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Citations for Miltenyi Biotec :
501 - 550 of 637 citations for Recombinant Human Fc Fragment Of LgG Receptor Transporter Alpha since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... The second PBMC portion was labelled with magnetic microbeads coated with a cross-reactive anti-human CD14 mAb to isolate monocytes per the manufacturer’s instructions (Miltenyi Biotec) (14) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Multiple myeloma cells were then isolated from WBCs using human CD138 affinity magnetic beads (Miltenyi Biotec; cat#130-093-062) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: Freshly collected human decidual tissues were trimmed into 1 mm3 piece by GentelMACS Dissociator (Miltenyi Biotec, 130-093-235, Germany) and digested twice for 30 minutes each at 37°C with 1.0 mg/ml type IV collagenase (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then resuspended in wash buffer and incubated in MACS human FcR blocking solution (20μl/sample, cat nb. 130-059-901, Miltenyi Biotec) and primary antibodies against CD45-FITC (12μl/sample ...
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bioRxiv - Immunology 2021Quote: ... Human naïve B cells were isolated using the MACSxpress Whole Blood B Cell Isolation Kit (Miltenyi Biotec, 130-098-190) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used immediately for scRNA-seq ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in MACS buffer (PBS containing 2 mM ETDA and 2.5% FBS) supplemented with human FcR Blocking Reagent (Miltenyi Biotec) at 1:5 dilution and anti-human CD45-APC (BioLegend 304012 ...
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bioRxiv - Genomics 2022Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used for scRNA-seq ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were resuspended in binding buffer after centrifuged and sorted via human CD3 MicroBeads (Miltenyi Biotec, #130-050-101) according to the manufacturer’s recommendation (Note ...
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bioRxiv - Immunology 2022Quote: Treg and non-Treg cells were enriched using MACS CD4+CD25+CD127dim/− Regulatory T cell isolation Kit human (Miltenyi Biotec) from PBMCs of healthy donors and plated (1 × 105 cells per well ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Genomics 2023Quote: Human induced pluripotent stem cell lines were routinely cultured on Matrigel-coated dishes in MACS iPS-Brew media (Miltenyi Biotec) and passaged using ReLESR (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Neuroscience 2023Quote: ... Monocytes were then isolated using 250 μL of MicroBeads-conjugated human CD14 antibodies (StraightFrom LRSC CD14 MicroBead Kit; Miltenyi Biotec) added to 40 mL of cell suspension ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in MACS buffer (PBS, 0.5% BSA, 2 mM EDTA) for CD34+ magnetic selection using CD34 human MicroBeads (Miltenyi Biotec; cat ...
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bioRxiv - Biochemistry 2023Quote: Human primary monocytes were isolated from healthy donors using the autoMACS Pan Monocyte Isolation Kit (Miltenyi Biotec, 130-096-537). Briefly ...
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bioRxiv - Genetics 2024Quote: ... The PBMCs were further processed to isolate human primary T cells using MACS based PAN-T isolation kits (Miltenyi Biotec). RPMI-1640 (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... All samples were blocked for non-specific binding with FcR Blocking Reagent for human (Miltenyi Biotec, cat.: 130-059-901) and mouse (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from PBMC using Human CD4+ T Cell Isolation Kits (Miltenyi Biotec, Inc., Bergisch Gladbach, Germany). Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 30 min and stained with 2μl of human EpCAM (FITC conjugated, Miltenyi Biotec, 130-080-301) and 1:75 of mouse MHC class I (APC conjugated ...
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bioRxiv - Immunology 2020Quote: ... Human CD4+ T-cells were isolated from peripheral blood lymphocytes using naïve CD4+ T-cell isolation kit from Miltenyi Biotec (Germany). The blood samples were obtained from Rheumatology Clinic at Saint Louis University with informed consent ...
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bioRxiv - Cell Biology 2019Quote: ... for no longer than 1 h before their enzymatical and mechanical dissociation with the Whole Skin Dissociation kit for human material (Miltenyi Biotec) and the Gentle MACS dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: Mouse and human brain samples were collected and mechanically dissociated using gentleMACS dissociation following the manufacturer’s instructions (Miltenyi Biotec, Paris, France). Freshly isolated tumor tissues were suspended in 5ml DMEM immediately after being surgically removed and placed in a petri dish ...
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bioRxiv - Cell Biology 2019Quote: ... the cells of one donor were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed with PBS 1X and stained for 30 minutes using CD38 PE (IB60) Mouse Anti-Human (Cat.#130-092-260, Miltenyi Biotec)/CD38 FITC Multiepitope (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Primary human T regulatory cells (Tregs) were isolated from PBMCs using CD4+ CD25+ CD127dim/- Regulatory T Cell Isolation Kit (Miltenyi Biotec). The isolated Treg cells were expanded in culture for 14 days with TexMACS medium (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... The CD8+ T cells were purified from PBMCs using negative selection bead binding (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec). The following compounds were used at the indicated concentrations ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533 ...
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bioRxiv - Cancer Biology 2021Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Cancer Biology 2022Quote: ... Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec, Bergisch Gladbach, Germany). Pairs of liver samples from the same experimental group were pooled and subjected to a second round of purification ...
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bioRxiv - Immunology 2022Quote: The tissue pieces were rapidly transferred into the gentleMACS C Tube containing Human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec, Auburn, CA) and used immediately for further differentiation into macrophages [25] ...
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bioRxiv - Biochemistry 2019Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL) followed by CD14+ MACS magnetic bead separation (Miltenyi Biotec). The isolated monocytes were differentiated for 7 days in IMDM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: Human iPSC/ESCs (Table S1) were maintained on dishes coated with Matrigel in mTeSR1 or StemMACS iPS-Brew XF (Miltenyi Biotec) media with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2019Quote: ... Monocyte isolation was performed by gradient centrifugation on Percoll (Pharmacia, Uppsala, Sweden) following by magnetic-activated cell separation sorting using human CD14 Microbeads (Miltenyi Biotec). Freshly isolated CD14+ monocytes were cultured for 7-8 days to differentiate into macrophages in IMDM medium supplemented with 10% FCS ...
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bioRxiv - Cancer Biology 2021Quote: ... CD34+ human hematopoietic progenitor cells were isolated from human fetal liver tissue (obtained from Advanced Bioscience Resources) using the CD34 MicroBead Kit (Miltenyi Biotec) following the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... sorted B cells were cultured with rh IL-21 as previously described and with Human CD40-Ligand Multimer Kit (1 µg/mL, Miltenyi Biotec), rh IL-6 (20 ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
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bioRxiv - Physiology 2019Quote: ... Naive CD4 T cells were isolated from PBMCs by negative selection using the human Naive CD4+ T Cell Isolation Kit II (Miltenyi Biotec). The isolation was >97% pure as assessed by flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... 1x105 HEK-293T cells were mixed at a 1:1 ratio in 96-well plates with primary CD4+ T lymphocytes freshly isolated from healthy donors by negative selection (CD4+ T-Cell Isolation Kit II, human, Miltenyi Biotec). Viral transfer was assessed after 24 hours of incubation at 37°C in permeabilized (FIX & PERM Cell Permeabilization kit ...