Labshake search
Citations for Miltenyi Biotec :
451 - 500 of 872 citations for 5 Azepane 1 sulfonyl 2 methoxy benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... Freshly isolated CD8+ T cells were then activated either with anti-CD3 and anti-CD28 antibody-coated microbeads at a 1:1 bead:cell ratio (Miltenyi Biotec) and with 100 U/ml IL-2 (BioLegend ...
-
bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... samples were cut into approximately 2 – 4mm chunks with forceps and a scalpel and transferred to a gentleMACS C tube (Miltenyi Biotec, 130-093-237) containing 4.7 ml RPMI 1640 base media ...
-
bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
-
bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
-
bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
-
bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
-
bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
-
bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
-
bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
-
bioRxiv - Neuroscience 2024Quote: ... The tissue was then minced and transferred into a 2 ml Dounce homogenizer containing nuclei extraction buffer (130-128-024, Miltenyi Biotec, Bergisch Gladbach, Germany) along with 0.2 U/ml Ribolock RNase inhibitor ...
-
bioRxiv - Genomics 2024Quote: ... THP-1 in vitro differentiated macrophages were then stimulated by replacing media with 2 mL of fresh media containing 100 ng/mL of IFNγ (Miltenyi Biotec, 130-096-484) for 4 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... or CSF-1 (Miltenyi Biotec Australia Pte Ltd), were added at 100ng/ml ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1:20 with autoMACS Rinsing Solution (Miltenyi Biotec). The cells were fixed adding 400 µl of Paraformaldehyde 4% ...
-
bioRxiv - Microbiology 2020Quote: ... Pumorphamine 1 µM (Miltenyi Biotec, 130-104-465), Ascorbic Acid 200ng/µL ...
-
bioRxiv - Cell Biology 2020Quote: ... CHIR99021 (0.9 μM or 1 μM, Miltenyi Biotec), SB431542 (10 μM ...
-
bioRxiv - Cancer Biology 2022Quote: ... and Vectofusin-1 (#130-111-163, Miltenyi Biotec). Briefly ...
-
bioRxiv - Cell Biology 2022Quote: ... Pdgfra (Miltenyi Biotec #130-101-905, 1:100), Ki67 (Abcam #16667 ...
-
bioRxiv - Bioengineering 2024Quote: ... and 1 ng/ml IL-15 (Miltenyi Biotec) for 48 hrs ...
-
bioRxiv - Bioengineering 2024Quote: ... and 1% MACS supplement (Miltenyi Biotec, Gaithersburg, MD). Cells were cultured until day 4 ...
-
bioRxiv - Microbiology 2023Quote: ... and 1:50 FcR Blocking Reagent (Miltenyi Biotec)) ...
-
Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... CD31-VioBright 515 (1:200; Miltenyi Biotec, REA784), CD45-FITC (1:200 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 20 ng ml−1 SCF (Miltenyi Biotec, 130-093- 991 ...
-
bioRxiv - Neuroscience 2023Quote: ... intracellular staining for vGLUT1 (1/200, Miltenyi Biotec, #130-120-764 ...
-
bioRxiv - Molecular Biology 2024Quote: ... human CD11b (1/100 CD11b-APC; Miltenyi Biotec), human CD19 (1/100 CD19-BV510 ...
-
bioRxiv - Molecular Biology 2024Quote: ... human CD45 (1/50 hCD45-APCvio770; Miltenyi Biotec). BM cells were also stained with human CD3 (1/50 CD3-APC ...
-
bioRxiv - Molecular Biology 2024Quote: ... human CD15 (1/50 CD15-PE; Miltenyi Biotec), human CD11b (1/100 CD11b-APC ...
-
bioRxiv - Microbiology 2024Quote: ... and 1:200 FcR blocking reagent (Miltenyi Biotec) in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... was placed in MACS magnetic separator and the column was rinsed thrice with 1 mL rinsing solution (MACS BSA Stock Solution diluted 1:20 with autoMACS Rinsing Solution, Miltenyi Biotec). Beads bound to EVs were applied onto a magnetic column and Thy1-negative EVs that passed through the column were collected.
-
bioRxiv - Cancer Biology 2022Quote: ... each blood sample was diluted in a 1 to 1 ratio with the HBSS buffer solution and incubated with 10% EpCAM MicroBeads (Miltenyi Biotec) with shaking at room temperature for an hour ...
-
bioRxiv - Microbiology 2021Quote: ... were obtained by 7 days stimulation with 20 ng ml−1 macrophage colony-stimulating factor (M-CSF) and 10 ng ml−1 granulocyte-macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec).
-
bioRxiv - Immunology 2020Quote: ... Sorted cell subsets were co-cultured at a ratio 1:1 in 200µL RPMI/10% FBS and stimulated with CytoStim™ (Miltenyi Biotec). After 7 days’ culture ...
-
bioRxiv - Immunology 2020Quote: ... Cells were stimulated by the addition of individual virus-specific peptide pools (1 µg/ml) for 6 h in the presence of a blocking CD40 antibody (1 µg/ml; Miltenyi Biotec). For subsequent MACS-based enrichment of CD154+ ...
-
bioRxiv - Immunology 2022Quote: ... Cultures were activated with anti-CD2/CD3/CD28 coated beads at a bead to cell of ratio of 1:1 (Miltenyi Biotec). Cocultures were stained for viability with the LIVE/DEAD Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2023Quote: ... at a final concentration of 1 µg/mL in the presence of a blocking CD40 antibody (1 µg/mL; Miltenyi Biotec) and CD28 antibody (1 µg/mL ...
-
bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue, REAfinity [Miltenyi Biotec, Germany]). Samples were analyzed using a S1000 Flow Cytometer with an A600 96-well plate high-throughput extension (Stratedigm ...
-
bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
-
bioRxiv - Immunology 2021Quote: ... and the respective SARS-CoV-2 peptide pool (0.6 nmol/peptide, S and N pools from Miltenyi Biotec, 130-126-701 and 130-126-699). Unstimulated samples were used for subtraction of background cytokine production ...
-
bioRxiv - Developmental Biology 2022Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
-
bioRxiv - Developmental Biology 2023Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the beads were removed and IL-2 was replaced with IL-7 and IL-15 (Miltenyi Biotec GmbH, 130-095-367 and 130-096-567) at 10 ng/ml each ...
-
bioRxiv - Neuroscience 2022Quote: ... serum-free conditions in 400 µl DMEM-F12 GlutaMax supplemented with Gibco™ Antibiotic-Antimycotic (1:100; Thermo Fischer Scientific) and NeuroBrews (1:50; Miltenyi Biotec). Small samples of cells were fixed and air-dried on glass slides to control for cell purity by immunocytochemical staining of GLUL ...
-
bioRxiv - Neuroscience 2022Quote: ... serum-free conditions in DMEM-F12 GlutaMax supplemented with Gibco™ Antibiotic-Antimycotic (1:100; Thermo Fischer Scientific) and NeuroBrews (1:50; Miltenyi Biotec). After 24h or 48h ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 1:10 CD61-PE-Vio770 (Miltenyi Biotec, 130102627) for 1h at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... CD11b (Clone M1/70, PE, 1:50, Miltenyi Biotec), Ly6G (clone 1A8 ...
-
bioRxiv - Systems Biology 2020Quote: ... 1:10 Emb-PE (Miltenyi Biotec, 130-107-948). The staining was done in the dark at 4°C with agitation ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1% v/v BSA (Miltenyi Biotec, 130-091-376), 0.5% v/v Triton-X (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 ng/ml TGFβ (130-095-067, Miltenyi Biotec), 12.5 ng/ml IFN-γ (130-096-872 ...