Labshake search
Citations for Miltenyi Biotec :
501 - 550 of 560 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
-
bioRxiv - Immunology 2020Quote: ... The adherent monocytes were then washed twice with PBS and resuspended in RPMI supplemented with the cytokines GM-CSF (1000 U/mL) and IL-4 (500 U/mL) (Miltenyi Biotec). Differentiation to immature MDDCs was achieved by incubation at 37 °C with 5% CO2 for 7 days and subsequent activation with cytokines addition every other day ...
-
bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...
-
bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
-
bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
-
bioRxiv - Genomics 2022Quote: ... Cells were then stained with the following antibodies for 30 minutes at 4°C: CD3 VioGreen (clone BW264/56, Miltenyi Biotec), CD14 V500 (clone M5E2 ...
-
bioRxiv - Immunology 2022Quote: ... Monocytes were cultured with 100 ng/mL (500 UI/mL) GM-CSF plus 50 ng/mL (250 UI/mL) IL-4 (Miltenyi Biotec) at 106cells/mL in complete RPMI medium supplemented with 10% FCS (Gibco) ...
-
bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
-
bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
-
bioRxiv - Neuroscience 2024Quote: ... The resulting cell suspension was spun down for 5 min at 300 × g and 4°C and processed for MACS using the CD11b/c microbeads according to the manufacturers protocol (Miltenyi Biotec). Subsequently ...
-
bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... cells were collected and centrifuged at 500 x g for 5 min at 4°C and CD8 T cells were isolated by magnetic sorting according to the manufacturer’s protocol (Miltenyi Biotec, Germany). To select transduced CD8 T cells ...
-
bioRxiv - Neuroscience 2024Quote: ... for 10 min at 4°C and incubated with anti-mouse CD11b-PE (1:50 dilution; 130-113-806, Miltenyi Biotec) and anti-mouse CD45-APC (1.15:100 dilution ...
-
bioRxiv - Microbiology 2024Quote: ... Harvested cells were centrifuged at 300×g for 5 min at 4°C and resuspended in red blood cell lysis buffer (#130-094-183, Miltenyi Biotec) for 5 min ...
-
bioRxiv - Immunology 2024Quote: ... 4 days later peritoneal washes were collected and F4/80+ macrophages were sorted with magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) with the typical purity yield >95%.
-
bioRxiv - Immunology 2024Quote: ... PBMCs were isolated using Ficoll-Hypaque density centrifugation and pDCs were positively selected from PBMCs using BDCA-4-magnetic beads (MACS Miltenyi Biotec), as previously described (Decembre et al. ...
-
bioRxiv - Neuroscience 2024Quote: ... 2 to 8 tubes (4 to 16 slices) of frozen tissue were dissociated using GentleMACS nuclei isolation protocol (nuclei isolation buffer [Miltenyi Biotec, cat# 130-128-024] ...
-
bioRxiv - Immunology 2024Quote: ... Intracellular staining was carried out for 30 min in the dark at room temperature with anti-4-1BB-PE (Miltenyi Biotec), anti-CD40L- PEVio770 (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2024Quote: ... CD8 T cells from autologous blood and tonsils from 4 HLA-A*02:01 donors were enriched using negative selection magnetic beads (CD4, CD19 depletion, Miltenyi Biotec). Roughly 1.5 e6 enriched CD8s were stained with Fc block for 10 minutes on ice ...
-
bioRxiv - Bioengineering 2024Quote: ... frozen liver samples were added to ice-cold PBS at 50 mg/mL and processed for tissue homogenization using the gentleMACS Dissociator (n = 3 per group) (Miltenyi Biotec, Bergisch Gladbach, Germany). Then ...
-
bioRxiv - Cell Biology 2024Quote: ... the pellet from each BM sample was resuspended in 392µl PBS/3% FBS and 8µl CD117 (cKit) MicroBeads (Miltenyi Biotec, catalog number 130-091-224) and incubated on ice for 30 mins ...
-
bioRxiv - Immunology 2021Quote: ... Cytokines measured from PBMC supernatants were collected after 4 days of incubation with S protein and concentrations were predicted using a standard curve in the LEGENDplex assay (Miltenyi Biotec 741028).
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in PBS with 0.5% of Bovine Serum Albumin (BSA, pH 7.2) and incubated for 15 min at 4 °C with myelin removal beads II (Miltenyi Biotec, Cologne, Germany) for myelin and debris removal ...
-
bioRxiv - Microbiology 2023Quote: ... Neutrophils were isolated from 4 different donors using the MACSxpress Neutrophil Isolation and MACSxpress Erythrocyte Depletion kits (Miltenyi Biotec Inc., Auburn, CA) and suspended in RPMI 1640 (without phenol red ...
-
bioRxiv - Neuroscience 2024Quote: Single cell suspensions of 1323-4 hiPSC-derived organoids were prepared with neural tissue dissociation kits (P) (Miltenyi Biotec, 130-092-628) following manufactural instructions ...
-
bioRxiv - Immunology 2024Quote: ... the absence of exogenous Th17- promoting cytokines (ii) with 10ng/ml of human recombinant polarizing cytokines (i.e.,TGF-β, IL-4, IL-12; Miltenyi biotec, Germany) or WNT10a ...
-
bioRxiv - Immunology 2024Quote: ... anti-CD14 and anti-BDCA-4 microbeads and subjected to AutoMACS selection (possel_s program followed by possel) (Miltenyi Biotec, Bergisch-Gladbach, Germany). Purity of isolated cells and ratios (PDC/B cells ...
-
bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... liver and brain samples were homogenised in PBS (1-3 mL, adjusted to tissue weight) using gentleMACS™ M tubes (Miltenyi Biotec Ltd, Bisley, Surrey) and gentleMACS™ octo dissociator using the standard protein program ...
-
bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
-
bioRxiv - Immunology 2020Quote: ... and the remaining adherent monocytes were cultured for 48h in the presence of IL-4 (300 U/ml) and GM-CSF (450 U/ml) (both from Miltenyi Biotec, Madrid, Spain) in X-VIVO 15 (BioWhittaker ...
-
bioRxiv - Cancer Biology 2022Quote: Samples were processed immediately after surgery and temporarily stored for transport at 4°C in tissue storage solution (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously described ...
-
bioRxiv - Developmental Biology 2024Quote: ... and Dlk1+ hepatoblasts were labeled with anti-Dlk mAb-FITC (MBL; D187-4; Rat) and anti-FITC MicroBeads (Miltenyi Biotec; 130–048-701). Labeled Dlk + hepatoblasts were isolated using a magnetic column (Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2022Quote: ... for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2, Miltenyi Biotec, 130-117-701) or corresponding IgG1-APC isotype (clone IS5-21F5 ...
-
bioRxiv - Physiology 2024Quote: ... Lysates were centrifuged (16,000 g, 10 minutes, 4°C) and the supernatants passed through 70 µm pre-separation filters (Miltenyi Biotec, Bergisch Gladbach, Germany). A fraction (10% ...
-
bioRxiv - Plant Biology 2024Quote: ... membranes were probed overnight at 4°C with the corresponding antibodies : anti-GFP-conjugated with HRP (Miltenyi Biotec 130-091-833, 1:5000), anti-RFP (Abcam 34767 ...
-
bioRxiv - Neuroscience 2024Quote: ... Each cerebral hemisphere was then cut in 4 sagittal slides before being enzymatically and mechanically digested using a gentleMACSTM Dissociator (#130-093-235, Miltenyi Biotec, 37C_ABDK_01 program) and the Adult Brain Dissociation kit (#130-107-677 ...
-
bioRxiv - Immunology 2024Quote: PDC were depleted from PBMC by AutoMACS (deplete_s) after preincubation with Fc block and anti-CD304 microbeads (BDCA-4/Neuropilin-1) MicroBead kit (Miltenyi Biotec, Bergisch-Gladbach, Germany). Total PBMC controls were subject to the same AUTOMACS protocol ...
-
bioRxiv - Cell Biology 2021Quote: Different lineages of in vitro cultured endMSCs (n = 3) were primed using a combination of recombinant pro-inflammatory cytokines and Toll-like receptors (TLR) ligands: IFNγ (Miltenyi Biotec Inc, San Diego, CA, USA) at 1 ng/ml and 100 ng/ml ...
-
bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
-
bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The cells are centrifugated at 600 g for 7 min at 4 °C and resuspended with 180 μl running buffer (Miltenyi Biotec, 130-091-221) for MACS selection.
-
bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
-
bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
-
bioRxiv - Developmental Biology 2024Quote: ... centrifuged (200 g for 3 min at 4°C) and resuspended in a solution composed by 80% FACS buffer and 20% anti-rat IgG MicroBeads (Miltenyi Biotec, 130-048-501). Cells were incubated in the mixture for 15 min at 4°C ...