Labshake search
Citations for Miltenyi Biotec :
551 - 600 of 872 citations for Mono 2 Ethyl 5 Carboxypentyl Phthalate Unlab. Dehp Metabolite V ;100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: ... before culturing in a 1:10 ratio with 5×105 BALB/c splenic APCs (prepared via Thy1.2+ cell depletion by MACS, Miltenyi Biotec) in a 96-well round-bottom plate in complete RPMI medium for 5 days ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Late-stage Dd2 parasites in RBCs were magnetically purified from 5% sorbitol-synchronized cultures using MACS LD columns (Miltenyi Biotec) and seeded at 0.8-million RBCs/well in a Seahorse miniplate which was precoated with Cell-Tak cell and tissue adhesive (Corning) ...
-
bioRxiv - Developmental Biology 2021Quote: ... cells were washed with magnetic cell-sorting (MACS) buffer (PBS supplemented with 0.5% BSA and 5 mM EDTA) and incubated with CD117 (KIT) MicroBeads (Miltenyi Biotec) on ice for 20 min ...
-
bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed twice using PBS containing 0.05% BSA at 1,700 RPM for 5 minutes and processed in a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec, CA).
-
bioRxiv - Cell Biology 2019Quote: ... Supernatant was incubated with 50 mL magnetic µMACS Anti-DYKDDDDK MicroBeads (Miltenyi Biotec) for 30 minutes on ice ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 1 ng/mL granulocyte macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec). Primary cell cultures were maintained in the presence of mouse embryonic fibroblasts (STO ...
-
bioRxiv - Microbiology 2020Quote: ... and 50 ng/ml rh interleukin (IL)-4 (130-093-921, Miltenyi Biotec)] and cultured at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... B cells were stimulated with 1 μg/ml CpG oligodeoxyribonucleotide 2006 (Miltenyi Biotec), 2.4 μg/ml F(ab’)2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L ...
-
bioRxiv - Microbiology 2021Quote: ... TNF-α (final conc. was 20 ng/mL for 30 min; Miltenyi Biotec), hydrogen peroxide (H2O2 ...
-
bioRxiv - Neuroscience 2022Quote: ... MACS NeuroBrew-21 with retinoic (10 mL, MACS Miltenyi Biotec, 130- 093-566), PD0332991 (100 µL ...
-
bioRxiv - Neuroscience 2022Quote: ... and 50ng/mL transforming growth factor β1 (TGFβ1; Miltenyi Biotec # 130-108-969) (MBM + 3GFs) ...
-
bioRxiv - Systems Biology 2023Quote: ... cells were eluted with 4 mL Whole Blood Column Elution Buffer (Miltenyi Biotec) and centrifuged at 500 g for 10 minutes at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 50 IU/ml premium-grade human IL2 (Miltenyi Biotec, 130-097-746) at 1x106 cells per mL and cm2 ...
-
bioRxiv - Cell Biology 2024Quote: ... and 4 ng/mL of recombinant basic fibroblast growth factor (Miltenyi Biotec, Germany). Cells were cultured for 2 passages at a seeding density of 5×10 cells/cm.
-
bioRxiv - Molecular Biology 2023Quote: ... was supplemented with 10 ng/ml IL-7 (#130-093-937, Miltenyi Biotec) and 3 ng/ml IL-15 (#130-093-955 ...
-
bioRxiv - Cell Biology 2023Quote: ... with extra supplementation of 40 ng/ml Stem Cell Factor (SCF; Miltenyi Biotec) and 1 ng/ml IL-3 (R&D Systems ...
-
bioRxiv - Neuroscience 2024Quote: ... with the addition of SHH-C24II (200 ng/ml, Miltenyi Biotec, 130-095) between days 7 and 10 of the differentiation protocol ...
-
bioRxiv - Immunology 2024Quote: ... supplemented with 10 ng/ml of IL-7 and IL-15 (Miltenyi Biotec) and 1% P/S (Biowest ...
-
bioRxiv - Immunology 2021Quote: ... The cell fraction was then depleted with human CD45 beads (100ul beads per 2×108 cells; Miltenyi Biotec) using autoMACS ...
-
bioRxiv - Cancer Biology 2022Quote: ... and then blocked in FACS buffer (PBS, 2% BSA) containing FcR blocking reagent at 1:50 (Miltenyi Biotec) for 15 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... 4 °C and stained with the APC-labelled ACSA-2 antibody (1:10, Miltenyi Biotec, 130-102-315) in 0.5 % BSA in PBS ...
-
bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
-
Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Cell samples were fluorescently stained with Anti-ACSA-2-PE (clone: IH3-18A3) (130-102-365, Miltenyi Biotec), examined by a BD FACSCanto II flow cytometer ...
-
bioRxiv - Physiology 2022Quote: Primary cardiac fibroblasts were isolated from 2-day-old rats using a neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Enzyme Mix 2 (15 μl of Buffer Y, 7.5 μl of Enzyme A [Miltenyi Biotec, 130-094-802]) was added for another 5-min ...
-
bioRxiv - Neuroscience 2023Quote: ... APC rat anti-mouse ACSA-2 (cat # 130-117-535, Miltenyi Biotec, clone IH3-18A3, 1:25 dilution), AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82 ...
-
bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2021Quote: ... and cocultures were separated with the optimized amount of Anti-Fibroblast MicroBeads (40 μL, for up to three 100 mm Petri Dishes, Miltenyi Biotec) according to the manufacturer’s protocol to reach minimal contamination in each compartment (Fig ...
-
bioRxiv - Immunology 2021Quote: ... the cells were fixed for 20 min at RT with 250 μL of the Inside Fix solution then washed and stained in 100 μL of the Inside Perm solution containing PE anti-TNF (clone cA2, Miltenyi Biotec) and APC anti-IL-1β (clone REA1172 ...
-
bioRxiv - Immunology 2019Quote: ... (at 1:20-1:100 dilutions depending on the antibody) in the presence of human Fc receptor blocking reagent (1:10) (Miltenyi Biotec) for 15 min at 4°C ...
-
bioRxiv - Synthetic Biology 2021Quote: ... All sample volumes were adjusted to 100 µl with 1x PBS + 10 µM ZnCl2 and measured via flow cytometry (MACSquant VYB, Miltenyi Biotec). The flow cytometer was calibrated using MacsQuant calibration beads (Miltenyi Biotec ...
-
bioRxiv - Physiology 2021Quote: ... The pellets were resuspended in 100 μl beads buffer consisting of 80 μl sorting buffer with 20 μl secondary Anti-rabbit IgG MicroBeads (Miltenyi Biotec) and incubated for 15 min in at 4 °C sorting buffer (900 μl) ...
-
bioRxiv - Cancer Biology 2022Quote: ... the supernatant was removed and the cells were resuspended with 100 µl of Inside Perm Buffer (Inside Stain Kit, Miltenyi Biotec). Eight µl of anti-GFAP and 2.5 µl of anti-Iba1 were added to the cell suspensions and mixed by gently pipetting ...
-
bioRxiv - Cell Biology 2022Quote: ... Biopsies were manually homogenized using sharp dissection between digest steps and by blunt homogenization through a 100 μm cell strainer (Miltenyi Biotec). Centrifugation steps were performed at 300 x g for 5 min at 4°C.
-
bioRxiv - Cell Biology 2022Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...
-
bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were purified using a CD11b+ isolation kit per the manufacturer’s recommended protocol (Miltenyi Biotec, Auburn, CA; cat# 130-091-100). Prior to setting the culture ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were successively filtered through nylon filters (100 µm and 20 µm) followed by a percoll gradient and MACS sorting with CD45 Microbeads (Miltenyi Biotec). Cells were stained with APC anti-human EpCAM antibody (Biolegend ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were added at a dilution of 1:100 in autoMACs Rinsing Solution (Cat. No. 130-091-222, Miltenyi Biotec) containing 0.5% BSA at 4 °C for 1 hour ...
-
bioRxiv - Immunology 2022Quote: ... DCs were isolated from murine lymph nodes using magnetic beads according to the manufacturer's instructions (Miltenyi Biotec, Cat.No. 130-100-875). OT-1 T cells were isolated from peripheral blood of OT-1 mice and labeled with 0.5 μM CFSE (Thermo Fisher ...
-
bioRxiv - Immunology 2022Quote: ... for 20 minutes at 4 °C before being washed in flow cytometry buffer and re-suspended in 100 µl antibody staining cocktail (antibodies and FcR blocking reagent (Miltenyi Biotec) diluted as indicated above) ...
-
bioRxiv - Immunology 2023Quote: Monocytes were isolated from the bone marrow of Apoe-/-LysMCre and Apoe-/-LysMCreCnr1flox/flox mice by magnetic separation monocyte Isolation Kit (130-100-629, Miltenyi Biotec). The purified monocytes were labelled with carboxyfluorescein diacetate succinimidyl ester (CFSE ...
-
bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The complete volume of the resulting suspension was cleared by filtering through a 100 µm filter (Miltenyi Biotec, Bergisch Gladbach, Germany) which was washed once with 1× HBSS afterwards ...
-
bioRxiv - Molecular Biology 2023Quote: ... (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells, #130-092-657, Miltenyi Biotec for natural killer cells ...
-
bioRxiv - Immunology 2024Quote: ... Purified CD154+ T cells were expanded in presence of 1:100 autologous antigen-loaded irradiated feeder cells in TexMACS medium (Miltenyi Biotec), supplemented with 5% (v/v ...
-
bioRxiv - Immunology 2023Quote: ... Human blood samples were enriched for T cells using negative bead selection (StemCell) and stimulated in vitro in media containing a 1:100 titer of TransAct (Miltenyi Biotec), 5% FBS and 100 IU/mL of rhIL-2 ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were isolated and homogenized in STKM buffer (250 mM Sucrose, 50 mM Tris-HCl, 25 mM KCl, 5 mM MgCl2) using the GentleMACS (Miltenyi Biotec) program Protein_01 ...
-
bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...