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Citations for Miltenyi Biotec :
451 - 500 of 709 citations for Recombinant Mouse PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were isolated by MACS using the mouse CD4+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 mg of tumor tissue was enzymatically and mechanically digested using the mouse Tumor Dissociation Kit (Miltenyi Biotec). For blood and spleens ...
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bioRxiv - Genomics 2024Quote: ... tissues from each mouse were transferred to a chilled gentleMACS C Tube (Miltenyi Biotec cat. #130-093-237) with 2 mL Nuclei Extraction Buffer (Miltenyi Biotec cat ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... APC rat anti-mouse ACSA-2 (cat # 130-117-535, Miltenyi Biotec, clone IH3-18A3, 1:25 dilution), AF 488 rat anti-mouse TMEM119 (cat # 53-6119-82 ...
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bioRxiv - Cell Biology 2023Quote: The brain (minus cerebellum and olfactory bulb) was dissociated using the adult mouse brain dissociation kit (Miltenyi Biotec). The tissue ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μl of 50nm-magnetic-bead antibodies against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523) and incubated for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: Whole mouse lungs were digested and single cell suspension was prepared using dissociation kit (Miltenyi Biotec, Auburn, CA) as per the vendor’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... GCB cells were first enriched from mouse splenocytes using the PNA MicroBead Kit (Miltenyi Biotec, 130-110-479), followed by antibody incubation and sorting on BD Aria II sorter (RRID:SCR_018091 ...
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bioRxiv - Physiology 2023Quote: Single-cell suspensions were prepared from mouse lungs using the Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: OPCs were isolated from p7 mouse brains using magnetic activated cell sorting and anti-NG2 MicroBeads (Miltenyi Biotec). Tissue dissection and cell sorting were performed under sterile conditions ...
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bioRxiv - Cancer Biology 2024Quote: The optically cleared mouse tissues were imaged using a light sheet fluorescence microscope (Ultramicroscope Super Plan, Miltenyi Biotec) equipped with a 5.5 Megapixel sCMOS camera (Andor Neo ...
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bioRxiv - Neuroscience 2024Quote: ... Aliquots (100 µL) were incubated with anti-mouse CD11b-APC (1:50 dilution; 130-113-802 Miltenyi Biotec) and anti-mouse CD45-PE (1:50 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... Half of the tissue was digested (as described above) using the Miltenyi mouse tumor dissociation kit (Miltenyi Biotec). The remaining tissue from each sample was embedded in OCT for H&E staining in order to classify the corresponding tumor phenotype ...
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bioRxiv - Immunology 2024Quote: ... MACS was performed according to the manufacturer’s instructions using Anti-Mouse IgG Microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were stored in RNAlater (Thermo Fisher Scientific Baltics UAB ...
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bioRxiv - Immunology 2024Quote: ... tumors and spleens underwent single-cell processing with a Mouse Tumor Dissociation Kit (#130-096-730; Miltenyi Biotec) and a Mouse Spleen Dissociation Kit (#130-095-929 ...
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bioRxiv - Immunology 2024Quote: ... and T cells were enriched via negative selection using the mouse Pan T cell Isolation Kit (Miltenyi Biotec). Cells were then expanded in vitro by cultured in RPMI 1640 (Gibco #11875093 ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitation (IP) was carried out with the μMACS isolation kits for tagged proteins from Miltenyi Biotec (Bergisch-Gladbach, Germany). The eluates ...
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bioRxiv - Molecular Biology 2020Quote: Immunoprecipitation of uL18L1-3HA and uL18L8-3HA were performed using the μMACS HA-Tagged Protein Isolation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Developmental Biology 2021Quote: ... GFP-tagged proteins were magnetically labelled and subsequent isolated using µMACS Isolation Kit (Miltenyi Biotec S.L., Bergisch Gladbach, Germany) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the complete sequence of B.1.1.7 D614G Spike mutant (alpha) (JPT Peptide Technologies) or wildtype (WT) S-protein (Miltenyi Biotec) or left untreated as a control for 16 h ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec #130-091-833), anti-plant actin mouse monoclonal antibody (3T3)-HRP (Abbkine # A01050HRP) ...
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bioRxiv - Molecular Biology 2023Quote: All datasets contained RNA and protein images and were analyzed in the MACS iQ View analysis software (Miltenyi Biotec). Every field of view was assessed across two exposure times during image preprocessing ...
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bioRxiv - Immunology 2021Quote: ... Bone marrows cells were purified with CD3+ T isolated kit (CD3ε MicroBead Kit, mouse, 130-094-973, Miltenyi Biotec). Splenic CD4+ and CD8+ cells were purified in two steps ...
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bioRxiv - Immunology 2022Quote: ... CD45+ cells (immune cells) and CD31+ (endothelial cells) were then depleted using mouse CD45 and CD31 MicroBeads (Miltenyi Biotec) and the remaining cells were counted and incubated with anti-CD326-APC (eBioscience) ...
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bioRxiv - Molecular Biology 2021Quote: Resting primary B lymphocytes (wild-type and Tp53bp1-/-) were purified from mouse spleens using anti-CD43 microbeads (Miltenyi Biotec). One million cells were stimulated to proliferate with a cytokine cocktail containing 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Developmental Biology 2020Quote: ... Disaggregated blastomeres in solution were incubated with 10 μl of magnetic microbeads conjugated to mouse anti-FITC (Miltenyi Biotec) for 15 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Microglia were isolated from adult mouse brain using the multi tissue dissociation kit 1 (Miltenyi Biotec #130-110-201), followed by CD11b microglia isolation kit (Miltenyi Biotec #130-093-634 ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens by using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Tumors and 1 cm2 of jejunum were cut into small pieces using the McIlwain™ Tissue Chopper and digested on the gentleMACS™ Octo Dissociator with Heaters (program, 37C_m_TDK_1) using the mouse Tumor Dissociation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Mouse CD4+ T cell isolation kit and CD8+ T cell isolation kit were obtained from Miltenyi Biotec (Auburn, CA). Clodronate and control liposomes were obtained from Liposoma (Amsterdam ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were purified from Cd226−/− 2D2 TCR transgenic mice by using mouse CD4 MicroBeads (Miltenyi Biotec). Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were sorted from pooled spleens of WT C57BL/6 mice by using mouse CD11c MicroBeads (Miltenyi Biotec). Then ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP [GG4-2C2.12.10] (1:5000; 130-091-833; Miltenyi Biotec), rabbit anti-EXOC1 antibody (1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... anti SSEA1 monoclonal antibody DSHB) and then incubated with a microbead-linked donkey anti-mouse IgM antibody (Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2021Quote: ... Cells were negatively enriched for CD8 cells using the Miltenyi Biotec mouse CD8+ T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2020Quote: ... cells were isolated from WT C57BL/6 spleens using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec) and cultured as described previously (Shakya et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Monocytes were then enriched using a negative isolation protocol specific to mouse bone marrow (Miltenyi Biotec, 130-100-629), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: Mature mouse and human retinal organoids were dissociated using the embryoid body dissociation kit (Miltenyi Biotec, 130-096-348) with the gentleMACS™ Dissociator according to the manufacturer’s automated dissociation protocol with a minor modification ...
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bioRxiv - Cell Biology 2024Quote: ... The wound tissues underwent enzymatic epidermal-dermal separation using the Epidermis Dissociation Kit (Epidermis Dissociation Kit, mouse; Miltenyi Biotec) followed by dissociation of the epidermis and dermis parts ...
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bioRxiv - Cell Biology 2023Quote: ... liver tissues were digested and dissociated on the gentleMACS Dissociator with the Mouse Liver Dissociation Kit (Miltenyi Biotec, 130105807). After digestion and dissociation ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions from tumor tissues were prepared using the Mouse Tumor Dissociation Kit (Miltenyi Biotec, Auburn, CA, USA) and Percoll density gradient separation according to the manufacturer’s recommendations (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... CD45.2+ splenic B cells from SWHEL donor mice were purified using anti-mouse CD43 (Ly-48) MicroBeads (Miltenyi Biotec). If not specified ...
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bioRxiv - Immunology 2023Quote: ... CD45.2+ donor splenocytes were enriched by CD45.1 negative selection using anti-mouse biotinylated CD45.1 antibody (clone A20, Ebioscience) and Anti-Biotin MicroBeads (Miltenyi Biotec).
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bioRxiv - Biophysics 2023Quote: ... and resting mouse B-cell isolation kit (Cat# 130-090-862) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Oligonucleotides were obtained from Integrative DNA Technologies (Coralville ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS/FCS and naïve CD4+ T cells were isolated using the naïve CD4+ T cell isolation kit (mouse) from Miltenyi Biotec according to their standard protocol ...
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bioRxiv - Immunology 2023Quote: ... Liver tissue was dissociated by following the ‘preparation of single cell suspensions from mouse liver’ protocol from Miltenyi Biotec. Cells were then washed in cold PBS+ 2% FBS buffer before staining ...
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bioRxiv - Physiology 2023Quote: ... fresh mouse lung tissues were collected and dissociated using a lung dissociation kit (Miltenyi Biotec Inc., Auburn, CA, USA). Lung ECs were then isolated using two step magnetic bead separation (CD45 and CD31 antibody- conjugated microbeads ...