Labshake search
Citations for Miltenyi Biotec :
551 - 600 of 667 citations for Methylphosphonic Acid 13C 99%; Methyl D3 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... The supernatant was removed and 1 mL of wash buffer (PBS + 0.5% BSA (Miltenyi Biotec, Cat. # 130-091-376) + 1 U/uL of RNase inhibitor ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then pelleted and resuspended in 5 mL PBSF and sorted using a MidiMACS Separator magnet (Miltenyi Biotec) in combination with an LS column (Miltenyi Biotec ...
-
bioRxiv - Plant Biology 2023Quote: ... 1.5 ml of extract was incubated with 50 µl of either Anti-Myc (Miltenyi Biotec, ref. 130-091-123) or Anti-HA (Miltenyi Biotec ...
-
bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
-
bioRxiv - Immunology 2022Quote: ... 5 mL of FACS buffer was added and the samples were passed over a magnetized LS column (Miltenyi Biotec). The tube and column were washed once with 5 mL FACS buffer and then removed from the magnetic field ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
-
bioRxiv - Immunology 2022Quote: ... 5 mL of FACS buffer was added and the samples were passed over a magnetized LS column (Miltenyi Biotec). The tube and column were washed once with 5 mL FACS buffer and then removed from the magnetic field ...
-
bioRxiv - Microbiology 2024Quote: ... in cell culture media supplemented with M-CSF (25 µg/mL, mouse M-CSF #130-101-704, Miltenyi Biotec) and antibiotics ...
-
bioRxiv - Immunology 2024Quote: ... PBMC were incubated at 37ºC for 15 minutes with 0.5 μg/mL anti-human CD40 blocking antibody (Miltenyi Biotec) per well ...
-
bioRxiv - Microbiology 2020Quote: ... antibody for 45 min at 4°C followed by the incubation with the secondary antibody anti-IgG1-PE (REA1017, Miltenyi Biotec, 1 μL/105 cells in 100 μL) for 30 min at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 minutes at 4°C in the dark ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell pellets were resuspended in 100 µl cold D-PBS and stained with fluorochrome-conjugated antibodies recognizing human CD11b and GFAP (Miltenyi Biotec, 130-113-810 and 130-123-846) as described above ...
-
bioRxiv - Neuroscience 2024Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA-2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 min at 4°C in the dark ...
-
bioRxiv - Immunology 2021Quote: ... Splenocytes or lung leukocytes were stimulated in duplicates with SARS-CoV-2 S peptide pools (2 μg/ml, Miltenyi Biotec) for 24 h at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... re-suspended in PBS/0.5% BSA buffer (5 × 106 cells/ml) and purified by magnetic cell sorting using anti-O4 MicroBeads (Miltenyi Biotec) following the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Spleens were collected and homogenized in PBS supplemented with 2.5 mg/ml saponine using the gentleMACS homogenizer with gentleMACS M tubes from Miltenyi Biotec. Amastigotes were purified as previously described [21] for DNA extraction ...
-
The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... and were allowed to differentiate into Mφs in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF, 20 ng/mL; Miltenyi Biotec) over a 6-day period ...
-
bioRxiv - Immunology 2024Quote: ... resuspended at 4 mio cells/ml and then cultured with HLA class I-enhanced neurons (see Human-iPSC-derived neuron preparation section above) at a density of 1.2mio cells/cm2 with IL-2 (1000UI/ml, Miltenyi Biotec) and a CD28 agonist antibody (5ug/ml ...
-
bioRxiv - Immunology 2024Quote: ... CD8+ L1CAM-CAR T cells (used for in vitro experiments) were generated and expanded as previously described (16) and supplied with 0.5ng/ml IL15 (Miltenyi Biotec) and 50U/ml IL2 (Novartis) ...
-
bioRxiv - Bioengineering 2022Quote: ... cells were washed in a 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
-
bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
-
bioRxiv - Molecular Biology 2023Quote: ... or tumor necrosis factor-alpha (1 ng/mL, TNF-α; catalog no. 130-094-022, Miltenyi Biotec, Bergisch Gladbach, Germany) in combination with LPS ...
-
bioRxiv - Genetics 2023Quote: ... and the precipitated pellet was resuspended in 2.5 mL 1× Buffer and filtered with a 70 µM MACS SmartStrainer (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Molecular Biology 2024Quote: ... MDSL and TF-1 were additionally supplemented with 25ng/mL of recombinant human IL-3 (Miltenyi Biotec, 130-095-069), and MDS-L with 50nM β-mercaptoethanol (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were washed three times and incubated in PBS containing DAPI (1 μg/mL) for 15 min before samples were processed using flow cytometry (MACSQuant, Miltenyi Biotec). At least 10000 events were analyzed per sample using FlowLogic software (Miltenyi Biotec).
-
bioRxiv - Cell Biology 2020Quote: ... was placed in MACS magnetic separator and the column was rinsed thrice with 1 mL rinsing solution (MACS BSA Stock Solution diluted 1:20 with autoMACS Rinsing Solution, Miltenyi Biotec). Beads bound to EVs were applied onto a magnetic column and Thy1-negative EVs that passed through the column were collected.
-
bioRxiv - Immunology 2019Quote: HIV-1-infected or mock-infected CD8-depleted PBMCs cells were used as targets and autologous cryo-preserved PBMCs rested overnight in R10 supplemented with 10ng/ml of IL-15 (Miltenyi Biotec) were used as a source of effector cells ...
-
bioRxiv - Cancer Biology 2019Quote: ... whole lungs from mice bearing MDA-LM2 macrometastases (3 weeks post tail vein injection) were harvested and lysed in 1 ml Cell Lysis Buffer 2 (R&D) using the gentleMACS Dissociator (Miltenyi Biotec). As a control ...
-
bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
-
bioRxiv - Neuroscience 2019Quote: ... or left untransduced for 4 days prior to lysis in mitochondria isolation kit lysis buffer (1×107 cells/mL; Miltenyi Biotec) with protease inhibitors (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
-
bioRxiv - Microbiology 2020Quote: ... One gram of each tissue was homogenized in 9 ml of PBS in gentleMACS M tubes using the appropriate setting on the gentleMACS dissociator (Miltenyi Biotec). Homogenates were filtered through 40-μm-pore-size filters and an aliquot was used to determine viable counts ...
-
bioRxiv - Microbiology 2021Quote: ... were obtained by 7 days stimulation with 20 ng ml−1 macrophage colony-stimulating factor (M-CSF) and 10 ng ml−1 granulocyte-macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec).
-
bioRxiv - Molecular Biology 2020Quote: ... dissolved in Hank’s balanced salt solution containing 50 μg/ml DNAse I and analyzed using a MACSQuant VYB flow cytometer (Miltenyi Biotec, Germany). Sytox Blue dead cell stain (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
-
bioRxiv - Immunology 2021Quote: ... The organs were homogenized in 2 ml PBS using gentleMACS M tubes and gentleMACS Octo Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Afterward ...
-
bioRxiv - Developmental Biology 2022Quote: ... The media was further supplemented with 5µM CHIR99021 (StemMACS, 130-103-926) on D2 and with or without 50 ng/ml hGdf11 (130-105-776, Miltenyi Biotec) on D3 ...
-
bioRxiv - Molecular Biology 2020Quote: PBMC were obtained from healthy donors and were cultured in 48-well plates at a density of 1 × 106/ml in TexMACS (Miltenyi Biotec), 3% human serum (Seralab ...
-
bioRxiv - Microbiology 2020Quote: ... Each section was weighed and then homogenized in 2 ml PBS in gentleMACS M tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec) using the RNA_1 setting ...
-
bioRxiv - Immunology 2021Quote: ... sorted B cells were cultured with rh IL-21 as previously described and with Human CD40-Ligand Multimer Kit (1 µg/mL, Miltenyi Biotec), rh IL-6 (20 ...
-
bioRxiv - Microbiology 2021Quote: ... Splenocytes (0.25 × 106) were stimulated in duplicates with SARS-CoV-2 S- or N-peptide pool (2 µg/ml, Miltenyi Biotec, USA) for 24 hours at 37°C ...
-
bioRxiv - Genetics 2019Quote: ... The flow-through was pelleted at 1250 rpm in a swinging bucket centrifuge set to 6°C then resuspended in 3 ml cold AutoMACs Running Buffer (AMB, Miltenyi Biotec). After gentle resuspension ...
-
bioRxiv - Immunology 2021Quote: ... The right lungs as well as the right hemispheres of the brains were homogenized in 1 ml PBS using a gentleMACS Octo Dissociator (Miltenyi Biotec) and viral RNA was isolated from 140 µl cleared homogenate or BAL fluid using QIAamp Viral RNA Mini Kit (Qiagen) ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then washed in PBS and cultured for 5 days in serum-free DMEM containing 50 ng/ml BDNF (Miltenyi Biotec Cat# 130-096-285 ...
-
bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
-
bioRxiv - Immunology 2021Quote: ... Samples were then transferred to 15 mL conical tubes and non-memory B cells were depleted using the AutoMACS Magnetic Sorter (Miltenyi Biotec) “deplete” program ...
-
bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.