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Citations for Becton, Dickinson and Company :
451 - 500 of 1553 citations for hsa mir 301a 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The specimens were washed in PBS three times and incubated in 1:2000 v/v DAPI (BD Bioscience, NJ, USA) in PBS solution for 5 min.
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bioRxiv - Bioengineering 2023Quote: ... washed two times with FACS buffer (0.3% BSA in PBS) and analyzed using a FACSCanto II flow cytometer (BD Biosciences) until data of at least 70.000 cells per well was acquired ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed four times in PFNC twice and cells were analyzed in a FACS Canto II (BD Biosciences) flow cytometer.
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bioRxiv - Evolutionary Biology 2021Quote: ... colon and fecal samples were homogenized (5 mg/ml in PBS) by vortexing (maximum speed, 5 min., RT) and filtered (BD Falcon™, 40 μm cell strainer # 352340). Larger debris were pelleted by centrifugation (600 g ...
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bioRxiv - Biochemistry 2023Quote: PCR reactions were performed with PrimeSTAR MAX DNA Polymerase (BD Clontech GmbH, Heidelberg, Germany) or Phusion High-Fidelity polymerase (Thermo Fisher Scientific GmbH ...
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bioRxiv - Immunology 2021Quote: ... Samples were washed three times in ice cold PBS supplemented with 2% FBS and sorted on the FACSAria Fusion (BD Biosciences) with Ghost Dye Red 710 (Tonbo Biosciences ...
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bioRxiv - Immunology 2021Quote: ... and MBC populations from May and convalescence time points (donor ages 11-14 years) were sorted using a FACSAria IIIu (BD Biosciences) directly into 1.7 ml eppendorf tubes containing 12.0 μl 1X reaction buffer (Takara ...
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bioRxiv - Immunology 2020Quote: ... cover slips were washed 3 times in 1xPBS and incubated with secondary antibodies (anti-rabbit-AF647, anti-mouse-AF488 (BD Bioscience)) diluted 1:1000 in antibody diluent for 1 hour at RT ...
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bioRxiv - Systems Biology 2019Quote: ... ∼4.5 μg of total tick saliva proteins (in triplicate runs using ∼1.5 μg per run) per feeding time point (24, 36, 48, 72, 96, 120, 144, 168, 192, BD, and SD) were processed for LC-MS/MS as published by our group (32–34).
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bioRxiv - Immunology 2019Quote: ... Cells were then washed three times in FACS buffer and fixed before acquisition on either a LSRII or LSRFortessa (BD Biosciences). For intracellular cytokine staining following restimulation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then washed three times in warmed PBS followed by flow cytometric analysis using a Fortessa flow cytometer (BD Biosciences).
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bioRxiv - Microbiology 2019Quote: ... Cells were then washed three times in PBS followed by incubation in 1% Human Fc Block (BD Biosciences, San Jose, CA) in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed three times to reduce non-specific staining and analyzed on a BD LSRFortessa (BD Biosciences, Franklin Lakes, NJ). To determine levels of expression ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell clusters were washed 3 times with ice-cold HBSS containing 5% FBS and filtered through 100-μm cell strainer (BD Biosciences). The cell suspension containing acini was carefully layered on top of HBSS containing 30% FBS ...
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bioRxiv - Genomics 2020Quote: ... 3 times 5 min washes with TNT were followed by a 1 h incubation with biotinylated goat anti-rat (559286, BD biosciences) 1:100 in TNB ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed three times in ice cold PBS supplemented with 2% FBS and sorted on the FACS Aria Fusion (BD Biosciences) with Ghost Dye Red 710 (Tonbo Biosciences ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were washed 2 times in FCM Blocking Buffer then analyzed on a BD Accuri C6 Flow Cytometer (BD Biosciences).
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bioRxiv - Cancer Biology 2021Quote: ... the samples were rinsed 3 times in FACS buffer and then suspended in FACS buffer for analysis and sorting with FACSARIA III (BD Biosciences). Living single cells were selected via FSC/SSC isolation.
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bioRxiv - Cancer Biology 2021Quote: ... cells with indicated hairpins were cultured for different time points with dox and harvested by trypsinization and fixed with Fix buffer I (BD bioscience) for 30 min ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were then either collected after the indicated treatment time and analyzed for apoptosis via flow cytometry after annexin V/propidium iodide (PI) staining according to protocol (BD Biosciences) or washed in PBS once and seeded in fresh media without drugs to complete 24 hour of incubation (wash out ...
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bioRxiv - Immunology 2022Quote: ... The yeast clones were washed three times with cold PBS+1%FBS before analyzed by FACS using Aria II (BD Biosciences). Positive yeast clones were sorted and used for nanobody cloning.
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bioRxiv - Genomics 2019Quote: ... umbilical cord blood was collected at the time of birth in PAXgene Blood RNA Tubes with the RNA stabilization reagent (BD Biosciences) and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: Cells were harvested using trypsin and washed with PBS 3 times gently and then stained with propidium iodide (BD Bioscience, 556547) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized at room temperature by passing it a few times through a syringe with a 23G needle (BD Biosciences). ES cell RNA was also isolated by directly lysing cell pellets in TRIzol reagent ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cell were washed three times with HF buffer and incubated with secondary antibodies APC-Cy7 goat anti-rabbit IgG (BD, 611203) used at 1:200 dilution and AF647 Donkey anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... cells were washed two times with flow buffer (50 nM EDTA, 5% FBS in PBS) before acquiring them on the FACSymphony A5 (BD Biosciences). The gating strategy is outlined in Suppl Fig 1C.
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times in FACS buffer and fixed before acquisition on either a LSR II or LSRFortessa (BD Biosciences). Data was analyzed using FlowJo v ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed two times and resuspended in 1% paraformaldehyde/1x PBS for collection on a LSR II or FACSymphony flow cytometer (BD Biosciences). Data was analyzed in Flow Jo version 9.9.4.
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bioRxiv - Physiology 2022Quote: ... Sections were washed 3 times in wash buffer then stained for Alexa Fluor 555 mouse anti-E-cadherin (BD, Cat. 560064) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... After 1 h of incubation at 37 °C the cells were washed 3 times with HBSS followed by trypsinization and signal was measured using FACScan and FACSCalibur systems (BD Biosciences). Data was analyzed with FlowJo™ v10 Software (BD Biosciences).
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were then dissociated and washed two times with D-PBS before fixing and permeabilisation for 20 minutes (554714 Cytofix/Cytopearm, BD Biosciences). Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times with PBS to remove the excess of dyes and were acquired on an LSRII (BD Biosciences) flow cytometer.
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Cancer Biology 2023Quote: ... The percentage of sgRNA transduced GFP-positive cell population in culture was measured at indicated time points using a LSRII flow cytometer (BD Biosciences). The change of GFP percentage was used to assess proliferation of sgRNA-transduced cells relative to the non-transduced cells in the culture.
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bioRxiv - Biochemistry 2023Quote: ... The cortex was mechanically dissociated by passing through a glass pipette for twenty times and then filtered through a 70-μm nylon mesh filter (BD Biosciences). Cells were plated onto plastic culture plates coated with 30 μg/ml poly-L-lysine and 2 μg/ml laminin and cultured in Neurobasal Medium containing 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... with the pMH0007-UCOE-pEF1ɑ-Cas9-HA-2xNLS-BFP vector(54) and were sorted two times for BFP-positive cells using a BD FACSAria II Cell Sorter (BD Biosciences) at the Stanford Shared FACS Facility ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were centrifuged and washed with PBS three times before analysis using a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences).
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bioRxiv - Cell Biology 2020Quote: ... TUNEL kit from BD Pharmingen were used for assessment of apoptotic cells following manufacturer’s instruction as described (47 ...
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bioRxiv - Cell Biology 2021Quote: ... Fixation/Permeabilization kit (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... OptEIA ELISA kits (BD) for mouse were used ...
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bioRxiv - Cell Biology 2022Quote: ... washed three times with RPMI and incubated for 30 mins at 37°C in RPMI containing Dispase (BD Cornig, 1:10 dilution) and 30 μg/μl DNase I with continuous and gentle agitation on a rotating shaker ...
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bioRxiv - Immunology 2020Quote: ... 7 and 14) or at 2 time points (days 1and 14) after vaccination into Vacutainer® Heparin tubes (BD Biosciences, Plymouth, U.K.). Blood samples were subjected to immediate preparation and analyses ...
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bioRxiv - Neuroscience 2020Quote: COs were washed 2 times for 5 minutes at room temperature in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Neuroscience 2020Quote: COs were washed 3 times for 5 minutes in 5 mL of PBS in 5 mL round bottom tubes (BD Falcon, #1152367) then fixed overnight in 4% PFA at 4°C on a rocker ...
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bioRxiv - Pathology 2022Quote: ... The resulting suspension of muscle fibres was then disrupted by passing it through an 18-gauge needle 10 times before passing the resulting solution through a 40 μm cell strainer (BD Falcon 352340).
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bioRxiv - Cell Biology 2023Quote: ... at the concentrations indicated in the figures and figure legends and cell survival was assessed at the indicated time points post-treatment by flow cytometry (BD Fortessa X20) after staining with annexin V conjugated to Alexa Fluor plus 647 (ThermoFischer ...
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bioRxiv - Biochemistry 2023Quote: ... 610/20nm emission filter) intensity values as a function of time using a BD LSRII flow cytometer (BD Biosciences, Franklin Lakes, NJ). Data was analyzed using FlowJo software (Treestar ...
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bioRxiv - Genomics 2023Quote: ... then washed three times with DPBS before permeabilization in ice cold Phosflow Perm Buffer III for 10mins at -20°C (BD Bioscience #558050). Before incubation with primary antibodies ...
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bioRxiv - Systems Biology 2023Quote: ... the 21.75 mL of 5M NaCl was gradually delivered to the cells over time using a 20 mL BD syringe (BD™, 309628) mounted on a syringe pump ...