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Citations for Becton, Dickinson and Company :
301 - 350 of 1551 citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Slides were blocked and incubated with the following primary antibodies for 1 hour at RT in humidity chamber (anti-CldU, Abcam ab6326, 1:100; anti-IdU, BD 347580 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated for 1 h at RT with primary antibody used as follows: mouse anti-EEA1 (BD Biosciences: 610457) 1 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... at 3:100 or phospho-AKT (pS473) at 1:20 dilutions for 30 mins at RT before analysis on FACSAria II (BD).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1% TritonX100 in PBS for 20 min at RT and stained overnight with rat-anti-PECAM1/CD31 antibodies (1/100, clone Mec13.3, BD Pharmingen) in blocking solution and secondary Alexa594 goat anti-rat IgG antibodies and DAPI (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340, BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by blocking with 3 % normal goat serum (NGS) in PBS for 60 min at RT and incubation with primary antibodies (mouse anti-RAN, 1:500, BD-610340 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were first blocked for non-specific antigen binding (incubating for 20 min at RT with 2 µg/ml CD16/CD32 Rat anti-Mouse, unlabeled, Clone: 2.4G2, BD, 553142) then antibodies were added without washing off the blocking solution ...
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bioRxiv - Immunology 2020Quote: ... for 5 min at RT prior to staining with a surface stain cocktail containing the following antibodies purchased from BD and Biolegend ...
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bioRxiv - Evolutionary Biology 2022Quote: The cryosections were blocked with 1% skim milk in PBS with 0.5% Tween-20 at RT for 15 min and incubated with primary antibody (rat anti-mouse anti-PECAM-1 [CD31; BD Pharmingen ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards, they were washed twice by centrifuging (500 g, 5min, RT) and resuspending in Stain Buffer (#554656, BD Pharmingen, USA). Cells were fixed in Fixation Buffer (#554655 ...
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bioRxiv - Cell Biology 2023Quote: ... pH 6.5) and specifically stained for 15 min at RT using an antibody against GPVI (HY101-PE, #565241, BD PharmingenTM) at a ratio of 1:10 ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by 3 times DPBS wash and then analyzed through FACS Calibur I (BD Bioscience). For the determination of sialic acid ...
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bioRxiv - Bioengineering 2019Quote: ... washed 3 times with PBS and analyzed by flow cytometry (Accuri™C6, BD Biosciences). The median fluorescence intensity (MFI ...
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bioRxiv - Cell Biology 2022Quote: ... fixed BM cells were washed and permeabilized three times in Perm/wash buffer (BD Biosciences) supplemented with 0.1% TritonX100 and further incubated in the same buffer with primary antibodies overnight at 4C ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (BD OptEIA Substrate Solution, BD Biosciences) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (BD OptEIA Substrate Solution, BD Biosciences) for 5 min ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed 7 times and developed with substrate according to the manufacturers’ instructions (BD). The reaction was stopped with 2M sulfuric acid and absorbance read at 450 nm.
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bioRxiv - Cell Biology 2020Quote: ... washed two times with FACS buffer and then analyzed with an Accuri C6 instrument (BD). The data were analyzed using FlowJo ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed three times with PBS then blocked with 5% skimmed milk (BD Difco) in PBS for 1 Hr at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... PBMCs were collected at same time points using CPT tubes (BD-Biosciences, San Jose, CA) containing citrate ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested at the indicated time points and tested by ELISA for CXCL8 (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... and passed several times through a syringe with a 23 G needle (BD Microlance 3). Next ...
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bioRxiv - Microbiology 2022Quote: ... The pellets were washed 3 times with PBS and subjected to flow cytometry (BD Biosciences). Double-stained yeasts were mother cells ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed three times and analysed by Flow cytometer (BD LSRFortessa X-20). The data were analysed with FlowJo software.
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (BD OptEIA Substrate Solution, BD Biosciences) for 5 min ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed two times with buffer and applied on a FACS Fortessa (BD Biosciences). Routinely ...
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bioRxiv - Cell Biology 2023Quote: ... cells were harvested at indicated time-points and fixed with neat Cytofix/Cytoperm (BD Biosciences) for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell death detection in the sub-G1 peak were performed by flow cytometry as we previously described using FACSCalibur (BD Biosciences)[69] ...
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bioRxiv - Developmental Biology 2022Quote: ... Flow cytometric analysis was performed on a BD FACSAria II with special order 445nm laser for CFP detection when using Zebrabow (BD Biosciences). Gates were drawn using negative and isotype matching controls ...
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bioRxiv - Physiology 2021Quote: INS-1 cells were collected for flow cytometry analysis using FITC Annexin V apoptosis detection reagent (BD Biosciences, San Jose, CA). The apoptotic cells were measured by FACS Count™ Flow Cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated with detection biotinylated anti-IFNγ antibody for 2h and followed by incubation with streptavidin-horse radish peroxidase (BD Biosciences) for 1h ...
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bioRxiv - Immunology 2020Quote: ... The cytokine stain prepped cells were then intracellularly stained with a cytokine detection panel consisting of: α-IFNγ (XMG1.2, 612769, BD Biosciences), α-IL-10 (JES5-16E3 ...
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bioRxiv - Immunology 2020Quote: ... IFNγ-producing cells were stained with IFNγ Detection Antibody in conjunction with cell surface markers (listed below) and analyzed on an LSRFortessa flow cytometer (BD Biosciences). Antibodies used included CD3e (145-2C11) ...
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bioRxiv - Immunology 2021Quote: ... Parasites were then washed with FACS buffer and placed on ice for incubation with anti-isotype detection antibodies depending on application: anti-IgG3-BV421 (R40-82, BD Bioscience), anti-IgM-PE/Cy7 (RMM-1 ...
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bioRxiv - Immunology 2022Quote: Splenocytes from infected mice were incubated for 5 hours at 37°C 5%CO2 in cRPMI prior intra-cytoplasmic detection of active-caspase 3 (BD Bioscience) using BD Fixation/Permeabilization kit (BD Bioscience) ...
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bioRxiv - Molecular Biology 2022Quote: ... 24 hours post electroporation medium was changed in order to remove inhibitors and 7 days later KI frequencies were determined through GFP detection by flow cytometry using the BD LSR-Fortessa Cell Analyzer (BD Bioscience). At the same timepoint cell viability and live cell counts were determined using the Cellometer Auto 2000 (Nexcelom).
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bioRxiv - Biochemistry 2023Quote: ... the cells were resuspended with 500 μl PBS containing 1% BSA for detection in BD Accuri C6 flow cytometer system (BD Biosciences) at excitation 488 nm and emission 519 nm ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were then washed with FACS buffer and placed on ice for incubation with anti-isotype detection antibodies depending on application: anti-IgG3-BV421 (R40-82, BD Bioscience), anti-IgM-PE/Cy7 (RMM-1 ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured by a SPOT RT monochrome charge-coupled device camera (Diagnostic Instruments, Sterling Heights, MI) and processed by IPLab imaging software (BD Biosciences) and Photoshop CS3 (Adobe ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured by a SPOT RT monochrome charge-coupled device camera (Diagnostic Instruments, Sterling Heights, MI) and processed by IPLab imaging software (BD Biosciences) and Photoshop CS3 (Adobe ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with pS6 or HK2 antibody for 1 h and O-GlcNAc antibody for 20 min at RT in the dark in cytoplasmic permeabilization buffer (BD Biosciences). Nuclear permeabilization buffer was used for c-Myc staining ...
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bioRxiv - Immunology 2021Quote: ... Single cells were index sorted into 384 well plates containing 1uL of reverse transcription (RT) master mix using a FACS Aria III with FACS Diva software (BD Biosciences) to maintain protein expression with plate well location ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were stained in a solution containing 1 μg/ml PI and 4 μg/ml RNase A in PBS at 2 x 106 cells/ml for a minimum of 30 min at RT Cell cycle analysis was performed on a LSR Fortessa analyser (BD Biosciences) and analysed using FlowJo software.
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bioRxiv - Immunology 2019Quote: ... After washing in PBS and incubated for 1 hour at RT with secondary donkey-anti-rat rhodamine red 1:100 (both from BD Pharmingen) in antibody buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C and secondary antibodies for 30 min in RT in Perm/Wash buffer (554714, BD Biosciences) containing 5% FBS ...
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bioRxiv - Immunology 2020Quote: ... the following antibodies and viability dye were incubated with PBMCs at RT for 20 min: anti-CD3 BUV395 (clone SP34-2; BD Biosciences), anti-CD4 BUV495 (clone SK3 ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The sections were then blocked and permeabilized with 5% Donkey serum 0.01% Triton X-100 for 30 min at RT before being incubated with rabbit anti-YAP dilution 1/100 (Signalling Technology #14074) and mouse anti beta-catenin (BD 610153) (dilution 1/200 ...
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bioRxiv - Immunology 2021Quote: ... Single cells were index sorted into 384 well plates containing 1uL of reverse transcription (RT) master mix using a FACS Aria III with FACS Diva software (BD Biosciences) to maintain protein expression with plate well location ...
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bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized with BD Fix/Perm for 20 min at room temperature (RT) and stained intracellularly with antibodies in 1x Perm buffer (BD Biosciences): anti-IFN-γ (1:100 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated with an intracellular antibody panel for 30 min at RT in 1x permeability/wash buffer (BD #554723), including NeuN-APC (1:50) ...