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Citations for Miltenyi Biotec :
451 - 500 of 657 citations for Recombinant Human PDGFRB His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... pDCs were enriched from freshly isolated PBMCs using the human Plasmacytoid Dendritic Cell Isolation Kit II (Miltenyi Biotec) following manufacturer’s instructions with a 10% reduction in the recommended amounts of reagents ...
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bioRxiv - Synthetic Biology 2021Quote: ... and MACSiBead Particles pre-coated with anti-human CD335 (NKp46) and CD2 antibodies based on manufacturer guidelines (Miltenyi Biotec). Primary adult CD34+ HSCs were cultured at 0.5 x 106 cells per mL in SFEMII medium supplemented with CC110 cytokine cocktail (StemCell).
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bioRxiv - Cell Biology 2020Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec) and published protocols 50,51 ...
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bioRxiv - Immunology 2021Quote: ... Tumors were minced and processed following the instructions for the human tumor dissociation kit (Miltenyi Biotec, 130-095-929). After the addition of enzyme mix in 5 ml null RPMI ...
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bioRxiv - Molecular Biology 2020Quote: EV were depleted from citrated plasma using the exosome isolation kit pan human kit (130-110-912, Miltenyi Biotec) directed against a combination of CD9 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary human T cells were isolated from PBMCs by negative selection using the Pan T Isolation Kit (Miltenyi Biotec). Batch 1 T cells were cultured in IMDM Medium containing Icove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse CAFs were separated from human pancreatic UM2 cancer cells using the Mouse Cell Depletion Kit (MACS Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: EV samples were tested with a bead-based multiplex analysis (MACSPlex exosome kit, human, Miltenyi Biotec, Bergisch Galdbach, Germany). EV samples were diluted with MACSPlex buffer to a final volume of 120 µl with 5.8×108 EVs per sample ...
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bioRxiv - Microbiology 2022Quote: ... PE anti-human IgA-stained bacteria were incubated with Anti-PE Magnetic Activated Cell Sorting (MACS) beads (Miltenyi Biotec) (1:5 ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor and matched normal samples were dissociated into single cell suspension using human tumor dissociation kit (Miltenyi Biotec) and gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
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bioRxiv - Immunology 2019Quote: Single cell suspensions of PBMCs and CD15-depleted SF cells were blocked with human FcR blocking reagent (Miltenyi Biotec) and labeled for 15 min at 4 °C with antibodies for fluorescent activated cell sorting (FACS ...
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bioRxiv - Immunology 2019Quote: Human neutrophils were isolated by magnetic separation using MACSxpress whole blood neutrophil isolation kit (Miltenyi Biotec, 130-104-434) and were seeded into a micro-insert 4 well dish (Ibidi 80406) ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR-positive transduced T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Immunology 2020Quote: ... the cells were labeled with the IFN-γ Catch Reagent (IFN-γ Secretion Assay-Detection Kit, human Miltenyi Biotec) containing bispecific antibodies for CD45 and IFN-γ which was secreted by the stimulated target cells ...
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bioRxiv - Cancer Biology 2020Quote: ... The remaining tissue was further minced with scissors and enzymatically dissociated using the Human Tumour Dissociation Kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... The naïve T cells were purified from human PBMC using Pan T cell isolation kit (Miltenyi Biotec, Cat#130096535) and activated and expanded by CD3/CD28 Dynabeads (GibcoTM ...
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were purified using anti-human CD14 antibody-labeled magnetic beads and iron-based LS columns (Miltenyi Biotec) and used directly for further differentiation into macrophages (Dominguez-Soto et al. ...
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bioRxiv - Immunology 2020Quote: ... human peripheral blood mononuclear cells were activated with 50□ng/mL OKT3 (Cat. No. 130-093-387, Miltenyi Biotec) and harvested for retroviral transduction on day 2 ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Immunology 2020Quote: Primary human CD14+ monocytes were positively selected from PBMCs using CD14 Microbeads according to the manufacturer’s instructions (Miltenyi Biotec) and differentiated for 3 days with 20 ng/mL M-CSF ...
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bioRxiv - Immunology 2021Quote: ... B cells were first isolated by magnetic cell sorting using the human CD19 MicroBeads (Miltenyi Biotec, San Diego, CA) and the AutoMACS magnetic cell separator ...
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bioRxiv - Bioengineering 2022Quote: Mature mouse and human retinal organoids were dissociated using the embryoid body dissociation kit (Miltenyi Biotec, 130-096-348) with the gentleMACS™ Dissociator according to the manufacturer’s automated dissociation protocol with a minor modification ...
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bioRxiv - Neuroscience 2023Quote: ... The human iPSCs were grown on vitronectin and treated with small molecules LDN 193189 and A83-01 (Miltenyi Biotec) to induce a neural cell fate as well as with cyclopamine (Stem cell ...
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bioRxiv - Genomics 2024Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (Miltenyi Biotec; Cat. No. 130-050-201) and LS Columns (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... Human CD34+ cells were enriched as previously described on a CliniMACS Prodigy according to the manufacturer’s instructions (Miltenyi Biotec). Nonhuman primate CD34+ cells were harvested ...
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bioRxiv - Cancer Biology 2022Quote: Tumors and lungs harvested from mice were processed using the human tumor dissociation kit (Miltenyi Biotec, 130-095-929) with a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... a prototype multiplex bead-based EV Neuro platform (MACSPlex EV Kit Neuro, human, provided by Miltenyi Biotec, Bergisch Gladbach) was tested with different sample material and EV isolation protocols (Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were filtered to remove dead cells and B-cell depletion was performed using human CD19 microbead (Miltenyi Biotec) labeling according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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bioRxiv - Cancer Biology 2023Quote: ... CD138+ cells were isolated from bone marrow aspirate samples using Human CD138 MicroBeads (Miltenyi Biotec, Cat # 130-051-301). Selected cells were either viably cryopreserved in 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Developmental Biology 2023Quote: ... MNCs were resuspended in ice cold CliniMACS buffer supplemented with 0.5% human albumin or AutoMACS running buffer then labelled with anti-CD34 microbeads according to manufacturer’s instructions (Miltenyi Biotec). CD34+ cells were enriched using either a CliniMACS Plus (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Bioengineering 2024Quote: All samples were blocked for non-specific binding with human FcR Blocking Reagent (Miltenyi Biotec, cat.: 130-059-901) for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec). Digestion was stopped with 20% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... PBMC were incubated at 37ºC for 15 minutes with 0.5 μg/mL anti-human CD40 blocking antibody (Miltenyi Biotec) per well ...
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bioRxiv - Cancer Biology 2024Quote: ... and was dissociated into a single cell suspension using the Human Tumor Dissociation kit (Miltenyi Biotec, #130-095-929) on a gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... PE anti-human IgA stained bacteria were incubated with Anti-PE Magnetic Activated Cell Sorting (MACS) beads (Miltenyi Biotec) (1:5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Separation of mouse cells from human tumor cells was achieved using an LS column on a MACS Separator (Miltenyi Biotec). The flow-through fraction ...
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bioRxiv - Developmental Biology 2021Quote: ... the tip epithelial cells were isolated by EPCAM+ magnetic-activated cell sorting (MACS) beads according to the manufacturer’s instruction (CD326 MicroBeads, human, Miltenyi Biotec) from the distal lung tissues ...
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bioRxiv - Genetics 2020Quote: ... were washed in PBS and MACS buffer (PBS, 2 µM EDTA, 0.5% BSA) and stained with Human CD34 Microbead kit (Miltenyi Biotec) following the manufacturer’s instruction for 30 minutes (4 °C ...
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bioRxiv - Immunology 2021Quote: ... the biopsies were cut into 2-3mm pieces and dissociated using a human Tumour Dissociation Kit (Miltenyi Biotec, Surrey, UK) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Human CD34+ cells were then enriched in the sample by immunomagnetic beads using an AutoMACSpro (Miltenyi Biotec, Bergisch Gladbach, Germany). After collection ...
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bioRxiv - Immunology 2022Quote: RM CD4+ T cells were isolated from PBMCs using the non-human primate CD4+ T cells isolation kit (Miltenyi Biotec), activated using the NHP T-Cell Activation/Expansion kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated untouched using the magnetic human Pan-T-Cell Isolation Kit according to the manufacturer’s protocol (MACS, Miltenyi Biotec).
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bioRxiv - Microbiology 2019Quote: ... 2 to 3×108 PBMCs were taken up in 2.1 ml staining buffer (PBS, 0.5 % BSA, 2 mM EDTA) and 300 μl of anti-human CD61 and CD3 microbeads (Miltenyi Biotec) were added to remove contaminating platelets and T cells ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor tissues were mechanically and enzymatically dissociated using a human tumor dissociation kit (Miltenyi Biotec, Cat. No. 130-095-929), following the manufacturers recommendations ...
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bioRxiv - Immunology 2019Quote: CAR-and ΔLNGFR-positive T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... The second PBMC portion was labelled with magnetic microbeads coated with a cross-reactive anti-human CD14 mAb to isolate monocytes per the manufacturer’s instructions (Miltenyi Biotec) (14) ...