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Citations for Miltenyi Biotec :
451 - 500 of 652 citations for Recombinant Human ERN1 Protein GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... CD138+ cells were isolated from bone marrow aspirate samples using Human CD138 MicroBeads (Miltenyi Biotec, Cat # 130-051-301). Selected cells were either viably cryopreserved in 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Developmental Biology 2023Quote: ... MNCs were resuspended in ice cold CliniMACS buffer supplemented with 0.5% human albumin or AutoMACS running buffer then labelled with anti-CD34 microbeads according to manufacturer’s instructions (Miltenyi Biotec). CD34+ cells were enriched using either a CliniMACS Plus (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Bioengineering 2024Quote: All samples were blocked for non-specific binding with human FcR Blocking Reagent (Miltenyi Biotec, cat.: 130-059-901) for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec). Digestion was stopped with 20% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... The human iPSCs were grown on vitronectin and treated with small molecules LDN 193189 and A83-01 (Miltenyi Biotec) to induce a neural cell fate as well as with cyclopamine (Stem cell ...
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bioRxiv - Genomics 2024Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (Miltenyi Biotec; Cat. No. 130-050-201) and LS Columns (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2019Quote: ... immuno-purification was performed with µMACS GFP Tag Protein Isolation kits (Miltenyi Biotec). 1 mg of solubilized microsomes were loaded on µcolumns ...
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bioRxiv - Plant Biology 2022Quote: ... μMACS™ GFP or μMACS™ DYKDDDDK (FLAG) Protein Isolation Kits (Miltenyi Biotec) were used for immunoprecipitations ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 10 µM rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec) for the first 24 h after passaging ...
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bioRxiv - Cancer Biology 2021Quote: ... Separation of mouse cells from human tumor cells was achieved using an LS column on a MACS Separator (Miltenyi Biotec). The flow-through fraction ...
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bioRxiv - Developmental Biology 2021Quote: ... the tip epithelial cells were isolated by EPCAM+ magnetic-activated cell sorting (MACS) beads according to the manufacturer’s instruction (CD326 MicroBeads, human, Miltenyi Biotec) from the distal lung tissues ...
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bioRxiv - Genetics 2020Quote: ... were washed in PBS and MACS buffer (PBS, 2 µM EDTA, 0.5% BSA) and stained with Human CD34 Microbead kit (Miltenyi Biotec) following the manufacturer’s instruction for 30 minutes (4 °C ...
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bioRxiv - Immunology 2021Quote: ... the biopsies were cut into 2-3mm pieces and dissociated using a human Tumour Dissociation Kit (Miltenyi Biotec, Surrey, UK) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Human CD34+ cells were then enriched in the sample by immunomagnetic beads using an AutoMACSpro (Miltenyi Biotec, Bergisch Gladbach, Germany). After collection ...
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bioRxiv - Immunology 2022Quote: RM CD4+ T cells were isolated from PBMCs using the non-human primate CD4+ T cells isolation kit (Miltenyi Biotec), activated using the NHP T-Cell Activation/Expansion kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated untouched using the magnetic human Pan-T-Cell Isolation Kit according to the manufacturer’s protocol (MACS, Miltenyi Biotec).
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bioRxiv - Microbiology 2019Quote: ... 2 to 3×108 PBMCs were taken up in 2.1 ml staining buffer (PBS, 0.5 % BSA, 2 mM EDTA) and 300 μl of anti-human CD61 and CD3 microbeads (Miltenyi Biotec) were added to remove contaminating platelets and T cells ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumor tissues were mechanically and enzymatically dissociated using a human tumor dissociation kit (Miltenyi Biotec, Cat. No. 130-095-929), following the manufacturers recommendations ...
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bioRxiv - Immunology 2019Quote: CAR-and ΔLNGFR-positive T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... The second PBMC portion was labelled with magnetic microbeads coated with a cross-reactive anti-human CD14 mAb to isolate monocytes per the manufacturer’s instructions (Miltenyi Biotec) (14) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Multiple myeloma cells were then isolated from WBCs using human CD138 affinity magnetic beads (Miltenyi Biotec; cat#130-093-062) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from frozen PBMCs by using anti-CD19 immunomagnetic beads according to the manufacturer’s protocol (CD19 Microbeads, human, Miltenyi Biotec). Purified B cells were eluted in PBS x1 containing 0.5% v/v Fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: Freshly collected human decidual tissues were trimmed into 1 mm3 piece by GentelMACS Dissociator (Miltenyi Biotec, 130-093-235, Germany) and digested twice for 30 minutes each at 37°C with 1.0 mg/ml type IV collagenase (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then resuspended in wash buffer and incubated in MACS human FcR blocking solution (20μl/sample, cat nb. 130-059-901, Miltenyi Biotec) and primary antibodies against CD45-FITC (12μl/sample ...
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bioRxiv - Immunology 2021Quote: ... Human naïve B cells were isolated using the MACSxpress Whole Blood B Cell Isolation Kit (Miltenyi Biotec, 130-098-190) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used immediately for scRNA-seq ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in MACS buffer (PBS containing 2 mM ETDA and 2.5% FBS) supplemented with human FcR Blocking Reagent (Miltenyi Biotec) at 1:5 dilution and anti-human CD45-APC (BioLegend 304012 ...
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bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
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bioRxiv - Genomics 2022Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used for scRNA-seq ...
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bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were resuspended in binding buffer after centrifuged and sorted via human CD3 MicroBeads (Miltenyi Biotec, #130-050-101) according to the manufacturer’s recommendation (Note ...
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bioRxiv - Immunology 2022Quote: Treg and non-Treg cells were enriched using MACS CD4+CD25+CD127dim/− Regulatory T cell isolation Kit human (Miltenyi Biotec) from PBMCs of healthy donors and plated (1 × 105 cells per well ...
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bioRxiv - Genomics 2023Quote: Human induced pluripotent stem cell lines were routinely cultured on Matrigel-coated dishes in MACS iPS-Brew media (Miltenyi Biotec) and passaged using ReLESR (Stem Cell Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Neuroscience 2023Quote: ... Monocytes were then isolated using 250 μL of MicroBeads-conjugated human CD14 antibodies (StraightFrom LRSC CD14 MicroBead Kit; Miltenyi Biotec) added to 40 mL of cell suspension ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2023Quote: Human primary monocytes were isolated from healthy donors using the autoMACS Pan Monocyte Isolation Kit (Miltenyi Biotec, 130-096-537). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... All samples were blocked for non-specific binding with FcR Blocking Reagent for human (Miltenyi Biotec, cat.: 130-059-901) and mouse (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in MACS buffer (PBS, 0.5% BSA, 2 mM EDTA) for CD34+ magnetic selection using CD34 human MicroBeads (Miltenyi Biotec; cat ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from PBMC using Human CD4+ T Cell Isolation Kits (Miltenyi Biotec, Inc., Bergisch Gladbach, Germany). Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 30 min and stained with 2μl of human EpCAM (FITC conjugated, Miltenyi Biotec, 130-080-301) and 1:75 of mouse MHC class I (APC conjugated ...
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bioRxiv - Genetics 2024Quote: ... The PBMCs were further processed to isolate human primary T cells using MACS based PAN-T isolation kits (Miltenyi Biotec). RPMI-1640 (Gibco ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were immunoprecipitated by 50 µl Anti-HA MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) after incubation for 2.5 h at 4 °C while slowly rotating ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with 5 μL μMACS Protein G MicroBeads (Miltenyi Biotec), and incubated with shaking for 30 minutes at 4 ⁰C ...
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bioRxiv - Immunology 2022Quote: ... or S-protein (B.1.351) mutation peptide pool (Miltenyi Biotec, # 130-127-958, Germany) at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore ...
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bioRxiv - Immunology 2020Quote: ... Human CD4+ T-cells were isolated from peripheral blood lymphocytes using naïve CD4+ T-cell isolation kit from Miltenyi Biotec (Germany). The blood samples were obtained from Rheumatology Clinic at Saint Louis University with informed consent ...
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bioRxiv - Cell Biology 2019Quote: ... for no longer than 1 h before their enzymatical and mechanical dissociation with the Whole Skin Dissociation kit for human material (Miltenyi Biotec) and the Gentle MACS dissociator (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: Mouse and human brain samples were collected and mechanically dissociated using gentleMACS dissociation following the manufacturer’s instructions (Miltenyi Biotec, Paris, France). Freshly isolated tumor tissues were suspended in 5ml DMEM immediately after being surgically removed and placed in a petri dish ...