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Citations for Becton, Dickinson and Company :
151 - 196 of 196 citations for WesternBright Quantum HRP Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the plates were revealed by incubation for 30 min with streptavidin HRP-conjugated (BD Biosciences) as described above.Polyreactivity ELISA was performed as previously described (Planchais et al. ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgG1 was detected with HRP-conjugated corresponding detection antibodies (anti-IgG1 (BD, 553441) with TMB peroxidase substrate (T3405 ...
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bioRxiv - Immunology 2021Quote: ... Wells were extensively washed again and developed with 3-amino-9-ethyl-carbazole (AEC) substrate set (BD Biosciences). After drying ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of enrichment culture of each substrate was spread on a Marine Agar 2216 plate (MA, BD), and the culture plate was maintained at 15°C for 5 days under anaerobic conditions until many visible colonies were observed ...
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bioRxiv - Immunology 2023Quote: ... plates were washed again with PBS+Tween-20 0.05% followed by the addition of TMB substrate (BD, 555214). Plates were allowed to develop for 10-25 minutes before adding 1M H2SO4 stop solution ...
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bioRxiv - Immunology 2020Quote: ... Immunoglobulins were detected with horseradish peroxidase (HRP) goat anti-mouse Ig (BD Pharmingen, 1/2000 diluted), IgM ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed with PBS prior to the addition of 100 μL/well TMB substrate (BD Biosciences). Fifteen minutes after the addition of substrate ...
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bioRxiv - Immunology 2023Quote: ... Spots corresponding to antigen-specific antibody-secreting cells were developed using BD ELISPOT AEC Substrate Set (BD, Cat.#551951) at room temperature for 15 min ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed six times with PBS-tween and developed by adding 50 μl/well of tetramethylbenzidine substrate (BD), with reaction stopped using 1.5M H2SO4 ...
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bioRxiv - Immunology 2023Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary Ab (BD Biosciences, San Diego, CA, USA). Chemiluminescence detection was performed with the ECL Prime Western Blotting System and acquired by the ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary Ab (BD Biosciences, San Diego, CA, USA). Chemiluminescence detection was performed with the ECL Prime Western Blotting System and acquired by the ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... 10 washes in PBS-Tween 20 0.05% and the reaction was visualized by the addition of chromogenic substrate (TMB, BD Biosciences). A stop solution (H2SO4 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA, and BD OptEIA Substrate Solution ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher, for RBD-ELISA,and BD OptEIA Substrate Solution ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Developmental Biology 2021Quote: ... The MEF-free hESC clumps were suspended in a MEF-conditioned medium and seeded onto a Matrigel substrate (BD Biosciences). The culture medium containing bFGF was refreshed daily until the next passage.
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bioRxiv - Immunology 2023Quote: ... The plates were then washed with PBS prior to the addition of 100 μL/well TMB substrate (BD Biosciences, #555214). Fifteen minutes after the addition of substrate ...
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bioRxiv - Immunology 2020Quote: ... Bound CBS004 and AC144 were detected by secondary antibodies: Mouse Anti-Human IgG (anti-CH1-HRP) (BD Pharmigen) at 1:1000 or Peroxidase conjugated Affinipure Donkey anti-mouse IgG (anti-mouse IgG-HRP ...
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bioRxiv - Molecular Biology 2019Quote: ... Chemerin was detected using biotin-conjugated rat-anti mouse chemerin mAb (BAM2325) followed by streptavidin-HRP (BD Science). The reaction was developed with TMB substrate (BD Science) ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed twice with PBS-Tween and twice with 1x PBS before being developed with OptEIA TMB Substrate (BD Biosciences). Reaction was quenched with 2N hydrochloric acid and plates were read at 450nm ...
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bioRxiv - Systems Biology 2020Quote: ... Both dried sides of PDMS and glass substrates underwent with plasma cleaning by hand-held corona treater (Electro-Technic, BD- 20) for 2 minutes ...
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bioRxiv - Immunology 2022Quote: ... Peroxidase-like activity of heme (31) bound to STING1 was detected on the gel after incubation with TMB Substrate Reagent Set (BD Biosciences). After a blue-colored band developing ...
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bioRxiv - Immunology 2022Quote: ... Addition of 100U RNase HII lead to cleavage of positive control BK as well as substrate BD with fluorescence reaching a plateau (BK plateau and BD plateau) above the fluorescence levels of positive controls B and BK ...
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bioRxiv - Immunology 2020Quote: ... The bound antibodies were detected with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:4000) for 1 hour at 37 °C and developed with tetramethyubenzidine (TMB) substrate (BD PharMigen), followed by measuring the absorbance of individual wells at 450 nm using an ELISA reader (Bio-rad mod.550) ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis was measured by evaluating the activation of caspase 3/7 using the Ac-DEVD-AMC Caspase-3 Fluorogenic Substrate (BD Biosciences) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, for the RBD ELISA, and BD OptEIA Substrate Solution ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... which was incubated for 2 h in the dark at 37°C with 20 µM caspase-3 substrate Ac-DEVD-AMC (BD Biosciences Pharmingen) or 2 ml from a stock of 1 mg/ml for a final volume of 150 µl ...
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bioRxiv - Cell Biology 2023Quote: ... Protein blots were visualized using Western ECL Substrate Kit (Thermo Pierce) and exposed using a ChemiDoc XRS+ system (BD, Franklin Lakes, NJ). The intensity of bands was quantified by Image J software
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bioRxiv - Immunology 2021Quote: ... HRP-conjugated anti-mouse IgG (SantaCruz, Cat no. sc-2005) and anti-rabbit IgG (BD Pharmingen, Cat no. 554021) were used as secondary antibodies.
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bioRxiv - Microbiology 2021Quote: ... 100μl of diluted secondary antibody was added to each well with 1:2000 dilution of HRP-conjugated Streptavidin (BD) and incubated for 1 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... biotinylated antibody was detected with Streptavidin-HRP (1:1’000 in 1% skim milk in 0.1% PBS-T, BD Biosciences). Chromogenic reaction and reading of plates were performed as described in (24) ...
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bioRxiv - Immunology 2022Quote: ... followed by washing and incubation for 30 mins at room temperature with horseradish peroxidase (HRP)-streptavidin (BD, Cat. 554066) and Amplex red substrate for 45 mins at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... After incubation with appropriate horseradish peroxidase-conjugated secondary antibodies (HRP-linked anti-mouse or anti-rabbit IgG; BD Biosciences) at RT for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Assay plates were incubated overnight at 37ºC in a 5% CO2 incubator and developed using BD ELISPOT AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISPOT reader and Immunospot software (Cellular Technology ...
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bioRxiv - Immunology 2022Quote: ... At the end of the incubation plates were washed 5x and developed with BD OptEIA TMB substrate reagent set (BD, San Jose, CA) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... wells were washed 6 times as before and HRP activity was detected using TMB (3,3’, 5,5’ tetramethylbenzidine) reagent (BD OptEIA™) according to the manufacturer’s instructions (incubating for 15 −30 min) ...
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bioRxiv - Biophysics 2022Quote: ... Lipid Strips analyses were performed with GFP specific first antibodies and detected with a goat anti-mouse HRP-linked secondary antibody (BD) and ECL reagent (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... the plates were washed and incubated in a 1:2000 dilution of HRP Goat Anti-Mouse Ig (BD, cat. #554002) in PBS with 10% nonfat milk and 0.03% Tween-20 ...
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bioRxiv - Immunology 2023Quote: ... were incubated for 1 h at room temperature followed with another incubation of diluted HRP-conjugated streptavidin (554066, BD Biosciences) for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Assay plates were incubated 24-48 h at 37⍰°C in a 5% CO2 incubator and developed using BD ELISpot AEC substrate set (BD Biosciences, San Diego, CA). Spots were counted and analyzed using an ELISpot reader and ImmunoSpot®software v6 (Cellular Technology ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated control peptides were exchanged with HLA-DR at 1:2 molar ratio in the presence of 5 times excess of a test peptide and detected using horseradish peroxidase (HRP)-conjugated streptavidin (BD Biosciences). PBMCs obtained after vaccination from each donor were stimulated with 47 DR-binding peptides at 0.4ug/ml per peptide for 3 weeks and stained with tetramers ...
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bioRxiv - Cell Biology 2022Quote: ... for epitope retrieval and incubation with primary anti-Collagen Type II (DSHB II-II6B3) followed by a biotinylated secondary and Streptavidin-HRP (BD Biosciences). II-II6B3 was deposited to the DSHB by Linsenmayer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... After washing with 1% BSA-PBS slides were incubated with secondary goat anti-rat and goat anti-rabbit biotinylated antibodies for 30 min at RT followed by 30 min of incubation with streptavidin–HRP (1:500; BD Pharmingen, 554066). For developing DAB substrate (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody incubated for one hour at RT in 5% milk TBS-T at 1:1,000 (HRP-Anti-Mouse, BD Pharmigen, Goat Polycloncal, 554002). Between steps 3 × 5 minute washes were performed in TBST ...
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bioRxiv - Immunology 2020Quote: IgG subclasses were measured from day 21 sera using the same ELISA protocol with the following secondary Abs: goat anti-mouse IgG1-HRP (BD Biosciences, San Jose, California), goat anti-mouse IgG2b-HRP (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... Antibody dilutions were made in 5% (w/v) bovine serum albumin (α-GST, α-His, α-HA, α-biotin-HRP, α-MLO2) in TBST or 5% (w/v) milk (α-Gal4 BD, α-Gal4 AD ...