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Citations for Miltenyi Biotec :
451 - 500 of 889 citations for Heat Shock Factor Protein 1 HSF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... with the latter two additionally analyzed by flow cytometry with suitable isotype antibodies (all: Miltenyi Biotec, Bergisch Gladbach, Germany) and unstained controls ...
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bioRxiv - Cancer Biology 2023Quote: ... To detect CD8, an antibody already labelled with PE-Vio-770 (130-113-721, clone REA734) from Miltenyi biotec was used at a concentration of 1:100 ...
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bioRxiv - Plant Biology 2023Quote: ... Western blotting was conducted as described above and visualized with anti GFP-HRP antibody (Miltenyi Biotec #130-091-833).
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bioRxiv - Molecular Biology 2023Quote: ... while antibody rounds were either optically bleached or signal was removed using the REAlease® Release Reagent (Miltenyi Biotec). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Primary Anti-EpCAM APC conjugated antibody with isotype control was used for all experiments (Miltenyi Biotec, Surrey, United Kingdom). Cells were blocked in 3% (w/v ...
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bioRxiv - Immunology 2023Quote: ... Collected PBMCs are resuspended with MACS buffer and appropriate antibodies conjugated with magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequent magnetic cell separation proceeded with a column in the magnetic field followed by a collection of unlabeled cells ...
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bioRxiv - Genetics 2023Quote: ... and enriched for iPSC-RPE via magnetically activated cell sorting using anti-CD24 and anti-CD56 antibodies (Miltenyi Biotec). iPSC-RPE cells in the negative cell fraction were seeded on the desired surface pre-coated with Laminin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Spleen qPCR samples were prepared by depletion of CD19+ TCRβ+ cells using biotinylated antibodies and Streptavidin MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... blood and peritoneal cavity cells were stained using the following antibodies: CD3 PerCP-Vio770 (130-119-656, Miltenyi Biotec), CD4-APC (130-123-207 ...
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bioRxiv - Cell Biology 2020Quote: ... was placed in MACS magnetic separator and the column was rinsed thrice with 1 mL rinsing solution (MACS BSA Stock Solution diluted 1:20 with autoMACS Rinsing Solution, Miltenyi Biotec). Beads bound to EVs were applied onto a magnetic column and Thy1-negative EVs that passed through the column were collected.
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bioRxiv - Cancer Biology 2022Quote: ... each blood sample was diluted in a 1 to 1 ratio with the HBSS buffer solution and incubated with 10% EpCAM MicroBeads (Miltenyi Biotec) with shaking at room temperature for an hour ...
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bioRxiv - Immunology 2020Quote: ... Sorted cell subsets were co-cultured at a ratio 1:1 in 200µL RPMI/10% FBS and stimulated with CytoStim™ (Miltenyi Biotec). After 7 days’ culture ...
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bioRxiv - Immunology 2022Quote: ... Cultures were activated with anti-CD2/CD3/CD28 coated beads at a bead to cell of ratio of 1:1 (Miltenyi Biotec). Cocultures were stained for viability with the LIVE/DEAD Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Neuroscience 2022Quote: ... serum-free conditions in 400 µl DMEM-F12 GlutaMax supplemented with Gibco™ Antibiotic-Antimycotic (1:100; Thermo Fischer Scientific) and NeuroBrews (1:50; Miltenyi Biotec). Small samples of cells were fixed and air-dried on glass slides to control for cell purity by immunocytochemical staining of GLUL ...
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bioRxiv - Neuroscience 2022Quote: ... serum-free conditions in DMEM-F12 GlutaMax supplemented with Gibco™ Antibiotic-Antimycotic (1:100; Thermo Fischer Scientific) and NeuroBrews (1:50; Miltenyi Biotec). After 24h or 48h ...
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bioRxiv - Microbiology 2019Quote: ... CD14+ cells were isolated by MACS using biotinylated anti-CD14+ antibody and anti-biotin microbeads according to the manufacture’s protocol (Miltenyi Biotec). Monocytes were cultured in complete RPMI plus 20 ng/ml recombinant human M-CSF for 7 days to promote differentiation into hMDMs ...
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bioRxiv - Developmental Biology 2022Quote: ... then bipotent progenitors were positively selected using a biotinylated EpCAM antibody (clone G8.8, eBiosciences) and streptavidin-conjugated magnetic beads (Miltenyi Biotec). This generates a >95% pure preparation of e16.5 bipotent alveolar progenitors (Sftpc+ Rage+ cells) ...
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bioRxiv - Genomics 2020Quote: ... and CD138+ multiple myeloma cells isolated from bone marrow biopsies by positive selection on antibody-conjugated magnetic beads (Miltenyi Biotec), were obtained with I.R.B ...
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bioRxiv - Neuroscience 2021Quote: ... kit with papain and double cell isolation using magnetic coupled antibodies anti-CD11B (Microglia) according to the manufacturer’s protocol (Miltenyi Biotec) and as previously described27 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The purity of PMNs was assessed by flow cytometry using APC anti-CD16 and FITC anti-CD15 antibodies (Miltenyi Biotec); markers characteristic of human neutrophils ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... then resuspended in PBE containing FITC-conjugated antibody specific for cardiac troponin T (Miltenyi Biotec, cat no. 130-119-575) at a concentration of 1:50 and incubated for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Dead cells were removed using magnetic bead-bound antibodies against apoptotic and necrotic cells (Dead cell removal kit, Miltenyi Biotec) and cells were washed twice ...
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bioRxiv - Genetics 2020Quote: ... BM freshly isolated mononuclear cells (MNC) were first stained with Lineage Cell Detection Cocktail-Biotinylated mouse antibody (MACS Miltenyi Biotec). For FACS analysis ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... cell selection for C-kit positive cells was performed with a CD117 antibody (Cat # 120-099-672, Miltenyi Biotec, Germany) in a Miltenyi Mini Macs System ...
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bioRxiv - Immunology 2021Quote: ... and Ter119 APC- or PE-labeled antibodies followed by magnetic depletion with anti-APC or -PE Micro-Beads (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes were depleted using Igκ/CD4/CD8/GR1/Ter119/TCRb/TCRgd/CD11b/CD11c/NK1.1 antibodies and anti-PE Micro-Beads (Miltenyi Biotec), and WT (CD45.1+CD45.2− ...
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bioRxiv - Immunology 2022Quote: ... The purity of PMNs was assessed by flow cytometry using APC anti-CD16 and FITC anti-CD15 antibodies (Miltenyi Biotec), markers characteristic of human neutrophils.
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bioRxiv - Plant Biology 2022Quote: ... Solubilized proteins from microsomal fraction were immunoprecipitated using super-paramagnetic micro MACS beads coupled to monoclonal anti-FLAG antibody according to the manufactureŕs instructions (Miltenyi Biotec). WT Col-0 extract was used as a control of the unspecific binding of endogen PIN2 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were resuspended in 180 μl artificial seawater containing 0.05% BSA and we added 20 μl of CD4 antibody (Miltenyi Biotec) conjugated with magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... and NK1.1(PK136) PE-conjugated antibody for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec) as described above ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T were purified using magnetic beads coated with anti-CD4 antibody (clone L3T4) according to the manufacturer’s instructions (Miltenyi Biotec). 5×105 2D2 CD4+ T cells were injected i.v ...
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bioRxiv - Immunology 2023Quote: ... and incubated with a biotinylated Ter-119 antibody (Table S1) and remaining erythrocytes were depleted with anti-biotin microbeads according to the manufacturer’s instructions (Miltenyi Biotec). Afterward ...
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bioRxiv - Immunology 2023Quote: Splenic CD4+ T cells derived from OT-II TCR-Tg mice were enriched with Biotin-labeled antibody cocktail (B220, CD8a, CD11b, CD11c, CD19, CD25, NK1.1, and TER-119) with Streptavidin Microbeads (Miltenyi Biotec). CD4+ T cells were further incubated with CD62L Microbeads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... Monocytes were then isolated using 250 μL of MicroBeads-conjugated human CD14 antibodies (StraightFrom LRSC CD14 MicroBead Kit; Miltenyi Biotec) added to 40 mL of cell suspension ...
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bioRxiv - Neuroscience 2023Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [29 ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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bioRxiv - Physiology 2024Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [14 ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Immunology 2019Quote: ... an anti-XCR1 (PE, REA707 1/200 Miltenyi Biotec) was used to confirm the identity of cDC1 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1:10 CD61-PE-Vio770 (Miltenyi Biotec, 130102627) for 1h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... CD11b (Clone M1/70, PE, 1:50, Miltenyi Biotec), Ly6G (clone 1A8 ...
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bioRxiv - Systems Biology 2020Quote: ... 1:10 Emb-PE (Miltenyi Biotec, 130-107-948). The staining was done in the dark at 4°C with agitation ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% v/v BSA (Miltenyi Biotec, 130-091-376), 0.5% v/v Triton-X (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ng/ml TGFβ (130-095-067, Miltenyi Biotec), 12.5 ng/ml IFN-γ (130-096-872 ...
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bioRxiv - Genomics 2020Quote: ... 1:100 B27 supplement without retinoic acid (Miltenyi Biotec) supplemented with 10 µM SB-431542 ...