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Citations for Becton, Dickinson and Company :
401 - 450 of 477 citations for Vitamin D Depleted Serum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... transfected/Dox treated stable cells (5×105 cells) in serum-free media were seeded in the upper chamber of an insert (8 mm pore size; BD Bioscience) coated with Fibronectin (BD Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... 5-10 Spheroids resuspended in serum-free medium were seeded in an insert that has been coated with Matrigel (BD Biosciences). Lower compartment was added with medium containing 20% FBS ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum) followed by staining with anti-mouse CD16/CD32 (mouse Fc block; BD Biosciences) and a decoy tetramer (BV510 ...
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bioRxiv - Cancer Biology 2022Quote: ... were blocked for overnight in 5% goat serum 0.3% Triton in PBS and incubated with 10 µL/mL Alexa 647-conjugated anti-BrdU antibody (BD biosciences, 560209) or anti-cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Immunology 2022Quote: ... For measurements of CXCL13 the cells were cultured for 5 hours at 37°C in RPMI supplemented with 10% human AB serum and GolgiStop (1/1500; BD Biosciences). For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28 ...
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bioRxiv - Microbiology 2022Quote: ... serum from each animal was tested with both a treponemal (FTA-ABS) and a non-treponemal (VDRL; BD, Franklin Lakes, NJ) test to rule out infection with the rabbit syphilis agent Treponema paraluiscuniculi ...
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bioRxiv - Immunology 2020Quote: ... TNF-α and IFN-γ in the serum of experimental monkeys were detected with the Non-Human Primate (NHP) Th1/Th2 Cytokine Kit (BD) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... The enzyme activity was stopped with fetal bovine serum (FBS) followed by the fixation and staining with the APC BrDU Flow Kit – Part A (552598, BD Pharmingen) according to the manufacturer’s recommendation with some exceptions ...
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bioRxiv - Developmental Biology 2019Quote: ... the trypsinised cells were resuspended in ice cold PBS + 10% Fetal bovine serum and subjected to FACS (BD Bioscience FACSAriaTM III). The GFP positive cells were sorted and collected in RP1 buffer and RNA was isolated using Nucleospin RNA XS kit (Macherey Nagel ...
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bioRxiv - Cell Biology 2020Quote: ... Kidney sections were blocked (10% normal goat serum and 1% bovine serum albumin (BSA) in PBS) and incubated overnight at 4°C with primary antibody ((Ly6G, 1:10; BD/Pharmingen), FITC-C3 (1:200 ...
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bioRxiv - Immunology 2020Quote: Cells were washed in PBS and resuspended in PBS containing 2% fetal calf serum and 2 mM EDTA and stained with the extracellular mix using anti-human CD14-BV421 antibody (clone M5E2, BD Bioscience) and anti-human CD36-BUV605 antibody (clone CD38 ...
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bioRxiv - Genomics 2022Quote: ... 106 cells were resuspended in PBS supplemented with 2% fetal bovine serum and incubated with human Fc blocking solution (BD Biosciences) for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 % bovine serum albumin (BSA, Jackson, cat. no. 001-000-162) and 10% Perm/Wash buffer (BD Biosciences, cat. no. 554714) at RT for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 and TGF-beta1 was quantified in serum from immunized and non-immunized mice using the CBA (cy-tometric beads array -BD™) method ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated with serum (2 fold dilutions, 1:20-1:2560) and parasite specific antibody was measured using biotinylated IgG1 (BD Biosciences) or IgG2c (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in staining buffer consisting of 50% PBS containing 1% bovine serum albumin (BSA) and 50% Brilliant Stain Buffer (BD Biosciences) and incubated for 20 minutes at 4°C in the following antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... the thoracic cavity was exposed and 200–400 µL of blood was collected into a microtainer tube with serum separator additive (BD, 365967). The mice were then transcardially perfused with ice cold PBS ...
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bioRxiv - Immunology 2023Quote: ... Single cells were then washed with 1% Bovine serum albumin (BSA) in PBS and blocked with anti-Fcγ receptor (BD Biosciences). Human peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Immunology 2023Quote: ... The proteases were inactivated by adding 10% fetal bovine serum and 2 mM EDTA and the cell disaggregated by passing them through a 40 μm nylon sieve (BD Bioscience). Single cells were then washed with 1% Bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... bilateral subcutaneous dorsal flank tumors were established by injecting 1 × 106 cells suspended in a 1:1 mixture of serum-free medium and Matrigel (BD Biosciences). Once tumors reached an average volume of 100 mm3 ...
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bioRxiv - Biophysics 2024Quote: Human serum was separated from the same whole blood sample along with neutrophil isolation using the standard Vacutainer tubes (Serum Separation Tube, BD, 367989). The serum was aliquoted and stored at a −80 °C freezer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were blocked with 2% normal mouse serum and an anti-CD16/CD32 antibody (FcγR blocker, BD Pharmingen™ 553142) for 30 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 × 106 VCaP cells were subcutaneously injected into the dorsal flank on both sides of each mouse in a 1:1 mixture of serum free medium and Matrigel (BD Biosciences). Once tumors grew to a palpable size (∼400 mm3) ...
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bioRxiv - Cancer Biology 2024Quote: ... mice received a subcutaneous injection of 3 million VCaP cells in a mix of serum-free medium and 50% Matrigel (BD Biosciences). Once tumors grew to about 200 mm³ ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated overnight with hamster anti-CD3ε antibody diluted in 1% goat serum/0.3% Triton-X100/TBS (BD Biosciences, 550277). The next day samples were washed in TBS and incubated at room temperature in goat anti-hamster 647 (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... oxysporum conidia were isolated after growth for 3-4 d in potato dextrose broth (PDB; Becton, Dickinson and Company, Le Pont de Claix, France) by filtering through two layers of Miracloth® (Merck Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... after which the cytotoxicity towards IRF8 WT or KO lymphoma cells was assessed by FACS using 7-aminoactinomycin D (7-AAD) (BD Biosciences, Cat# 51-68981). Controls of non-OVAp pulsed B cell lymphomas were included in all instances ...
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bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...
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bioRxiv - Genetics 2023Quote: ... containing penicillin/streptomycin (50U/ml) and 10% FBS and plated onto 12mm Poly-D-Lysine/Laminin pre-coated coverslips (BD Biosciences, Franklin Lakes, NJ). Cells were incubated overnight at 37°C and imaged the following day (sixteen to twenty-four hours after plating cells ...
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bioRxiv - Cell Biology 2021Quote: Serum cytokine levels (Ifn-γ, Il-1β and Tnf) were determined with a CBA Flex Set Multiplex Cytokine Bead Array (BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... spleen cells were ex vivo stimulated for 20 h with 40 μg/ml PPD (purified protein derivative, Statens Serum Institute, Denmark) in the presence of Golgi Stop™ (BD Biosciences) and Monensin (1000x ...
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bioRxiv - Immunology 2021Quote: ... 100 µL of each collected serum sample diluted 1:100 with 1% (w/v) Difco Skim Milk (cat. n° 232100, BD Biosciences, USA) was added to each plate for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Jurkat T cells were washed once with PBS and incubated in serum-free the RPMI-1640 medium with 5uM Calcein-AM (BD Biosciences-US) at 37 °C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... For stimulation ∼106 lymphocytes plated in medium containing 2% fetal calf serum and 1.5 μl/ml Golgiplug (BD Bioscience; San Jose, CA) were cultured with peptides each present at a final concentration of 2 µg/ml for 5 hr at 37°C in a 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then washed with PBS+2% goat serum and cell cycle analysis was conducted using a BD FACS Calibur (BD Biosciences, UK).
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bioRxiv - Immunology 2021Quote: ... Macaque cells were incubated with 100 μl of FACS buffer (PBS 1x with 2% fetal bovine serum and 1mM EDTA) with human Fc Block (BD Biosciences #564219) at a 1:500 dilution for 30 min on ice.
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bioRxiv - Genetics 2019Quote: ... in PBS with 1 μg/ml bovine serum albumin (BSA and 2 mM Tris-HCl. Cells were analyzed on a BD LSRII flow cytometer (BD Biosciences, Inc) and data were analyzed using FlowJo software ...
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bioRxiv - Immunology 2021Quote: ... cells were blocked in FACS buffer containing 15% normal mouse serum for 15 min and subsequently stained for CD45 (BD biosciences, 564279), CD3 (BD biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... using a 23 G needle and a 1 ml syringe.Blood was collected into a serum separator tube (365967, BD, Franklin Lakes NJ), allowed to clot at room temperature for at least 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed with flow cytometry buffer [PBS + 0.5% Bovine Serum Albumin (BSA) (Fisher BioReagents, BP9706100) + 2 mM EDTA] and incubated at 4°C with Fc Block (BD Biosciences, 553142) for 10 min ...
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bioRxiv - Immunology 2023Quote: The concentration of cytokine IL-12 present in serum samples was evaluated using the “Cytometric Bead Array” kit (CBA, BD Bioscience, USA). The analyses of these cytokines were performed using flow cytometry and the data analyzed using FCAP Array software.
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bioRxiv - Immunology 2023Quote: ... Cells were plated in Tfh conditioning media (RPMI complete media with 10% fetal bovine serum, 10μg/ml anti-IL4 (BD Biosciences, clone 11B11), 10μg/ml anti-IFNγ (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Blood was collected by cardiac puncture from five-hour fasted mice and serum isolated in SST™ Amber tubes (BD Microtainer®). Serum triglyceride contents were determined using the Triglyceride Liquid Assay (Sentinel Diagnostics ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1h at RT and then stained with EC specific CD31 antibody (BD biosciences, 550274, 1:25 dilution) or muscle base membrane laminin antibody (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Blood was collected via cardiac puncture using a 1mL 28G1/2 insulin syringe and added to a serum collection tube (BD Microtainer 365967). Tubes were incubated for 45 minutes at room temperature to facilitate blood coagulation and then spun at 4 °C for 10 minutes (1500 x g) ...
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bioRxiv - Cancer Biology 2023Quote: ... IFN-γ and TGF-β in mouse serum was performed with the BD OptEIATM Mouse IL-10 ELISA Set (BD Biosciences, 555252), BD OptEIATM Mouse TNF (Mono/Mono ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed with FACS staining buffer (PBS supplemented with 2% fetal bovine serum and 1 mM EDTA) and incubated with Fc receptor blocker purified rat anti-mouse anti-CD16/CD32 (BD Biosciences, 553142) for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated for 60 minutes in 1% BSA and 5% goat serum in PBS and then incubated overnight with anti-p21 antibody (BD Pharmingen, mouse, #556431) and anti-p16 antibody (abbiotec ...
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bioRxiv - Microbiology 2021Quote: ... the cryosections were incubated for 1 h in the blocking solution (PBS containing 5% goat serum and 5 μg/mL mouse BD Fc Block™) and then labeled overnight at 4°C with the APC-conjugated rat anti-mouse CD45 antibody (1:100 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... astrocytes were first starved (DMEM without serum) for one hour and subsequently incubated with the 9EG7 antibody (BD Bioscience, 1:20 in DMEM) on ice for 1 h ...