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Citations for Miltenyi Biotec :
451 - 500 of 541 citations for 2 2 2'' 6'' Dichloro 4'' hydroxphenylamino phenyl d4 N N dimethylacetamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in clusters and passaged every 4-5 days using StemMACS Dissociation reagent (Miltenyi Biotec). All iPS lines were genotyped using an RFLP at the relevant important loci and the sequence was confirmed by Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... cut into 4 pieces and enzymatically digested by using the whole skin dissociation kit from Miltenyi biotec without enzyme P for 5 hours at 37°C under agitation (500 rpm) ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 50 ng/ml GM-CSF and 20 ng/ml IL-4 (Miltenyi Biotec). Untouched peripheral blood cDC1 and cDC2 (cDCs ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec). Human cells were then cytospinned with Shandon cytofunnels (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with HBSS and spun at 300 g for 5min at 4 °C (Miltenyi Biotec). The pellet was resuspended and incubated with 80 µL of MACS buffer and 20 µL of anti-IgM microbeads (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2024Quote: ... 4% BSA) and incubated in a primary antibody solution anti-GFP-HRP (1/5000, MACS Miltenyi Biotec) and 1/5000 anti-His (11667475001 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec) and isolated by positive magnetic selection according to manufacturer’s instructions (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... and stored at 4°C before being acquired using the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec) and analyzed using FlowJo 10 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated with anti-APC magnetic beads (Miltenyi Biotec, Catalog No. 130-090-855, 1/4) for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... tumor portions were minced to 3-4 mm pieces and dissociated using a Tumor Dissociation Kit (Miltenyi Biotec), followed by the clearance of red blood cells with RBC lysis buffer (eBioscience ...
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bioRxiv - Genetics 2020Quote: ... following the manufacturer’s instruction for 30 minutes (4 °C) before being passed successively through two LS Columns (Miltenyi Biotec) with three MACs buffer washes ...
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bioRxiv - Microbiology 2020Quote: ... and the medium on the monocytes was replenished with X-vivo 15 + L-glutamine containing interleukin-4 (Miltenyi Biotec) and granulocyte-macrophage colony-stimulating factor (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... sliced into 4 sagittal sections and placed into gentleMACS C-tubes (#130-093-237, Miltenyi Biotec, San Diego, CA), and processed for generation of single-cell suspensions using the Adult Brain Dissociation Kit and GentleMACS Octo Dissociator system (#130-107-677 and #130-095-937 ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Physiology 2023Quote: ... at 4°C for 15 min under continuous agitation and passed through MS column (130-042-201, Miltenyi Biotec). The CD146 positive cells ...
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bioRxiv - Cancer Biology 2022Quote: ... and 37°C incubator and passaged every 4-5 days in small clusters using StemMACS Dissociation reagent (Miltenyi Biotec). Colon organoids were differentiated as previously described[46] with minor modifications ...
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bioRxiv - Genomics 2024Quote: ... 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot no. 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...
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bioRxiv - Immunology 2023Quote: ... then incubated for 15 min at 4 °C in labeling buffer containing streptavidin-conjugated and anti-FITC MicroBeads (Miltenyi Biotec). Cells were negatively selected using MACS LD columns per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Immunology 2024Quote: ... IMhu-M cells were cultivated in IL-4/GM-CSF MoDC differentiation medium (Miltenyi Biotec, Cat. No. 130-094-812), when indicated ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... and 4% BSA in HBSS) was added per tissue and tissues were further homogenized with the GentleMACS dissociator (Miltenyi Biotec) using the following program ...
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bioRxiv - Immunology 2023Quote: ... and NK1.1(PK136) PE-conjugated antibody for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec) as described above ...
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bioRxiv - Immunology 2024Quote: ... conjugate and staining information are as follows: anti-human CD3 (Miltenyi Biotec, Clone BW264/56, APC-R700, 1:200, 4℃); anti-human CD19 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were further incubated overnight at 4°C with anti-PD1 antibody (Miltenyi Biotec, 130-117-384; 1:100) and anti-CK antibody (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 minutes at 4°C with rotation and passed through an LS separation column (Miltenyi Biotec, 130-042-401) inserted into a Quadro MACS Multi Stand magnetic stand (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stored at 4°C in dark until the next morning for data acquisition using a MacQuant10 (Miltenyi Biotec, USA). Data was analyzed using FlowJo 10 (BD Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Genetics 2021Quote: ... and 20 μL of each sample was incubated for 30 min at 4°C with rat anti-mouse CD45-VioBlue (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... The adherent monocytes were then washed twice with PBS and resuspended in RPMI supplemented with the cytokines GM-CSF (1000 U/mL) and IL-4 (500 U/mL) (Miltenyi Biotec). Differentiation to immature MDDCs was achieved by incubation at 37 °C with 5% CO2 for 7 days and subsequent activation with cytokines addition every other day ...
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bioRxiv - Immunology 2020Quote: ... Cells then were fixed with 4% paraformaldehyde (final concentration) in phosphate-buffered saline for 20 min and analyzed on the MACSQuant 10 (Miltenyi Biotec) or the LSRFortessa X20 (Becton Dickinson Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
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bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting cell suspension was spun down for 5 min at 300 × g and 4°C and processed for MACS using the CD11b/c microbeads according to the manufacturers protocol (Miltenyi Biotec). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with the following antibodies for 30 minutes at 4°C: CD3 VioGreen (clone BW264/56, Miltenyi Biotec), CD14 V500 (clone M5E2 ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured with 100 ng/mL (500 UI/mL) GM-CSF plus 50 ng/mL (250 UI/mL) IL-4 (Miltenyi Biotec) at 106cells/mL in complete RPMI medium supplemented with 10% FCS (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were collected and centrifuged at 500 x g for 5 min at 4°C and CD8 T cells were isolated by magnetic sorting according to the manufacturer’s protocol (Miltenyi Biotec, Germany). To select transduced CD8 T cells ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was carried out for 30 min in the dark at room temperature with anti-4-1BB-PE (Miltenyi Biotec), anti-CD40L- PEVio770 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... CD8 T cells from autologous blood and tonsils from 4 HLA-A*02:01 donors were enriched using negative selection magnetic beads (CD4, CD19 depletion, Miltenyi Biotec). Roughly 1.5 e6 enriched CD8s were stained with Fc block for 10 minutes on ice ...