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Citations for Becton, Dickinson and Company :
351 - 400 of 1553 citations for hsa mir 483 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated with an intracellular antibody panel for 30 min at RT in 1x permeability/wash buffer (BD #554723), including NeuN-APC (1:50) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized with BD Fix/Perm for 20 min at room temperature (RT) and stained intracellularly with antibodies in 1x Perm buffer (BD Biosciences): anti-IFN-γ (1:100 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and washed with ice-cold PBS twice and resuspended in annexin V binding buffer (#556454 BS bioscience) and incubated at RT in the dark with 7-amino-actinomycin D (7-AAD, #559925, BD bioscience) and annexin-V for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Final PCR products were purified with AMPure Beads (BD Bioscience). The library was prepared with PCR products pooled in equimolar amounts following the manufacture’s protocol and loaded in a Micro MiSeq Reagent Kit v2 (500-cycles ...
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bioRxiv - Cell Biology 2023Quote: ... Final PCR products were purified with AMPure Beads (BD Bioscience). The library was prepared with PCR products pooled in equimolar amounts following the manufacture’s protocol and loaded in a Micro MiSeq Reagent Kit v2 (500-cycles ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were purified with AMPure Beads (BD Bioscience, US). For the second (PCR2) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times and analyzed with a BD FACS Melody machine (BD Biosciences).
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bioRxiv - Microbiology 2019Quote: ... Plates were washed 7 times and developed with TMB substrate according to the manufacturer’s instructions (BD). The reaction was stopped with 2M sulfuric acid and absorbance read at 450 nm with a Synergy H1 micro plate reader (BioTek).
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bioRxiv - Immunology 2020Quote: ... 0.4% formaldehyde) diluted by 20 times prior to analysis on BD FACSCanto™ II (BD Biosciences).
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bioRxiv - Microbiology 2021Quote: ... The cells were washed for 3 times gently and stained with propidium iodide (BD Bioscience, 556463) and then analyzed with fluorescence microscopy.
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bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were washed three times with 1X Perm/Wash solution (BD Biosciences cat.#51-2091KZ) and then incubated with diluted primary antibody in 1X Perm/Wash solution overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Samples were collected at the time of donation using serum separator tubes (BD, Franklin Lakes, NJ), centrifuged ...
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bioRxiv - Bioengineering 2022Quote: ... washed three times with PBS and analyzed using a BD Accuri C6 flow cytometer (BD Biosciences). Median Fluorescence Intensity (MFI ...
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bioRxiv - Molecular Biology 2024Quote: ... homogenized three times with a needle (BD Microlane 3, No. 14, 0.6 x 30 mm, blue), centrifuged (13000 rpm ...
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bioRxiv - Developmental Biology 2022Quote: Zebrafish wild-type and vsxKO retina sections from different developmental stages were analysed for the detection of apoptotic and mitotic cells using rabbit anti-active caspase-3 antibodies (BD Biosciences, 559565) and rabbit anti-phospho-histone H3 antibodies (Merck Millipore ...
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bioRxiv - Bioengineering 2020Quote: Jurkat activation during anti-CD3 administration or co-culture was assessed using flow cytometry detection of CD69 with an APC-conjugated anti-CD69 antibody (BD Bioscience 555533). Cells were not washed prior to or after staining ...
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bioRxiv - Immunology 2022Quote: ... and the supernatants were harvested for cytokine detection (IL-10 and TGF-β) by specific ELISAs using a standard protocol (BD OptEIA). The detection limits for IL-10 is 31.3–2000 pg/ml e TGF- β is 62.5–4,000 pg/mL) ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Lentivirus was titrated by serial dilution of lentivirus and incubating with 5000 HEK293T cells in 96 well plates with 8 µg/ml polybrene for 3 days followed by GFP detection by flow cytometry (BD Biosciences LSRFortessa). Transduction units (TU ...
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bioRxiv - Immunology 2020Quote: ... Cellular depletion in spleens and intraepithelial lymphocyte compartments was monitored by flow cytometric detection with α-CD4 BV650 (clone RM4-5, BD Biosciences) or α-CD8β (clone H35-17.2 ...
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bioRxiv - Immunology 2021Quote: ... and apoptotic DCs after treatments were determined by flow cytometry using surface Annexin V detection and 7-Amino-actinomycin D (7-AAD) incorporation (BD Biosciences, USA). DCs were washed with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The detector settings for fluorescence detection on the CytoFLEX S and LSR Fortessa were determined using the PE MESF beads (QuantiBrite, BD Biosciences, USA). On the Fortessa ...
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bioRxiv - Microbiology 2023Quote: ... and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212, RRID: AB_2869042; BD Biosciences, Heidelberg, Germany). Signals were detected by Streptavidin-PE (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ produced upon re-activation of peptide-specific T cells and captured on anti-IFNγ capture antibody was visualized via sequential addition of 2 μg/mL biotinylated anti-IFNy detection antibody (BD #51-1818KA), Streptavidin-Horseradish Peroxidase (HRP ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed in perm/wash solution, followed by intracellular staining (30 min, RT) using a cocktail of the following antibodies: IFN-γ (BD, 557998)/IL-2 (BD ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... After washing with 1% BSA-PBS slides were incubated with secondary goat anti-rat and goat anti-rabbit biotinylated antibodies for 30 min at RT followed by 30 min of incubation with streptavidin–HRP (1:500; BD Pharmingen, 554066). For developing DAB substrate (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Plates were then incubated in the dark at RT for 15 minutes and analyzed via flow cytometry using BC Fortessa device (BD Bioscience, USA).
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1h at RT and then stained with EC specific CD31 antibody (BD biosciences, 550274, 1:25 dilution) or muscle base membrane laminin antibody (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed for 1h at room temperature (RT) with a mouse anti-GFP antibody (Living Colors -JL-8, BD Biosciences Clontech) in TBS-T buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... Aliquots from each time point were used for estimation of synchronization and release efficiency by FACS (BD FACS Canto II analyzer and BD FACS Diva v8.0.3 software ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were washed three times with PBS and analyzed by FACSAria II (Becton, Dickinson and Company).
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bioRxiv - Bioengineering 2021Quote: ... and 48h time points in lithium heparin BD Microtainer tubes (Becton, Dickinson and Company, Franklin Lakes, NJ). Tubes were immediately inverted 8-10 times to assure anticoagulation and centrifuged at 4000 g for 3 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were washed two additional times in FACS buffer and analyzed as on a BD LSRFortessa (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... washed 5 times with ice-cold PBS and then resuspended in 1× binding buffer (BD Biosciences, USA) at a concentration of 1 × 106 cells/mL ...
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bioRxiv - Genomics 2021Quote: ... passed through a 26ga syringe five times and re-filtered through a 40um cell strainer (BD Falcon). After centrifugation at 2,000g 5min ...
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bioRxiv - Immunology 2021Quote: ... cells were washed three times in 1X BD Permwash and resuspended in BD stabilizing fixative (BD Biosciences) diluted 1:3 ...
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bioRxiv - Systems Biology 2023Quote: ... At each time point a sample of cells was stained with CD69-PE-cy7 (BD biosciences, USA) and CD226-FITC (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Harvested cells were washed two times with RPMI-1%BSA and fixed with Cytofix Buffer (BD Biosciences). After permeabilization ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 5 times with PBS-T and developed with TMB substrate reagent set (BD bioscience) with development reaction stopped with 1 M H2SO4 ...
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bioRxiv - Immunology 2023Quote: ... Blood (from submandibular; 100 μL) was collected at indicated time-points into serum separator tubes (BD Corporation) and centrifuged at 4,000 × g for 10 min at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... Data were collected from 30,000 cells per time point using a BD FACSCelesta flow cytometer (BD Biosciences) equipped with a 561 nm yellow-green laser ...
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bioRxiv - Cancer Biology 2023Quote: ... Blood was collected at the time of surgery into vacuum tubes containing ethylenediaminetetraacetic acid (EDTA) (BD Bioscience). Cells were isolated by ficoll-density centrifugation and frozen in fetal bovine serum with 5% dimethyl sulfoxide ...
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bioRxiv - Microbiology 2023Quote: ... The presence and complexity of Prochlorococcus populations were monitored over time by flow cytometry (BD/Cytopeia Influx). The seawater base of all media was sterilized by 0.2 µm filtration followed by autoclaving and prepared in acid-washed autoclaved borosilicate glass or polycarbonate tubes.
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bioRxiv - Immunology 2023Quote: Time-lapsed video microscopy was performed using the BD Pathway 855 spinning disk confocal microscope (BD Bioscience), the atto vision software (BD Bioscience ...
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bioRxiv - Immunology 2024Quote: ... mice were euthanized at indicated time points and blood was collected and placed into microtainer tubes (BD) and centrifuged at 9000 rpm to collect sera ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of detection antibody (IFNγ, BD Biosciences, #51-1818KA; IL-17A, Invitrogen, #88-7371-88; and IL-5, BD Biosciences, #51-1806KZ) was added at a 1:250 dilution in ELISpot diluent (eBioscience ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated in the dark at RT with 3 μL propidium iodide (Miltenyi Biotech; cat. 130-093-233) and 3 μL FITC-Annexin V (BD Biosciences; cat. 556419) for 20 minutes and then diluted with 100 μL binding buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Correlation of expression data for the 597 genes in the multiomic mRNA panel generated from K562 cells using the original BD Rhapsody reverse transcription procedure (BD RT; n=1,716) or SuperScript IV RT (SSIV RT ...
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bioRxiv - Biophysics 2019Quote: ... The fixed neurons were rinsed three times with PBS and incubated with anti-β-II spectrin antibody (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: Blood was collected at each time point in citrate cell preparation tubes (BD Biosciences, San Jose, CA, USA) and samples were processed within 2 hours from collection ...