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Citations for Miltenyi Biotec :
451 - 500 of 778 citations for Testosterone 100 Ug Ml In Methylene Chloride 3 4 13C2 99%; 17 18O 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Monocytes were then enriched using a negative isolation protocol specific to mouse bone marrow (Miltenyi Biotec, 130-100-629), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... tissues were either freshly processed or transiently stored overnight MACS Tissue Storage solution (Cat. #130-100-008, Miltenyi Biotec) at 4 degree Celsius (in case of delayed surgeries ...
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bioRxiv - Synthetic Biology 2023Quote: ... Overnight cultures were diluted in 1:100 in DPBS and analyzed on a MACSQuant VYB flow cytometer (Miltenyi Biotec) using a previously described strategy to gate for single cells11 ...
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bioRxiv - Genomics 2024Quote: ... Biotinylated RNA was captured using 100 μl of streptavidin-coated μMACS magnetic beads (Miltenyi Biotec, Cat# 130-074-101) for 90 min at 24°C under gentle agitation ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in SFM with 10 ng/ml VEGF (Miltenyi Biotec), 10 ng/ml BMP4 (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... and 25 ng/ml macrophage colony-stimulating factor (M-CSF; Miltenyi Biotec). HeLa and HEK 293 FT cells were maintained by bi-weekly subpassage in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5ng/ml recombinant human GM-CSF (Miltenyi Biotec, 130-093-866). HEK293T cells (ATCC CRL-3216 ...
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bioRxiv - Microbiology 2021Quote: ... cultures were incubated in the presence of 20ng/ml IL7 (Miltenyi Biotec), 20ng/ml IL15 (Peprotech) ...
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bioRxiv - Developmental Biology 2022Quote: ... supplemented with 10 ng/ml hFGF-2 (Miltenyi Biotec, 130-104-925). The media was further supplemented with 5µM CHIR99021 (StemMACS ...
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bioRxiv - Immunology 2022Quote: ... 25ng/mL macrophage–colony stimulating factor (wt/vol, M-CSF, Miltenyi Biotec), and penicillin/streptomycin at 100U/0.1mg per mL of media respectively (wt/vol ...
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bioRxiv - Microbiology 2022Quote: ... was primed using 0.4 ml of MACSQuant Tyto Running Buffer (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... and 100IU/ml recombinant human IL-2 (Miltenyi Biotec, Bergisch Gladbach, Germany) were added ...
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bioRxiv - Microbiology 2023Quote: ... followed by IL-2 treatment (100U/mL Miltenyi Biotec 130-097-746). All experiments involving primary cells were performed at least 4 days post-activation.
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bioRxiv - Microbiology 2023Quote: ... with macrophage colony stimulating factor (M-CSF, 20 ng/mL; Miltenyi Biotec) (hereafter defined as complete medium) ...
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bioRxiv - Immunology 2023Quote: ... cells were blocked with 0.5μg/ml of anti-CD40 mAb (Miltenyi Biotec) for 15min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and/or human TNFα (10ng/ml, Miltenyi Biotec, CAT#130-095-015) in endothelial basal medium MV (EBM-MV ...
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bioRxiv - Genetics 2023Quote: ... supplemented with 1X 0.1mg/mL LIF 10000X (130-095-777, Miltenyi Biotec), 1X 5mM PD0325901 5000X (1408 ...
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bioRxiv - Cell Biology 2024Quote: ... plus interferon-γ (IFNγ; 20ng/mL; Miltenyi Biotec, Cat# 130-105-785) to induce inflammation (i.e. ...
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bioRxiv - Genomics 2024Quote: ... with 2 mL Nuclei Extraction Buffer (Miltenyi Biotec cat. #130-128-024) supplemented with 0.2 U/µL RNase Inhibitor (NEB cat ...
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bioRxiv - Microbiology 2024Quote: ... containing 1 mL PBSbefore homogenizationwith a tissue dissociator (Oligo-Macs, Miltenyi Biotec). The samples were serially diluted and plated on cetrimide agar.
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stored at 4°C in dark until the next morning for data acquisition using a MacQuant10 (Miltenyi Biotec, USA). Data was analyzed using FlowJo 10 (BD Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with the following antibodies for 30 minutes at 4°C: CD3 VioGreen (clone BW264/56, Miltenyi Biotec), CD14 V500 (clone M5E2 ...
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bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
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bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were collected and centrifuged at 500 x g for 5 min at 4°C and CD8 T cells were isolated by magnetic sorting according to the manufacturer’s protocol (Miltenyi Biotec, Germany). To select transduced CD8 T cells ...
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting cell suspension was spun down for 5 min at 300 × g and 4°C and processed for MACS using the CD11b/c microbeads according to the manufacturers protocol (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were incubated with the cells at 4°C for 30 minutes: anti-CD3 epsilon-VioBlue (Clone 17A2, Miltenyi Biotec), anti-TCR β-Alexa Fluor 488 (Clone H57-597 ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Genomics 2020Quote: ... CD34 positive cells were then bead enriched using the CD34 MicroBead Kit UltraPure (Miltenyi Biotec, catalog number 130-100-453) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse anti-Cy5/Alexa Fluor 647 (cocktail of antibodies at 1:100 each from Sigma-Aldrich [Cat# C1117, RRID:AB_477654] and Miltenyi Biotec [custom-ordered antibody used in their Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads ...
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bioRxiv - Immunology 2020Quote: ... Primary murine monocytes were isolated from C57BL/6J bone marrow using the Monocyte Isolation Kit (Miltenyi Biotec, 130-100-629) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
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bioRxiv - Immunology 2023Quote: ... then sieved via a 100 μm and 70 μm MACS® SmartStrainers (130-098-463, 130-098-462, Miltenyi Biotec) and 30 ml ice-cold 10% fetal FBS in RPMI-1640 were added to stop digestion ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Immunology 2021Quote: ... Cytokines measured from PBMC supernatants were collected after 4 days of incubation with S protein and concentrations were predicted using a standard curve in the LEGENDplex assay (Miltenyi Biotec 741028).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...