical–chemical–mechanical cycle in a gentleMACS Dissociator (Miltenyi Biotec), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: Immunoaffinity isolation of uEVs was performed using a commercially available kit (Exosome Isolation Kit Pan, human, Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: Dissociation of fresh Fallopian tube epithelium was done using the Milltenyi gentleMACS Octo Dissociator with heaters and the human Tumor Dissociation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of CD81- (for all samples) or CD63- (for MS and HC samples) isolation beads (Exosome Isolation Kit, human Miltenyi Biotec) were added to 2 ml of diluted sample ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C for 30 mins were added to fresh human neutrophils extracted from blood from healthy volunteers using MACSxpress kit (Miltenyi Biotec) at an MOI of 1:100 (72 ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were extracted in a laminar flow hood under sterile conditions and were immediately digested using the human tumor dissociation kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: The omental tumors harvested from C57Bl/6 mice were minced and dissociated using the Human Tumor Dissociation Kit from Miltenyi Biotec, following the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... and the cells were resuspended in 200 μl of flow cytometry buffer and incubated with 15 μl of human FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... human (130-050-501) combinations based on the Mini & MidiMACS™ Starting Kit (130-091-632) (all from Miltenyi Biotec, Germany) in accordance with the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...
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bioRxiv - Immunology 2023Quote: ... New granulomas determined by PET-CT and uninvolved lung lobes (no granuloma present in the lobe) were enzymatically homogenized using a human tumor dissociation kit (Miltenyi Biotec) and a gentleMACS Dissociator (MiltenyiBiotec ...
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bioRxiv - Cancer Biology 2024Quote: ... and cryopreserved in FBS supplemented with 10% dimethyl sulfoxide (DMSO) at -80 °C (samples for genotyping and mass cytometry) or processed directly using Tumor Dissociation Kit (human, Miltenyi Biotec) as described below (fresh sample for mass cytometry) ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were filtered with a 70 μm cell strainer and dead cell removal was performed on single cell suspensions (Miltenyi Biotec) and live tumor cells were frozen in 90% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Immunology 2023Quote: B-cells were isolated from lymph nodes of 5c.c7 αβ TCR-transgenic mice and enriched using a murine B-cell isolation kit in order to deplete activated B-cells and non-B-cells (B-cell Isolation Kit mouse, Miltenyi Biotec). B-cells were cultured at 37 °C and 5% CO2 in 1640 RPMI (Life technologies ...
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bioRxiv - Immunology 2021Quote: ... CD14+ cells were isolated by magnetic activated cell sorting (CD14+ Cell Isolation Kit; Miltenyi Biotec). 2×10⁵ monocytes were then differentiated into M1-type macrophages cells by incubation with 1000 U/ml GM-CSF (granulocyte macrophage-colony stimulating factor ...
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bioRxiv - Microbiology 2021Quote: ... Detection occurred by using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinity™, # 130-101-343, Miltenyi Biotec), SARS-CoV-2 N (1:1000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumors were cut in small pieces of approximately 2 mm3 then incubated with Human tumor dissociation kit (Miltenyi Biotec, #130-095-929) and placed at 37°C in agitation (500 rpm ...
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bioRxiv - Cell Biology 2022Quote: Mouse scars and human stimulated skin and scar samples were digested in Miltenyi Whole Skin dissociation Kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) for 2.5h according to the manufacturer’s protocol and processed on a GentleMACS OctoDissociator (Miltenyi) ...
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bioRxiv - Cancer Biology 2022Quote: ... Red cell lysis and dead cell removal (Miltenyi Biotec) was carried out prior to generation of single cell droplets with 10X Genomics Chromium Controller ...
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bioRxiv - Genomics 2020Quote: ... dead cells were removed from the cell suspensions using the Dead Cell Removal Kit (Miltenyi Biotec), and cells were counted again to determine final viable cell numbers.
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bioRxiv - Genomics 2019Quote: ... dead cells were removed from the cell suspensions using the Dead Cell Removal Kit (Miltenyi Biotec) and cells were counted again using an automatic cell counter.
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bioRxiv - Cancer Biology 2024Quote: ... the cell suspension was enriched for viable cells using the dead cell removal kit (Miltenyi Biotec) following the manufacturers protocol ...
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bioRxiv - Genetics 2020Quote: ... NK cell populations using cell-type specific kits (Miltenyi Biotec). For NK cells ...
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bioRxiv - Immunology 2019Quote: Peripheral blood neutrophils from human blood were purified using the Miltenyi Biotech MACSxpress Neutrophil Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec, #130-104-434) and residual red blood cells were lysed using MACSxpress Erythrocyte Depletion Kit (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2021Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL, Melbourne, Australia) followed by CD14+ MACS separation (Miltenyi Biotec, Sydney, Australia). The isolated monocytes were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... PBMC were thawed and monocytes and peripheral blood lymphocytes (PBL) were separated using human anti-CD14 Ab-coated microbeads to positively select monocytes (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...