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Citations for Miltenyi Biotec :
551 - 600 of 652 citations for 2' 5' Dichloro 3 3 4 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the cells were resuspended in PBS containing 2% FCS and 2mM EDTA and stained with the extracellular mix using APC-Vio770 anti-CD14 (clone REA599) from Miltenyi Biotec and used at 1/100 ...
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bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
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bioRxiv - Immunology 2022Quote: ... lungs of SARS-CoV-2 infected mice were collected and homogenized with gentle MACS M tubes (Miltenyi Biotec, 130-093-236) in a gentle MACS dissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes and microglia in the cell suspension were removed by using anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
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bioRxiv - Immunology 2021Quote: ... were co-transferred with CD3+ cell-depleted autologous PBMCs (2 × 106) isolated using CD3 Microbeads and the LD Column (Miltenyi Biotec) into 5- to 7-week-old female mice ...
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bioRxiv - Immunology 2020Quote: ... Cells were then washed three times and cultured with 1 ng/mL rhIL-15 or 20 IU/mL rhIL-2 (Miltenyi Biotec). After 3 days ...
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bioRxiv - Microbiology 2021Quote: ... at a ratio of one bead per cell for 72 h and maintained in RPMI with 100 U/mL IL-2 (AIDS Reagents Program) and 100 ng/mL IL-7 (Miltenyi Biotec). Monocyte-derived-macrophages (MDMs ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 2 min at room temperature in the presence of red blood cell lysis buffer (Miltenyi Biotec S.I.) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and incubated in 2% FBS in PBS with anti-ROR1-APC antibody (1:25, #130-119-860, Miltenyi Biotec) on ice for 30 minutes ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... loaded with biotinylated TCR and then for 1-2 additional rounds with tetramers of TCR and anti-AlexaFluor647 microbeads (Miltenyi Biotec). TCR tetramers were created by adding TCR to tetrameric AlexaFluor647-conjugated streptavidin (produced in-house ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells and CD4+ T cells were isolated from splenocyte suspensions using MACS MicroBeads (CD8a [Ly-2] and CD4 [L3T4] [Miltenyi Biotec]) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells and CD4+ T cells were isolated from splenocyte suspensions using MACS MicroBeads (CD8a [Ly-2] and CD4 [L3T4] [Miltenyi Biotec]) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The organs were homogenized in 2 ml PBS using gentleMACS M tubes and gentleMACS Octo Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Afterward ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... A red blood cell lysis step was performed by incubating cells for 2 minutes with 1x Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were washed again and then cell viability was measured using the Nexcelom Cellometer K2 Fluorescent Viability Cell Counter and the ViaStain AOPI Staining Solution (Nexcelom) ...
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bioRxiv - Microbiology 2020Quote: ... Each section was weighed and then homogenized in 2 ml PBS in gentleMACS M tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec) using the RNA_1 setting ...
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bioRxiv - Physiology 2021Quote: ... The muscle was removed from the -80 °C freezer and transferred to -20 °C where it was minced into pieces of 1-2 mm before being transferred to gentleMACS M-tubes (Miltenyi Biotec) tubes kept on ice ...
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bioRxiv - Immunology 2020Quote: ... PBMCs from 48 healthy individuals (samples taken before the SARS-CoV-2 outbreak) were enriched for CD19+ cells with CD19-microbeads (Miltenyi Biotec). For each individual ...
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bioRxiv - Immunology 2022Quote: ... cells were washed with 2% FBS containing PBS and incubated with 1:20 FcR blocking reagent (Cat. #130-059-901, Miltenyi Biotec) in 2% PBS for 10 min at RT ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were incubated for 72 hours at 37°C with either anti-CD3/anti-CD28 beads (1:2 bead-to-cell ratio, Miltenyi Biotec) or 200ng/mL N-803 in the presence of 50µM raltegravir and 5µM saquinavir ...
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bioRxiv - Immunology 2022Quote: B cells were purified from the spleens of mice (aged 2–7 months of age) through negative selection with anti-CD43 magnetic beads (Miltenyi Biotec) and subsequently cultured in RPMI 1640 media (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... and 1:8 with CD8+ T cells enriched using mouse CD8 (Ly-2) Microbeads kit (Miltenyi Biotec Cat # 130-049-401) and labeled with CellTrace Violet (CTV ...
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bioRxiv - Immunology 2023Quote: ... Cells (5×106) were infected at 5000 TCID50 / 1×106 cells at 37 °C for 2 hours on a cell-rotator (Miltenyi Biotec). After infection ...
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bioRxiv - Neuroscience 2023Quote: ... for all male cell lines (FD-1, FD-2, Ctrl) and the StemMACS mRNA reprogramming kit (Miltenyi Biotec, Bergisch Gladbach, Germany) for the female Fabry cell line (FD-3;26) ...
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bioRxiv - Immunology 2023Quote: ... T cells were stimulated by the addition of 100 IU/mL IL-2 (Proleukin) and anti-CD3/CD28 stimulation using loaded MACSiBead particles (Miltenyi Biotec) in a proportion of 1:1 of beads to cell ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in CTL test media (Immunospot) and incubated at 37 °C for 2 hours with either no stimulation or S1 peptide simulation (Miltenyi Biotec) at a final concentration of 2 µg/mL before adding GolgiPlug to all samples for 4 additional hours ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C for total of 30 minutes (15 minutes, 2 times) with rotated using MACSmix Tube Rotator (Miltenyi Biotec). Between and after the two incubations ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... Sarcoma tissue was collected on ice and immediately cut into 2 mm3 segments and dissociated to a single-cell suspension using the Human Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... In-vitro CD4 T cell survival assays were performed in complete RPMI medium supplemented with recombinant murine IL-2 (100 ng/ml, Miltenyi Biotec) or IL-7 (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Pieces of approximately 1 cm2 were placed in 10 mL PBS containing 2% FBS and homogenized for 10min at room temperature using a gentleMACS dissociator (Miltenyi Biotec). Homogenized tissue was filtered through a 70 μm cell strainer and pooled ...
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bioRxiv - Neuroscience 2024Quote: ... Then the astrocytes or newborn neurons were isolated using Anti-ACSA-2 MicroBead Kit or Anti-PSA- NCAM MicroBead Kit (Miltenyi Biotec). Briefly to say ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 900 µl/107 of isolation buffer containing 0.5% Bovine Serum Albumin (BSA) and 2 mM EDTA in PBS and 10 µl/1x107 of mouse anti- Ly6G MicroBeads (Miltenyi Biotec) and incubated at 4°C for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng ml−1 mouse recombinant vascular endothelial growth factor and 20 ng ml−1 human recombinant fibroblast growth factor-2 (all from Miltenyi Biotec) as described 85 and plated on pre-coated (Collagen I (10 µg ml−1 Millipore) ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for immunomagnetic cell separation with EpCAM-conjugated beads (Miltenyi Biotec, Catalog No. 130-105-958, 1/4). After 20 min incubation at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were isolated from at least 5×106 PBMCs on the day of thaw by negative selection using the CD4+ T cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Oligodendrocyte precursor cells (OPCs) were isolated from 5–7-day old YACQ84 transgenic mice using magnetic cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany). Whole brains were dissected and homogenized using the Neural Tissue Dissociation Kit P (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Genetics 2022Quote: ... in a cell culture incubator (37 °C; 5% CO2) followed by dissociation in gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in a total of 8 ml DMEM media with 10% FBS using gentleMACS Dissociator Program A twice and seeded onto 15 cm tissue culture dishes and grown in DMEM with 10% FBS at 37 °C with 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were centrifuged and resuspended in a 5% fetal bovine serum in PBS (FBS/PBS) with CD133-PE (Miltenyi Biotec 130-080-801), CD44-FITC (BD Pharmingen 555478 ...
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bioRxiv - Neuroscience 2024Quote: E15.5 embryo brains were extracted and cortices were dissociated into single-cell suspension using Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628). Progenitors were magnetically selected and isolated using Anti-Prominin1 MicroBeads (Miltenyi Biotec ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...