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Citations for Miltenyi Biotec :
451 - 500 of 718 citations for Interleukin 10 Receptor Beta IL10RB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with PE/Cy7 anti-human CD44 antibody (Miltenyi Biotec, 130-113-904, 1:50) and CD338-PE (ABCG2-PE ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads; Miltenyi Biotec). Next ...
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bioRxiv - Immunology 2021Quote: ... and subsequently incubated with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads; Miltenyi Biotec). Thereafter ...
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bioRxiv - Cancer Biology 2023Quote: ... To detect CD4, an antibody already labelled with FITC (130-113-775, clone REA623) from Miltenyi biotec was used at a concentration of 1:100 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained with mouse anti-human CD19 antibody (Miltenyi Biotec, cat# 130-113-730; 1:50) in the dark for 25 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies were diluted with Running Buffer in a MACSwell™ Deepwell Plates (Miltenyi Biotec, 130-126-865) in a total volume of 1050 µl ...
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bioRxiv - Plant Biology 2024Quote: ... 4% BSA) and incubated in a primary antibody solution anti-GFP-HRP (1/5000, MACS Miltenyi Biotec) and 1/5000 anti-His (11667475001 ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against CD14 (#130-110-583) and HLA-DR (#130-111-943) were obtained from Miltenyi Biotec. Dapi (#422801 ...
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bioRxiv - Cell Biology 2020Quote: ... and stimulated for 18 h with IL-2 (20 ng/ml; National Cancer Institute Preclinical Repository) and/or IL-12 (10 ng/ml; Miltenyi Biotec) cytokines ...
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bioRxiv - Cancer Biology 2022Quote: ... each blood sample was diluted in a 1 to 1 ratio with the HBSS buffer solution and incubated with 10% EpCAM MicroBeads (Miltenyi Biotec) with shaking at room temperature for an hour ...
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bioRxiv - Immunology 2021Quote: ... T cell activation by peptide was assessed according to the expression of eGFP regulated by the NFAT promoter and analysed on a MACSQuant Analyzer 10 (Miltenyi Biotec). The acquired data was processed by FlowJo (version 10.6.2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... All sample volumes were adjusted to 100 µl with 1x PBS + 10 µM ZnCl2 and measured via flow cytometry (MACSquant VYB, Miltenyi Biotec). The flow cytometer was calibrated using MacsQuant calibration beads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... were obtained by 7 days stimulation with 20 ng ml−1 macrophage colony-stimulating factor (M-CSF) and 10 ng ml−1 granulocyte-macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec).
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bioRxiv - Microbiology 2022Quote: ... BSA in PBS + 0.2 mM EDTA (FACS buffer) and subsequently resuspended in 100 μl FACS buffer containing 10 % (v/v) FcR blocking reagent (Miltenyi Biotec) and 1 % (v/v ...
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bioRxiv - Immunology 2020Quote: ... the corresponding mAbs were added for 30 minute and cells were washed with BD Perm/Wash Buffer 1X before acquisition in the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). The percentage of positive cells for CD107a ...
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bioRxiv - Immunology 2020Quote: ... Sorted cell subsets were co-cultured at a ratio 1:1 in 200µL RPMI/10% FBS and stimulated with CytoStim™ (Miltenyi Biotec). After 7 days’ culture ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were then washed twice prior to resuspension in FACS buffer and acquisition on the MACSQuant Analyzer 10 (Miltenyi Biotec). Data were analyzed with FlowJo software (v10 ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was washed with DMEM/10% FBS followed by CD11b positive selection (Miltenyi Biotec, Cat. No. 130–093-636) using mini-MACS (Miltenyi Biotec ...
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bioRxiv - Genomics 2020Quote: ... The cell suspension is pipetted through Falcon tubes with cell-strainer caps and then analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec) for 10,000 events.
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from 10 to 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec). Isolated cells were washed three times with PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal macrophages were isolated from adult (8-10 weeks old) female mice using Peritoneum Macrophage Isolation Kit (130-110-434, Miltenyi Biotec). Isolated cells were cultured in complete RPMI+GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The pelleted MVs were incubated with 10 μl of Biotin-conjugated anti-CD105 (Miltenyi Biotec cat# 130-094-916, RRID: AB_10828348) followed by adding 10 μl of anti-Biotin microbeads (MiltenyiBiotec cat# 130-094-256 ...
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bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
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bioRxiv - Immunology 2022Quote: ... in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). UCB or BM CD34+ HSPC subsets were directly seeded by FACS in the corresponding plates and cultured for 10 (bulk cultures ...
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bioRxiv - Immunology 2022Quote: ... in 96-well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). CD34+ HSPCs isolated from the BM of xenografted mice sacrificed between weeks 1-5 after grafting were directly seeded by FACS in the 96-well plates and cultured for 7 or 14 days before quantification of lineage outputs ...
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bioRxiv - Neuroscience 2023Quote: Analyses of the cells labeled in the previous section were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). Post-sort purity was assessed by the percent of CD11b+CD45+ singlets using MACSQuantify v2.13.0 software ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed twice and resuspended in 1mL of sorting buffer (up to 10⁸ cells) prior to magnetic separation on LS columns and the MACS Separator (Miltenyi Biotec). Columns were rinsed with 0.5mL of MACS buffer prior to loading the cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then centrifuged for 10 min at 3000 x g in the presence of debris removal solution (Miltenyi Biotec) and 10 min at 1000 x g in cooled PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... the suspension was counted and incubated with Anti-O4 MicroBeads at a concentration of 10 uL of beads per 1×107 cells (Miltenyi Biotec). After incubation ...
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bioRxiv - Immunology 2023Quote: ... 5 % CO2 after which migrated cells in the bottom chamber were collected and acquired on a MACSQuant Analyzer 10 Flow Cytometer using the MACSQuantify software (Miltenyi Biotec). The percentage of specific migration was calculated by dividing the number of cells migrated to the lower well by the total cell input (50 µl cell suspension directly measured on the MACSQuant Flow Cytometer).
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bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with Live/Dead dye and analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, San Diego, CA).
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and labeled with 10 μL streptavidin MACS microbeads (Miltenyi Biotec, 130-048-102) (sufficient for 1e7 cells) ...
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bioRxiv - Plant Biology 2020Quote: ... Eluted proteins were analysed by western blot using HRP-conjugated anti-myc antibody (130-092-113; Miltenyi Biotec), or an anti-HA antibody (3F10 ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were incubated overnight with an anti-GFP-HRP antibody (130-091-833, dilution 1:1000, Miltenyi Biotec). For FLAG detection ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with PE-conjugated CD133 antibody (1:50 dilution; Miltenyi Biotec cat. no. 130-113-108) and PerCP-Cy5.5-conjugated CD271 antibody (1:50 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were incubated with anti-cytochrome c-APC antibody (Clone#REA702, 1:50) (Cat#130111180, Miltenyi Biotec). Samples were analyzed on an LSR-II flow cytometer (BD Biosciences).
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bioRxiv - Pathology 2019Quote: ... MDSC and nonMDSC fraction were sorted using biotin-conjugated anti-Gr-1 antibody and streptavidin microbeads (Miltenyi Biotec) based on manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... resuspended in PBS and stained with anti-CD133 antibodies conjugated with APC fluorophore (Miltenyi Biotec 293C3, 1:50) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with anti-F4/80 antibodies conjugated with FITC/PE (Miltenyi Biotec 130-102-988, 130-102-943), and incubated in EasySep FITC/PE Positive Selection kit which labels FITC/PE epitopes with magnetic beads (Stemcell Technologies 18557 ...
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bioRxiv - Cancer Biology 2021Quote: Anti-CD133 (catalog no. 130-090-853) and PE conjugated anti-CD133 antibodies were purchased from Miltenyi Biotec. YTHDF2 (catalog no ...
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bioRxiv - Immunology 2022Quote: ... Isolated monocytes were analyzed by FACS using an anti CD14+ antibody (catalog 130-113-147, Miltenyi Biotec, Germany) to check their purity ...
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bioRxiv - Microbiology 2022Quote: ... Next PBMCs were then activated using 1 µg/mL immobilized CD3 and CD28 antibodies (Miltenyi Biotec, Cambridge, MA). After 3 days ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated with CD11b antibody conjugated magnetic beads (Miltenyi Biotec, Catalog #: 130-093-634 San Diego, CA) for 15 min and then passed through a magnetic bead column (Quadro MACS Separator and LS columns ...
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bioRxiv - Immunology 2020Quote: Mouse monoclonal antibodies against human CD86 (clone FM95) and DC-SIGN (clone DCN47.5) were purchased from Miltenyi Biotec. Antibodies against human CD83 (clone HB15e ...
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bioRxiv - Molecular Biology 2020Quote: ... patient cells were stained with the following primary antibodies: mouse anti–human CD3 (clone BW264/56; Miltenyi Biotec), mouse anti–human CD7 (clone M-T701 ...