Labshake search
Citations for Miltenyi Biotec :
451 - 500 of 570 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... membranes were probed overnight at 4°C with the corresponding antibodies : anti-GFP-conjugated with HRP (Miltenyi Biotec 130-091-833, 1:5000), anti-RFP (Abcam 34767 ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 10 % heat-inactivated FCS and 50 ng/ml GM-CSF (130-093-862; Miltenyi Biotec). Cells were cultured at 37°C under a humidified 5% CO2 atmosphere for 6 days and the medium was replaced at day 3 ...
-
bioRxiv - Developmental Biology 2023Quote: ... then incubated with anti-Mouse IgG micro-bead (1:10 in autoMACS Running Buffer, Miltenyi Biotec, Germany) for 30 min at 4°C ...
-
bioRxiv - Genomics 2024Quote: PitNET samples were dissociated with 8-10 ul of enzyme D Multi Tissue Dissociation Kit (Miltenyi Biotec) or 2 mg/ml collagenase IV (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: 10 μl of anti-ACSA-2 MicroBeads (Anti-ACSA-2 MicroBead Kit, # 130-097-678, Miltenyi Biotec) and an additional incubation for 30 min ...
-
bioRxiv - Immunology 2024Quote: ... Samples were acquired using the MACSQuant Analyzer 10 or MACS Quant Analyzer VYB flow cytometers (Miltenyi Biotec) and analyzed using FlowJo 10.
-
bioRxiv - Bioengineering 2024Quote: ... before analysis by flow cytometry on a MACSQuant Analyzer 10 or 16 (Miltenyi Biotec, Bergisch Gladbach, Germany). Data from this experiment were analyzed using FlowJo 10.6.2 software (FlowJo ...
-
bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
-
bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
-
bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
-
bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
-
bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
-
bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
-
bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
-
bioRxiv - Developmental Biology 2024Quote: ... centrifuged (200 g for 3 min at 4°C) and resuspended in a solution composed by 80% FACS buffer and 20% anti-rat IgG MicroBeads (Miltenyi Biotec, 130-048-501). Cells were incubated in the mixture for 15 min at 4°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... cell cycle analysis was carried out by flow cytometry with a MACSQuant 10 or VYB cytometer (Miltenyi Biotec) and analysed with FlowLogic software.
-
bioRxiv - Neuroscience 2022Quote: ... MACS NeuroBrew-21 with retinoic acid with final concentrations noted (10 mL, MACS Miltenyi Biotec, 130-093-566), PD0332991 (20 µM ...
-
bioRxiv - Immunology 2022Quote: ... Acquisition of a minimum of 100,000 cells per sample was performed on a MACSQuant 10 analyzer (Miltenyi Biotec). Single-stained controls using mouse Ig/ κ compensation beads (BD Biosciences ...
-
bioRxiv - Bioengineering 2020Quote: ... resuspended in MACSPlex buffer and analyzed by flow cytometry with a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). FlowJo software (version 10.6.2 ...
-
bioRxiv - Immunology 2023Quote: ... PBMCs were activated with 1 µg/ml PHA in presence of 10 ng/ml IL-2 (Miltenyi Biotec). PBMCs were mock- treated or infected with multiplicity of infection (MOI ...
-
bioRxiv - Neuroscience 2022Quote: ... and 1:10 50nm-magnetic-bead antibody solution against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523). Nerve fibroblasts express the marker CD90.1 ...
-
bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
-
bioRxiv - Immunology 2022Quote: ... Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec, catalog# 130-042-201).
-
bioRxiv - Cancer Biology 2020Quote: Dorsal skin was removed from euthanized 4-week-old C57BL/6 mice and dissociated with the Epidermis Dissociation Kit (Miltenyi Biotec, Cat. 130-095-928) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
-
bioRxiv - Developmental Biology 2020Quote: ... Disaggregated blastomeres in solution were incubated with 10 μl of magnetic microbeads conjugated to mouse anti-FITC (Miltenyi Biotec) for 15 min on ice ...
-
bioRxiv - Immunology 2021Quote: Tumors were dissociated in 3ml of RPMI with 10% FBS using the m_imptumor_01 setting on a gentleMACS dissociator (Miltenyi Biotec). Samples were transferred to a conical tube with 35mL of Collagenase (RPMI containing 1% HG solution ...
-
bioRxiv - Immunology 2020Quote: ... PBMCs or purified NK cells were stimulated for 16-18 hours with 10 ng/mL rhIL-12 (Miltenyi Biotec), 100 ng/mL rhIL-15 (Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2023Quote: ... Analyses of the labeled cells were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). The proportion of eGFP+ cells that co-labelled with CD11b+CD45+ was assessed and compared between groups using MACSQuantify v2.13.0 software.
-
bioRxiv - Immunology 2023Quote: We prepared B cell tetramers of biotinylated S2hlx-Ex19 or FP-10 by mixing with Streptavidin-VB515 (Miltenyi Biotec) and Streptavidin-AlexaFluor 647 (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were incubated for 10 min with 25 µl of FcR Blocking Reagent (Miltenyi Biotec 130-059-901) (pre-diluted 1:50 with FACS buffer (PBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
-
bioRxiv - Immunology 2024Quote: ... Samples were digested (100µg/ml DNase and 0.4 mg/ml collagenase in 10% FBS/RPMI) using GentleMacs Dissociator (Miltenyi biotec), followed by filtering with 70μm strainer using 10% FBS/RPMI ...
-
bioRxiv - Pathology 2024Quote: Detergent-extracted brain homogenates [10% (w/v)] were prepared in PBS using a gentle-MACS Octo Dissociator (Miltenyi BioTec). Nine volumes of brain homogenate were then mixed with one volume of 10× detergent buffer [5% (v/v ...
-
bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for immunomagnetic cell separation with EpCAM-conjugated beads (Miltenyi Biotec, Catalog No. 130-105-958, 1/4). After 20 min incubation at 4°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... and seeded at a density of 19,000 cells/cm2 on Matrigel in mTeSR1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec). Upon reaching 95% confluence ...
-
bioRxiv - Genomics 2020Quote: ... 3×108 cells were resuspended in 3 ml of FACS buffer (PBS with 10% FBS) and stained with 0.9 µg anti-ter119-PE (130-102-338; Miltenyi Biotec). Stained cells were conjugated to anti-PE microbeads (130-048-801 ...
-
bioRxiv - Genomics 2021Quote: ... BMMNC were either cryopreserved in FBS with 10% of DMSO or previously enriched for CD34+ (CD34 MicroBead Kit, Miltenyi Biotec) before cryopreservation.
-
bioRxiv - Neuroscience 2021Quote: Cells were pelleted at 300 x g for 10 minutes at 4ºC and resuspended in 90 µL of 0.5% BSA in D-PBS with 10 µL of CD11b (Microglia) MicroBeads (#130-093-636, Miltenyi Biotec) per 107 total cells ...
-
bioRxiv - Developmental Biology 2021Quote: ... The stroma was negatively selected against epithelium using a EPCAM antibody coated microbeads (1:10; Miltenyi Biotec #130-061-101) and a Miltenyi MS column.
-
bioRxiv - Immunology 2021Quote: ... 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells (Miltenyi Biotec, Cat#130-104-454) from Bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis at indicated time points ...
-
bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPLEX Cytokine 10 kit (Miltenyi Biotec). Also ...
-
bioRxiv - Immunology 2021Quote: ... the mouse cytokine profile was analyzed using the supernatant of the cultures using the MACSPlex Cytokine 10 kit (Miltenyi Biotec). Also ...
-
bioRxiv - Bioengineering 2022Quote: ... cells were washed in a 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed twice using PBS containing 0.05% BSA at 1,700 RPM for 5 minutes and processed in a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec, CA).
-
bioRxiv - Neuroscience 2023Quote: Hippocampal cells from C57BL6/N mice were pelleted at 300 x g for 10 minutes at 4°C then resuspended in 90 µL of 0.5% BSA in D-PBS and 10 µL of CD11b (Microglia) MicroBeads (#130- 093-634, Miltenyi Biotec) followed by thorough mixing ...
-
bioRxiv - Neuroscience 2023Quote: ... Hippocampal single cell suspensions were pelleted at 300 × g for 10□min at 4°C and resuspended in 90□μl of 0.5% BSA in D-PBS with 10□μl of CD11b (Microglia) MicroBeads (#130-093-636, Miltenyi Biotec) per 107 total cells ...
-
bioRxiv - Genomics 2023Quote: Singlet rate was estimated on dissociated tissues using flow cytometry on a MACSQuant Analyzer 10 (Miltenyi Biotec, 130-096-343). Flow cytometry data was analyzed using Flowlogic v8 (Miltenyi Biotec ...
-
bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in sorting buffer and incubated with ACSA-2 MicroBeads (1:10, Miltenyi Biotec, 130-097-678) for 15 min at 4°C ...