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Citations for Miltenyi Biotec :
451 - 491 of 491 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Tissue samples were homogenized 2 × 55 sec in 1 ml sterile phosphate buffer saline (PBS) using a gentleMACS™ Octo Dissociator (Miltenyi Biotec). Total DNA from bacterial cells and infected tissues was purified using a GeneJet Genomic DNA Purification Kit (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... A total of 1.5 × 106 PBMCs were stimulated with 1 µg/mL of peptide pools for 6 h in TexMACS™ GMP Medium (Miltenyi Biotec), supplemented with 5% AB human serum (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... The anti-IgA-stained bacteria were then incubated in 1 mL of staining buffer containing 50 μL of anti-PE magnetic activated cell sorting (MACS®) beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Molecular Biology 2024Quote: ... pooled honey bees (n=30) were dissociated with 15 mL of molecular grade water using gentleMACS™ M Tubes (Miltenyi Biotec, USA) and a gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: ... For genomic and transcriptomic analyses of activated LCs fresh migrated LCs from 3 donors were stimulated with TNF-α (25 ng/ml, Miltenyi Biotec, UK) for 2 ...
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bioRxiv - Immunology 2022Quote: ... The pellet was resuspended in 0.5 mL DPBS with 0.5 % BSA and labeled with 50 μL anti-CD14 microbeads (Miltenyi Biotec, catalog# 130-050-201). Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: ... Differentiated BMDMs were re-plated on glass coverslips within 24-well tissue culture plates in complete medium and polarized or not with murine recombinant IL-4 (20 ng/ml, Miltenyi Biotec, CA, USA) for 48 h ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
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bioRxiv - Microbiology 2023Quote: Approximately half of the lung and the left hemisphere of each brain were weighed and homogenized in 1 mL of DMEM supplemented with 2% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein complexes were then eluted into 1.5 mL Eppendorf tubes using 3 x 35 µL of pre-heated 95°C elution buffer (Miltenyi Biotec #130-042-701). The samples were heated to 95°C for 5 min ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions of mouse lungs were generated by putting lungs in gentle MACS™ C tubes and mechanically homogenizing in 5 ml PBS using a gentle MACS™ Octo Dissociator (Miltenyi Biotec) in the absence of enzymes ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were adjusted to 5 mg/mL protein concentration and incubated for 1 h at 4 °C with 100 μL of anti-GFP magnetic beads (Miltenyi Biotec, #130-091-125). Bound proteins were analyzed by immunoblotting with anti-GFP (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was removed and the SVF resuspended in 10 ml room temperature plain DMEM followed by additional filtering through a 30 μm mesh filter (Miltenyi Biotec # 130-041-407) and subsequent centrifugation at 700 g for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Alveolar-like MDMs were generated by differentiating CD4+ monocytes on tissue culture plates into macrophages in the presence of 50 ng/ml of human M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days ...
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bioRxiv - Developmental Biology 2019Quote: ... NeutrAvidin-functionalized devices were incubated with either (1) 2 μg/ml biotinylated mouse anti-human anti-SSEA-5 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany) (2 ...
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bioRxiv - Bioengineering 2019Quote: ... a concentration of 107 retinal cells per 90 μl of DBPS/BSA buffer (Dulbecco’s PBS containing 0.5 mg ml−1 BSA) was incubated with 10 μl of the RGC-specific marker CD90.1 microbeads (Miltenyi Biotec, GmbH, and Bergisch Gladbach, Germany) at room temperature for 30 min and then washed with 5 ml of DPBS/BSA buffer ...
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bioRxiv - Immunology 2019Quote: ... centrifuged at 500g for 10 min at 4 °C and resuspended in 0XTD supplemented with 100 ng/ml each of Stem Cell Factor (SCF, Miltenyi Biotec, 130-096-695), Flt3 Ligand (Flt3L ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Differentiation was induced when cells reached 80-90% confluency using BPEL medium (Ng et al, 2008) supplemented with 20 ng/mL Activin-A (Miltenyi Biotec, #130-115-012), 20 ng/mL BMP4 (R&D systems ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in fresh media containing either 100 nM CCK or 100 nM saline vehicle control up to 72 hours with concurrent treatment with a mouse cytokine cocktail containing 50-ng/mL TNFα (Miltenyi Biotec, #139-101-687), 10-ng/mL IL-1β (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Open-repertoire T cells (7.5e106 cells per well of a 24 well plate) were stimulated with OKT3 (50 ng mL−1, Miltenyi Biotec, Cat 130-093-387) and rhIL-2 (300 IU mL−1 ...
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bioRxiv - Immunology 2022Quote: ... The pellet was resuspended in 50 ml EDTA-supplemented (2mM) washing medium and transferred over a 100 µm SmartStrainer (Miltenyi Biotec, Bergisch Gladbach, Germany). The cell suspension was then diluted to 90 ml ...
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bioRxiv - Immunology 2022Quote: We stimulated the spleen and lung cells from immunized and control animals to detect nucleocapsid protein-specific CD8+ T cell responses with an N protein-peptide pool at a concentration of 1.5 μg/mL/peptide (Miltenyi Biotec; 130-126-699, Germany) at 37 °C for 16-18 h followed by the addition of Brefeldin A (5 μg/ml BD Biosciences #BD 555029 ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were differentiated into a macrophage-like phenotype (MDM, monocyte derived macrophages) using M-CSF (50 ng/mL, Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days at 37ºC and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Canada) for 48 hours or T-cell TransAct (10 uL for each mL of culture, Cat # 130-111-160, Miltenyi Biotec, Bergisch Gladbach, Germany) for 72 hours respectively.
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bioRxiv - Bioengineering 2024Quote: ... frozen liver samples were added to ice-cold PBS at 50 mg/mL and processed for tissue homogenization using the gentleMACS Dissociator (n = 3 per group) (Miltenyi Biotec, Bergisch Gladbach, Germany). Then ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Neuroscience 2022Quote: ... At the end of the incubation the single cell suspension was washed with 500 μL wash buffer (0.02% BSA in 1X PBS with 80 U/mL Protector RNase Inhibitor) and passed through a 20 μM filter (MACS, Miltenyi Biotec cat#130-101-812) to a fresh 2 mL Protein LoBind Eppendorf ...
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bioRxiv - Physiology 2019Quote: ... A 100 mg of depilated skin sample was homogenized thoroughly in 2.0 ml of acetonitrile in a gentleMACS™ M tube using a gentleMACS™ Dissociator (Miltenyi Biotec Inc., CA, USA). Each sample was subjected to three cycles of the preprogrammed homogenization protocol Protein_01.01 (a 55 s homogenization cycle with varying speeds and directions of rotation) ...
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bioRxiv - Developmental Biology 2022Quote: ... and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]) and passed through a 40 μm filter (Greiner Bio-One ...
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bioRxiv - Bioengineering 2023Quote: ... Media supplements tested were FGF-2 at a concentration of 10 ng/ml (Human FGF-2 IS premium grade, MACS Miltenyi Biotec, Germany, 130-104-922), EGF at a concentration of 10 ng/ml (Recombinant Human EGF ...
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bioRxiv - Immunology 2023Quote: ... Each lung was then fragmented using surgical scissors and transferred to 5 mL of dissociation media (1 lung per dissociation reaction) in gentleMACS™ C Tubes (Miltenyi Biotec, 130-093-237). Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... liver and brain samples were homogenised in PBS (1-3 mL, adjusted to tissue weight) using gentleMACS™ M tubes (Miltenyi Biotec Ltd, Bisley, Surrey) and gentleMACS™ octo dissociator using the standard protein program ...
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bioRxiv - Immunology 2023Quote: ... target U937 cells (killing-sensitive) and control K562 cells (killing-resistant) were pre-treated with soluble anti-CD3 monoclonal antibody (mAb; OKT3, Miltenyi Biotec, at 200 ng/ml) for 15 min at 37°C prior to co-culture with primary human CD8+ T cells ...
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bioRxiv - Microbiology 2023Quote: MEFs were seeded at 5.104 cells per well in 24-well plates one day before stimulation and treated with 300 IU/mL IFNα (Miltenyi Biotec Cat#130-093-131) and incubated for up to 24 hours.
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bioRxiv - Genetics 2022Quote: ... 150 µl of 1M HEPES and 0.75 µl of 10 mg/ml DNAse I) and dissociated in gentleMACS Dissociator (Miltenyi Biotec Inc., cat. no. 130-093-235) using the Multi_E_01.01 program ...