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Citations for Miltenyi Biotec :
501 - 550 of 1941 citations for Human SKI Like Oncogene SKIL CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... B cells were first isolated by magnetic cell sorting using the human CD19 MicroBeads (Miltenyi Biotec, San Diego, CA) and the AutoMACS magnetic cell separator ...
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bioRxiv - Cell Biology 2023Quote: ... Human CD34+ cells were enriched as previously described on a CliniMACS Prodigy according to the manufacturer’s instructions (Miltenyi Biotec). Nonhuman primate CD34+ cells were harvested ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were filtered to remove dead cells and B-cell depletion was performed using human CD19 microbead (Miltenyi Biotec) labeling according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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bioRxiv - Cancer Biology 2023Quote: ... CD138+ cells were isolated from bone marrow aspirate samples using Human CD138 MicroBeads (Miltenyi Biotec, Cat # 130-051-301). Selected cells were either viably cryopreserved in 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Developmental Biology 2023Quote: ... MNCs were resuspended in ice cold CliniMACS buffer supplemented with 0.5% human albumin or AutoMACS running buffer then labelled with anti-CD34 microbeads according to manufacturer’s instructions (Miltenyi Biotec). CD34+ cells were enriched using either a CliniMACS Plus (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Bioengineering 2024Quote: All samples were blocked for non-specific binding with human FcR Blocking Reagent (Miltenyi Biotec, cat.: 130-059-901) for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... The human iPSCs were grown on vitronectin and treated with small molecules LDN 193189 and A83-01 (Miltenyi Biotec) to induce a neural cell fate as well as with cyclopamine (Stem cell ...
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bioRxiv - Genomics 2024Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (Miltenyi Biotec; Cat. No. 130-050-201) and LS Columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... following manufacturer instructions (Dead cell removal kit; Debris removal kit; Miltenyi Biotec) resulting in ~80% of purity and CD3+ T cells were resuspended in 1X PBS with 0.04% BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... Separation of mouse cells from human tumor cells was achieved using an LS column on a MACS Separator (Miltenyi Biotec). The flow-through fraction ...
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bioRxiv - Developmental Biology 2021Quote: ... the tip epithelial cells were isolated by EPCAM+ magnetic-activated cell sorting (MACS) beads according to the manufacturer’s instruction (CD326 MicroBeads, human, Miltenyi Biotec) from the distal lung tissues ...
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bioRxiv - Cell Biology 2021Quote: ... Human CD34+ cells were then enriched in the sample by immunomagnetic beads using an AutoMACSpro (Miltenyi Biotec, Bergisch Gladbach, Germany). After collection ...
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bioRxiv - Microbiology 2019Quote: ... 2 to 3×108 PBMCs were taken up in 2.1 ml staining buffer (PBS, 0.5 % BSA, 2 mM EDTA) and 300 μl of anti-human CD61 and CD3 microbeads (Miltenyi Biotec) were added to remove contaminating platelets and T cells ...
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bioRxiv - Immunology 2019Quote: ... The second PBMC portion was labelled with magnetic microbeads coated with a cross-reactive anti-human CD14 mAb to isolate monocytes per the manufacturer’s instructions (Miltenyi Biotec) (14) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Multiple myeloma cells were then isolated from WBCs using human CD138 affinity magnetic beads (Miltenyi Biotec; cat#130-093-062) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: Freshly collected human decidual tissues were trimmed into 1 mm3 piece by GentelMACS Dissociator (Miltenyi Biotec, 130-093-235, Germany) and digested twice for 30 minutes each at 37°C with 1.0 mg/ml type IV collagenase (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then resuspended in wash buffer and incubated in MACS human FcR blocking solution (20μl/sample, cat nb. 130-059-901, Miltenyi Biotec) and primary antibodies against CD45-FITC (12μl/sample ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in MACS buffer (PBS containing 2 mM ETDA and 2.5% FBS) supplemented with human FcR Blocking Reagent (Miltenyi Biotec) at 1:5 dilution and anti-human CD45-APC (BioLegend 304012 ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were resuspended in binding buffer after centrifuged and sorted via human CD3 MicroBeads (Miltenyi Biotec, #130-050-101) according to the manufacturer’s recommendation (Note ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Genomics 2023Quote: Human induced pluripotent stem cell lines were routinely cultured on Matrigel-coated dishes in MACS iPS-Brew media (Miltenyi Biotec) and passaged using ReLESR (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in MACS buffer (PBS, 0.5% BSA, 2 mM EDTA) for CD34+ magnetic selection using CD34 human MicroBeads (Miltenyi Biotec; cat ...
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bioRxiv - Bioengineering 2024Quote: ... All samples were blocked for non-specific binding with FcR Blocking Reagent for human (Miltenyi Biotec, cat.: 130-059-901) and mouse (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 30 min and stained with 2μl of human EpCAM (FITC conjugated, Miltenyi Biotec, 130-080-301) and 1:75 of mouse MHC class I (APC conjugated ...
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bioRxiv - Plant Biology 2020Quote: ... the μMACS c-myc Isolation Kit and μMACS HA Isolation Kit (Miltenyi Biotec) were used according to the instructions provided ...
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bioRxiv - Cell Biology 2020Quote: Mouse and human brain samples were collected and mechanically dissociated using gentleMACS dissociation following the manufacturer’s instructions (Miltenyi Biotec, Paris, France). Freshly isolated tumor tissues were suspended in 5ml DMEM immediately after being surgically removed and placed in a petri dish ...
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bioRxiv - Immunology 2019Quote: ... (at 1:20-1:100 dilutions depending on the antibody) in the presence of human Fc receptor blocking reagent (1:10) (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed with PBS 1X and stained for 30 minutes using CD38 PE (IB60) Mouse Anti-Human (Cat.#130-092-260, Miltenyi Biotec)/CD38 FITC Multiepitope (Cat ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec, Auburn, CA) and used immediately for further differentiation into macrophages [25] ...
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bioRxiv - Biochemistry 2019Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL) followed by CD14+ MACS magnetic bead separation (Miltenyi Biotec). The isolated monocytes were differentiated for 7 days in IMDM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: Human iPSC/ESCs (Table S1) were maintained on dishes coated with Matrigel in mTeSR1 or StemMACS iPS-Brew XF (Miltenyi Biotec) media with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2019Quote: ... Monocyte isolation was performed by gradient centrifugation on Percoll (Pharmacia, Uppsala, Sweden) following by magnetic-activated cell separation sorting using human CD14 Microbeads (Miltenyi Biotec). Freshly isolated CD14+ monocytes were cultured for 7-8 days to differentiate into macrophages in IMDM medium supplemented with 10% FCS ...
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: ... Surgery-induced changes on monocyte surface antigen expression were analysed for HLA-DR (anti-human HLA-DR, clone REA805, Miltenyi Biotec) and CD62L ...
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bioRxiv - Microbiology 2020Quote: Primary human CD14+ monocytes were isolated from the peripheral blood mononuclear cells of healthy donors using anti-CD14 magnetic beads (Miltenyi Biotec) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25- cells were then obtained by depletion of CD25+ T cells using CD25 positive selection (CD25 microbeads II, Human, Miltenyi Biotec) and collecting the unlabeled cells.
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bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
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bioRxiv - Immunology 2023Quote: ... Cells were magnetically labeled and separated according to the instructions of CD45 MicroBeads Human (order no. 130-045-801, Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2023Quote: ... For two donors the B cells were isolated using MACS cell sorting with anti-human CD19 microbeads and MS columns from Miltenyi Biotec, according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then plated at 1-1.5×106 cells/ml with 50ng/ml human FLT3-ligand (Miltenyi Biotec; 130-096-480) and cultured for 7 days in RPMI-1640 Glutamax (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Day 12 CD34+ hematopoietic cells were purified via magnetic-activated cell sorting (MACS) using human CD34 microbeads (#130-046-702, Miltenyi Biotec). As previously reported [48] ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched using human anti-CD8 MicroBeads and the automated magnetic activated cell sorting (autoMACS) system (both Miltenyi Biotec) according to the manufacturer’s instructions ...