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Citations for Becton, Dickinson and Company :
1 - 50 of 1557 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Stable integration was confirmed after sorting EGFP+ cells 3-4 days after transfection (BD FACS Aria). Protein was produced from IL-2 and IL-12 expressing stable lines during one-week culture in serum-free media (Freestyle 293 ...
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bioRxiv - Systems Biology 2021Quote: ... by subcloning the EGFP cassette from pDONR233-eGFP (itself a derivative of pEGFP-C1, BD Biosciences). All the constructs were validated by restriction digest and DNA sequencing of the subcloned fragments.
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bioRxiv - Bioengineering 2021Quote: ... Infected cells grew for 3 days and then infection efficiency was measured using flow cytometry to measure EGFP (BD Accuri C6); the dose that gave rise to 5 - 15% EGFP+ cells was selected for each LSR for further experiments ...
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bioRxiv - Microbiology 2024Quote: ... cells were sorted to select double fluorescence positive clones (eGFP-Lysenin and mOrange-Galectin-3) using a BD FACSAria III Cell Sorter (BD Biosciences). A polyclonal subpopulation with high expression of eGFP-Lysenin was further selected for expansion.
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bioRxiv - Developmental Biology 2023Quote: ... the relative percentage of eGFP+ FTW-ESCs-eGFP were determined using an LSR II Flow Cytometer (BD Bioscience). Total ES cell number (tN ...
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bioRxiv - Molecular Biology 2024Quote: ... BFP and EGFP fluorescence were analyzed by BD FACS Aria III or Beckman CytoFLEX S ...
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bioRxiv - Immunology 2024Quote: ... Downstream analyses were performed by gating on EGFP+ cells (for flow cytometry-based assays) or by sorting for EGFP+ cells using BD FACSymphony S6 Cell Sorter (BD Biosciences) (for Western blot experiments) ...
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bioRxiv - Developmental Biology 2023Quote: ... Anterior cells (mCherry-positive plus EGFP-negative) or posterior cells (EGFP-positive) were purified by FACS (FACSAria III Cell Sorter, BD Biosciences) using a 100 μm low pressure nozzle and collected into separate tubes of amphibian culture medium 50 ...
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bioRxiv - Immunology 2022Quote: ... cells were sorted on EGFP on a FACSAria (BD) to obtain a >95% transduced population.
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... EGFP-positive cells were enriched by FACS-sort (BD FacsAria III Sorter ...
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bioRxiv - Neuroscience 2023Quote: ... eGFP-expressing CD4-positive cells were detected by BD FACSCanto™ II flow cytometry (BD Biosciences).
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bioRxiv - Bioengineering 2024Quote: ... Nanoparticle delivery and uptake (fluorescent NPs) or eGFP expression (γPNA/DNA NPs in the 654-eGFP mouse) was measured using a BD FACSCalibur (BD Biosciences; Franklin Lakes, NJ). Flow cytometry data obtained was analyzed using FlowJo software (FlowJo ...
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bioRxiv - Molecular Biology 2021Quote: ... expression of EGFP and mCherry fluorescence were analyzed by BD FACS Aria III or BD LSRFortessa X-20 ...
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bioRxiv - Neuroscience 2024Quote: ... 3) mouse isotype IgG1 anti-C-terminal binding protein 2 (CtBP2, 1:100, BD Transduction Laboratories #612044), 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... the GFP-expressing bicistronic reporter construct pIRES2-EGFP (BD Biosciences Clontech) was used ...
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bioRxiv - Immunology 2020Quote: ... eGFP+ cells were isolated using a FACSAria IIu cell sorter (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... the EGFP positive cells were sorted by FACS (BD FACSAria SORP), and total RNA was extracted using MolPure® Cell RNA Kit (Yeasen ...
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bioRxiv - Microbiology 2023Quote: ... 3 loopfuls of biomass grown at 30°C on Brain-Heart Infusion (BHI) agar (BD Biosciences, NJ, USA) were collected and mixed with a phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... for 3 hours at 37°C in the presence of GolgiStop (monensin, according to manufacturer’s recommendation, BD Biosciences). Cells were washed and labelled with fixable viability dye (LIVE/DEAD eFlour780 ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP-positive cortical cells were selected using the FACSAria II (BD Biosciences). Cells were stained with DAPI to select against cell debris ...
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bioRxiv - Physiology 2021Quote: ... The PCR product was cloned into a pIRES2-EGFP expression vector (BD Biosciences Clontech Franklin Lakes ...
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bioRxiv - Molecular Biology 2021Quote: ... 50,000 of both EGFP and mCherry positive cells were sorted by BD FACS Aria III for RNA extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-positive cells were isolated using a FACSAria III sorter (BD Biosciences). Genomic mutations were confirmed by immunoblotting and sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence of EGFP and PI was measured by FACS Calibur (BD Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... mCherry and/or EGFP positive cells on a FACSAria II (BD Biosciences). FACS cell isolation was used for bulk and single cell RNA-seq ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 3 hours at 37°C with 5% CO2 in R10 buffer supplemented with 0.33 μl/ml GolgiStop (BD Biosciences) and 15 μg/ml DNase I ...
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bioRxiv - Synthetic Biology 2022Quote: ... the levels of eGFP values were determined by the flow cytometer (BD Biosciences) equipped with a high-throughput sampler ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were sorted for eGFP expression 48 h after transfection (FACSaria II, BD). Colonies were picked 5 days later ...
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bioRxiv - Genomics 2021Quote: ... infection was monitored by EGFP expression using flow cytometry (BD Accuri C6 plus). Replicates were independent reactivations from a single experiment.
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in cold phosphate-buffered saline (PBS) containing 3% FBS and stored at 4°C until analysis using a LSRII flow cytometer (BD Biosciences). FlowJo software v10 (Treestar ...
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bioRxiv - Immunology 2020Quote: ... followed by staining for 3 days at 37 °C with 1:100 dilutions of BV421-labeled mouse anti-human CD35 clone E11 (BD Biosciences) and Alexa Fluor 488-labeled mouse anti-Ki67 clone B56 (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and ionomycin (500 ng/mL) for 3 hrs at 37°C prior to surface and intracellular staining using the BD Cytofix/Cytoperm Kit (BD Biosciences). Intracellular transcription factor staining was performed using a Foxp3 staining kit (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: Splenocytes from infected mice were incubated for 5 hours at 37°C 5%CO2 in cRPMI prior intra-cytoplasmic detection of active-caspase 3 (BD Bioscience) using BD Fixation/Permeabilization kit (BD Bioscience) ...
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bioRxiv - Immunology 2024Quote: ... in 75μl of 1xPBS for one hour were seeded on coated coverslips for 3 min at 37°C and fixed using Cytofix/Cytoperm-Fixation/Permeabilization Kit (BD biosciences). Coverslips were then gently washed two times with 1x PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Spermatogonia were sorted based on Pou5f1:EGFP fluorescence using a FACSAria II (BD Biosciences) and collected in PBS.
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bioRxiv - Cancer Biology 2020Quote: Meiotic and kinetochore genes tagged with Enhanced Green Fluorescent Protein (EGFP) (from BD Biosciences) were used ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then analyzed for EGFP fluorescence on an LSRII flow cytometer (BD Biosciences), gated for viable ...
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bioRxiv - Immunology 2022Quote: ... BFP+ TMBP cells and EGFP+ TMBP cells were analysed using a FACS VERSE (BD) or LSRFortessa (BD ...
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bioRxiv - Neuroscience 2021Quote: ... EGFP-positive cells were isolated with a fluorescence activating cell sorter (FACSAria, BD Biosciences) by the following three parameters ...
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bioRxiv - Genetics 2022Quote: ... Samples were sorted for mCherry and eGFP fluorescence using a FACS Aria II (BD) or CytoFLEX SRT (Beckman Coulter ...
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bioRxiv - Immunology 2024Quote: ... Cells were sorted for high EGFP expression on a BD FACSAria III (BD Biosciences).
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bioRxiv - Bioengineering 2024Quote: ... The EGFP-loaded CMs were also measured by FACS (LSR Fortessa X20, BD Biosciences) for the EGFP intensity within the CMs ...
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bioRxiv - Synthetic Biology 2024Quote: ... The quantification of eGFP positive cells was performed by flow cytometry (BD LSRFortessaTM, BD).
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bioRxiv - Synthetic Biology 2024Quote: ... The quantification of eGFP positive cells was performed by flow cytometry (BD LSRFortessaTM, BD).
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Immunology 2022Quote: ... 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences). Medium or stimulation with α-CD3 and α-CD28 (both 1 µg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were subjected to flow cytometry to measure mCherry and EGFP (BD Accuri C6). In one experiment ...
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bioRxiv - Molecular Biology 2020Quote: ... Retrotransposition (EGFP+ cells) was quantified 8 days post-transfection in a FACS Canto cytometer (BD).
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bioRxiv - Molecular Biology 2020Quote: ... following which the number of EGFP-positive cells was measured using FACSCanto II (BD Biosciences) (30,000 cells per biological replicate were analysed) ...