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Citations for Viagen Biotech :
1 - 50 of 70 citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... T cells were lysed using DirectPCR (cell) (Viagen Biotech, LA, USA) lysis buffer supplemented with proteinase K (10 µg/mL ...
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bioRxiv - Immunology 2021Quote: ... 58α-β- T cell hybridomas were lysed using DirectPCR Tail Lysis Buffer (Viagen Biotech) with proteinase K (SIGMA ...
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bioRxiv - Immunology 2023Quote: ... 58α-β- T cell hybridomas were lysed using DirectPCR Tail Lysis Buffer (Viagen Biotech) with proteinase K (SIGMA ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from CD8+ T cells using the DirectPCR Lysis solution (Viagen Biotech) containing Proteinase K and target regions were amplified by PCR using the GoTaq G2 PCR mastermix (Promega) ...
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bioRxiv - Immunology 2019Quote: Sorted CD4+ and CD8+ T cells were lysed in DirectPCR Lysis Reagent (mouse tail) (Viagen Biotech) with 1mg/ml proteinase K (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μL DirectPCR (#102-T, Viagen) with 10 μL proteinase K (50 μg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was extracted after lysing cells with 200μl of DirectPCR lysis reagent (Viagen biotech, 102-T) for 15min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed with 500 μL cold PBS and then lysed with 100 μL DirectPCR Lysis Reagent (Viagen Biotech, 102-T) with 1% Proteinase K (Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were digested in Direct PCR mix (Viagen #102-T) with 1.5% Proteinase K (Quiagen #19133 ...
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bioRxiv - Developmental Biology 2021Quote: ... Genotypes were determined by polymerase chain reaction (PCR) after O/N digestion using DirectPCR (Tail) Lysis buffer (Viagen, Los Angeles, CA) per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... tail samples were lysed by using DirectPCR tail solution (Viagen 102 T) with overnight incubation at 55°C ...
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bioRxiv - Cell Biology 2023Quote: ... were lysed in Direct PCR (Tail) lysis buffer (Viagen, Biotech, #102-T) supplemented with 40 µg/mL Proteinase K (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was lysed by incubation in tail lysis buffer (Viagen, 102-T) with 0.1 mg/ml proteinase K (Diamond ...
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bioRxiv - Cell Biology 2023Quote: ... at 37°C using DirectPCR Lysis Reagent (mouse tail) (VIG102-T, Viagen Biotech). PCR for Pax7-nGFP was performed using primer Pax7nGFP.F/ Pax7nGFP.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Molecular Biology 2024Quote: ... After adding 200 μl of Direct PCR Tail (Lysis buffer) (Viagen, 102-T) and 4 μl of proteinase K (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Ear biopsies were lysed at 55℃ overnight in DirectPCR solution (Viagen, 102-T) followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix ...
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bioRxiv - Biophysics 2021Quote: ... U2OS cDNA from selected clones was isolated with DirectPCR Lysis Reagent (Viagen 101-T), treated with 0.5 mg/ml proteinase K for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... gDNA was extracted overnight at 55°C in Tail Lysis Buffer (Viagen 102-T) with Proteinase K (Roche 03115828001 ...
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bioRxiv - Physiology 2021Quote: ... Mouse ear biopsies were taken and digested in DirectPCR lysis reagent (Viagen Biotech, 102-T) with proteinase K (Qiagen ...
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bioRxiv - Physiology 2021Quote: Tail samples were digested in a 200:2 ratio of Tail lysate (VIAGEN 102-T) to proteinase K overnight in 55°C water bath overnight and then inactivated at 85°C for 45 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNA from clones was isolated using DirectPCR (Tail) reagent (Viagen, catalog no. 102-T) and Vcan exon 2 and exon 3 were amplified using Phusion Taq (NEB ...
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bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102-T), 1x Taq buffer (Syd Labs #MB042-EUT) ...
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bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102- T), 1× Taq buffer (Syd Labs #MB042-EUT) ...
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bioRxiv - Genetics 2022Quote: ... Clipped fin tissue was digested in 30 μl DirectPCR Lysis Reagent (Mouse Tail) (Viagen, 102-T) with 40 μg/ml Proteinase K (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... Genomic DNA was obtained by lysing ear punches in DirectPCR Ear Lysis Reagent (Viagen, 102-T) according to the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from ear punches was extracted by incubating samples with DirectPCR Lysis Reagent (Viagen 102-T) and Proteinase K at 55°C overnight ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA: single worm DNA extract with lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
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bioRxiv - Developmental Biology 2023Quote: ... tail or ear clips were taken and incubated with DirectPCR lysis buffer (Viagen Biotech; Cat#102-T) containing 10µg/mL proteinase K (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: Tail tips of mice were lysed in 200 μl of DirectPCR Lysis Reagent (Viagen Biotech, Cat# 102-T) with proteinase K (0.2 mg/ml ...
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bioRxiv - Cancer Biology 2024Quote: Tail snips collected from 2.5-week-old mice were lysed in DirectPCR Lysis Reagent (Viagen Biotech, 102-T) and Proteinase K (Millipore Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... All genotyping was performed in house using ear punch tissue lysed overnight in DirectPCR Lysis Reagent (Viagen, 102-T) and Proteinase K (Sigma ...
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bioRxiv - Immunology 2023Quote: Mice were tail clipped upon weaning and the tail samples were dissolved in DirectPCR Lysis Reagent (Viagen Biotech; #102-T), as outlined in the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Individual nematode was isolated and washed with 10% bleach for 10 seconds and transferred into with 200µl PCR tube containing lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
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bioRxiv - Cell Biology 2020Quote: ... Genotyping was carried out on genomic DNA derived from adult and embryonic tails using the DirectPCR Lysis Reagent (102-T, Viagen Biotech) and EconoTaq Plus Green 2X Master Mix (Lucigen) ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic tail snips or lung tissue was incubated in 0.2mg/mL Proteinase K in DirectPCR Lysis Reagent (Viagen Biotech 101-T) at 55°C for 4 hr ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse genotype was determined by PCR analysis of DNA extracted from tail snip using a lysis reagent (Viagen Biotech, Cat 102-T) and proteinase K digestion (55°C ...
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bioRxiv - Physiology 2024Quote: ... tissue samples were collected via tail clipping and DNA was extracted using Direct PCR Lysis Reagent (Cat no. 101 101 T, Viagen Biotech, USA), and 1.5 µL of the lysate from each sample was used for PCR ...
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bioRxiv - Physiology 2024Quote: ... Genotyping was performed via PCR from tail clip biopsies after proteinase K digestion in DirectPCR tail lysis buffer (Viagen Biotech 102-T). PCR of genomic DNA was performed using Apex Taq Master Mix and allele-specific primers as previously described73.
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bioRxiv - Cell Biology 2020Quote: ... The mice genotype was determined by PCR analysis of DNA extracted from tail snip using a lysis reagent (Viagen Biotech, Cat 102-T) and proteinase K digestion (55°C ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA was isolated from toe clips by incubating in DirectPCR (Tail) Lysis Reagent for Genotyping Crude Lysates (cat# 102-T; Viagen Biotech, Los Angeles, CA, USA) with proteinase K (cat# DSP50220-0.1 ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA was isolated from a 5 mm tail biopsy using a rapid digestion method where 95 μl of lysis buffer reagent (Viagen Biotech, Los Angeles, CA; Cat: 102-T) and 5 μl of Proteinase K (20 mg/ml ...
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bioRxiv - Immunology 2022Quote: Lysis: Sorted cells were lysed in 40 µl DirectPCR Lysis Reagent (Cell) from Viagen Biotech with 0.4 mg Prot K ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspending the cells in a lysis solution (100 μL DirectPCR Lysis Reagent (Cell) (Viagen Biotech), 76 μL of water and 4 μL Proteinase K ...
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bioRxiv - Molecular Biology 2022Quote: ... resuspending the cells in a lysis solution (100 μL DirectPCR Lysis Reagent (Cell) (Viagen Biotech), 76 μL of water and 4 μL Proteinase K ...
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bioRxiv - Cancer Biology 2022Quote: ... a snap frozen tumoroid cell pellet was lysed using DirectPCR Lysis Reagent for Cells (Viagen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells pellets were lysed and gDNA extracted using DirectPCR lysis reagent CELL (Viagen, 301-C) and Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were generated using cell lysate for each cell line as template (DirectPCR lysis reagent, Viagen), and then purified by using QIAquick 96 PCR Purification Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... DNA from cell populations less than 200,000 cells was processed via direct lysis using DirectPCR lysis buffer (Viagen Biotech). 200ng DNA or the whole cell lysis from each cell population underwent 28-cycle PCR using Phusion High-Fidelity DNA Polymerase (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... as single cells into 96-well plates to establish clonal populations and subsequently genotyped for deletions using DirectPCR Cell Lysis buffer (Viagen), and cells positive for deletions were expanded (Fig ...
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bioRxiv - Genomics 2023Quote: ... cells were lysed in DirectPCR lysis reagent (Viagen). Lyastes were subsequently assessed by running qPCR with primers annealing to the transposon sequences ...