Labshake search
Citations for Becton, Dickinson and Company :
101 - 150 of 1722 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... taken approximately at the time of feed delivery via puncture of coccygeal vessels using two-10mL evacuated tubes with serum separator (BD Vacutainer ...
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bioRxiv - Microbiology 2023Quote: ... We then sheared the flagella by repeated passage through two 5-ml syringes connected by 22-inch-long tubing (ID 0.023”, BD INTRAMEDIC, BD Medical), about 30 times ...
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bioRxiv - Microbiology 2023Quote: ... serial two-fold dilutions of DAP were prepared in a 96-well microtiter plate in Mueller-Hinton broth (BD™) supplemented with Ca2Cl 50 mg/L ...
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bioRxiv - Cancer Biology 2023Quote: ... pHIV-dTomato-CDK19_CasR and pHIV-dTomato-CDK19M_CasR lentiviral constructs and sorted for fluorescence-positive cells (two rounds) using FACS Aria III (BD Biosciences) at the Microscopy and Flow Cytometry Core (MFCC ...
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bioRxiv - Systems Biology 2023Quote: ... All positive AD and BD yeast constructs interacting in the first two phase screenings were organized into a row (BD) and column (AD ...
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bioRxiv - Physiology 2023Quote: ... MLEC were purified after propagating them two times by sorting CD31+/CD144+ cells as described above (anti-mouse CD31-antibody, BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... washed two times with FACS buffer (0.3% BSA in PBS) and analyzed using a FACSCanto II flow cytometer (BD Biosciences) until data of at least 70.000 cells per well was acquired ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: The titles and abstracts of 2 341 articles retrieved from the preliminary search were screened and cross-checked by two independent reviewers (ZL and BD) and discrepancies were resolved through discussion with a third researcher (DM) ...
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bioRxiv - Microbiology 2023Quote: Surface markers of splenocytes were identified using monoclonal antibodies in conjunction with two immunofluorescence analysis with a flow cytometer (BD Accuri C6 Plus ...
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bioRxiv - Genetics 2024Quote: ... FACS-sorting to enrich for Venus+ cells was performed two days after transfection using a FACSAria Cell Sorter (BD Biosciences) as described24 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein blots were visualized using Western ECL Substrate Kit (Thermo Pierce) and exposed using a ChemiDoc XRS+ system (BD, Franklin Lakes, NJ). The intensity of bands was quantified by Image J software
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bioRxiv - Microbiology 2021Quote: ... using nitrocefin (BD Diagnostic systems) as substrate ...
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bioRxiv - Biochemistry 2024Quote: ... special order system (BD Biosciences). Flow cytometry analysis was performed using FlowJo software (TreeStar ...
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bioRxiv - Immunology 2021Quote: ... 2000 cells of the CD45−CD31+CD146+ cell population were sorted into eppendorfs containing 125 μL PBS on one of the two available FACSAria™ II or III machines (BD). After sorting ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated at 1/6 confluence in either six 175 cm2 flasks or in two three-layer 525 cm2 tissue culture flasks (BD Biosciences) and after 24 hours synchronized at different stages of the cell cycle as described in the previous section ...
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bioRxiv - Molecular Biology 2021Quote: ... between 500.000 and 1 million GFP-mCherry double positive cells were sorted each day for 5 consecutive days using two cell sorters (BD FACSAriaII SORP and BD FACSAriaIII). Sorted cells were pooled ...
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bioRxiv - Neuroscience 2021Quote: For this benchmark and the task of age regression we used two Convolutional Neural Networks (ConvNets, sometimes abbreviated CNNs) (LeCun et al. 1999) namely ShallowFBCSPNet (BD-Shallow) and Deep4Net (BD-Deep ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of antibody was quantified in plates coated in triplicate with two-fold dilutions of an appropriate purified mouse Ig isotype standard (IgG and IgA; BD Biosciences), starting at 0.5 μg/μl ...
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bioRxiv - Immunology 2022Quote: ... 0.75 × 105 naïve T cells were cultured for one or two days with plate-bound anti-CD3ε (2 µg/ml, clone 145-2C11; BD Biosciences) in differentiation medium consisting of RPMI1640 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... cells were washed two times with flow buffer (50 nM EDTA, 5% FBS in PBS) before acquiring them on the FACSymphony A5 (BD Biosciences). The gating strategy is outlined in Suppl Fig 1C.
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bioRxiv - Immunology 2021Quote: ... Cells were sorted into two populations: CD45+/CD8+/HLA-DR+ and CD45+/CD8+/HLA-DRnegative in a FACS Aria III (BD Biosciences) with an efficiency above 90%.
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bioRxiv - Immunology 2021Quote: ... The cells were washed two times and resuspended in 1% paraformaldehyde/1x PBS for collection on a LSR II or FACSymphony flow cytometer (BD Biosciences). Data was analyzed in Flow Jo version 9.9.4.
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bioRxiv - Neuroscience 2022Quote: ... the cell suspension was then incubated on two PDGFRα plates coated with rat anti-PDGFRα IgG (BD Biosciences, 558774; 1:500). The adherent cells were washed with PBS to remove all antigen-negative nonadherent cells ...
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bioRxiv - Genomics 2022Quote: ... Polyclonal populations of mCherry-positive cells were selected using two rounds of FACS on a FACSAria II Cell Sorter (BD Biosciences). To generate K562 cells stably expressing Zim3-dCas9 without a fluorescent marker ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by two PBS-wash steps and secondary antibody staining and analysis on an LSRFortessa X-20 flow cytometer (BD Biosciences), which was also used for all downstream flow cytometry.
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were then dissociated and washed two times with D-PBS before fixing and permeabilisation for 20 minutes (554714 Cytofix/Cytopearm, BD Biosciences). Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82 ...
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bioRxiv - Genomics 2023Quote: ... for 5 minutes and stained at a dilution of 1:50 with one of two panels of directly conjugated antibodies for 30 minutes at 4 °C: anti-human CD45 (BD 563792), CD204 (Biolegend 371906) ...
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bioRxiv - Cell Biology 2023Quote: ... with the pMH0007-UCOE-pEF1ɑ-Cas9-HA-2xNLS-BFP vector(54) and were sorted two times for BFP-positive cells using a BD FACSAria II Cell Sorter (BD Biosciences) at the Stanford Shared FACS Facility ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9-BFP+ cells were sorted for top 5% of the brightest GFP signal simultaneously on two BD FACSAria II Cell Sorters (BD Biosciences) equipped with blue 488 nm and violet 405 nm lasers ...
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bioRxiv - Physiology 2024Quote: ... endothelial cells were purified by two consecutive immuno-selection procedures using magnetic beads conjugated with anti-ICAM-2 antibody (BD Pharmingen) and plated into 75 cm2 tissue culture coated flasks ...
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bioRxiv - Immunology 2019Quote: ... Cytokine concentration was quantified in the supernatants by cytometric bead array (CBA) according to the manufacturer’s instructions from the Mouse/Rat Soluble Protein Flex Set System kit (BD Biosciences Pharmingen, Oxford, UK). CBA data was analysed on a MACSQuant analyser with MACSQuantify™ Software and the CBA analysis software package (BD Biosciences).
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bioRxiv - Microbiology 2020Quote: ... Susceptibility testing was performed using Phoenix Automated Microbiology System (BD Diagnostic Systems, Sparks, MD, USA). The isolates were sequenced using a Illumina MiSeq sequencer (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed once with wash buffer 1 and incubated for two days at 4°C with mouse anti-SR antibody (1:1,000; BD Biosciences; cat #612052). Sections were washed with wash buffer 2 (2% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclei preparations were strained through 70 μm cell strainers prior to fluorescence-activated cell sorting (FACS) sorting with a two-laser configuration (488nm 80mW and 640nm 50mW) BD FACSJazz™ stream-in-air cell sorter (BD Biosciences), equipped with a 100 μm nozzle operating at a sheath pressure of 27 psi ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated for 30 min at 4°C with the directly-conjugated antibody and after two subsequent washing steps fixed with icecold 3.7% PFA in PBA and subjected to flow cytometry analysis (BD FACS Canto II). The antibodies used were ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated for 30 min at 4°C with the conjugated antibody in icecold PBS + 0.5% saponin + 2% FCS and after two subsequent washing steps fixed with icecold 3.7% PFA in PBA and subjected to flow cytometry analysis (BD FACS Canto II). The antibody used was ...
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bioRxiv - Immunology 2020Quote: ... After two washing steps cells were resuspended in 400 μl dextramer buffer and analysed in BD FACSVerse™ Flow Cytometer (BD Biosciences). Data analysis was performed using FlowJo (X 10.0.7).
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bioRxiv - Microbiology 2023Quote: ... We then sheared the flagella by repeated passage through two 5-ml syringes connected by 22-inch-long tubing (ID 0.023”, BD INTRAMEDIC, BD Medical), about 30 times ...
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bioRxiv - Cancer Biology 2023Quote: ... was used to combine and load two samples (tumor tissue and corresponding benign tissue) onto a single BD Rhapsody cartridge (BD Bio-sciences). Sample-tag staining was performed according to the manufacturer’s protocol (sample-tag staining at room temperature for 20 minutes and washing by centrifugation at 400 g for 5 minutes) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells with the highest 2-5% GFP signal were selected two to three days post transfection using a flow cytometer (BD FACSAria II). Single cells were cultured in RPMI with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... A pure polyclonal population of dCas9-KRAB expressing cells was generated by two rounds of fluorescence activated cell sorting (FACS) gated on the top half of BFP+ cells (BD FACS Aria). The performance of MCF7 CRISPRi in knocking down endogenous genes was confirmed by individually targeting 3 control genes (ST3GAL4 ...
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bioRxiv - Immunology 2023Quote: ... three lymph nodes (for patients ccp2 and ccp3) and two replicates of PBMC were stained with a panel of fluorescent antibodies: CD45-PerCP/Cy5.5 (BD 564105, clone HI30), CD38-PE (BD 555460 ...
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bioRxiv - Cell Biology 2024Quote: ... 10 menstrual endometrial fragments (diameter 1-2 mm) from donor animals were inserted using two 14G catheters (Insyte, Vialon, BD, Madrid, Spain) under laparoscopic guidance in both left and right flank and behind the uterus ...
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bioRxiv - Immunology 2022Quote: ... Samples were measured in plates using the high-throughput system of a Symphony A3 system (BD). Data was analyzed using FlowJo™ Software V10.6 (BD).
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bioRxiv - Cancer Biology 2019Quote: ... and CellQuest software (BD Immunocytometry Systems). 104 events were analyzed for each sample on FlowJo.
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bioRxiv - Cancer Biology 2019Quote: ... and CellQuest software (BD Immunocytometry Systems). Twenty thousand events were analyzed for each sample on FlowJo.
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bioRxiv - Cancer Biology 2019Quote: ... and CellQuest software (BD Immunocytometry Systems). Ten thousand events were analyzed for each sample on FlowJo.
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bioRxiv - Cancer Biology 2019Quote: ... and CellQuest software (BD Immunocytometry Systems).
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bioRxiv - Microbiology 2021Quote: ... A 1.5-fold testing scale was used to include the standard two-fold antibiotic concentrations and their median values and the cation-adjusted Mueller-Hinton broth (CAMHB - BD, New Jersey, USA) was supplemented with 100 U of catalase (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2019Quote: ... A polyclonal population of cells expressing all 3 fluorescent proteins (mturquoise2, Venus and mCherry) was subsequently generated by two rounds of FACS (BD FACS Aria II).