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Citations for Viagen Biotech :
1 - 50 of 53 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Purified DNA: single worm DNA extract with lysis buffer (8µl direct PCR lysis reagent (Viagen, #102-T), 1 µl 5mg/ml Proteinase K ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping was performed using genomic DNA prepared from ear punch biopsies with the Direct DNA (Tail) (Viagen Biotech, USA). The Kcnq2Thr274Met/+ animals were maintained and studied on the 129Sv genetic background ...
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bioRxiv - Developmental Biology 2021Quote: ... Genotypes were determined by polymerase chain reaction (PCR) after O/N digestion using DirectPCR (Tail) Lysis buffer (Viagen, Los Angeles, CA) per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA was isolated using Viagen Direct PCR (Viagen). The presence of oncogenic and silent mutations in exons and insertions or deletions in introns of BRAF ...
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bioRxiv - Immunology 2019Quote: Genomic DNA was extracted using DirectPCR lysis reagent (Viagen) with proteinase K (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Genomic DNA was isolated using DirectPCR lysis buffer (Viagen). Gene disruption was analysed by PCR using primers spanning the HIF1α 5’ UTR Sense 5’-cagtgctgcctcgtctga– 3’ and Antisense 5’ caatcccattaacgccgagg-3’ ...
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bioRxiv - Neuroscience 2019Quote: ... DNA extracted from toe biopsies (Lysis buffer and proteinase K, Viagen) was amplified using a Scn1a-specific forward primer (TMF 441-TTCCATCCCAAGGAAATACCATGT ...
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bioRxiv - Immunology 2021Quote: ... genomic DNA was extracted using the DirectPCR Lysis solution (Viagen Biotech) containing Proteinase K and target regions were amplified by PCR using the GoTaq G2 PCR mastermix (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA for PCR was extracted using DirectPCR Lysis Reagent (Viagen Biotech) plus 10 µg/ml proteinase K (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was isolated with direct PCR lysis reagent (Viagen Biotech) with Proteinase K (O/N 55 °C) ...
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bioRxiv - Genetics 2020Quote: ... Genomics DNA were prepared using the DirectPCR Lysis Reagent (Viagen Biotech Inc.). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA isolation was performed using DirectPCR Lysis Reagent (Cell) (Viagen Biotech), Proteinase K (EO0491 ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated using 20 μL of DirectPCR Lysis Reagent Cell (Viagen) supplemented with 1:100 10 mg/mL Proteinase K (Bioline) ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated using 20 μL of DirectPCR Lysis Reagent Cell (Viagen) and sgRNA target sites were amplified for TIDE analysis as described above (using primers SM8+9 (LAD2) ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA of was prepared using 1,000 μl Direct PCR lysis reagent (Viagen), 50 μl proteinase K solution (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was extracted either using DirectPCR Lysis Reagent (Viagen Biotech, 301-C) or Puregene Core Kit B (Qiagen) ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNA from clones was isolated using DirectPCR (Tail) reagent (Viagen, catalog no. 102-T) and Vcan exon 2 and exon 3 were amplified using Phusion Taq (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from CD8+ T cells using the DirectPCR Lysis solution (Viagen Biotech) containing Proteinase K and target regions were amplified by PCR using the GoTaq G2 PCR mastermix (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: Zebrafish were genotyped using DNA extracted from fin clipped tissue using DirectPCR lysis reagent (Viagen) complemented with 0.1 mg ml-1 proteinase K ...
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bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102-T), 1x Taq buffer (Syd Labs #MB042-EUT) ...
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bioRxiv - Physiology 2023Quote: ... PCR reaction mixture contained 1uL tail DNA in DirectPCR Lysis reagent (Viagen Biotech #102- T), 1× Taq buffer (Syd Labs #MB042-EUT) ...
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bioRxiv - Cancer Biology 2020Quote: ... ears of mice were clipped and genomic DNA was extracted using DirectPCR Lysis Reagent (Viagen Biotech). PCR amplification was performed using GoTaq Green Master Mix (Promega ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted using 30 μL of DirectPCR lysis reagent (Viagen Biotech, Los Angeles, CA) mixed with 30 μL of water containing 12 μg of proteinase K ...
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bioRxiv - Synthetic Biology 2022Quote: ... Genomic DNA was obtained by lysing ear punches in DirectPCR Ear Lysis Reagent (Viagen, 102-T) according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... PCR using genomic DNA extracted from embryonic sacs digested with DirectPCR Lysis Reagent (Viagen, 301-C) containing Proteinase K (final concentration 6 U/mL ...
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bioRxiv - Neuroscience 2023Quote: Genotyping was done as follows: Genomic DNA was extracted from tail biopsies using PCR reagent (Viagen) supplemented with Proteinase K (1 mg/mL) ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from ear punches was extracted by incubating samples with DirectPCR Lysis Reagent (Viagen 102-T) and Proteinase K at 55°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA of single-cell colonies was isolated using DirectPCR lysis solution (Viagen Biotech, Los Angeles, CA), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was extracted after lysing cells with 200μl of DirectPCR lysis reagent (Viagen biotech, 102-T) for 15min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from cells collected from 96-well plates using Direct PCR lysis reagent (Viagen) supplemented with 1 mg/mL (w/v ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was released from mouse tail biopsies by incubation in DirectPCR Lysis Reagent (Tail) (Viagen Biotech, Inc.) at 85°C ...
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bioRxiv - Immunology 2021Quote: ... Mice were routinely genotyped using genomic DNA extracted from tail biopsies using the Direct PCR Lysis tail reagent (Viagen) supplemented with 5 mg/ml proteinase K (Worthington) ...
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bioRxiv - Cell Biology 2022Quote: ... Mice were routinely genotyped using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5□mg/mL proteinase K (Worthington) ...
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bioRxiv - Immunology 2022Quote: ... DNA from cell populations less than 200,000 cells was processed via direct lysis using DirectPCR lysis buffer (Viagen Biotech). 200ng DNA or the whole cell lysis from each cell population underwent 28-cycle PCR using Phusion High-Fidelity DNA Polymerase (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: Whole embryos or fin clips from adult fish were genotyped by resuspending tissue in DirectPCR® DNA-Tail solution (Viagen), and heating samples to 56°C for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened by PCR using primers that flank the SUMO4 gene using genomic DNA purified with direct PCR buffer (Viagen). Clones that displayed reduced size of SUMO4 PCR product were sequenced by Sanger sequencing to confirm disruption of the SUMO4 locus ...
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bioRxiv - Cell Biology 2022Quote: Mice were genotyped by NGS using genomic DNA extracted from ear biopsies with the Direct PCR Lysis tail reagent (Viagen) and 5 mg/mL proteinase K (Worthington) ...
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bioRxiv - Cancer Biology 2023Quote: In-house genotyping was done using DNA extracted from tail or ear tissue using DirectPCR Lysis Reagent (Viagen Biotech, Inc). Thermal cycling was performed using the Power SYBR Green 2X Master Mix (ThermoFisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... Established colonies were manually picked and expanded for screening indel mutations using PCR amplification of a region spanning the targeted gRNA region (genomic DNA isolated using DirectPCR Lysis Reagent (Tail) (VIAGEN), primers ...
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bioRxiv - Cell Biology 2020Quote: ... Genotyping of LRP5 KO and AXIN2 KO mutants was done following genomic DNA isolation (DirectPCR Lysis Reagent; 301-C, Viagen Biotech) by PCR using DreamTaq DNA Polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Single cell clones were expanded and genomic DNA was extracted using Direct PCR Lysis reagent (Viagen, 201-Y; 25 µl/well) with Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... genomic DNA was isolated by enzymatic digestion of tail clips using Direct-PCR Lysis Reagent (Viagen Biotech, Los Angeles, CA, USA). Genotypes were determined by performing PCR amplification of genomic DNA using allele-specific primers ...
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bioRxiv - Cell Biology 2020Quote: ... Genotyping was carried out on genomic DNA derived from adult and embryonic tails using the DirectPCR Lysis Reagent (102-T, Viagen Biotech) and EconoTaq Plus Green 2X Master Mix (Lucigen) ...
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bioRxiv - Microbiology 2023Quote: ... Mouse genotypes were assessed from genomic DNA isolated from ear clips via digestion in DirectPCR Digestion Buffer + Proteinase K (Viagen Biotech) using the following primer pairs ...
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bioRxiv - Immunology 2023Quote: ... Mouse genotypes were assessed by PCR from genomic DNA isolated from ear clips via digestion in DirectPCR Digestion Buffer + Proteinase K (Viagen Biotech) using the following primer pairs ...
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bioRxiv - Physiology 2023Quote: Genomic DNA (obtained from a 2mm ear punch) was extracted using DirectPCR Lysis Reagent (Mouse tail) (Viagen Biotech, Los Angeles, CA) and PCR-grade proteinase K (Roche #RPROTK-RO ...
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bioRxiv - Cancer Biology 2024Quote: Mouse genotypes were determined by PCR analysis of DNA derived from earclips or embryonic tails using direct tail lysis buffer (Viagen Biotech) and Proteinase K ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse genotype was determined by PCR analysis of DNA extracted from tail snip using a lysis reagent (Viagen Biotech, Cat 102-T) and proteinase K digestion (55°C ...
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bioRxiv - Physiology 2024Quote: ... tissue samples were collected via tail clipping and DNA was extracted using Direct PCR Lysis Reagent (Cat no. 101 101 T, Viagen Biotech, USA), and 1.5 µL of the lysate from each sample was used for PCR ...
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bioRxiv - Neuroscience 2022Quote: ... Genotyping of Fgd4fl/+ mice was performed by PCR on DNA isolated from tail biopsies using Direct PCR lysis reagent (Viagen Biotech Inc., USA), following the manufacturer’s instructions ...