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Citations for Becton, Dickinson and Company :
51 - 100 of 169 citations for Water RNase Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... Following erythrocyte lysis (10 x BD FACS Lysing solution diluted with sterile water, BD Biosciences, Lyngby, Denmark), leukocyte permeabilization (Fixation/Permeabilization buffer ...
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bioRxiv - Microbiology 2023Quote: Bacteria were isolated from site #1 by plating water on Zobell Agar (1 g yeast extract (BD), 5 g bacto-peptone (BD) ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was removed and cell pellets were washed twice with PBS before resuspending in 0.2-0.5 ml of propidium iodide (PI)/RNAse staining buffer (BD Pharmigen) followed by incubation at room temperature for 15 minutes in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed with −20°C 75% ethanol overnight and then stained with PI/RNase staining buffer (BD pharmingen) for 15 minutes at room temperature and analyzed with flow cytometry (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: The cell cycle assay was performed with the PI/RNase Staining Buffer Kit (BD Biosciences, San Jose, CA, USA). A total of 3 × 105 cells were seeded into each well of 6-well plates and allowed to attach overnight ...
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bioRxiv - Genomics 2020Quote: Subjects collected stool samples at home using DNA/RNA-free sterile swabs (BD BBL CultureSwab Sterile/Media-free Swabs ...
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bioRxiv - Cell Biology 2020Quote: ... After 2 additional days free-floating EBs were transferred on Matrigel (BD Biosciences) coated plates and cultured in neural medium (DMEM/F12-GlutaMAX containing 2% B27 with Vitamine A ...
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bioRxiv - Microbiology 2020Quote: ... recipe (%weight/volume) in 18mM sodium hydroxide in Milli-Q water pH 6.6 +/- 0.05% Tween-80: 0.03% BactoCasitone (BD Science), 0.005% ferric ammonium citrate (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... Wheat flour samples were resuspended with water and filtered through a 100 μm cell strainer (BD Falcon #352360). Samples were then centrifuged at 3000g for 5 min ...
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bioRxiv - Immunology 2023Quote: ... Left lung lobes were homogenized in 3 mL MilliQ water containing PANTA™ Antibiotic Mixture (BD, cat.no. #245114) using GentleMACS M-tubes (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... and bead concentrations from the water samples were estimated using the Accuri C6 Plus flow cytometer (BD Biosciences). Each sample was run for 1 min at a flow rate of 14 µL min-1 ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were finally washed twice in TBS + 3% BSA and resuspended in PI/RNase staining buffer (BD Pharmingen, ref. 550825). All centrifugation steps were performed at 500 g for 1 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... equal amounts of cell lysate premixes were pipetted to another flat-bottomed 96-well reaction plate containing 100 μl reaction buffer with 270 nM type 2 RNase H-specific substrate (BD) using a multi-channel pipette ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed and resuspended in PI staining buffer (50 μg/ml propidium iodide and 0.2 mg/ml RNase A in PBS) and analysed on a FACSCanto II cell analyser (BD Biosciences) and FCS Express 7 (De Novo Software).
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bioRxiv - Genomics 2023Quote: ... were permeabilized in PBS (shark PBS or PBS) containing 0.1 % Triton-X100 and stained with propidium iodide (PI/RNase Staining Buffer, BD Pharmingen).
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bioRxiv - Cell Biology 2023Quote: Cells were analyzed for cell cycle phase using a ClickIT EdU Alexa Fluor 647 kit (Fisher) and FxCycle PI/RNase Staining Solution (Fisher) followed by flow cytometry on an LSR-II (BD) and analysis using FlowJo.
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bioRxiv - Neuroscience 2023Quote: ... and resuspended the cells in 0.3 ml of cold PBS + RNase inhibitor for sorting in a FACS Melody cell sorter (BD Biosciences).
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bioRxiv - Cell Biology 2023Quote: Cell cycle and cell apoptosis assays were performed according to the manufacturer’s instruction (FITC Annexin V Apoptosis Detection Kit I, BD, 556547; PI/RNase staining buffer, BD, 550825).
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bioRxiv - Plant Biology 2023Quote: ... The suspension was then incubated in propidium iodide and RNase for 1 h and detected with flow cytometry (BD FACScalibur). Tomato and barley were used as internal references.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were prepared in antibiotic-free media and mixed with Matrigel (BD biosciences, # 356237) at a 1:1 ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were fixed in formalin-free immunohistochemistry zinc fixative (BD Pharmingen, San Diego, CA) and 4 µm paraffin sections obtained ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated organoids were mixed with 50 μl of phenol-red-free Matrigel (BD Biosciences) and seeded in 24-well plates ...
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bioRxiv - Neuroscience 2020Quote: Thirty-micron free-floating striatal sections were stained for αsyn (Syn1, BD, 1:300) and Alexa Fluor Plus 647 secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated overnight at 37°C and then bacteria were harvested by adding 1.0 ml of Buffered Peptone Water (BPW, BD) to the surface of the plate and gently scraped using an L-shaped plate spreader ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted to a maximum final concentration of 0.55×106/ml in MilliQ water and filtered through a 40 μm filter (BD). EQ beads were added (10% by volume ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the resulting egg rafts were placed in 27.0 × 19.4 × 9.5 cm plastic pans with 800ml of dechlorinated tap water and were supplied with 0.16g of liver powder (BD DifcoTM Desiccated ...
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bioRxiv - Plant Biology 2023Quote: ... Gravimetric water content was then converted into VWC by multiplying it by the bulk density (BD, Mg m−3), assuming that water density is 1.0 Mg m−3 ...
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bioRxiv - Biochemistry 2023Quote: ... and separated by HPLC with the following parameters (system B = 50 % water, 50 % acetonitrile, 1 mM ammonium acetate, pH =8.2; column = 00D-437-BD, Luna 3 µm NH2 100 Å LC column 100 x 2 mm ...
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bioRxiv - Physiology 2024Quote: ... #D16-500) dissolved in sterile water (Gavage volume: 200 µL) was administered to mice using insulin syringes (BD, #324911) attached to animal gavage needles (Popper and Sons ...
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bioRxiv - Cell Biology 2021Quote: ... freshly isolated primary HCs or cultured HCs collected after ploidy transition were fixed at 2×106/ml in 75% ethanol overnight at −20°C.Cells were then incubated with 500 μl of PI/Rnase Staining Buffer (550825, BD Pharmingen, USA) at room temperature for 15 min and analyzed by flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: ... and 0.1 mg/mL RNase A (Thermo Scientific, EN0531. Data were collected with a Accuri C6 cytometer and software (BD Biosciences). Data analyses were performed using Flowjo v10 software.
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bioRxiv - Cell Biology 2023Quote: ... The nuclei were washed again and resuspended in sorting buffer with 0.2 U/μl Protector Rnase inhibitor prior to cell sorting to separate the PCM1-labeled fractions using a FACS cell sorter (BD Aria). The sorted nuclei were then pelleted at 3000 × g for 10 minutes before mRNA isolation using the Direct-zol RNA Microprep kit (Zymo research R2060) ...
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bioRxiv - Neuroscience 2023Quote: ... we resuspended the cells in 0.6 ml of cold PBS + RNase inhibitor and then filtered the cells with 100-μm cell strainers (Falcon brand, BD Biosciences). Cells were then incubated with fluorophore-conjugated primary antibodies against NeuN and Fos ...
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bioRxiv - Genomics 2020Quote: ... iPSCs were transitioned to a ‘feeder-free’ state by culturing on Matrigel-coated wells (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... and fully supplemented RPMI with ice-cold matrigel (Matrix High Concentration, Phenol Red-Free, BD Biosciences ...
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bioRxiv - Bioengineering 2020Quote: ... Both lines were cultured in feeder-free media on growth factor reduced Matrigel (BD Biosciences) and fed daily with mTeSRTM-1 medium (STEMCELL Technologies)45 ...
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bioRxiv - Plant Biology 2020Quote: Isoelectric focussing (IEF)-free-flow electrophoresis (FFE) was conducted according to the manufacturer’s instructions (BD) in a chamber of 0.5 mm thickness ...
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bioRxiv - Developmental Biology 2021Quote: ... Stem cells were maintained antibiotic free on 1% (vol/vol) Matrigel-GFR (#354230; BD Biosciences) coated dishes at 37°C under hypoxia conditions (10% CO2/5%O2 ...
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bioRxiv - Microbiology 2022Quote: ... Strains were grown on drug-free agar plates (BD BBL™ Chocolate II Agar plates) for 24 hours at 36 ± 1°C in 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... TNF-α and IL-17 were performed in cell-free supernatants using kits from BD-Pharmingen.
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bioRxiv - Immunology 2021Quote: ... Left lung lobes or spleens were homogenized in 3 mL MilliQ water containing PANTA™ Antibiotic Mixture (BD, cat.no. #245114) using GentleMACS M-tubes (Miltenyi Biotec) ...
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bioRxiv - Genomics 2023Quote: ... Pools of ciliate cells (5 – 50 cells) were then sorted into a 384-well plate containing 5 μL of autoclaved source water using FACS (BD FACSMelody™ Cell Sorter ...
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bioRxiv - Developmental Biology 2024Quote: ... Residual buds were dissected down to Stage 3-4 in water using a needle (BD Micro-FineTM IV Insulin Syringes). Inflorescences were moved to fresh media plates and transferred to the growth chamber for at least 24 hours for recovery from dissection ...
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bioRxiv - Microbiology 2024Quote: ... 109 CFU/ml) were ten-fold serially diluted to 1 CFU/ml in 0.1% sterile peptone water (BD, Becton Dickinson). The bacterial concentration was enumerated by spread plating in triplicate 100 μl from serially diluted 10-6 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS and resuspended in 0.5 ml of PBS containing 1 μg/ml propidium iodide (PI) and 50 μg RNase and analyzed by flow cytometry (BD LSR-II) MCF-7 cells were exposed to 10 Gy γ radiation ...
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bioRxiv - Cancer Biology 2021Quote: ... and then fixed in ice-cold 70% ethanol and then re-suspended in stain buffer containing propidium iodide and RNase (BD, Cat # 550825). Apoptosis was assessed using annexin V and propidium iodide staining according to the manufacturer’s instructions (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... and RNase A (80 μg/mL) for 30 minutes and then analyzed using a BD Accuri™ C6 flow cytometer (BD Biosciences). For quantitation of the mitotic fraction ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with PBS and stained using a Propidium Iodide/RNase Staining kit (BD PharMingen. Franklin Lakes, NJ. USA) in the dark at room temperature for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... containing 200 μl of growth-factor-reduced Phenol-red free Matrigel (BD Biosciences, San Diego, CA). Explants ...
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bioRxiv - Cell Biology 2022Quote: ... pre-coated with Phenol-free Growth Factor Reduced 100% Matrigel (25 μl per well, BD Biosciences) and cultured in DMEM-F12 supplemented with 2% Matrigel ...