Labshake search
Citations for Miltenyi Biotec :
501 - 550 of 3163 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... All setups were stained with 5μL of human FcR blocking reagent (Miltenyi Biotec, USA) to mitigate unspecific binding and LIVE/DEAD blue stain (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
-
bioRxiv - Immunology 2024Quote: ... Human monocytes were negatively selected from PBMCs using the monocytes Isolation Kit (Miltenyi Biotec). Monocytes were then plated 0.15×106/well onto 96-well plate (for ELISA ...
-
bioRxiv - Cancer Biology 2024Quote: ... Thawed and fresh samples were dissociated using the Tumor Dissociation Kit (human, Miltenyi Biotec) and tough dissociation protocol on gentleMACS Dissociator (Miltenyi Biotec ...
-
bioRxiv - Immunology 2022Quote: ... supplemented with 100 ng/mL of recombinant human flt3 ligand (Miltenyi Biotec, Auburn, CA), stem cell factor (SCF ...
-
bioRxiv - Cancer Biology 2023Quote: ... Enzymes and reagents from the human tumor dissociation kit (Miltenyi Biotec: 130-095-929) were added to the tumor tissue along with 1.75 mL of DMEM ...
-
bioRxiv - Genomics 2024Quote: ... using the human specific CD235a+ glycophorin A MicroBeads (Miltenyi Biotec Inc, 130-050-501). Purified CD235a+ were evaluated for erythroid maturation and enucleation (Table S3) ...
-
bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes were isolated by MACS using the human CD14+ isolation kit (Miltenyi Biotec). CD14+ monocytes were differentiated into DCs over 6 days by culturing in RPMI (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2019Quote: ... Primary monocytes were magnetically sorted by negative isolation per the manufacturer’s specifications (Miltenyi Biotec, Somerville, Massachusetts) and cultured in RPMI 1640 (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue ...
-
bioRxiv - Bioengineering 2023Quote: ... Conjugated primary antibodies used was anti-cardiac troponin 2 (TNNT2) PE (Miltenyi Biotec – 130-120-405). Viability marker Zombie Aqua (Biolegend – 423102 ...
-
bioRxiv - Immunology 2024Quote: Primary F4/80+ macrophages were isolated from WT mice following the manufacturer’s instructions (Miltenyi Biotec, Germany). Primary aortic smooth muscle cells (SMCs ...
-
bioRxiv - Cell Biology 2021Quote: Human neutrophils were isolated on a MACS LS column system (Miltenyi Biotec #130-042-401), enriched with magnetic anti-CD15 antibodies (Miltenyi Biotec #130-046-601 ...
-
bioRxiv - Cancer Biology 2020Quote: ... and anti-human CD38 (IB6, PE, Miltenyi Biotec, Cat No 130-113-989, 1:50) antibodies ...
-
bioRxiv - Bioengineering 2022Quote: ... Human monocytes were isolated from PBMC using a magnetic-based monocyte isolation kit (Miltenyi Biotec) and differentiated into macrophages with GMCSF (50 ng/mL ...
-
bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
-
bioRxiv - Systems Biology 2020Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (MACS Miltenyi Biotec) and LS Columns (Miltenybiotec) ...
-
bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...
-
bioRxiv - Immunology 2019Quote: ... density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201, Miltenyi Biotec). Monocytes were incubated for 7 days with RPMI-1640 (Gibco ...
-
bioRxiv - Cancer Biology 2021Quote: ... HDMECs were then isolated using a human CD31 MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... monocytes were purified from human PBMC by using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
-
bioRxiv - Genomics 2020Quote: ... anti-human EPCAM antibody conjugated to PE (1:50 dilution, Miltenyi Biotec #130-091-253), and either Calcein-AM (1:200 dilution ...
-
bioRxiv - Neuroscience 2022Quote: ... Microglia and myelin debris were subsequently removed through a mouse anti-CD45 (Invitrogen)-coated dish and myelin removal beads (Myelin Removal Beads II, human, mouse, rat, Miltenyi Biotec), respectively ...
-
bioRxiv - Cancer Biology 2024Quote: ... The freshly isolated PBMCs were subsequently cultured in human NK MACS® medium (Miltenyi Biotec) for 2 to 3 weeks to ensure optimal in vitro expansion of NK cells ...
-
bioRxiv - Cell Biology 2023Quote: ... CD14+ monocytes were then obtained by positive magnetic isolation using human CD14 microbeads (Miltenyi Biotec) and a MACS LS column with a QuadroMacs™ separator (Miltenyi Biotech) ...
-
bioRxiv - Cancer Biology 2023Quote: ... human according to manufacturer instructions and the GentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec) using custom settings ...
-
bioRxiv - Cancer Biology 2024Quote: ... and cultured in cRPMI supplemented with 5,000 U/ml recombinant human IL-2 (Miltenyi Biotec). Mouse NK (mNK ...
-
bioRxiv - Developmental Biology 2024Quote: A truncated human CD4 sequence (corresponding to the sequence identified by hCD4 MicroBeads, Miltenyi Biotec) was cloned into a pCS2+ vector containing an SP6 promoter ...
-
bioRxiv - Cancer Biology 2024Quote: ... and digested using the Tumor Dissociation Kit for human tissue (Miltenyi Biotec: #130-095-929) on a gentle MACS Octo Dissociator with heaters (Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2020Quote: ... and primary antibodies against CD45-FITC (12μl/sample, Cat nb. 130-114-567, Clone 5B1, Miltenyi Biotec), CD31-FITC (4μl/sample ...
-
bioRxiv - Plant Biology 2024Quote: ... 4% BSA) and incubated in a primary antibody solution anti-GFP-HRP (1/5000, MACS Miltenyi Biotec) and 1/5000 anti-His (11667475001 ...
-
bioRxiv - Neuroscience 2023Quote: Primary astrocytes were isolated from P0/P1 rat pup cerebral cortices using vendor established protocols (Miltenyi Biotec) and seeded on coated plates (30,000 cells/well in 96 well or 1 million cells/well in 6-well) ...
-
bioRxiv - Biochemistry 2019Quote: ... and subsequent CD14+ positive selection using the MACS Miltenyi Biotec Human CD14 microbead protocol (Miltenyi Biotec). CD14+ cells were differentiated in to macrophages using recombinant human granulocyte-macrophage colony-stimulating factor (200 ng/mL GM-CSF ...
-
bioRxiv - Cell Biology 2021Quote: ... and 60 ng/mL of human receptor activator of NF-κB-ligand (RANK-L, Miltenyi Biotec). The medium was then changed every third day by RPMI containing 10% FCS ...
-
bioRxiv - Cell Biology 2020Quote: ... or 20 ng/ml PDGF-AA (human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec). Inhibition of PDGF-signaling was performed by addition of 1μM imatinib (Cat nb ...
-
bioRxiv - Microbiology 2022Quote: ... Bacteria were stained with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 min on ice ...
-
bioRxiv - Immunology 2022Quote: Breast tissue was enzymatically and mechanically dissociated as per the Human Tumor Dissociation Kit (Miltenyi Biotec) protocol ...
-
bioRxiv - Cancer Biology 2019Quote: ... we also used CD3-PE to specifically identify lymphocytes (human antibody, clone BW264/56, Miltenyi Biotec). For all the samples ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and the media was further supplemented with 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2020Quote: For cultured human lung macrophages staining: anti-CD169 (7-239) and anti-CD11c (REA618, Miltenyi Biotec) were used for membrane staining ...
-
bioRxiv - Immunology 2021Quote: ... CD3 selection was performed after being transduced with γδTCR-Cl5 using human CD3 microbeads (Miltenyi Biotec). Moreover ...
-
bioRxiv - Immunology 2020Quote: ... Immature human moDCs were generated from PBMCs using CD14 Microbeads (Miltenyi Biotec cat. 130-050-201) and cultured for 6 days with recombinant GM-CSF (R&D Systems cat ...
-
bioRxiv - Microbiology 2022Quote: ... and human granulocyte-macrophage colony-stimulating factor (GM-CSF; 20ng/ml; MILTENYI BIOTEC; 130-093-866). At day 5 ...
-
bioRxiv - Developmental Biology 2024Quote: ... labeled with DLL1 anti-human antibody (APC-Vio 770, #130-106-148, Miltenyi Biotec, Bergisch Gladbach), or labeled with its associated isotype control (APC-Vio 770 ...
-
bioRxiv - Molecular Biology 2020Quote: Primary cardiac cultures were isolated from P7 mice using a neonatal dissociation kit (Miltenyi Biotec,130-098-373) using the gentleMACS homogenizer ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary microglia purity was examined by flow cytometry after staining by Cd11b-FITC (130-113-243, Miltenyi Biotec) and Cd45-APC (130-110-660 ...
-
bioRxiv - Physiology 2022Quote: Primary cardiac fibroblasts were isolated from 2-day-old rats using a neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... CD14+ monocytes were isolated from PBMCs using human anti-CD14 magnetic beads with LS Columns (Miltenyi Biotec). CD14+ monocytes were differentiated into MDM in RPMI 1640 medium supplemented with 10% FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... freshly isolated tumor samples were dissociated using the human tumor dissociation kit (Miltenyi Biotec; 130-095-929): surgical resection tissue was first minced into small pieces using a scalpel ...
-
bioRxiv - Cancer Biology 2022Quote: ... 25 μL Enzyme A (all provided in the human tumor dissociation kit (Miltenyi Biotec; 130-095-929), and 4.7 mL DMEM (Gibco ...