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Citations for Miltenyi Biotec :
551 - 600 of 2297 citations for FabFc ZAP human Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (Miltenyi Biotec; Cat. No. 130-050-201) and LS Columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... following manufacturer instructions (Dead cell removal kit; Debris removal kit; Miltenyi Biotec) resulting in ~80% of purity and CD3+ T cells were resuspended in 1X PBS with 0.04% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... anti SSEA1 monoclonal antibody DSHB) and then incubated with a microbead-linked donkey anti-mouse IgM antibody (Miltenyi Biotec). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... Separation of mouse cells from human tumor cells was achieved using an LS column on a MACS Separator (Miltenyi Biotec). The flow-through fraction ...
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bioRxiv - Developmental Biology 2021Quote: ... the tip epithelial cells were isolated by EPCAM+ magnetic-activated cell sorting (MACS) beads according to the manufacturer’s instruction (CD326 MicroBeads, human, Miltenyi Biotec) from the distal lung tissues ...
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bioRxiv - Cell Biology 2021Quote: ... Human CD34+ cells were then enriched in the sample by immunomagnetic beads using an AutoMACSpro (Miltenyi Biotec, Bergisch Gladbach, Germany). After collection ...
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bioRxiv - Microbiology 2019Quote: ... 2 to 3×108 PBMCs were taken up in 2.1 ml staining buffer (PBS, 0.5 % BSA, 2 mM EDTA) and 300 μl of anti-human CD61 and CD3 microbeads (Miltenyi Biotec) were added to remove contaminating platelets and T cells ...
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bioRxiv - Immunology 2019Quote: ... The second PBMC portion was labelled with magnetic microbeads coated with a cross-reactive anti-human CD14 mAb to isolate monocytes per the manufacturer’s instructions (Miltenyi Biotec) (14) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Multiple myeloma cells were then isolated from WBCs using human CD138 affinity magnetic beads (Miltenyi Biotec; cat#130-093-062) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: Freshly collected human decidual tissues were trimmed into 1 mm3 piece by GentelMACS Dissociator (Miltenyi Biotec, 130-093-235, Germany) and digested twice for 30 minutes each at 37°C with 1.0 mg/ml type IV collagenase (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then resuspended in wash buffer and incubated in MACS human FcR blocking solution (20μl/sample, cat nb. 130-059-901, Miltenyi Biotec) and primary antibodies against CD45-FITC (12μl/sample ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in MACS buffer (PBS containing 2 mM ETDA and 2.5% FBS) supplemented with human FcR Blocking Reagent (Miltenyi Biotec) at 1:5 dilution and anti-human CD45-APC (BioLegend 304012 ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were resuspended in binding buffer after centrifuged and sorted via human CD3 MicroBeads (Miltenyi Biotec, #130-050-101) according to the manufacturer’s recommendation (Note ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Genomics 2023Quote: Human induced pluripotent stem cell lines were routinely cultured on Matrigel-coated dishes in MACS iPS-Brew media (Miltenyi Biotec) and passaged using ReLESR (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Bioengineering 2024Quote: ... All samples were blocked for non-specific binding with FcR Blocking Reagent for human (Miltenyi Biotec, cat.: 130-059-901) and mouse (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in MACS buffer (PBS, 0.5% BSA, 2 mM EDTA) for CD34+ magnetic selection using CD34 human MicroBeads (Miltenyi Biotec; cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 30 min and stained with 2μl of human EpCAM (FITC conjugated, Miltenyi Biotec, 130-080-301) and 1:75 of mouse MHC class I (APC conjugated ...
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bioRxiv - Plant Biology 2020Quote: ... the μMACS c-myc Isolation Kit and μMACS HA Isolation Kit (Miltenyi Biotec) were used according to the instructions provided ...
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bioRxiv - Microbiology 2019Quote: ... or IFN-γ detection antibody (Miltenyi Biotec, Germany). After washing the cells ...
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bioRxiv - Bioengineering 2020Quote: ... tagged with a CD34-PE antibody (Miltenyi Biotec), and purified on a fluorescence-activated cell sorting (FACS ...
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bioRxiv - Neuroscience 2021Quote: ... The CD31-FITC antibody (Miltenyi Biotec, Auburn, CA) was added to the cell suspension at a dilution of 1:50 ...
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bioRxiv - Immunology 2022Quote: ... Then the following antibodies (all from Miltenyi Biotec) were used ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary CD133 antibody (Miltenyi Biotec, 130-090-422) was diluted 1:25 in Antibody Diluent with Background Reducing Components (Dako ...
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bioRxiv - Cancer Biology 2023Quote: ... and the following antibodies purchased from Miltenyi Biotec: mCD335 (REA815) ...
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bioRxiv - Bioengineering 2024Quote: ... and anti-CD28 antibodies (clone: CD28.2; Miltenyi Biotec). Forty-eight hours before transduction and on the day of transduction ...
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bioRxiv - Cell Biology 2020Quote: ... CD133 expression was detected by anti-CD133–phycoerythrin (AC133-PE) antibody or PE-conjugated mouse immunoglobulin (Ig)G1 isotype control antibody (Miltenyi Biotec). The expression of Sox2 was analyzed by PerCP-Cy 5.5 mouse anti-Sox2 or PerCP-Cy 5.5 mouse IgG1 isotype control (Becton Dickinson [BD]) ...
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bioRxiv - Immunology 2022Quote: ... for 20 minutes at 4 °C before being washed in flow cytometry buffer and re-suspended in 100 µl antibody staining cocktail (antibodies and FcR blocking reagent (Miltenyi Biotec) diluted as indicated above) ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were labeled with a rat anti-CPM antibody (in-house) and microbead-conjugated anti-rat immunoglobulin kappa light chain antibody (130-047-401, Miltenyi Biotec). This was followed by the isolation of CPM+ lung progenitor cells using an autoMACS Pro Separator (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: Mouse and human brain samples were collected and mechanically dissociated using gentleMACS dissociation following the manufacturer’s instructions (Miltenyi Biotec, Paris, France). Freshly isolated tumor tissues were suspended in 5ml DMEM immediately after being surgically removed and placed in a petri dish ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed with PBS 1X and stained for 30 minutes using CD38 PE (IB60) Mouse Anti-Human (Cat.#130-092-260, Miltenyi Biotec)/CD38 FITC Multiepitope (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Biochemistry 2019Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL) followed by CD14+ MACS magnetic bead separation (Miltenyi Biotec). The isolated monocytes were differentiated for 7 days in IMDM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: Human iPSC/ESCs (Table S1) were maintained on dishes coated with Matrigel in mTeSR1 or StemMACS iPS-Brew XF (Miltenyi Biotec) media with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2019Quote: ... Monocyte isolation was performed by gradient centrifugation on Percoll (Pharmacia, Uppsala, Sweden) following by magnetic-activated cell separation sorting using human CD14 Microbeads (Miltenyi Biotec). Freshly isolated CD14+ monocytes were cultured for 7-8 days to differentiate into macrophages in IMDM medium supplemented with 10% FCS ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
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bioRxiv - Microbiology 2020Quote: Primary human CD14+ monocytes were isolated from the peripheral blood mononuclear cells of healthy donors using anti-CD14 magnetic beads (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25- cells were then obtained by depletion of CD25+ T cells using CD25 positive selection (CD25 microbeads II, Human, Miltenyi Biotec) and collecting the unlabeled cells.
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bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
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bioRxiv - Immunology 2023Quote: ... For two donors the B cells were isolated using MACS cell sorting with anti-human CD19 microbeads and MS columns from Miltenyi Biotec, according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were magnetically labeled and separated according to the instructions of CD45 MicroBeads Human (order no. 130-045-801, Miltenyi Biotec). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then plated at 1-1.5×106 cells/ml with 50ng/ml human FLT3-ligand (Miltenyi Biotec; 130-096-480) and cultured for 7 days in RPMI-1640 Glutamax (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Day 12 CD34+ hematopoietic cells were purified via magnetic-activated cell sorting (MACS) using human CD34 microbeads (#130-046-702, Miltenyi Biotec). As previously reported [48] ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Pathology 2024Quote: ... and the cells were resuspended in 200 μl of flow cytometry buffer and incubated with 15 μl of human FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...