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Citations for Becton, Dickinson and Company :
201 - 250 of 330 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... IL-10 and IL-12p70 were quantified in those culture supernatants by ELISA with BD OptEIA™ human IL-10 and IL-12p70 sets following the manufacturer's instructions (BD Bioscience).
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bioRxiv - Cell Biology 2020Quote: ... The presence of IL-6 in the media was then determined by ELISA using the Mouse IL-6 OptEIA™ ELISA set from BD biosciences (cat ...
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bioRxiv - Immunology 2022Quote: ... Peroxidase-like activity of heme (31) bound to STING1 was detected on the gel after incubation with TMB Substrate Reagent Set (BD Biosciences). After a blue-colored band developing ...
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bioRxiv - Developmental Biology 2022Quote: ... Hbbth3/+ and p21-/-/Hbbth3/+ mice and stained for Ki-67 using the Mouse Anti-Ki-67 Set (BD Biosciences, Cat# 556026). Briefly ...
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bioRxiv - Immunology 2020Quote: The cell supernatant was collected after centrifugation of the cells and the secreted IL2 in the diluted cell culture medium (1:4 dilution) was measured using the IL2 Flex set cytometric bead array (CBA) (BD, 558270) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... APC-AF750 anti-CD38 (Beckman Coulter).Intracellular staining for transcription factors was carried out after cell fixation and permeabilization using the Transcription Factor Buffer Set (BD Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Interleukin-8 secretion of treated THP-1 monocytes was quantified by the ELISA OptEIA™ Set for human interleukin-8 (BD) following the manufacturer’s protocol with optimized wash buffer composition (50x dilution) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then stained overnight at 4°C in 1X perm/wash buffer with FITC mouse Ki67 set (BD Pharmingen #556026). Cells were then washed with 1X perm wash buffer and re-suspended in PBS supplemented with 1ug/ml 4′,6-diamidino-2-phenylindole
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bioRxiv - Immunology 2021Quote: ... Cells were fixed and permeabilized for intracellular staining with the FoxP3/Transcription Factor Staining Buffer Set (eBioscience) prior to staining with FoxP3-Alexa Fluor 488 (259D, BD Biosciences) (Table E2 ...
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bioRxiv - Microbiology 2020Quote: ... cell debris pelleted by centrifugation (1400 x g for 10 min at 4°C) and supernatants assayed for chemokine and cytokine levels using the CBA flex set (BD Bioscience) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Intracellular stains were performed using anti-T-beteFluor66O (clone 4B10; eBioscience) in conjunction with a Transcription Factor Buffer Set (BD Biosciences). Samples were acquired using an LSR Fortessa or a FACSCanto II (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: Supernatants were collected after 4 or 24 h and the levels of TNF and IL-1β were quantified by ELISA using OptEIA human TNF ELISA Set (BD Biosciences) and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolation of CD4+ T cells was carried out by negative selection using the Human CD4 T Lymphocyte Enrichment Set-DM (BD Biosciences). All patient samples were obtained with informed consent for research use ...
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bioRxiv - Cancer Biology 2022Quote: ... primary splenocytes were collected from C57BL/6J mice and CD4+ T cells were then isolated with the Mouse CD4 T Lymphocyte Enrichment Set-DM (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... cells were stained for surface antigens as above and then fixed and permeabilised with the Transcription Factor buffer set (562725, BD Biosciences). Next cells were incubated with 4M HCL for 10 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Compensation controls were obtained by incubating each single antibody with the anti-mouse Ig,k/Negative Control Compensation Particle Set (CompBeads, BD Biosciences). Fluorescence Minus One controls (FMO ...
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bioRxiv - Neuroscience 2022Quote: ... were quantitatively determined in spinal cord homogenates by flow cytometry analysis using the mouse Cytometric Bead Array (CBA) Th1/Th2/Th17 kit and specific mouse Flex set systems (BD biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Circulating levels of IL-6 and TNF were determined using a cytokine bead array (BDTM Cytometric Bead Array Mouse IL-6/TNF Flex Set, BD Biosciences). Samples were analyzed in technical triplicates.
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bioRxiv - Immunology 2023Quote: ... The supernatants were collected and frozen for the quantification of cytokine levels with the Flex Set Cytometric Bead Array (CBA; BD Bioscience) using the supplier’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... MPP1-4 from WT and mutant mice were permeabilized and fixed according to the manufacturer’s instructions with the FOXP3 permeabilization kit (Foxp3/Transcription Factor Staining Buffer Set) and then labeled with Ki-67 Ab (clone B56, mouse IgG1; BD Biosciences) and DAPI before flow cytometric analysis ...
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bioRxiv - Immunology 2023Quote: ... Flow cytometry was performed using mock infected LOs as a negative control and gates were set to exclude the dead cells (Zombie UV stained) using the LSR Fortessa X-20 (BD Biosciences). All experiments were done a minimum of three separate times in three technical replicates ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice in PBS-FBS 1% and stained with 20 µL of anti Ki-67 (FITC Mouse Anti-Ki-67 Set, BD Biosciences) for 30 min at RT in the dark ...
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bioRxiv - Immunology 2024Quote: ... This was followed by fixation and permeabilization using either the FOXP3 staining buffer set (eBioscience, 00-5523-00) or the Cytofix/Cytoperm kit (BD, 554714). Intracellular staining was done using the following antibodies ...
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bioRxiv - Biophysics 2023Quote: ... we let bead-PBS solution flow through a set of strainers with mesh sizes of 70 μm (Cell strainer 70 μm Nylon, BD Falcon) and 40 μm (Cell strainer 40 μm Nylon ...
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bioRxiv - Cancer Biology 2024Quote: ... Total BM was depleted of differentiated hematopoietic cells (lineage-positive cells) using Mouse Hematopoietic Progenitor Stem Cell Enrichment Set (BD Biosciences) and HSCs (Lin−Sca+c-Kit+CD34lowFlk2− ...
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bioRxiv - Immunology 2021Quote: ... Flow cytometry was conducted and the supernatant was collected from each well to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... Phospho-STAT1 and phospho-AKT in splenic Tregs were detected using the following cocktail and the Transcription Factor Buffer Set (BD Pharmingen, 562574):CD4-BV650 or CD4-APC (Biolegend) ...
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bioRxiv - Cell Biology 2021Quote: Serum cytokine levels (Ifn-γ, Il-1β and Tnf) were determined with a CBA Flex Set Multiplex Cytokine Bead Array (BD Biosciences).
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bioRxiv - Immunology 2019Quote: ... 1 µL of mAbs were also added to one drop of compensation beads (Anti-Rat Ig, κ/Negative Control Compensation Particles Set (BD Biosciences)) to prepare single-stain controls for compensation ...
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bioRxiv - Immunology 2021Quote: ... and viability dye (eBioscience™ Fixable Viability Dye eFluor™ 780) followed by Foxp3 staining with anti-FoxP3 (259D/C7) using Human FoxP3 buffer set (BD biosciences) according to the Manufacturer’s recommendations.
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bioRxiv - Cell Biology 2021Quote: Cells were treated with 25uM BrdU for 1 hour and BrdU staining detected using FITC Mouse Anti-BrdU Set (BD Biosciences, 556028). Cells were resuspended in 10μg/ml 7-Amino-Actinomycin D (7-AAD ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected after 6 days of co-culture to assess IFNγ and CCL5 levels using a Human IFNγ ELISA Set (BD OptEIA, 555142) and ELISA MAX™ Deluxe Set Human CCL5 (Biolegend ...
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bioRxiv - Systems Biology 2022Quote: ... vortexed and resuspended in 500 μl of 1X SET for same-day cytometric analysis on a BD FACSAria Fusion flow cytometer (BD Biosciences, CA). A gating hierarchy was used to isolate cells of interest ...
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bioRxiv - Microbiology 2023Quote: ... Capture and detection antibodies for human IL-6 were from the OptEIA set for human IL-6 (Cat# 55220, RRID: AB_2869045; BD Biosciences, Heidelberg, Germany) and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212 ...
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bioRxiv - Microbiology 2023Quote: ... and capture and detection antibodies for human TNF were from the OptEIA set for human TNF (Cat# 555212, RRID: AB_2869042; BD Biosciences, Heidelberg, Germany). Signals were detected by Streptavidin-PE (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... IFN-γ and TGF-β in mouse serum was performed with the BD OptEIATM Mouse IL-10 ELISA Set (BD Biosciences, 555252), BD OptEIATM Mouse TNF (Mono/Mono ...
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bioRxiv - Immunology 2023Quote: ... Cytometric Bead Array using enhanced sensitivity flex sets of human IL-2/IFN- γ/TNF- α was done following manufacturer’s instruction (BD Biosciences). Alternatively ...
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bioRxiv - Cell Biology 2023Quote: ... and fixation of the iMACs for 15 min at RT in BD Cytofix buffer (BD Biosciences). Finally ...
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bioRxiv - Immunology 2024Quote: ... samples were either fixed/permeabilized at RT for 15 min with BD Cytofix reagent (BD Biosciences) or alternatively subjected to 15 min of 2% phosphate-buffered PFA prefix102 followed by subsequent overnight fixation/permeabilization at 4°C with the FoxP3 transcription buffer set ...
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bioRxiv - Immunology 2023Quote: ... modifications to the protocol were made: 1 μl of 2 μM additive primer (BD Genomics, beta kit) specific to the antibodies tags was added to the amplification mixture ...
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bioRxiv - Microbiology 2020Quote: ... Assay plates were incubated overnight at 37ºC in a 5% CO2 incubator and developed using BD ELISPOT AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISPOT reader and Immunospot software (Cellular Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were thawed and sandwich ELISA for human or mouse IFN-γ levels was performed using the BD Human IFN-gamma ELISA Set (BD Biosciences, 555142) or BD Mouse IFN-gamma ELISA Set (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... At the end of the incubation plates were washed 5x and developed with BD OptEIA TMB substrate reagent set (BD, San Jose, CA) for 10 minutes ...
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bioRxiv - Immunology 2019Quote: ... Cytokine concentration was quantified in the supernatants by cytometric bead array (CBA) according to the manufacturer’s instructions from the Mouse/Rat Soluble Protein Flex Set System kit (BD Biosciences Pharmingen, Oxford, UK). CBA data was analysed on a MACSQuant analyser with MACSQuantify™ Software and the CBA analysis software package (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... reported to be secreted by DCs (Morelli et al., 2001) was selected to measure in the supernatant of the MutuDC by Cytometric Bead Array (BD CBA Flex Sets) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... and interleukin-2 cytokine production was measured using BD optiEA Mouse IL-2 ELISA set following the manufacturer’s instructions (BD Biosciences, Cat no. 555148).
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bioRxiv - Neuroscience 2023Quote: ... and was used to set the gating for fluorescence activated cell sorting for additional nuclei purification (BD Aria II, 100µm nozzle, BD Biosciences). After FACS ...
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bioRxiv - Cell Biology 2023Quote: ... Fresh samples of peripheral blood from healthy adult volunteers were collected via a 23-gauge butterfly needle collection set (BD #23-021-022) into 10 ml Vacutainer EDTA tubes (BD #366643) ...
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bioRxiv - Molecular Biology 2020Quote: ... for 1h at RT and probed with specific primary antibodies (α-p21 (1:500, BD Biosciences, 556430), DIS3L (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 5 min at RT prior to add a 50 μl droplet of collagen type I (BD bioscience - 50:1 ratio unlabeled vs Alexa548-labeled ...