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Citations for Miltenyi Biotec :
451 - 500 of 607 citations for F actin uncapping protein LRRC16A CARMIL1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... PBMC were incubated at 37ºC for 15 minutes with 0.5 μg/mL anti-human CD40 blocking antibody (Miltenyi Biotec) per well ...
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bioRxiv - Bioengineering 2024Quote: ... 25 µl of each sample was incubated with APC-labelled CD63 antibody (Miltenyi Biotec, Germany; 1 nM per well) overnight under dark conditions ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T cells were by counter-staining with anti-CD4-APC antibody (1:100) using flow cytometry (Miltenyi Biotec), and anti-CD3-FITC labelled antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... then bipotent progenitors were positively selected using a biotinylated EpCAM antibody (clone G8.8, eBiosciences) and streptavidin-conjugated magnetic beads (Miltenyi Biotec). This generates a >95% pure preparation of e16.5 bipotent alveolar progenitors (Sftpc+ Rage+ cells) ...
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bioRxiv - Genomics 2020Quote: ... and CD138+ multiple myeloma cells isolated from bone marrow biopsies by positive selection on antibody-conjugated magnetic beads (Miltenyi Biotec), were obtained with I.R.B ...
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bioRxiv - Neuroscience 2021Quote: ... kit with papain and double cell isolation using magnetic coupled antibodies anti-CD11B (Microglia) according to the manufacturer’s protocol (Miltenyi Biotec) and as previously described27 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
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bioRxiv - Genetics 2020Quote: ... BM freshly isolated mononuclear cells (MNC) were first stained with Lineage Cell Detection Cocktail-Biotinylated mouse antibody (MACS Miltenyi Biotec). For FACS analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... The stroma was negatively selected against epithelium using a EPCAM antibody coated microbeads (1:10; Miltenyi Biotec #130-061-101) and a Miltenyi MS column.
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... cell selection for C-kit positive cells was performed with a CD117 antibody (Cat # 120-099-672, Miltenyi Biotec, Germany) in a Miltenyi Mini Macs System ...
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bioRxiv - Immunology 2021Quote: ... and Ter119 APC- or PE-labeled antibodies followed by magnetic depletion with anti-APC or -PE Micro-Beads (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes were depleted using Igκ/CD4/CD8/GR1/Ter119/TCRb/TCRgd/CD11b/CD11c/NK1.1 antibodies and anti-PE Micro-Beads (Miltenyi Biotec), and WT (CD45.1+CD45.2− ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were resuspended in 180 μl artificial seawater containing 0.05% BSA and we added 20 μl of CD4 antibody (Miltenyi Biotec) conjugated with magnetic beads ...
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bioRxiv - Neuroscience 2023Quote: ... Monocytes were then isolated using 250 μL of MicroBeads-conjugated human CD14 antibodies (StraightFrom LRSC CD14 MicroBead Kit; Miltenyi Biotec) added to 40 mL of cell suspension ...
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bioRxiv - Immunology 2024Quote: ... Freshly isolated CD8+ T cells were then activated either with anti-CD3 and anti-CD28 antibody-coated microbeads at a 1:1 bead:cell ratio (Miltenyi Biotec) and with 100 U/ml IL-2 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [29 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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bioRxiv - Physiology 2024Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [14 ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Plant Biology 2022Quote: ... Solubilized proteins from microsomal fraction were immunoprecipitated using super-paramagnetic micro MACS beads coupled to monoclonal anti-FLAG antibody according to the manufactureŕs instructions (Miltenyi Biotec). WT Col-0 extract was used as a control of the unspecific binding of endogen PIN2 ...
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bioRxiv - Immunology 2023Quote: ... and NK1.1(PK136) PE-conjugated antibody for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec) as described above ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T were purified using magnetic beads coated with anti-CD4 antibody (clone L3T4) according to the manufacturer’s instructions (Miltenyi Biotec). 5×105 2D2 CD4+ T cells were injected i.v ...
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bioRxiv - Immunology 2023Quote: ... and incubated with a biotinylated Ter-119 antibody (Table S1) and remaining erythrocytes were depleted with anti-biotin microbeads according to the manufacturer’s instructions (Miltenyi Biotec). Afterward ...
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bioRxiv - Immunology 2023Quote: Splenic CD4+ T cells derived from OT-II TCR-Tg mice were enriched with Biotin-labeled antibody cocktail (B220, CD8a, CD11b, CD11c, CD19, CD25, NK1.1, and TER-119) with Streptavidin Microbeads (Miltenyi Biotec). CD4+ T cells were further incubated with CD62L Microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: Macrophages were isolated from lung single-cell suspensions using microbeads conjugated to anti-F4/80 antibodies according to the manufacturer’s protocol (Miltenyi Biotec). CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... These cells were then subjected to staining with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then stained with fluorochrome-conjugated antibodies (anti-mouse-BV421-CD45) and anti-mouse-PE-O4 (Miltenyi Biotec)) for 40 min at 4°C to characterize oligodendrocytes (CD45-O4+) ...
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bioRxiv - Plant Biology 2024Quote: ... Immunoprecipitation was carried out using micromagnetic bead-coupled GFP antibodies from µMACS GFP Isolation Kit (Miltenyi Biotec 130-091-125) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Cells underwent a TCRαβ depletion step (anti-TCRαβ antibody REA652 clone, anti-biotin microbeads and LD column, all Miltenyi Biotec) with an overnight recovery followed by cryopreservation in 45% TexMACS media (Miltenyi Biotec) ...
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bioRxiv - Physiology 2024Quote: ... Cells were filtered through 35 μm filters and incubated with CD31 magnetic conjugated bead antibodies (#130-097-418, Miltenyi Biotec). Positive selection for CD31+ cells was performed using magnetic separator columns (#130-042-201 ...
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bioRxiv - Molecular Biology 2024Quote: ... and stained with antibodies against the following murine and human surface markers: murine CD45 (1/50 mCD45-VioBlue; Miltenyi Biotec), human CD45 (1/50 hCD45-APCvio770 ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were further incubated overnight at 4°C with anti-PD1 antibody (Miltenyi Biotec, 130-117-384; 1:100) and anti-CK antibody (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... from the spleen of naive mice after enrichment of CD11c+ cells using a PE-conjugated anti CD11c antibody and magnetic-activated cell sorting (Anti-PE MicroBeads, Miltenyi Biotec). For in vitro functional analysis ...
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
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bioRxiv - Microbiology 2020Quote: ... BMDCs were enriched by depletion of BMMs using biotinylated α-F4/80 antibody (eBioscience, RRID: AB_466657) and anti-biotin conjugated magnetic beads (Miltenyi Biotec). The BMDCs in the flow through were positively selected using α-CD11c magnetic beads according to the manufacturer’s protocol (Mil405 tenyi Biotec).
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bioRxiv - Cancer Biology 2021Quote: Murine liver primary ECs were isolated from athymic nude mice also using MACS microbead-conjugated antibodies for murine CD31 (Miltenyi Biotec) and cultured in MV2 EC culture medium as described above ...
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bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and incubated in 2% FBS in PBS with anti-ROR1-APC antibody (1:25, #130-119-860, Miltenyi Biotec) on ice for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The second (positive) magnetic separation was then performed using astrocyte-specific anti-GLAST (ACSA-1) antibodies conjugated to the magnetic beads (Miltenyi Biotec). Anti-O4 selection using magnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: ... MSCs in passage two were detached by accutase treatment and stained with a human MSC phenotyping kit and an anti-HLA-DR antibody (both Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... thymocytes were isolated from adult mice by negative magnetic selection using biotinylated anti-CD4 and anti-CD8 antibodies and streptavidin (SAV) microbeads (Miltenyi Biotec). To isolate fetal progenitor cells ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... slides were incubated for 1 h at room temperature in the dark with a cardiac troponin-T antibody conjugated to APC (130-120-543; Miltenyi Biotec) prepared at a 1:100 dilution in PBS ...
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bioRxiv - Plant Biology 2022Quote: ... The blots were then incubated at room temperature with the monoclonal α-GFP antibody (1:5000) (Miltenyi Biotec, 130-091-833), α-RFP antibody (1:5000 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells were enriched from the filtrates by Ficoll density gradient centrifugation and magnetic-activated cell sorting using CD34 antibody-conjugated microbeads (Miltenyi Biotec). CD34+ cells were grown in serum-free StemSpan SFEMII (Stemcell Technologies ...
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bioRxiv - Systems Biology 2021Quote: ... were stimulated for 3 days in 96 well plate with MACSi-Beads loaded with anti-mouse CD3 and anti-mouse CD28 antibodies (AP beads, Miltenyi Biotec), at a 1:1 proportion ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM EDTA) and stained with 1 μl of an APC-conjugated CD21 specific antibody (clone: HB5; 130-101-739, Miltenyi Biotec). The cells were incubated at 4 °C in the dark for 20 min and washed with and resuspended in 200 μl FACS staining buffer ...